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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Coiled Coil Domain Containing 28B CCDC28B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The pET-28b (Novagen) derivative ...
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bioRxiv - Microbiology 2020Quote: The vector pET-28b(+) (Novagen) was used for the expression of PvdS ...
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bioRxiv - Biochemistry 2024Quote: ... coil Rosetta (DE3) pLysS (Novagen), and cells were grown in LB media until the cell density reached an OD600=0.6 ...
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bioRxiv - Microbiology 2020Quote: ... pET-28b vector (Novagen-EMD Millipore), ROSETTA 2 (DE3 ...
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bioRxiv - Microbiology 2020Quote: ... pET-28b vector (Novagen-EMD Millipore), ROSETTA 2 (DE3 ...
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bioRxiv - Molecular Biology 2024Quote: pET-28b(+) expression vector (Novagen, Germany) was used for cloning of complete ORF of SlHY5 ...
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bioRxiv - Immunology 2023Quote: ... and ligated into pET-28b vector (Novagen) or a modified pET-28a vector (Novagen ...
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bioRxiv - Biophysics 2021Quote: ... coli expression plasmid vector pET-28b (Novagen, MA) was used to construct all the gp17 WT and mutant clones (37) ...
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bioRxiv - Neuroscience 2020Quote: ... was inserted into the pET-28b(+) vector (Novagen). At the N-terminus of GAPDH ...
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bioRxiv - Biochemistry 2023Quote: ... into the same sites of pET-28b(+) (Novagen); thereby the recombinant proteins include either a C-terminal or an N-terminal 6×His-tag ...
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bioRxiv - Microbiology 2020Quote: ... was encoded in a pET-28b vector (Novagen – EMD Millipore), which also included a thrombin protease cleavage site (His6-Thrombin Site-Tsg101 ...
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bioRxiv - Microbiology 2020Quote: ... was encoded in a pET-28b vector (Novagen – EMD Millipore), which also included a thrombin protease cleavage site (His6-Thrombin Site-Tsg101 ...
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bioRxiv - Microbiology 2020Quote: ... These PCR products were cloned into pET-28b(+) (Novagen, USA) to construct tagged Fnr proteins with His-tag at the N-terminus of Fnr1 and C-terminus of Fnr3 respectively ...
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bioRxiv - Biochemistry 2024Quote: ... were used for the transformation of pET-28b(+) plasmids (Novagen). Phenylmethylsulfonyl fluoride protease inhibitor (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2024Quote: ... Two coiled silver wires (0.5 mm in diameter, Cat# 265586, Sigma-Aldrich, USA) were placed on the corneal surface of the eyes ...
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bioRxiv - Molecular Biology 2023Quote: ... The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T). The intensity of the signal from retained anti-AGO1-N-coil antibody was quantified using Image J and fitted using Nonlinear Least Square (NLS ...
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bioRxiv - Molecular Biology 2023Quote: ... and re-cloned into NcoI/XhoI sites of the pET-28b(+) vector (Novagen) using sequence and ligation-independent cloning (SLIC ...
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bioRxiv - Plant Biology 2019Quote: ... for the HMA domains in pGBKT7 and anti-GAL4 activation domain (Sigma) antibodies for the AVR-Pik effectors in pGADT7.
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bioRxiv - Plant Biology 2020Quote: ... for the HMA domains in pGBKT7 and anti-GAL4 activation domain (Sigma) antibodies for the AVR-Pik effectors in pGADT7.
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bioRxiv - Developmental Biology 2022Quote: ... Immunohistochemistry was performed using antibodies recognizing EF-Hand domain family D1 (HPA056959; RRID:AB_2683288; Sigma Aldrich), and type XV collagen (HPA017915 ...
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bioRxiv - Plant Biology 2023Quote: ... antibody for the OsExo70F3 protein in pGBKT7 and with the anti-GAL4 activation domain (Sigma) antibody for AVR-Pii and ZiF effectors in pGADT7.
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bioRxiv - Molecular Biology 2023Quote: ... or anti-AGO1-N-coil (1:1000 in 5% milk in PBS-T) followed by anti-Rabbit antibody (1:10,000 [Sigma] in 5% milk in PBS-T). The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T) ...
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bioRxiv - Plant Biology 2023Quote: The complete open reading frame of NtHY5 was amplified and cloned into pET-28b(+) vector (Novagen, Germany). The construct was transformed into E ...
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bioRxiv - Molecular Biology 2020Quote: 10 μL samples containing 10 μM protein (TASOR variants or PARP1 catalytic domain) in the presence or absence of benzamide (Sigma) were loaded into glass capillaries (Nanotemper ...
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bioRxiv - Molecular Biology 2024Quote: ... For shRNA-mediated knockdown, all TRC pLKO.1 vectors (targeting the YTH domain-containing proteins, THOC1, and THOC2) were purchased from Sigma. The TRIPZ doxycycline (Dox)-inducible lentiviral YTHDC1 shRNA vector is purchased from Dharmacon.
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bioRxiv - Cell Biology 2021Quote: ... BamHI- and HindIII-digested FUBI or ubiquitin PCR fragments were cloned into a modified pET-28b(+) vector (Novagen) allowing for expression of a His6-tagged fusion protein with EGFP that was cloned into the HindIII and XhoI sites ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal rat E-cadherin antibody that specifically binds to the extracellular domain of E-cadherin (Sigma, U3254) was used at 1:1600 dilution ...
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bioRxiv - Biophysics 2021Quote: ... and the flow-through fractions containing the MgtE TM domain protein were concentrated using an Amicon Ultra 50 K filter (Merck Millipore, USA). After concentration ...
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bioRxiv - Microbiology 2021Quote: ... The NdeI and EcoRI-excised insert of pUCIDT_atsJ was ligated into the same restriction sites of pET-28b (Novagen) and the resulting plasmid was introduced into E ...
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bioRxiv - Microbiology 2020Quote: ... Bound domain C was then detected using an HRP-conjugated anti-FLAG antibody (Sigma-Aldrich, St. Louis, MO) and Ultra-TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... cells were incubated with the primary antibody (anti-Htt raised against the Proline-rich domain (PRD) (MAB5492, Millipore) at a dilution of 1/500 in PBST for 2 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... N-SH2 domain (ABS233) was obtained from Millipore Corp ...
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bioRxiv - Cell Biology 2024Quote: ... FH2 domain inhibitor SMIFH2 (2µM; Sigma-Aldrich #344092); Rac activation ...
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bioRxiv - Neuroscience 2023Quote: ... containing primary antibodies (arrestin3, Sigma-Aldrich, HPA063129 ...
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bioRxiv - Molecular Biology 2020Quote: ... the gene encoding residues Gly63–Leu231 were cloned into the NdeI and EcoRI sites of the pET-28b vector (Novagen - Merck Millipore). Escherichia coli BL21-CodonPlus(DE3 ...
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bioRxiv - Microbiology 2022Quote: ... the soluble portion of these proteins (excluding the leader peptide and the predicted α1N helix) was fused to non-cleavable N-terminal 6His or Strep II tags and cloned into pET-28b (Novagen). We amplified pilA directly from the S ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-Gal4 activation domain (1:1000, Millipore Sigma). Alexa Fluor secondary antibodies were used (1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... MBP domain was cleaved with TEV protease (Sigma Aldrich). These proteins were flash-frozen with 40% glycerol in a solution containing 50 mM Sodium Phosphate (pH 7.2) ...
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bioRxiv - Developmental Biology 2020Quote: ... Septa in vegetative hyphae and ascogonial coils were stained using Calcofluor White M2R (CFW; Sigma [Sigma Chemical], St. Louis, MO) with a concentration of 1 μg/ml CFW stock solution diluted 1:400 in A ...
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bioRxiv - Developmental Biology 2020Quote: ... Septa in vegetative hyphae and ascogonial coils were stained using Calcofluor White M2R (CFW; Sigma [Sigma Chemical], St. Louis, MO) with a concentration of 1 μg/ml CFW stock solution diluted 1:400 in A ...
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bioRxiv - Neuroscience 2022Quote: ... we added polyclonal antibodies against the extracellular domain of TrkB (1:50 rabbit anti-TrkB, Millipore, cat no. AB9872, RRID:AB_2236301) together with 20 ng/mL BDNF for 2.5 hours ...
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bioRxiv - Biophysics 2020Quote: ... and 10 μg/ml anti-E-cadherin blocking antibody that targeted the extracellular domain of E-cadherin (Gumbiner, Stevenson and Grimaldi, 1988) (uvomorulin, monoclonal anti-E-cadherin antibody, Sigma); after incubation ...
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bioRxiv - Molecular Biology 2020Quote: ... the gene encoding residues Gly63–Leu231 were cloned into the NdeI and EcoRI sites of the pET-28b vector (Novagen - Merck Millipore). Escherichia coli BL21-CodonPlus(DE3 ...
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bioRxiv - Biochemistry 2022Quote: SPOC domains were expressed in E.coli Rosetta2 (DE3) cells (Novagen). O/N cultures were diluted 1:50 in 2xTY ...
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bioRxiv - Microbiology 2022Quote: ... 5 μM caveolin-scaffolding domain peptide (Sigma Aldrich Inc; 219482), 5 μg/mL filipin III (Thermofisher ...
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bioRxiv - Plant Biology 2021Quote: ... for the HMA domains in pGBKT7 and anti-GAL4-AD (Sigma) antibodies for the APikL effectors in pGADT7.
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bioRxiv - Microbiology 2020Quote: ... N-terminal domain of SadP was labelled with FITC (Sigma-Aldrich) in 0.2 M borate buffer ...
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bioRxiv - Plant Biology 2023Quote: ... The membranes were probed with anti-GAL4 DNA Binding domain (Sigma) antibody for the OsExo70F3 protein in pGBKT7 and with the anti-GAL4 activation domain (Sigma ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used for immunofluorescence on whole-mounted retinal sections were brain-specific homeobox/POU domain protein 3A (Brn3a; Millipore; cat #MAB1585; RRID:AB_94166) at 1:1000 and GFAP (1:1000) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight while shaking at 4°C in the blocking solution that contained combinations of the following primary antibodies: mouse anti- three microtubule-binding domain tau protein (3R-tau, 1:500, Millipore, Cat# 05-803), rabbit anti-purkinje cell protein 4 (PCP4 ...