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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Coiled Coil Domain Containing 28B CCDC28B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Wild type and triple mutant human NRP1 b1b2 domain (residues 274-584) were expressed in Escherichia coli strain Rosetta-gami-2 (Novagen, Madison, WI) as a His-tag fusion in pET28b (Novagen) ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... an NPC1 domain C-targeting mAb were directly labeled with Duolink® In Situ Probemaker PLUS and Duolink® In Situ Probemaker MINUS (Sigma) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain) was cloned into pET-45b(+) vector (Novagen/Merck, Darmstadt, Germay) via ‘in vivo assembly’ (35 ...
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bioRxiv - Genomics 2023Quote: ... untethered Dam and Dam-Cbx1 constructs were fused to an ERT2 domain so that the fusion protein would be translocated to the nucleus upon 4-Hydroxytamoxifen addition (4-OHT, Sigma, Cat#SML1666) (Extended Data Table 1).
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in TBS-T containing 5% non-fat milk and probed against the primary antibodies: anti-PrP 8H4 (1: 10,000, Millipore), anti-PrP 3F4 (1 ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed five times with TBS containing 0.05% v/v Tween-20 and polyclonal anti-human antibody conjugated to HRP (Sigma) added for one hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary (1:100) and secondary (1:200) antibodies were diluted in PBS containing 1% (v/v) BSA (Sigma-Aldrich). Primary antibodies were incubated overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... diluted (1:2,000) in 5% skimmed milk in PBS containing 0.3% Tween-20 (PBST) and anti-α-tubulin monoclonal antibody (Sigma) (1:45,000 ...
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bioRxiv - Immunology 2022Quote: ... up to 5 × 107 cells were stained with monoclonal antibodies (Table 1) to cell surface proteins diluted in PBS containing 1 % BSA (Bovogen) and 0.1 % NaN3 (Sigma) (staining buffer ...
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bioRxiv - Microbiology 2022Quote: ... and then probed with 5 % skim milk (in 1x TBS-T) containing anti-GFP antibody (1:1,000; Sigma-Aldrich) overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... were coated with 3 μg of specific antibody or control IgG by resuspending them in 60 μl PBS containing 0.02% Tween-20 (P1379, Sigma), and rotated for 45 min at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... explants were incubated with selected primary antibodies diluted in blocking solution containing 20% serum in 0.3% Triton-X/PBS(Sigma) for 2 hrs at room temperature (RT) ...
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bioRxiv - Physiology 2021Quote: ... 1 mM EDTA in Dulbecco’s Phosphate Buffered Saline (DPBS) pH 7.4) (120) containing an antibody against PCM1 (1:1000, HPA023370, Sigma Aldrich). Samples were incubated using a tumbler (40 rpm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Mice were intravenously injected with an antibody mixture in sterile 0.9% sodium chloride containing TRITC/bovine serum albumin (Sigma; to label the vasculature ...
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bioRxiv - Neuroscience 2022Quote: ... The brain slices containing HVC were collected and incubated overnight with a rabbit anti-GFP antibody (EMD Millipore, AB3080P) in 1xPBS containing 10% donkey serum and 0.2% Triton at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... The filters were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween (TBS-Tween) and then incubated overnight with antibodies directed against specific proteins: AMPK (07-181, Millipore), p-AMPK (#2535 ...
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bioRxiv - Biochemistry 2023Quote: ... Immunolabeling of Flag-tag was done overnight in 4 °C in the above mentioned buffer containing a 1:5,000 dilution of a monoclonal anti-Flag horseradish peroxidase conjugated antibody (Sigma). Membranes were washed 3×10 minutes with PBS ...
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bioRxiv - Bioengineering 2023Quote: Jurkat cells were cultured in cell culture medium containing 1.7 μg/ml anti-TCR antibody (Sigma-Aldrich, 05-919) overnight followed by anti-CD69 antibody staining (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was probed with an α-FLAG antibody (Sigma; 1:1,000 in 1x TBS-T containing 3% BSA) at 4 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in a primary antibody solution containing 1:250 mouse anti-NeuN (Millipore Sigma; MA, USA) or 1:200 goat anti-DCX (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... corresponding to residues within the LCC domain) were used at 50 µg mL-1 (clone CL3112, AMAB91123, Sigma-Aldrich, St. Louis, Missouri, US) and mouse IgG for IHC (31903 ...
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bioRxiv - Plant Biology 2022Quote: ... Various recombinant TFIIIA-7ZF proteins with an intein-chitin binding domain (CBD) tag were overexpressed using the Escherichia coli BL21(DE3) Rosetta strain (EMD Millipore, Burlington, MA). For each construct ...
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bioRxiv - Neuroscience 2023Quote: To label AMPA receptors in the plasma membrane the N-terminal extracellular domain of GluA2 was stained with monoclonal mouse anti-GluA2 (Millipore, # MAB397, 1:500) for 2 hours without prior permeabilization ...
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bioRxiv - Molecular Biology 2019Quote: ... Mixture of experimental chromatin and spike- in chromatin was then incubated with a mix containing 4 µg of H3K4me3 antibody (CS200580; Millipore) and 2 µg of spike-in antibody (104597 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections containing SN and VTA were incubated with antibodies directed against tyrosine hydroxylase (mouse monoclonal, 1:1000, Merck-Millipore MAB318) and mCherry (rabbit polyclonal ...
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bioRxiv - Microbiology 2021Quote: ... overnight cultures of CA-MRSA strain USA300 grown in MHB medium were diluted to OD600 0.01 with fresh MHB medium containing 1 μM of antibodies and 4 μg/ml nisin (Sigma-Aldrich) or PBS as a control in 96 well plates ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-tyrosinase (T311, Novocastra) primary antibodies diluted in phosphate buffered saline containing 1% bovine serum albumin (PBS-BSA 1%; BSA, Sigma).
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bioRxiv - Animal Behavior and Cognition 2022Quote: sections were incubated in a solution containing a rabbit anti-orexin A antibody (1:1000; AB3704, Millipore, Temecula, CA, USA) for two days followed by incubation in a solution containing Alexa-Fluor Plus 594 donkey anti-rabbit antibody (1:3000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The primary antibody was then replaced with blocking solution containing 5 μg/mL bisBenzimide H 33342 trihydrochloride (Sigma-Aldrich, B2261) and goat anti-mouse Alexa Fluor 488 secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... membranes were incubated with the indicated primary antibody in either PBST containing 5% skim milk or 5% (w/v) BSA (Fraction V, Sigma) for at least 1h at RT or overnight at 4°C ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated overnight at room temperature in blocking buffer containing primary antibody (mouse anti-UBE3A (Sigma SAB1404508 clone 3E5)) at 1:250) ...
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bioRxiv - Neuroscience 2020Quote: ... All primary and secondary antibodies were diluted in TBST containing 5% bovine serum albumin (BSA, Sigma Aldrich, St. Louis, MO). Membranes were then developed with chemiluminescent substrate (SuperSignal West Dura ...
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bioRxiv - Molecular Biology 2021Quote: ... cells expressing GIPR and GLP-1R were incubated with 1:300 anti-Flag primary antibody (diluted with PBS containing 5% BSA, Sigma), and those expressing GCGR were incubated with 1:50 anti-GCGR antibody (diluted with PBS containing 5% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight at 4°C in blocking solution containing one of the following primary antibodies: anti-GFAP (1:2000; Merck Millipore) and anti-Iba1 (1:500 ...
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bioRxiv - Physiology 2021Quote: ... Muscles were then incubated in blocking solution containing rabbit anti-heavy NF200 primary antibody (N4142 Sigma, Germany; 1:200 dilution) and anti-synaptophysin antibody (1:25 dilution as above) ...
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bioRxiv - Developmental Biology 2021Quote: ... the antibody (Table S4) was added to the cell suspension resuspended in a buffer containing 10% BSA (Sigma Aldrich, #A8022) and 0.5M EDTA (ThermoFisher Scientific #15575020) ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were transferred to a solution containing anti-Vesicular Glutamate Transporter 2 (VGlut2) polyclonal guinea-pig antibody (AB2251-I Sigma Millipore) in a 1:2000 dilution and kept overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... 1×106 yeast cells were incubated in staining buffer (phosphate buffered saline + 0.5% BSA + 2mM EDTA) containing anti-Flag fluorescein isothiocyanate antibody (2 μg/mL; clone M2, Sigma-Aldrich) and the probes for 30 minutes at RT in darkness prior to washing 2 times in ice cold staining buffer ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:1000 in phosphate-buffered saline (PBS) containing 0.05% Tween 20 (PBS-T) followed by anti-rabbit horseradish peroxidase (HRP)-coupled antibody (Sigma-Aldrich) diluted 1:1000 in PBS-T ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Immunology 2020Quote: ... TZM-bl cells (1 X 104) were added into the mixture virus-antibody mixture containing 25 μg/ml DEAE-dextran (Sigma). The plates were further incubated for 48 h and the extent of virus neutralization was assessed by measuring relative luminescence units in a luminometer (Victor X2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in blocking buffer containing 0.2% Tween-20: Puromycin (1:10000, Millipore #MABE343), APOE (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed three times with PBS + 0.05% Tween-20 and then incubated with PBS + 1% milk solution containing a 1:10.000 anti-rabbit horseradish peroxidase conjugated secondary antibody (A0545, Sigma-Aldrich). The membrane was washed three times with PBS + 0.05% Tween-20 and then incubated for 5 min with Pierce ECL Plus substrate (Thermo Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were then incubated overnight at 4 °C with 10 ml TBS-T containing either anti-c-myc-peroxidase antibody (1:10,000; Sigma, A5598), or anti-HA primary antibody (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with 0.05M PB containing 0.3% triton X-100 and the primary antibody (rabbit anti-FG, AB153-I, 1:10000, Sigma-Aldrich) at 4°Covernight ...
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bioRxiv - Molecular Biology 2023Quote: ... BSA for 15 min at room temperature before incubating with primary anti-Flag antibody (diluted with PBS containing 5% BSA at a ratio of 1:300, Sigma) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK (1:1000, Sigma Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... Nonspecific antibody binding was blocked by incubation with Tris buffered saline + 0.05% Tween-20 (TBST) containing 10% dry milk (Sigma Aldrich) for one hour at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were first stained for 20 min at RT with extracellular antibodies in the staining mix containing FACS buffer (dPBS (Sigma) with 2% HI FBS (Gibco ...
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bioRxiv - Zoology 2023Quote: ... We stained animals with primary and secondary antibodies for 5 days at a 1:800 dilution in PBST containing 1% BSA (Sigma). After washing in PBST ...