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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Contactin Associated Protein Like 3 CNTNAP3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cells were seeded at a density of 1 × 106 cells per well in 12-well plates and stimulated with LPS (100 ng/mL, M1-like - Sigma-Aldrich), IL4 (10 ng/mL ...
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bioRxiv - Bioengineering 2021Quote: ... Mature human white-like adipocytes were transfected in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522) to mimic the subcutaneous tissue environment ...
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bioRxiv - Neuroscience 2020Quote: ... SH-SY5Y cells were differentiated to a neurone-like phenotype prior to experimentation by incubation with 10 μM trans-retinoic acid (Sigma, UK) for 5 days [26].
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bioRxiv - Neuroscience 2021Quote: ... Three wells of BMEC-like cells per condition were then lysed in PBS containing 5% Triton X-100 (Sigma-Aldrich #T8787), and one well per condition was fixed in ice cold 100% methanol ...
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bioRxiv - Microbiology 2020Quote: For the measurement of proteasome activity a fluorometric assay kit (Proteasome 20S Activity Assay kit) that measures the chymotrypsin-like protease activity was used following manufactured instructions (Sigma-Aldrich). A total of 100 µg of protein extracts were incubated with LLVY-R110 substrate provided by the kit for 2 h at 30°C in dark ...
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bioRxiv - Microbiology 2019Quote: ... virus like particles were incubated with 20 μL of 10% SDS and 2 μL of proteinase K (Sigma, 20 mg/mL) for 20 min at 56 °C ...
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bioRxiv - Microbiology 2020Quote: ... Macrophage-like THP1 cells were pre-incubated with L-glutamic acid (5mM) or Sodium Pyruvate solution (2mM, Sigma catalog# S8636-100ml) for 2 hours before stimulating with Escherichia coli serotype O127:B8 LPS (50ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... virus like particles were incubated with 20 μL of 10% SDS and 2 μL of proteinase K (Sigma, 20 mg/mL) for 20 min at 56°C ...
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bioRxiv - Microbiology 2019Quote: Macrophage-like DH82 cells (ATCC CRL-10389) were grown in 25cm2 flasks with Eagle’s Minimum Essential Medium (EMEM) (Sigma, M0325, USA) with 10% heat inactivated FBS at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... THP-1 cells differentiation into mature macrophages-like state was induced through incubation with 10 ng/mL of phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) for 48 hours in a humidified atmosphere ...
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bioRxiv - Physiology 2021Quote: A drop of fresh culture containing control or spaghetti-like bacteria with 2 μM DiI dye (Sigma, CAS #41085-99-8) was placed on an NGM agar plate ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection for mature human white-like adipocytes was performed in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522). Both HeLa cells and mature human white like adipocytes were transfected with LNPs at a final mRNA concentration of 1.25μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... and slices were incubated overnight at 4 °C in a PBST-based antibody carrier solution containing 3% normal goat serum and rabbit anti-TPH2 primary antibodies (Millipore, ABN60) diluted 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by primary antibody incubation in 10% sheep serum at 4°C for 3 days (Anti-SCN5A antibody produced in rabbit (Sigma SAB2107930) 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... For visualization of blue protein bands from the prestained protein ladder the anti-BLUE antibody (Anti-BLUE, 2D2-F11, Millipore) was used (Schuchner et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were then mixed with the indicated primary antibodies and protein A/G agarose (Sigma, St. Louis, MO, USA) or anti-FLAG M2 agarose for overnight at 4 °C ...
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bioRxiv - Immunology 2019Quote: ... endogenous Asc from a total of 3.5 mg protein was immunoprecipitated with 1 μg of anti-Asc antibody and 60 μl of protein A/G agarose beads (Sigma), and bound Mul1 was detected with anti-Mul1 antibody by western blotting ...
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bioRxiv - Cell Biology 2019Quote: ... Gene Tex) antibody was incubated with protein G coated magnetic beads (PureProteome protein G magnetic beads, LSKMAGG10, EMD Millipore Corp.) for 1-3 hrs in IP (immunoprecipitation ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-HA or α-FLAG antibodies (Sigma-Aldrich, Cat # A8592).
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bioRxiv - Cell Biology 2023Quote: ... 20 μl of clear lysates and debris were loaded onto the same 10% Bis-Tris gel for the comparison of total protein (Coomassie staining) and ubiquitinated protein levels (anti-ubiquitin antibody, Sigma, 05-944 ...
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bioRxiv - Molecular Biology 2021Quote: ... We have then followed a traditional ChIP protocol in which we have enriched for the dCas9 bait and its associated complexes using an Anti-V5 Agarose Affinity Gel (Sigma). To further eliminate the background originating from the purification of non-chromatin associated bait complexes we have additionally added a histone enrichment step ...
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bioRxiv - Molecular Biology 2020Quote: ... Single-cell iAT2s were plated on Transwells at a density of 520,000 live cells/cm2 in 100ul of CK+DCI+Y (3uM CHIR99021, 10ng/ml KGF, 50nM dexamethasone, 0.1mM cAMP, 0.1mM IBMX, 10uM Rho-associated kinase inhibitor (Sigma Y-27632)) ...
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bioRxiv - Physiology 2023Quote: ... Oxygen levels were recorded for 3 min intervals at baseline (5 mM glutamate/2.5 mM malate), maximal capacity synthesis ATP (150 μM, ADP) and non-ATP-associated (1 μM Oligomycin, 75351, Sigma-Aldrich) conditions ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were immunoprecipitated with ACC antibody (CST) or ACC2 antibody (CST) and EZview Red Protein G Affinity Gel (Sigma).
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bioRxiv - Bioengineering 2021Quote: ... The unspecific antibody binding was blocked by suspending the tissue in 3% BSA (Sigma, Germany) in PBS for 1.5 hours in RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Moncolonal pan-RAS antibody (clone Ab-3; OP40-100UG; 1:1000) was obtained from Millipore. Rabbit polyclonal antibodies against SHP2 (sc-280 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed twice with PBS and anti-Gal-3 antibody (1:50; Sigma-Aldrich) was added ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunoprecipitation of Flag-tagged UNC-3 was performed using Flag antibody coated beads (Sigma, #A2220). For the IP ...
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bioRxiv - Molecular Biology 2019Quote: ... or anti-Strep mouse IgG monoclonal primary antibody (Sigma, 71590-3; 1:1000-1:2000) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies were diluted in DMEM high 3 glucose media containing 0.7% Low IgG BSA (Sigma), 1X Sodium Pyruvate ...
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bioRxiv - Microbiology 2020Quote: ... and probed with appropriate antibodies in PBS with 3% milk and 0.1% Tween20 (Sigma-Aldrich). Rabbit and mouse anti-SFP1 serum were used at a dilution of 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were probed using antibodies for ATG16L1 (MBL M150-3) and ACTB/actin (Sigma, A5441). Primary antibodies were detected using IRDye labelled secondary antibodies (LI-COR biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Coverslips were blocked with 3% BSA and incubated with primary antibodies (RPA - ab79398, H2AX - Millipore-05-636 ...
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bioRxiv - Physiology 2024Quote: ... Primary antibodies were used at described dilutions in 3% bovine serum albumin (BSA; Sigma-Aldrich) in Tris-buffered saline with Tween 20 and incubated overnight (4°C) ...
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bioRxiv - Microbiology 2024Quote: ... and co-IPs carried out using 3 μg monoclonal anti-FLAG M2 antibody (Sigma-Aldrich) or 30 μg polyclonal FlgV antibody or 30 μg polyclonal KhpB antibody and 20 μl of Pierce protein A/G magnetic beds ...
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bioRxiv - Bioengineering 2021Quote: ... 3 methylcholanthrene (3-MC; Sigma-Aldrich), contained in DMEM with a final concentration of 0.1% of dimethylsulfoxide (DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... 3-deazauridine (3-DU) (Sigma Aldrich) was used as a non-selective inhibitor of CTPS1 and CTPS2.
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the protein were pooled and concentrated using an Amicon with a 3 KDa cut-off (Millipore). The protein was conserved in 10 mM Tris-HCl pH7.63 and 33% glycerol ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant (total protein concentration ∼3 mg/ml) was incubated with ANTI-FLAG M2 agarose beads (Sigma Aldrich) at 4°C for 1 h with agitation ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using 3 kDa (KrACP, FabD) and 30 kDa (KrKSCLF) Amicon Ultra (Millipore, 15 mL capacity) molecular weight cutoff (MWCO ...
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bioRxiv - Biophysics 2021Quote: ... The protein solution was then concentrated using a 3 kDa cutoff centrifugal filter unit (Millipore, Burlington, MA, USA) and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated by 3–15% SDS-PAGE and transferred to polyvinylidene fluoride-FL (PVDF-FL, Millipore Sigma) membranes ...
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bioRxiv - Microbiology 2022Quote: ... stained with REVERT total protein stain (Licor Biosciences) and blocked with 3% bovine serum albumin (BSA) (Sigma-Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 6 mg RBD protein in 3 mL PBS were mixed with equal-volume Freund’s incomplete adjuvant (Sigma-Aldrich) for the first boosting ...
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bioRxiv - Neuroscience 2019Quote: ... Protein lysate was isolated in RIPA buffer supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich). Lysate (20 μg ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated to ∼500 μL using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore MWCO 3 kDa). The concentration of proteins was determined using JASCO V730 UV-vis spectrophotometer at 280 nm ...
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bioRxiv - Plant Biology 2022Quote: ... the same total protein extracts were incubated with Endoglycosidase H at 37 °C for 3 h (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were washed with TBST for 3 times and incubated for 1 h with a secondary antibody: anti-mouse IgG antibody (HRD) (Sigma-Aldrich, A9044) and anti-rabbit IgG antibody (HRD ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with primary antibody (15 µg/mL in 3% BSA in PBS): rabbit anti-KCNN4 antibody (IgG, clone HPA053841, Sigma Aldrich®) or control rabbit antibody (IgG ...
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bioRxiv - Molecular Biology 2019Quote: ... and COS-1 fibroblast-like cells derived from African green monkey kidney (ATCC, CRL-1650™) were maintained in 25 mM glucose DMEM (D6546; Sigma-Aldrich) supplemented with 10% fetal bovine serum ...