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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for CTLA 4 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... 4 × 10-4 M monothioglycerol (Sigma-Aldrich), 150 μg/mL transferrin (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... 4-hydroxytamoxifen (4-OHT) (H7904, Sigma-Aldrich) was added to cells at 1 μM working concentration for the indicated time.
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bioRxiv - Neuroscience 2024Quote: ... 4-aminopyridine (4-AP, 100 μM; Sigma) and tetrodotoxin (TTX ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant insulin human (≥ 98%), somatostatin-14 (≥ 97%, human HPLC grade), and urocortin-3 (≥ 97%, human HPLC grade) were all purchased from Sigma-Aldrich. Taxonomy will only be indicated for insulin ...
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bioRxiv - Molecular Biology 2020Quote: ... and GST-10xCTD repeats were expressed in BL21 Star (DE3) cells grown in Overnight Express Instant TB Medium (EMD Millipore) at 37°C and induced at an OD600 of 1.0 by decreasing the temperature to 18°C and growing for an additional 16 hours ...
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bioRxiv - Molecular Biology 2020Quote: Aliquots of 3 μg of GST-LC3B (Viva Bioscience) were incubated alone or with 250 ng of His-STK4 (Millipore) in in vitro kinase reaction buffer (10 mM MgCl2 ...
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bioRxiv - Microbiology 2021Quote: ... The primary immunization was carried out using 450 μg of GST cleaved PfCD protein and an equal volume of Freund’s complete adjuvant (F5881 # Sigma-Aldrich) via the intraperitoneal route ...
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bioRxiv - Immunology 2020Quote: ... 1mM EDTA, and protease inhibitors (aprotinin, leupeptin, PMSF) GST-Hhex fusion proteins were purified over glutathione-Sepharose 4B beads (Sigma) and eluted with 10 mM glutathione according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: A peptide corresponding to residues 1 – 159 of HyYap was expressed as GST-fusions using the GEX4t-1 vector (Millipore) in E ...
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bioRxiv - Developmental Biology 2019Quote: Peptides corresponding to the C-terminal portions of HyFat (residues 4191 – 4393) and HyDs (residues 9144 - 9799) were expressed as GST-fusions using the GEX4t-1 vector (Millipore) in E ...
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bioRxiv - Cancer Biology 2019Quote: ... Activated p38MAPKα was added to a final concentration of 60 pM for reactions with 1nM non-phosphorylated GST-MK2 (Millipore Corporation ...
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bioRxiv - Biochemistry 2020Quote: ... phosphorylation of peptides by the active Pro-directed kinase GST-CDK2/Cyclin A2 (100 ng purified from Sf9 cells, Sigma) was also confirmed in the presence or absence of the ATP competitive inhibitor ...
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bioRxiv - Systems Biology 2020Quote: ... the cDNA was inserted in frame with a 6xHis-GST TEV-cleavable tag into a modified version of pET-30b (Novagen). The NCK2 SH3 shuffled chimeras were constructed using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant GST fusion proteins were eluted by resuspending the resin in elution buffer containing 15 mg/mL reduced L-glutathione (Sigma) and incubated at 4°C for 4 hours ...
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bioRxiv - Plant Biology 2022Quote: ... A 1–month–old ICR mouse (SLAC laboratory animal) was injected with 50 μg GST–CAR9 protein emulsified with Complete Freund’s adjuvant (F5881, Sigma–Aldrich). Two weeks later ...
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bioRxiv - Cell Biology 2019Quote: ... and His12-Arf1 proteins were expressed as the N-terminal GST-His6-tagged form in Escherichia coli BL21(DE3) cells (Novagen) harboring pET-41-based vectors in Lysogeny Broth (LB ...
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bioRxiv - Plant Biology 2021Quote: ... GSTU5 was detected by the anti-GST primary antibody (Novex, 1:500) and anti-mouse secondary antibody (Sigma, 1:5000).
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bioRxiv - Cell Biology 2019Quote: ... Elution was done by incubation of the beads in 30µl GST elution buffer [50 mM Tris-HCl (pH 8.0) and 20mM reduced glutathione (Sigma-Aldrich, #G4251)] at room temperature for 20mins ...
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bioRxiv - Biochemistry 2021Quote: ... FH-nsp13 and GST-nsp13 were affinity purified from insect cell lysates using anti-FLAG M2 Affinity Gel (Sigma-Aldrich) or Glutathione Sepharose 4 Fast Flow resin (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: A Rab11/Ypt32 construct harbouring a GST tag at the N-terminus was expressed recombinantly in Rosetta2 cells (EMD Millipore) and purified using the procedure described previously (21) ...
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bioRxiv - Molecular Biology 2022Quote: ... GST-Cter-α4 or GST-β3) was mixed with 1.5 μL of active Ccnd1-Cdk4 complex purified from baculovirus (Sigma C0620), 10 mM ATP ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Immunology 2021Quote: HEK293 and transfected derivatives were left unstimulated or stimulated for 24h with 10 ng/mL lipopolysaccharide (LPS) from Salmonella (Sigma), 10 ug/mL isolated S protein ...
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bioRxiv - Neuroscience 2020Quote: ... the HEK293 and primary neuronal cells were plated on the 12mm #1 coverslips coated with poly-D-lysine (Sigma-Aldrich). For the 96-well plate screening assays ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were seeded at a low density on 0.01% (w/v) poly-L-lysine (Sigma, St Louis, MO, USA) coated coverslips and were transfected the subsequent day using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The exogenous expression of ACE2 and TMPRSS2 in the HEK293-C34 cells was induced by addition of doxycycline hydrochloride (1 μg/ml) (Sigma). TMPRSS2-expressing Vero E6 (VeroE6/TMPRSS2 ...
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bioRxiv - Molecular Biology 2020Quote: pCDNA3 vectors expressing HA-tagged full size LARP6 or La-domain were transfected into HEK293 cells and 24h after transfection the cells were fixed and incubated with anti-HA primary antibody (Sigma), followed by incubation with fluorescent secondary antibody ...
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bioRxiv - Immunology 2019Quote: ... Vectors were transiently transfected in HEK293-6E cells and antibodies were purified from supernatant using Robocolumn Eshmuno A (EMD Millipore) or Monofinity A Resin prepacked columns (Genscript) ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected with plasmids encoding different ACE2 orthologs (Table S1) by polyetherimide (PEI) (Sigma, St Louis, MO, USA). After 40 hrs incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... thereafter named HEK293) were cultured under 5% CO2 at 37°C in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Sigma–Aldrich, D6046) supplemented with 10% foetal bovine serum (FBS-Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Then pSIREN-RetroQ-Sirt7 and the negative control vector (pSIREN-RetroQ) were transfected into HEK293 cells using PEI transfection reagent (Sigma). The pSIREN-RetroQ-Sirt7 and the negative control vector (pSIREN-RetroQ ...
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bioRxiv - Biochemistry 2023Quote: HEK293 Flp-In T-Rex cells were cultured in DMEM high glucose medium supplemented with 10% FBS (Sigma-Aldrich F0804), 2mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: PFKFB3 depletion was carried out in HEK293 cells using siRNAs (80 nM; transfected twice on consecutive days) from SIGMA (SASI_Hs01_00065120). The cells were treated with control or specific siRNA and lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: 2×10^5 Vero E6 cells or HEK293-ACE-2 were cultured in 24-well plates in Dulbecco’s Modified Eagle medium (DMEM, Sigma D5796) containing 10% of fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... and GIGYF2-KO TLR3-HEK293 cells were either mock-treated or transfected with 1 μg/mL Poly(I:C) (Sigma, P1530) using Lipofectamine2000 ...
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bioRxiv - Cell Biology 2024Quote: Doxycycline-inducible stable Flp-In T-REx HEK293 cell lines expressing mitochondrial-relocated golgin-BirA* fusion proteins were induced with 1 μg/ml doxycycline (Sigma) in culture medium once they reached approximately 80% confluency ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Genomics 2020Quote: ... RRID:AB_2798177) overnight at 4°C or 1:10,000 dilution of monoclonal mouse anti-human β-actin (clone AC-74, Sigma-Aldrich, Cat. No. A2228, RRID:AB_476997) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... COCs were placed in a 100-μl droplet of human tubal fluid (HTF) medium supplemented with 4 mg/ml BSA (Sigma-Aldrich, St. Louis, MO, USA)28 ...
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bioRxiv - Cell Biology 2021Quote: ... 150 μM 4-(4-Morpholinobutylthio)phenol (MoTP) (Sigma) was added to embryos kept at 28.5°C from 24hpf onwards ...
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bioRxiv - Biochemistry 2020Quote: ... and 4×10^-4 1-Thioglycerol (Sigma Aldrich), and incubated for 72 hours ...
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bioRxiv - Neuroscience 2020Quote: ... 4-AP (4-Aminopyridine, 500 μM, Sigma-Aldrich), FK-506 (1 μM ...
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bioRxiv - Bioengineering 2019Quote: ... (ii) 4 hours of 4% sodium deoxycholate (Sigma) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Hydroxytamoxifen (4-OHT) was purchased from Sigma and dissolved in ethanol ...
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bioRxiv - Developmental Biology 2021Quote: ... 4-hydroxy tamoxifen (4-OHT, Sigma-Aldrich, H6278) treatment (1 µM in EtOH ...
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bioRxiv - Immunology 2021Quote: 4-hydroxytamoxifen (Tx) (4-OHT, #T5648, Sigma-Aldrich) was dissolved in sunflower oil to a concentration of 10 mg/mL for i.p ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μM 4-hydroxy tamoxifen (4-HT; Sigma) was added at the time of activation.
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bioRxiv - Neuroscience 2022Quote: (Z)-4-Hydroxytamoxifen (4-OHT; Sigma-Aldrich #H7904) was first prepared as 25 mg/mL stock solutions in ethanol and frozen at −20C ...