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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for CTLA 4 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Chemicals used: 4-Aminopyridine (4-AP, Sigma #A78403), tetrodotoxin (TTX ...
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bioRxiv - Developmental Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT, Cat#: H7904, Sigma-Aldrich) was dissolved at 10mg/ml in peanut oil and 1mg was administered by intraperitoneal injection from E10.5 for 4 consecutive days ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT; 1 μM; #H7904 Sigma) or vehicle was added to the cell culture medium to activate Cre-LoxP recombination ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM MgCl2 and 4% DMSO (Sigma, 276855). The mixture was incubated for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 3-ethoxy-4-hydroxybenzaldehyde (121-32-4, Sigma), N-(acid-PEG10)-N-bis(PEG10-azide ...
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bioRxiv - Neuroscience 2024Quote: ... and 4-aminopyridine (4-AP, 100 μM, Sigma), mecamylamine (10 μM ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged SMOCtail and IFT38 protein fusions were expressed in Rosetta2(DE3)-pLysS cells grown in 2xYT medium (Millipore Sigma, Y2627) at 37°C until the optical density (OD ...
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bioRxiv - Molecular Biology 2021Quote: ... After washing Laemmli buffer 1X was added followed by boiling and loading on SDS-PAGE for visualization by western blotting using anti-His (Clonetech 631212) and anti-GST (Sigma G1160) antibodies.
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bioRxiv - Cell Biology 2019Quote: ... fragments of NUP153 (AAS 228-611) were fused C-terminally to GST in pGEX4T-3 and expressed in BL21 DE3 cells (Sigma CMC0014). Cell pellets were lysed (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... After washing 3 times with PBS protein was eluted from beads in Laemmli buffer and then used for protein blot with anti-GST and anti-T7 (EMD Millipore). For PR2 detection in S1 Fig ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with 500 μg Flag-GST-T6B peptide [46] and 80 μl of anti-Flag M2 Magnetic beads (Sigma, #M8823) for at least 3 hours rotating at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2020Quote: ... The protein was separated on a 10% SDS-PAGE gel and further analyzed by immunoblotting using anti-His and anti-GST antibody (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... and Ago1-4 were pulled down using 500 μg of Flag-GST-T6B peptide (50) and with 80 μl anti-Flag M2 Magnetic beads (Sigma #M8823) for 3 hrs on a rotor at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... was loaded alongside the pulldown samples which were resolved on 10% SDS polyacrylamide gels and subject to western blotting using anti-GST antibody (Sigma G7781). Membranes were developed with an ECL plus Chemiluminescent kit (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... 7μL of each dilution was then used in SDS-PAGE alongside a dilution series of recombinant GST protein (Sigma-Aldrich #SRP5348) from 5ng-200ng ...
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bioRxiv - Cell Biology 2023Quote: ... MBP- and GST-tagged proteins were purified by gel filtration on a Superdex 200 column (Sigma-Aldrich Cat# GE17-5175-01) and concentrated using a 30K Amicon filter.
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bioRxiv - Molecular Biology 2024Quote: ... Mouse monoclonal anti-Flag (M2), mouse monoclonal anti-Actin (MAB1501, clone C4) and rabbit polyclonal anti-GST (G7781) were from Sigma-Aldrich. Rabbit polyclonal anti-FOXK1 (MNF ...
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bioRxiv - Microbiology 2020Quote: ... Human serum-supplemented medium was made through addition of complete human serum (Sigma Aldrich) into CDM to a final concentration of 5% (v/v) ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in an equal volume of human serum from human male AB plasma (Sigma) and incubated for 16 h at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 10 % heat-inactivated human serum from human male AB plasma (Sigma-Aldrich), 2.5 μM zoledronic acid (Sigma-Aldrich ...
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bioRxiv - Genetics 2019Quote: ... from HEK293 cells stably expressing p3XFLAG-ADCK4 or -BAP were incubated with 80 μL of FLAG M2 agarose beads (Sigma-Aldrich) for 48 h at 4°C in an orbital shaker ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Cell Biology 2021Quote: ... GBM#U3013 cells were infected with lentiviral particles generated by transfecting HEK293 cells with pLKO.1-puro plasmids from the Mission shRNA library (Sigma-Aldrich). Briefly ...
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bioRxiv - Immunology 2021Quote: ... HEK293 and HEK293T cells (gifts from Caetano Reis e Sousa, The Francis Crick Institute, UK) were maintained in DMEM (Sigma-Aldrich) containing 4.5 g/L glucose ...
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bioRxiv - Immunology 2022Quote: Luciferase-expressing HeLa or HEK293 cells were seeded into a U-bottomed 96-well plate plus D-Luciferin (75 μg/ml; Sigma-Aldrich) for cytotoxicity assessment ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were cultured in serum-free and phenol red-free media containing 4.5 g/L glucose (Sigma-Aldrich, Dorset, UK), 1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2019Quote: Cell lysate from HEK293 transfected with FLAG-Podocin (WT, R45A, R50 and R54A) was immobilized on anti-FLAG beads (Sigma-Aldrich). The beads were washed with IP buffer (1%–2% Triton-X ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Cancer Biology 2023Quote: ... Miapaca2, Hek293 were cultured with high-Glucose DMEM medium (Fisher, SH30423FS) supplemented with 10% FBS and 1% Pen-Strep (Sigma, 11074440001). The BxPC3 cell were cultured with RPMI1640 (Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... To pcw-KRAS-G12D-blast vector was packaged into a viral assembly by co-transfecting with PMD2.G (addgene, 12259) and PSPAX2 (addgene,12260) into HEK293 T cells using polyethyleneimine (PEI) transfection reagents (Sigma 408727). The virus-containing medium was used to infect HPNE E6/E7/st40 cells with 8ug/ml polybrene ...
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bioRxiv - Cell Biology 2020Quote: ... and human chorionic gonadotropin (Sigma), 48 hours apart ...
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bioRxiv - Biochemistry 2020Quote: ... human cathepsin S (Millipore Sigma), bovine spleen cathepsin C (Millipore Sigma) ...
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bioRxiv - Biophysics 2022Quote: ... 20nM human insulin (Sigma-Aldrich), 2 μM rosiglitazone (Cayman) ...
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bioRxiv - Cancer Biology 2022Quote: ... 5% human serum (Sigma-Aldrich), 1x MEM Vitamins (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... 5% human serum (Sigma-Aldrich) and 100 μg/ml primocin (Invivogen) ...
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bioRxiv - Cell Biology 2022Quote: ... 5% human serum AB (Sigma), penicillin-streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: ... Human Nuclei (1:300, Millipore), Human GFAP (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... Human CTSD ELISA (Millipore #1317) was performed on the culture media according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... and human IFN-γ (Millipore) were used for stimulation of cells ...
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bioRxiv - Bioengineering 2020Quote: ... or human plasma FN (Sigma) solutions ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... and human Fn (Sigma-Aldrich) as described previously (Bao et al. ...
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bioRxiv - Immunology 2021Quote: ... A human IgG (Sigma #I2511) or IgM (Sigma #I8260-1MG ...
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bioRxiv - Bioengineering 2021Quote: ... Human serum albumin (Sigma, USA) was used as a control ...
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bioRxiv - Bioengineering 2021Quote: ... human insulin (Sigma-Aldrich, I2643), single strand DNA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... hemoglobin (human, Millipore Sigma #G4386), gamma-globulin (human blood ...
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bioRxiv - Systems Biology 2021Quote: ... 0.02% human-EGF (Sigma, E5036), and 0.01% cholera toxin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... human plasma-derived apoE (Sigma), or DMSO (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... against human Sept9 (Sigma-Aldrich, Cat#HPA042564 and HPA050627 ...
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bioRxiv - Cell Biology 2021Quote: ... C5 deficient serum human (Sigma); Phalloidin-Alexa 488 & 647 (Molecular Probes) ...