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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for Dectin 1 CLEC7A Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Nanosota-1C-Fc was cloned into pET42b vector (Novagen) with a C-terminal human IgG1 Fc tag ...
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bioRxiv - Neuroscience 2019Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Sigma-Aldrich), 50 U/ml penicillin (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: hACE2-Fc was digested with thrombin protease (Sigma Aldrich) in the presence of 2.5 mM CaCl2 at a 1:300 w/w thrombin:protein ratio ...
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bioRxiv - Microbiology 2021Quote: ... and 5% FCS (Sigma-Aldrich, St. Louis, MO, USA) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and/or 2.5 μg/ml E-cadherin-Fc (Sigma). IELs were plated in RPMI-T medium containing 2 μg/ml PerCP-e710 conjugated anti-CD107a (1D4B ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma Aldrich), 100 μg/ml streptomycin and 100 U/ml penicillin (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... and 10% FCS (Sigma-Aldrich, St. Louis, Missouri, USA)) assuming that they double within 24 hours ...
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bioRxiv - Immunology 2023Quote: ... mixed in 2% fetal calf serum (FCS; Sigma-Aldrich). Cells were stained with combinations of the following fluorophore-conjugated anti-mouse monoclonal antibodies ...
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bioRxiv - Immunology 2023Quote: ... 10% fetal calf serum (FCS) treated with Chelex (Sigma) to removed endogenous calcium ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich) and 2 mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich), 10 mM HEPES (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cell suspension was incubated with 10% FCS (Sigma) for 30 min to allow the cell membrane to recover from trypsinisation and prevent false positive staining with Annexin V (AV) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10% tetracycline-free fetal calf serum (FCS) (Sigma-Aldrich), 100 U/ml penicillin (Biochrom) ...
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bioRxiv - Genomics 2024Quote: ... containing 10% FCS (heat inactivated) (Sigma-Aldrich, Cat# F7524) and 0.5% penicillin and streptomycin at 27°C.
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% FCS (v/v) (Sigma- Aldrich Ltd), apart from T2 cells which were grown in glutamine containing RPMI (Sigma-Aldrich Ltd) ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma Aldrich). All cells were kept at 37 °C with 5% CO2 and tested for mycoplasma at regular intervals.
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated FCS (Sigma Aldrich #F2442) and 50 ng/ml M-CSF (PeproTech #300-25 ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% fetal bovine serum (FCS, Sigma-Aldrich), 100 U/ml penicillin (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were grown in DMEM (gibco) with 1 g/mL glucose (MCEC) or 4.5 g/mL (HUVEC, HeLa, MCF-7) containing 10% FCS (Sigma), 1% penicillin (10000 Units/ml ...
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bioRxiv - Immunology 2020Quote: ... Intestines were then incubated with 20 mL RPMI + 10% FCS +1% PS + 2.5 U/mL Collagenase VIII (Sigma-Aldrich) + 2 U/mL DNAse I (Roche) ...
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bioRxiv - Immunology 2019Quote: ... Intestines were then incubated with 20 mL RPMI + 10% FCS +1% PS + 2.5 U/mL Collagenase VIII (Sigma-Aldrich) + 2 U/mL DNAse I (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... Inocula were removed and replaced with DMEM containing 1% FCS and a semi-solid overlay consisting of 1.5% carboxymethyl cellulose (SIGMA). Cells were incubated for 72h ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Eurobio Scientific, CVFSVF00-01) and containing 1% penicillin-streptomycin (Sigma-Aldrich, P4333). HEK-293T were grown in Hepes-modified DMEM (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS and 200 ug/ml Digitonin (D141-100MG ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-RBFOX1 (1:2000 for mouse and 1:1000 for human samples, Merk Millipore, MABE985), mouse anti-RBFOX2 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS ...
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bioRxiv - Immunology 2023Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.1% Triton X-100 dissolved in TBS ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35±3 days25 ...
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bioRxiv - Immunology 2020Quote: Cell lines (HEK293T, HEK293, 3C11, 143B, MDCK-SIAT1, MDCK-PR8) were maintained in DMEM (Sigma Aldrich) supplemented with 10% FCS (Sigma Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells overexpressing Flag-AurkB was treated with 10 µM of trichostatin A (TSA) (Sigma-Aldrich) or DMSO for 12 hours ...
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bioRxiv - Bioengineering 2021Quote: ... for HEK293-T and SW480 cells were coated with fibronectin bovine plasma (F1141-2MG; Sigma Aldrich) before seeding cells onto the 8-well plates ...
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bioRxiv - Genomics 2021Quote: ... HEK293 and NSC were transfected with STARR-seq plasmid containing enhancer regions using polyethylenimine (PEI, Sigma) or Lipofectamine™ Stem Transfection Reagent (Thermo Scientific ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were seeded in 8-well Nunc Lab-Tek II chamber slides (0,7 cm2, Sigma) at a density of 70.000 cells/cm2 ...
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bioRxiv - Immunology 2020Quote: ... Protein production was done in HEK293-6E cells by transient transfection with polyethylenimine (PEI, Sigma-Aldrich) and 1 μg DNA per 1 mL cell culture medium at a cell density of 0.8 106 cells/mL in FreeStyle 293 medium (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: 2×105 HEK293 were seeded onto 24well plates previously pre-coated with poly-L-lysine (Sigma), and infected with 2×105 ...
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bioRxiv - Immunology 2020Quote: ... this mixture was applied to mammalian FreeStyle™ HEK293-F cell culture (EMD-Millipore, Darmstadt, Germany) at a density of 1 million cells per milliliter ...
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bioRxiv - Neuroscience 2023Quote: ... and mock plasmids were transfected into HEK293 suspension cells derived from tsA201 (Millipore Sigma, 96121229-1VL) using Polyethylenimine (Polysciences ...
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bioRxiv - Neuroscience 2022Quote: ... for Mouse 448 and human experiments or 1 mg/ml Hoechst (Sigma) for the mouse atlas experiments ...
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bioRxiv - Biochemistry 2021Quote: ... 5% CO2 for 1 hour in human tubular fluid (HTF, EMD Millipore) at 2.0 × 106 cells/ml and co-incubated with oocytes at 2.0 × 105 sperm/ml ...
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bioRxiv - Microbiology 2020Quote: ... blots were reprobed with mouse anti-human tubulin (1:3000, Sigma T5168). SUMO1 smears above 90 kDa (excluding therefore the high intensity RanGAP1 bands present at 80 kDa ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human-LAIR-1 antibody (clone 8A8 (21)) in PBS (Sigma-Aldrich) or isotype control (eBiosciences ...
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bioRxiv - Cell Biology 2021Quote: ... and microprinted with 1:25 diluted 0.1% human serum-derived fibronectin (Sigma).(Grevesse et al. ...
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bioRxiv - Microbiology 2020Quote: Human promonocytic THP-1 cells were maintained in RPMI-1640 medium (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... 100 μg ml−1 streptomycin and 5 % human AB serum (Sigma Aldrich).
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bioRxiv - Evolutionary Biology 2019Quote: ... and 1 mg/ml AAG (human a1-acid glycoprotein, Sigma, Cat # G9885). All medium is sterilized by filtration through a 0.22mm membrane ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were probed with rabbit anti-human Cx43 (1:2000) (Sigma-Aldrich) and rabbit anti-ß-Tubulin antibodies (1:2000 ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit polyclonal antibody against the human ZCCHC10 antigen (HPA038944, 1:2,000; Sigma), mouse monoclonal antibody against the FLAG antigen (F1804 ...