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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for Contactin Associated Protein Like 3 CNTNAP3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
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bioRxiv - Plant Biology 2023Quote: ... was used to detect proteins with horseradish peroxidase-conjugated anti-rabbit secondary antibodies (Sigma, A6154).
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bioRxiv - Pathology 2023Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were visualized with the FITC- and TRITC-conjugated secondary antibodies (Sigma, F6005, and T5393) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins reacting with primary antibodies were visualized by the Immobilon forte western HRP substrate (Millipore) for detecting peroxidase-conjugated secondary antibodies and analyzed with an ChemiDoc Touch (Bio-rad).
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bioRxiv - Microbiology 2023Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously [24 ...
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bioRxiv - Genetics 2023Quote: ... FLAG-tagged proteins were immunopurified using preformed complexes of anti-FLAG M2 antibody (Sigma Aldrich) and protein G Sepharose (GE healthcare).
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bioRxiv - Cell Biology 2022Quote: ... and the lysates were immunoprecipitated with specific antibody and protein A/G-Sepharose (Sigma-Aldrich) at 4 °C for 2 h ...
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bioRxiv - Plant Biology 2023Quote: ... antibody for the OsExo70F3 protein in pGBKT7 and with the anti-GAL4 activation domain (Sigma) antibody for AVR-Pii and ZiF effectors in pGADT7.
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bioRxiv - Neuroscience 2023Quote: ... We used primary antibodies for astrocytic marker glial fibrillary acidic protein (GFAP) (chicken, Millipore, AB5541). To quantify relative intensity of staining for GFAP we measured the average signal intensity in the region of interest and normalized it to that of the WT littermate mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were detected by Western blotting using the following primary antibodies against: Flag (M2, Sigma), ERK1/2 (3A7 ...
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bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...
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bioRxiv - Plant Biology 2023Quote: ... The AGO1 protein was detected by Western blot using a Flag-HRP antibody (A8592, Sigma).
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bioRxiv - Bioengineering 2023Quote: ... Antibodies against the following proteins were purchased from the indicated vendors: MAP2 (EMD Millipore AB5622); SLC17A6 (Alomone Labs AGC-036-GP) ...
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bioRxiv - Molecular Biology 2021Quote: ... 4% 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS, Sigma)] ...
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bioRxiv - Neuroscience 2022Quote: ... 3 3’-diaminobenzidine tetrahydrochloride hydrate (DAB) (Sigma SIG-32750) in sodium acetate ...
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bioRxiv - Bioengineering 2021Quote: ... 3- [(3- Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS, Sigma RES1300C), digitonin (Sigma D141) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 3-3’ diaminobenzidine tetrahydrochloride (DAB, Sigma Aldrich) staining ...
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bioRxiv - Microbiology 2022Quote: ... 3-amino-9-ethylcarbazol (3-AEC; Sigma Aldrich, Germany) was used as a chromogen ...
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bioRxiv - Cancer Biology 2019Quote: ... 14-3-3 Theta/Tau (Millipore Sigma T5942-.1MG). After exposure to the matching HRP-conjugated secondary antibody ...
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bioRxiv - Microbiology 2021Quote: ... 3-Amino-1,2,4-triazole (3-AT, A8056, Sigma Aldrich). Bovine liver catalase (C1345 ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µM CHIR99021/GSK-3 Inhibitor (Millipore Sigma 361571), and 1 µM PD0325901/MEK1/2 Inhibitor (Millipore Sigma 444968 ...
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bioRxiv - Microbiology 2021Quote: ... 3-(3-hydroxy-phenyl)propionate (Sigma Cat Number PH011597) and 3-hydroxycinnamic acid (CAS Number 14755-02-3) ...
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bioRxiv - Cancer Biology 2023Quote: ... Interleukin-3 (IL-3, Cat# SRP4135-10UG, Sigma, USA), Alexa Fluor 488 EGF complex (Cat# E13345 ...
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bioRxiv - Neuroscience 2023Quote: ... and activating transcription factor 3 (ATF-3, HPA001562, Millipore). After three washes with PBS ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/ml 3′,3′-diaminobenzidine tetrahydrochloride hydrate (Sigma), 24 units/ml catalase from bovine liver (Sigma) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 0.5 nM neurotrophin-3 (NT-3, Sigma #SRP6007). Cells were released by trituration through fire-polished glass pipettes of decreasing diameter ...
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bioRxiv - Neuroscience 2024Quote: ... 0.05% 3-3′ diaminobenzidine tetrahydrochloride hydrate (DAB, Sigma D5637) and 4% sucrose in PBS ...
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bioRxiv - Neuroscience 2020Quote: SH-SY5Y neuroblastoma cells were differentiated into mature neuron-like cells by treating with retinoic acid (4 µg/mL; Sigma-Aldrich, St. Louis, MO, #R2625) and BDNF (25 ng/mL ...
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bioRxiv - Microbiology 2022Quote: ... THP-1 monocytes were stimulated to differentiate into macrophage-like cells by treatment with 20 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich Co., St Louis, MO) for 18-20 h at 37°C and incubated further for 48 h to allow differentiation (Bhaskar et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... Extracts were then clarified at 4’000 × g for 10 min and concentrated to ~4 mg/mL total protein concentration using 3 kDa molecular weight cutoff (MWCO) Centriprep concentrators (EMD Millipore) to account for dilution during preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...
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bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were washed three times with the protein elution buffer (20 mM Tris-HCl, 150 mM NaCl, 10% glycerol) and eluted by adding 3× FLAG peptide (Sigma-Aldrich) in 60 µL of the protein elution buffer following the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was concentrated to 2-3 mg ml-1 using an Amicon ultracel-100 centrifugal filter unit (EMD Millipore) for amphipol exchange or nanodisc reconstitution ...
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Biochemistry 2022Quote: ... The final proteins were concentrated in a prewashed Amicon Ultra-15 Centrifugal filter (molecular weight cut off 3 kDa; EMD Millipore), flash-frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Biochemistry 2022Quote: ... Each recombinant protein was eluted twice in 250 μL EB containing 1 mM DTT and 250 μg/mL 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... the thiol groups of the above extracted protein samples from the culture supernatant of 3 groups were alkylated with 50 mM iodoacetamide (cat No. I6125, Sigma, USA) (30 min in dark at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... the column was washed with 20 column volumes of the lysis buffer and the protein was eluted with 3 mM desthiobiotin (Sigma, D1411).
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bioRxiv - Biochemistry 2022Quote: ... Cytidine 3′, 5′ cyclic monophosphate (3′, 5′ cCMP) and Cytidine 2′, 3′ cyclic monophosphate (2′, 3′ cCMP) were purchased from Sigma-Aldrich (Bangalore, India) and used without further purification ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblotting for specific proteins was carried out using the following primary antibodies: Mouse monoclonal antibodies against anti-FLAG M2 (Millipore Sigma, Billerica, MA), Jak1 (CST-50996) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851 ...
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bioRxiv - Genetics 2020Quote: ... Protein– antibody interaction was detected with either anti-mouse or anti-rabbit peroxidase-conjugated mouse IgG antibody (Sigma-Aldrich, St Louis, MO, USA), using Amersham™ ECL Plus western blotting detection system (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated with primary antibody Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit for detection of TAP-tagged proteins (Sigma-Aldrich, P1291-500UL) 1:1000 dilution in 5 % skimmed milk/PBST overnight at 4 °C followed by washing in 0.1%Tween/TBS ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... Coverslips were then incubated with 200μl of primary antibody solution for 1 h at RT and washed 3 times with PBS before incubation with secondary antibody solution containing 0,1μg/mL DAPI solution (Sigma) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Immunofluorescence staining was performed using antibodies against GFP (Servicebio, GB13227), CD31 (Servicebio, GB11063-3) and SPC (Millipore, AB3786).
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bioRxiv - Cell Biology 2022Quote: ... was washed with IP-lysis buffer and incubated with 3 µg of mouse anti-Flag antibody (Sigma, M2) for 1 h at 4°C ...