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Citations for Millipore Sigma :
301 - 350 of 5985 citations for DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... USA) was used for the HPLC purification and all solvents used for HPLC purification were of ACS reagent grade (Sigma-Aldrich Chemical Company, St. Louis, MO, USA). The NMR (Nuclear magnetic resonance ...
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bioRxiv - Biochemistry 2022Quote: ... we first extracted the genomic DNA (gDNA) from each cellular fraction using the GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO). A previously described semi-nested polymerase chain reaction (PCR ...
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bioRxiv - Biochemistry 2021Quote: ... Each cellular fraction was then expanded and harvested prior to the extraction of genomic DNA using the GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO). A semi-nested PCR approach was then used to selectively amplify the mutagenized region of the polyprotein reporter from the genomic integration site in a similar manner as was previously described (Matreyek et al. ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNAs were prepared from selected isolates showing different cell and colony morphologies using GenElute™ Bacterial Genomic DNA Kit (Sigma Aldridge, Gillingham, UK). Identification to presumptive species level was carried out by performing PCR amplification of 16S rRNA gene sequences using primers TPU1 and RTU8 (73 ...
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bioRxiv - Genomics 2021Quote: ... genotyping for the Ile148Met variant associated with hepatic steatosis (rs738409) was performed on genomic DNA extracted from the blood buffy coat using the GenElute Blood Genomic DNA kit (Sigma, St. Louis, MO, USA). rs738409 was genotyped using validated primers and TaqMan probes (Applied Biosystems ...
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bioRxiv - Genomics 2019Quote: ... Half of the sample was proceeded for genomic DNA isolation using GenElute plant genomic DNA miniprep kit (Sigma-Aldrich Co., St. Louis, MO, USA) according to the manufacturer’s instructions for PacBio and Illumina platforms sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... Hmga2-/- and doxycycline inducible MEF was extracted using the GenElute DNA Miniprep kit (Sigma-Aldrich) according to the protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... gDNA was extracted from freeze dried mycelia using the GenElute Plant Genomic DNA Kit (Sigma). gDNA was sonicated in a water bath and size selected ...
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bioRxiv - Immunology 2021Quote: ... Bisulfite conversion was performed using an Imprint DNA modification kit (Sigma. Cat. No. MOD50-1KT). Purification and desulphonation of converted DNA was performed with magnetic beads (Zymo ...
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bioRxiv - Immunology 2022Quote: ... Remaining DNA in samples was digested using the amplification grade DNase I kit (Sigma-Aldrich) for 6 min at RT ...
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bioRxiv - Plant Biology 2022Quote: ... (1999) or using the GenElute Plant Genomic DNA Miniprep Kit (Sigma–Aldrich, St. Louis, USA). DNA samples and marker flanking sequences were sent to Floodlight Genomics ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was removed using the On-Column DNase I digestion kit (Sigma-Aldrich, Sweden). cDNA was synthesised using the TATAA GrandScript cDNA Synthesis Kit according to the instructions of the manufacturer (TATAA Biocenter ...
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bioRxiv - Immunology 2022Quote: ... after digestion of the remaining genomic DNA by incubation with the “DNase I Kit” (Sigma) following the manufacturer’s manual ...
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bioRxiv - Microbiology 2022Quote: ... Whole-genome amplification was carried out using a SeqPlex DNA Amplification Kit (Sigma-Aldrich, USA), which is specifically designed for low-quantity ...
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bioRxiv - Developmental Biology 2022Quote: ... Mice were genotyped by PCR on tail genomic DNA using Red-N extract kit (Sigma), with primers indicated in Supplemental table S3.
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bioRxiv - Microbiology 2023Quote: Genomic DNA extraction was performed using Extract-N-Amp(tm) Tissue PCR Kits (Sigma/Aldrich). For cDNA synthesis ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmid DNA was extracted from 100 mL cultures using a midi-prep kit (Millipore Sigma) and the DNA concentration was determined by NanoDrop spectrometer ND-1000.
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bioRxiv - Biochemistry 2023Quote: ... mtDNA was extracted from the resultant pellet using GenElute Mammalian Genomic DNA Miniprep Kit (Sigma). The remaining nDNA contamination was removed by treatment with Plasmid-Safe ATP-Dependent DNase (Lucigen ...
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bioRxiv - Physiology 2023Quote: ... and PCR was performed using KOD Xtreme™ HotStart DNA Polymerase Kit (71975-3, Novagen) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and eluted with purification buffer supplemented with 10 mM desthiobiotin (Sigma-Aldrich). The eluted fractions were concentrated by ultrafiltration using 30-kDa molecular weight cut-off Centricons centrifugal filter units (EMD Millipore ...
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bioRxiv - Biophysics 2021Quote: Routine chemicals used for protein expression and purifications were purchased from Sigma, Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... protein expression and purification and enzyme assays were obtained from Sigma-Aldrich, TCI chemicals ...
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bioRxiv - Developmental Biology 2022Quote: ... and purified by affinity purification using the aforementioned polypeptide (Sigma Aldrich Japan).
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bioRxiv - Biochemistry 2022Quote: ... All reagents used for protein purification and enzymatic assays were from Sigma.
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bioRxiv - Molecular Biology 2019Quote: ... Purification of rcCBP2 was performed by HIS-Select HF Nickel Affinity (Sigma) and gel permeation chromatography on Superdex 75 column (GE Healthcare Life Sciences) ...
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bioRxiv - Neuroscience 2020Quote: ... Sterile drinking water produced from a reverse osmosis water purification system (Millipore Rios with ELIX UV/Progard 2 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library purification and size selection was done using magnetic SpeedBeads™ (Sigma). We confirmed adaptor lengths through TapeStation High sensitivity T1000 (Agilent Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The HDR oligo was purchased from Sigma Aldrich (vendor purification by HPLC), and was additionally purified using ssDNA/RNA Clean & Concentrator kit (Zymo Research) ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by affinity purification on Amicon Ultra-0.5 Centrifugal Filter Devices (Millipore). IBTK phosphorylation was assessed using in vitro kinase assays ...
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bioRxiv - Bioengineering 2024Quote: Purification was done using Amicon 100k and 50k MWCO filters (UFC5100, Sigma). The manufacturer recommends that the filters be spun in the centrifuge at approximately a centrifugal force of 20k x g for 5 min ...
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bioRxiv - Genetics 2022Quote: ... Genomic DNA was extracted from pooled seedlings at the EBI laboratories using the GenElute Plant Genomic DNA Miniprep Kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA of the evolved mutants was isolated from LB overnight cultures using the GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich St. Louis, MO). The extracted DNA was evaluated by gel electrophoresis and quantified by a NanoDrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from frozen samples in the manual of the GenElute Bacterial DNA Isolation kit (Sigma Aldrich, NA2110-1KT, St. Louis, MO, USA) with the following minor modifications as previously described60 ...
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bioRxiv - Biophysics 2020Quote: ... Calf thymus DNA (CT-DNA, Sigma) was dissolved in the same cacodylate buffer ...
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bioRxiv - Cell Biology 2020Quote: Mouse genomic DNA was isolated from tail biopsies using a specific kit (Extract-N-Amp™ Tissue PCR Kit; Sigma-Aldrich, Darmstadt, Germany). PCR was performed using the primer pairs to amplify the DHX15 gene (primer forward ...
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bioRxiv - Plant Biology 2021Quote: ... was analyzed by extracting the gDNA with either the innuPREP Plant DNA Kit (Analytik Jena; Jena, Germany; T0) or the REDExtract-N-AMP plant Kit (Sigma Aldrich; T1, T2). The transgene was detected by amplifying the Ocs terminator of the hygromycin resistance cassette (Table S3).
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bioRxiv - Genetics 2021Quote: ... and Southern hybridization analyses (DIG DNA Labeling and Detection Kit, Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was subsequently digested using the DNase I Amplification Grade kit (Sigma Aldrich, Cat. No. AMPD1). We performed an RNA Integrity Number Analysis (RIN Analysis ...
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bioRxiv - Cell Biology 2022Quote: ... The amplified DNA was cleaned using a GenElute 96 Well PCR Clean-up kit (Sigma-Aldrich) and sent for sequencing.
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from 6 embryos using the Extract-N-Amp Tissue PCR Kit (Millipore-Sigma) and amplified using Amplitaq Gold Taq Polymerase (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from each fresh culture using GenElute Bacterial Genomic kits (Sigma-Aldrich, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: DNA was extracted using the GenElute™ Plant Genomic Miniprep Kit (Sigma-Aldrich, St. Louis, MO). DNA quality and quantification were determined using a Nanodrop spectrophotometer ND-1000 (Nanodrop Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Genomic DNA extracted from the whole embryos (using Extract-N-Amp™ Tissue PCR Kit, Sigma) was used for the genotyping after vasculature imaging.
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bioRxiv - Physiology 2022Quote: ... Total genomic DNA from blood was isolated using REDExtract-N-Amp Blood PCR Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Extraction method 1 used a modified protocol with the GenElute Bacterial genomic DNA kit (Sigma-Aldrich). The pellet was resuspended in 180 μl of kit-included lysis buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... DNA was extracted from fixed embryos after imaging using the Extract-N-Amp kit (Millipore-Sigma) in a total volume of 10 μl ...
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bioRxiv - Developmental Biology 2019Quote: ... DNA was extracted from fixed embryos after imaging using the Extract-N-Amp kit (Millipore-Sigma) in a total volume of 10 μl ...
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bioRxiv - Microbiology 2020Quote: ... Pop 9-12) (Figure 1) was isolated using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich). DNA-purity and - quantity was assessed using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... coli and Lactobacillus was isolated using the GeneElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich, USA) as described in the user manual ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from 6 embryos using the Extract-N-Amp Tissue PCR Kit (Millipore-Sigma) and amplified using Amplitaq Gold Taq Polymerase (ThermoFisher) ...