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Citations for Millipore Sigma :
501 - 550 of 878 citations for Ebola Virus Envelope Glycoprotein GP Zaire Kikwit 95 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Abcam-17785-1-AP) and PSD-95 ((2.5 μg/ml diluted) Abcam-ab12093) using desired concentrations in PBS containing 3% BSA (Sigma), 0.3% Sodium azide (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Enucleated eyes were placed in petri dish filled with carboxygenated (95% O2/ 5% CO2) Ames’ media (supplemented with 1.9g/L sodium bicarbonate, pH 7.4, Sigma Aldrich) and retinas dissected ...
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bioRxiv - Cell Biology 2021Quote: ... Agarose beads were heated at 95°C for 5 min in SDS sample buffer containing 5 % β-mercaptoethanol (Sigma). After SDS-PAGE and transfer to a nitrocellulose membrane with a 0.2 μm pore size (RioRad) ...
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bioRxiv - Neuroscience 2021Quote: ... samples were heated 5 minutes at 95°C and 30µl were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Neuroscience 2019Quote: ... immunolabeled for PSD-95 and analyzed in a fluorescence microscope or detached using accutase solution (catalog no. A6964, Sigma), blocked in PBS-BSA 5% ...
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bioRxiv - Plant Biology 2020Quote: ... Trypsin digestion was stopped either by heating at 95 °C for 3 min or by ultrafiltration using 0.5 ml Amicon Ultra centrifugal devices (20 kDa MWCO, Millipore). A five microliters aliquot was kept for MALDI-TOF MS analysis and the rest was used for the enrichment of biotinylated peptides by affinity chromatography.
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bioRxiv - Biophysics 2022Quote: ... 5 μL of the dye solution was added to 1 mL 5 mg/mL HI (91077C, Sigma-Aldrich, 95%) monomer solution ...
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bioRxiv - Genetics 2022Quote: Fipronil (CAS# 120068-37-3) and Spinosad (CAS# 168316-95-8) were both obtained as analytical standards from Sigma. Insecticide bioassays were performed as described previously [20] ...
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bioRxiv - Developmental Biology 2022Quote: E14.5 mesenephroi with ovaries attached were cultured at 37°C with 5% CO2/95% air on MilliCELL-CM culture plate insert 0.4 μm filters (Millipore) in Dulbecco’s Minimal Eagle Medium ...
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bioRxiv - Microbiology 2019Quote: ... MA-104 (fetal African green monkey kidney epithelial) cells (ATCC CRL-2378.1) were cultivated to 95–100% confluency in Minimum Essential Medium Eagle (MEM, Sigma.), supplemented with 5 % Foetal Bovine Serum (FBS) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed three times in PBS and then fixed in 4% formaldehyde (paraformaldehyde powder, 95%, 158127-2.5KG, Sigma) at room temperature for 15 min ...
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bioRxiv - Bioengineering 2020Quote: ... and eyes were immediately collected and dissected in carboxygenated (95% O2 and 5% CO2) Ames’ medium (A1420, Sigma-Aldrich). Whole-mount retinas were placed ganglion cell down onto the stimulation pixels and transferred to the microscope stage for stimulation and recording ...
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bioRxiv - Microbiology 2019Quote: ... Ethanol (reagent alcohol, specially denatured alcohol formula 3A 95 % and Isopropyl alcohol 5 % by volume) obtained from Sigma Aldrich, USA ...
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bioRxiv - Microbiology 2019Quote: ... 1.2 × 109 bacteria were pelleted, treated with stop-solution (95% EtOH, 5% phenol saturated with 0.1 M citrate buffer, pH 4.3 (Sigma-Aldrich) and snap-frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: Lopinavir (SML1222, purity ≥ 98 %), ritonavir (SML0491, purity ≥ 98 %), and (−)-Epigallocatechin gallate (EGCG) (E4134, purity ≥ 95 %) were purchased from Sigma- Aldrich (Saint Louis ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were fixed overnight with 95% ethanol and after fixation were submerged in Alcian Blue solution (0.03% w/v Alcian Blue (Sigma), 80% ethanol ...
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bioRxiv - Immunology 2022Quote: ... The cell density was kept between 0.2-1.0 × 106 cells/ml with viability over 95% (determined with Erythrosin B (Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for 45 min at 95 °C in 10 mM Sodium citrate (Normapur)/ 0,05% Tween 20 (Sigma) buffer for simultaneous antigen retrieval and permeabilization ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were isolated from the eyecups and placed in oxygenated (95% O2/5% CO2) cold-Ames medium (Sigma-Aldrich), supplemented with 21 mM NaHCO3 ...
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bioRxiv - Molecular Biology 2022Quote: 95 μL of lysed and sonicated equine SF-EV were treated with 5mM dithiothreitol (DTT) (Sigma-Aldrich, Dorset, UK) 100 mM at 60°C and 1000rpm for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for 45 min at 95 °C in 10 mM Sodium citrate (Normapur)/ 0,05% Tween 20 (Sigma) buffer for simultaneous antigen retrieval and permeabilization ...
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bioRxiv - Cancer Biology 2022Quote: ... The aqueous mobile phase A consisted of 95:5 (v:v) water:acetonitrile supplemented with 20 mmol/L ammonium acetate and ammonium hydroxide (Sigma-Aldrich). Mobile phase B was 100% acetonitrile ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were denatured (95 °C for 10 minutes) with 6×sample buffer [0.375 M Tris pH 6.8 (Sigma-Aldrich), 12% SDS (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 45 min at 95 °C with 10mM Sodium citrate buffer (WVR chemicals, #27833.294P) + 0,05% Tween 20 (Sigma, #P1379) for simultaneous antigen retrieval and permeabilization ...
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bioRxiv - Cancer Biology 2024Quote: ... at a final concentration of 20 mM at 95°C for 10 minutes and alkylated with iodoacetamide (Millipore Sigma) at a final concentration of 40 mM at RT for 30 minutes in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... Cellular lipid extracts were analyzed using a 150 mm x 3.2 mm Phosphere C4 column (Grace) and mobile phases (mobile phase A: [95:5:0.1 v/v/v] 10mM Ammonium Acetate (Sigma-Aldrich): Methanol ...
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bioRxiv - Neuroscience 2021Quote: ... Dissected tissues were then incubated in bubbling (95% O2, 5% CO2) aCSF containing 1mg/mL protease from Streptomyces griseus (Sigma) for 15 mins at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Samples were heated for 5 min at 95°C prior to loading on 12% polyacrylamide gels followed by transfer onto NC membrane (Millipore). 3% BSA in phosphate buffer saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... the amount needed for a single day was dissolved in 95% ethoanol with heating to 37°C and then diluted in corn oil (Sigma) such that 100μL had 5mg.
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were eluted in Laemmli buffer by boiling at 95°C for 5min for Western blot or with 3xFlag peptide (150 µg/ml, Sigma) for 1 hr at 4°C for HAT assay ...
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bioRxiv - Microbiology 2019Quote: ... the cell pellet was resuspended in 50% LR White resin (1:1 volume of LR White resin (London Resin Company) and 95% ethanol (Merck Millipore)) and the cell suspension was stored in 4°C till further use ...
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bioRxiv - Neuroscience 2020Quote: ... Eyes were enucleated and immediately placed into carbonated (95% O2/5% CO2) Ames’ medium (A1420; Sigma-Aldrich, St. Louis, MO) containing 1.932 g/L NaHCO3 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... adult flies carrying the Chrimson transgene were placed on cornmeal agar with all-trans-retinal (0.2 mM, dissolved in 95% EtOH, Sigma-Aldrich) for 2-3 days prior to the experiment ...
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bioRxiv - Biochemistry 2020Quote: ... All compounds in the pesticide mixture were purchased as pure technical material (≥95% purity) from Sigma Aldrich (St. Louis, MO) or Chem Service Inc and were serially diluted in acetone in order to achieve the respective concentrations listed in Table 1 ...
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bioRxiv - Plant Biology 2021Quote: ... A total of 1 mL 80% methanol containing 20 μg internal standard was added to the sample (digitoxin, ≥95%, Sigma). The samples were further extracted at 1400 rpm for 2 h and centrifuged at 10,000 g for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... All samples were then boiled at 95 °C for 10 mins at a 1:1 (v/v) ratio in 2x Laemmli loading dye (Sigma) before being electrophoresed through a 4-12% NuPAGE Bis-Tris polyacrylamide gel (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... boiled at 95°C for 5 min and subjected to 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride (PVDF) (Millipore) at 20 V/cm for 1h ...
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bioRxiv - Neuroscience 2020Quote: Amyloid-beta protein fragment 1-42 (Aβ42 peptide) was purchased as a lyophilized solid and more than 95% purity was guaranteed by HPLC from Sigma-Aldrich (High Performance Liquid Chromatography) ...
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bioRxiv - Immunology 2021Quote: ... MS-5 cells cultured in αMEM medium at 95 % confluence were incubated with 10 µg/ml mitomycin C (Sigma-Aldrich) for 3 h at 37° ...
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bioRxiv - Neuroscience 2021Quote: ... euthanized and then retinae rapidly dissected under infrared illumination into oxygenated (95% O2; 5% CO2) Ames solution (Millipore Sigma, A1420). Next ...
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bioRxiv - Neuroscience 2020Quote: ... the retina was dissected at room temperature in Ames’ medium bubbled with 95% O2/5% CO2 (A 1420; Sigma-Aldrich). The separated retina was placed on a cellulose membrane ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were subject to 0.1M citrate antigen retrieval at 95°C for 10 mins before the primary antibody for type-II collagen (Millipore, MAB8887), ACAN [30] or lubricin (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... and high (HGHM, >95%) methylated homogalacturonan were provided from Elicityl Oligotech whereas oligogalacturonides and D-galacturonic acid were provided from Sigma.
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... Samples were dehydrated with methanol series (50%, 75%, 95%, 100%, 100%) and overnight incubated in 1:1 methanol : BABB (BenzylAlcohol-BenzylBenzoate 1:2, both Sigma)) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS twice and then incubated in antibody solution (2% NGS, 0.2% Triton-X-100, PBS) with anti-PSD-95 antibody (Millipore, MABN68) for overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... All samples with SDS sample buffer were heated at 95°C for 5 minutes and reduced with 5% 2-mercaptoethanol (Sigma) as needed.
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were denatured at 95°C for 5mins before being loaded into a TruPAGE 4-20% precast gradient gel (Sigma) and undergoing SDS-PAGE at 120 V for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... were cultured at 37° C and 95%O2/5%CO2 in Dulbecco’s modified Eagle/F-12 medium (DMEM/F-12, Sigma Aldrich), supplemented with 10% foetal calf serum ...
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bioRxiv - Biochemistry 2023Quote: ... Azo biotin-azide (CAS No. 1339202-33-3) and Imatinib (Cas No.152459-95-5) were obtained from Sigma Aldrich, rapamycin (Cas No.53123-88-9 ...
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bioRxiv - Cell Biology 2023Quote: ... and the proteins were then eluted by heating the beads for 5 min at 95 °C in 50 μl of 2x SDS buffer (Laemmli 2x ref S3401-1VL, Sigma), Finally ...