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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Dectin 1 CLEC7A Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... murine fibroblast (3T3, laboratory stocks) and HEK293 (laboratory stocks) cell lines were maintained in Dulbecco’s minimal essential medium (DMEM, Sigma) supplemented with 10% (v/v ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) and K562 cells in RPMI-1640 medium (Sigma-Aldrich). All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Neuroscience 2023Quote: ... were produced in HEK293 cells by mixing lentiviral vector and packaging vectors using branched polyethyleneimine (bPEI) (MW 25Kda, Sigma) as the transfection reagent ...
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Cell Biology 2021Quote: ... 4°C) and the cell pellets washed twice with 10% FCS in PBS and resuspend in Dapi solution (Sigma; 50μg/ml in 10% FCS in PBS; 100μl/well) and stored at 4°C ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... SUM149PT were grown in SUM medium (1:1 mix of DMEM/F12 and Human Mammary Epithelial Cell Growth Medium (Sigma), 5% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Two pLKO.1 lentiviral vectors encoding for specific shRNA for two human MAPK7 mRNA sequences were used (TRCN0000010262/pLKO.1 and TRCN0000010275/pLKO.1, Sigma). Lentiviral particles were generated in HEK-293 cells by co-transfecting the virion vectors (psPAX2 and pMD2G ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with the following primary antibodies for 1 h at room temperature in Abdil: mouse anti-human lamin A/C (1:200; Millipore), rabbit anti-lamin A/C (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... rabbit anti-human βIII-tubulin (1:500; Covance, cat. MRB-435P) and α-tubulin (1:500; Sigma-Aldrich, cat. T9026). Primary antibodies were tagged using either goat anti-mouse AF647 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells isolated from healthy donor PBMCs were stimulated by a 1:1 ratio of anti-CD3/28 activation beads (Miltenyi) in complete human T cell medium (RPMI1640 from Sigma containing 2 mM glutamine with the addition of 1 mM pyruvate ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with the primary antibody OC (1:500 for mouse, 1:200 for human; polyclonal, AB2286, Millipore) and respective secondary antibody (1:400 ...
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bioRxiv - Developmental Biology 2023Quote: ... (gift from the laboratory of Jeremy Nathans) rabbit anti-LW/MW-opsins (1:200 for organoids, 1:500 for human retinas) (AB5405 Millipore), and mouse anti-Rhodopsin (1:500 ...
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bioRxiv - Immunology 2023Quote: ... followed by streptavidin-Cyanine-3 (1:500; Amersham Bioscience) or by goat anti-human IgG coupled to FITC (1:100, Sigma), as appropriate ...
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bioRxiv - Physiology 2023Quote: ... SVG tissue sections were blocked in a phosphate buffered saline solution containing 0.1% NP-40 and 1% BSA followed by overnight incubation in antibodies targeting human VE-cadherin (Catalog No. MABT886, 1:100 dilution, Sigma) or endothelial nitric oxide synthase (eNOS ...
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bioRxiv - Immunology 2022Quote: ... dispersed cells were cultured for 4 h in RPMI/10% fetal calf serum (FCS) with 1.5 ng/ml phorbol 12-myristate 13-acetate (PMA) and 1 μg/ml ionomycin (all from Sigma-Aldrich, St Louis, Mo) with Golgi-Stop (BD Bioscience ...
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bioRxiv - Biochemistry 2024Quote: ... pCD44 was generated by pretreatment of recombinant murine CD44-Fc-AviTag with CK2α in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: ... 10% fetal calf serum or FCS (Sigma-Aldrich, St. Louis, MO, USA) as well as 1% antibiotics (penicillin and streptomycin) ...
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bioRxiv - Cancer Biology 2019Quote: ... with 10% Fetal Calf Serum (FCS) (Sigma-Aldrich, St Louis, MO, USA) and 2 mM L-Glutamine (BE17-605F ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Cancer Biology 2020Quote: ... All media were supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich) and all cells were kept at 37°C with 5% CO2 ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... containing 7 % fetal calf serum (FCS) (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 1% GlutaMAX (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... + 10% fetal calf serum (FCS; Sigma Aldrich, St. Louis, Missouri, United States) on plastic or in PRIME-XV MSC Expansion XSFM medium (FUJIFILM Irvine Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Digested tissue was re-suspended in 10% FCS (Capricorn) DMEM (Sigma Aldrich), Pen/Strep (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Chemotaxis experiments towards 2.5% FCS and 100 ng/ml HGF (#H9661, Sigma) in DMEM were performed with μ-slide chemotaxis chambers (Ibidi ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10% heat inactivated foetal calf serum (FCS; Sigma Aldrich, Germany), 100 U/ml penicillin and 100U/ml streptomycin ...
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bioRxiv - Microbiology 2022Quote: ... an overlay of DMEM containing 2% FCS and 0.6% Methylcellulose (Sigma-Aldrich) was added to the cells ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10 % foetal calf serum (FCS; Sigma - catalogue no. F-7524), 1,000 units/ml LIF ...
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bioRxiv - Immunology 2020Quote: ... The pellet was resuspended in IMDM containing 2% FCS and formaldehyde (Sigma) was added to a final concentration of 1% ...
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bioRxiv - Immunology 2019Quote: ... The pellet was resuspended in IMDM containing 2% FCS and formaldehyde (Sigma) was added to a final concentration of 1% ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 10% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma), 2mM (1% v/v ...
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bioRxiv - Immunology 2021Quote: ... with 10% FCS and 2 mM L-Glutamine (Sigma, Cat No. G7513) and 1% penicillin/streptomycin (Invitrogen cat no ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS superior, Millipore, S0615), 2 mM L-glutamine (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10 % (v/v) FCS (Biochrom, Sigma-Aldrich or PAN-Biotech) and 1 % (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... was mixed with unconjugated Fc-antibody (5 µg/ml, Sigma-Aldrich, # I2136) in HBSS and incubated for 30-60 minutes at room temperature with gentle agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... Hybridoma cells were selected in DMEM/10% FCS containing HAT (Sigma, MO) and 1% BM-Condimed (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich; St. Louise, MO), penicillin streptomycin glutamine (100 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Cancer Biology 2021Quote: ... stained with a 1:40 dilution of anti-human IgG-FITC (Sigma, Mendota Heights, MN) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... IgM was detected using Goat-anti-human-IgM-HRP (horseradish peroxidase) (1:1,000) (Sigma: A6907) and IgG was detected using Goat-anti-human-Fc-AP (alkaline phosphatase ...
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bioRxiv - Immunology 2020Quote: ... and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170, Sigma-Aldrich, USA) respectively ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min at room temperature and blocked in 1% human AB serum (Sigma-Aldrich) for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min at room temperature and blocked in 1% human AB serum (Sigma-Aldrich) in 1X PBS 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... blocked with 100 μl/well of 1% human serum albumin (HAS) in PBST (Sigma-Aldrich) for 1 h at RT and then washed 4 times with 100 μl/well of PBST ...
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bioRxiv - Immunology 2021Quote: ... IgM was detected using goat-anti-human-IgM-HRP (1:1000) (Sigma catalogue no. A6907) and wells were washed with PBS-T and one-step 3,3′,5,5′-tetramethylbenzidine (TMB ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells were transfected with pLKO.1 shRNA plasmid (Sigma, Mission human genome shRNA library) and packaging plasmids-delta 8.9 ...
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bioRxiv - Cell Biology 2019Quote: ... They were coated for 1 hour with 50 μg/mL human plasma fibronectin (Merck Millipore) diluted in PBS and washed three times with PBS.
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bioRxiv - Immunology 2021Quote: ... IgM was detected using Goat-anti-human-IgM-HRP (horseradish peroxidase) (1:1,000) (Sigma: A6907) and IgG was detected using Goat-anti-human-Fc-AP (alkaline phosphatase ...