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551 - 600 of 6476 citations for B2M Cynomolgus HEK293 Flag His since 2019

• Site-specific epigenetic marks in Trypanosoma brucei transcription termination, antigenic variation, and proliferation

  Hee-Sook Kim, bioRxiv - Genetics 2021

  Quote: ... or rabbit anti-Flag (Sigma) antibodies and precipitated proteins were analyzed by western blot using mouse anti-HA (Sigma ...
• The postsynaptic MAGUK scaffold protein MPP2 organises a distinct interactome that incorporates GABA_A receptors at the periphery of excitatory synapses

  Bettina Schmerl, et al., bioRxiv - Neuroscience 2020

  Quote: ... αFlag-HRP (mouse, A8592, Sigma), αGFP (chicken ...
• A chemotactic sensor controls Salmonella-host cell interaction

  Stefanie Hoffmann, et al., bioRxiv - Microbiology 2021

  Quote: ... or Flag (M2, Sigma-Aldrich) and appropriate horseradish-coupled secondary antibodies (Dianova ...
• NSUN2-mediated m⁵C methylation of IRF3 mRNA negatively regulates type I interferon responses

  Hongyun Wang, et al., bioRxiv - Immunology 2021

  Quote: ... rabbit anti-Flag (Sigma, SAB4301135), mouse anti-m5C antibody (Abcam ...
• c-di-AMP hydrolysis by a novel type of phosphodiesterase promotes differentiation of multicellular bacteria

  Andreas Latoscha, et al., bioRxiv - Microbiology 2019

  Quote: ... Anti-FLAG primary antibody (Sigma) and the anti-mouse IgG-HRP (Thermo Fisher Scientific ...
• The E3 ligase TRIM1 ubiquitinates LRRK2 and controls its localization, degradation, and toxicity

  Adrienne E. D. Stormo, et al., bioRxiv - Molecular Biology 2021

  Quote: ... rabbit anti-FLAG (Sigma, F7425), mouse anti-myc (Sigma ...
• The Mus musculus papillomavirus type 1 E7 protein binds to the retinoblastoma tumor suppressor - implications for viral pathogenesis

  Tao Wei, et al., bioRxiv - Molecular Biology 2021

  Quote: ... or anti-Flag epitope (Sigma) antibodies coupled to agarose beads were used for immunoprecipitations followed by SDS-PAGE and western blot analysis on PVDF membranes ...
• Identification of PAX6 and NFAT4 as the transcriptional regulators of lncRNA Mrhl in neuronal progenitors

  Debosree Pal, et al., bioRxiv - Developmental Biology 2021

  Quote: p3x-FLAG-CMV-10 (Sigma) was used for the generation of PAX6 and PAX6(5A ...
• Tdrd3 regulates the progression of meiosis II through translational control of Emi2 mRNA in mouse oocytes

  Natsumi Takei, et al., bioRxiv - Developmental Biology 2021

  Quote: ... anti-FLAG (Sigma; F1804-1MG), anti-TDRD3 (Cell Signaling Technology ...
• Sequestration of TDP-43^216-414 aggregates by cytoplasmic expression of the proSAAS chaperone

  Juan R. Peinado, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-FLAG (Sigma-Aldrich, #F7425), or anti-actin (#A2228 ...
• Structural features of mTORC2 that control substrate-specific activities

  Zanlin Yu, et al., bioRxiv - Biochemistry 2022

  Quote: ... 3×FLAG peptide (F4799, Sigma), Expi29™ expression medium (A1435101 ...
• YbeY is required for ribosome small subunit assembly and tRNA processing in human mitochondria

  Aaron R. D’Souza, et al., bioRxiv - Molecular Biology 2019

  Quote: Flag 1:200 (Sigma F3165)
• Expression level of the reprogramming factor NeuroD1 is critical for neuronal conversion efficiency from different cell types

  Kanae Matsuda-Ito, et al., bioRxiv - Neuroscience 2021

  Quote: ... anti-FLAG (1:500, Sigma), anti-Tmem119 (1:500 ...
• The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin

  Dragomir B. Krastev, et al., bioRxiv - Molecular Biology 2021

  Quote: ... FLAG (M2, Sigma-Aldrich, F1804) for immunoprecipitation ...
• Target Dependent Coordinated Biogenesis Ensures Cascaded Expression of miRNAs in Activated Murine Macrophage

  Susanta Chatterjee, et al., bioRxiv - Molecular Biology 2021

  Quote: ... FLAG-M2 agarose beads (Sigma) were washed thrice with 1× IP buffer (20 mM Tris–HCl ...
• Human prefoldin modulates co-transcriptional pre-mRNA splicing

  Laura Payán-Bravo, et al., bioRxiv - Molecular Biology 2021

  Quote: ... anti-FLAG (Sigma, F3165, ChIP); anti- U2AF65 (Santa Cruz ...
• eIF4E3 Forms an Active eIF4F Complex during Stresses (eIF4F^S) Targeting mTOR and Re-Programs the Translatome

  B. Weiss, et al., bioRxiv - Molecular Biology 2021

  Quote: ... anti-FLAG (M2 antibody, Sigma), anti-4EBP1 (Cell Signalling ...
• Clr4^SUV39H1 and Bdf2^BRD4 ubiquitination mediate transcriptional silencing via heterochromatic phase transitions

  Hyun-Soo Kim, et al., bioRxiv - Molecular Biology 2021

  Quote: ... and anti-Flag (Sigma F1804) were pre-incubated with 50 μl Protein A and Protein G sepharose (GE 17513801 and 17061801 ...
• Phosphorylation by casein kinase 2 ensures ER-phagy receptor TEX264 binding to ATG8 proteins

  Haruka Chino, et al., bioRxiv - Cell Biology 2022

  Quote: ... and anti-FLAG (F4042; Sigma) antibodies ...
• A molecular brake that modulates spliceosome pausing at detained introns contributes to neurodegeneration

  Dawei Meng, et al., bioRxiv - Cell Biology 2022

  Quote: ... rabbit anti-FLAG (Sigma, F7425), mouse anti-TUBULIN (Sigma ...
• Aire-dependent genes undergo Clp1-mediated 3’UTR shortening associated with higher transcript stability in the thymus

  Clotilde Guyon, et al., bioRxiv - Genomics 2019

  Quote: ... Flag-tag M2 (F1804, Sigma), goat IgG control (sc-2028 ...
• CRISPR activation screening identifies VGLL3 and GATA2 as transcriptional regulators of PD-L1

  Ruud H. Wijdeven, et al., bioRxiv - Immunology 2021

  Quote: ... mouse anti-FLAG (Sigma, M2), rabbit anti-TEAD1 (ProSci 22-472) ...
• The GATA3 X308_Splice breast cancer mutation is a hormone context-dependent oncogenic driver

  Natascha Hruschka, et al., bioRxiv - Cancer Biology 2019

  Quote: ... anti-Flag (Sigma-Aldrich F1804), anti-HA (BioLegend HA.11) ...
• The BUBR1 pseudokinase domain promotes efficient kinetochore PP2A-B56 recruitment to regulate spindle checkpoint silencing and chromosome alignment

  Luciano Gama Braga, et al., bioRxiv - Cell Biology 2019

  Quote: ... anti-FLAG (M2, F1804, Sigma), CREST anti-centromere serum (HCT-0100 ...
• The most common RNF43 mutant G659Vfs41 is fully functional in inhibiting Wnt signaling and unlikely to play a role in tumorigenesis

  Jianghua Tu, et al., bioRxiv - Cancer Biology 2019

  Quote: ... anti-FLAG (Sigma cat # F7425), anti-β-actin (Cell Signaling cat #4970) ...
• RNF144A shapes the hierarchy of cytokine signaling to provide protective immunity against influenza

  B. Afzali, et al., bioRxiv - Immunology 2019

  Quote: ... anti-FLAG M2 monoclonal (Sigma), anti-GST (Amersham ...
• The coordinate actions of calcineurin and Hog1 mediate the response to cellular stress through multiple nodes of the cell cycle network

  Cassandra M. Leech, et al., bioRxiv - Cell Biology 2019

  Quote: ... FLAG (clone M2, F1804, Sigma), MYC (clone 9E10 ...
• Binding of guide piRNA triggers methylation of the unstructured N-terminal region of Aub leading to assembly of the piRNA amplification complex

  Xiawei Huang, et al., bioRxiv - Biochemistry 2020

  Quote: ... anti-FLAG M2 (Sigma Aldrich) (1:10K) ...
• Disease-associated scaffold protein CNK2 modulates PSD size and influences trafficking of new synaptic binding partner TNIK

  Hanna Zieger, et al., bioRxiv - Neuroscience 2019

  Quote: ... anti-FLAG-HRP (mouse, Sigma), anti-GFP (chicken ...
• EML4-ALK V3 drives cell migration through NEK9 and NEK7 kinases in non-small-cell lung cancer

  Laura O’Regan, et al., bioRxiv - Cancer Biology 2019

  Quote: ... Flag (0.5 µm/ml, Sigma), acdetylated tubulin (0.5 µg/ml ...
• Phosphorylation of Nephrin induces phase separated domains that move through actomyosin contraction

  Soyeon Kim, et al., bioRxiv - Cell Biology 2019

  Quote: ... anti-Flag (Sigma, 1:2000) to identify Nephrin) ...
• XIAP controls RIPK2 signaling by preventing its deposition in speck-like structures

  Kornelia Ellwanger, et al., bioRxiv - Immunology 2019

  Quote: ... anti-Flag M2 (F1804, Sigma). After washing in PBS ...
• ADCK4 deficiency destabilizes the coenzyme Q complex, which is rescued by 2,4-dihydroxybenzoic acid treatment

  Eugen Widmeier, et al., bioRxiv - Genetics 2019

  Quote: ... anti-FLAG M2 (Sigma-Aldrich), anti-Nidogen (Novus) ...
• Histone H2AK119 Mono-Ubiquitination is Essential for Polycomb-Mediated Transcriptional Repression

  Simone Tamburri, et al., bioRxiv - Molecular Biology 2019

  Quote: ... anti-flag (F3165; Sigma-Aldrich), anti-H3K27me1 (61015 ...
• Cyclin B1-Cdk1 binding to MAD1 links nuclear pore disassembly to chromosomal stability

  Mark Jackman, et al., bioRxiv - Cell Biology 2019

  Quote: ... FLAG (M2, Sigma; 3mg/ml) 1/1000 ...
• A tyrosine kinase protein interaction map reveals targetable EGFR network oncogenesis in lung cancer

  Swati Kaushik, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Flag (#F1804,1:1000) from Sigma and Phospho-PolI (#5095 ...
• Novel strategy for treating neurotropic viral infections using hypolipidemic drug Atorvastatin

  Suvadip Mallick, et al., bioRxiv - Microbiology 2019

  Quote: ... Flag-tag (Sigma-Aldrich, 1;1000) and β-actin (Sigma-Aldrich ...
• Global H-NS counter-silencing by LuxR activates quorum sensing gene expression

  Ryan R. Chaparian, et al., bioRxiv - Microbiology 2019

  Quote: ... and anti-FLAG-HRP (Sigma) antibodies.
• Brucella effector hijacks endoplasmic reticulum quality control machinery to prevent premature egress

  Jean-Baptiste Luizet, et al., bioRxiv - Microbiology 2019

  Quote: rabbit anti-FLAG (Sigma, #F7425) at 1/1000 ...
• Negative Regulation of Autophagy by UBA6-BIRC6–Mediated Ubiquitination of LC3

  Rui Jia, Juan S. Bonifacino, bioRxiv - Cell Biology 2019

  Quote: ... FLAG epitope (Sigma-Aldrich, F1804), SQSTM1 (for Western blotting ...
• Rrp1 translocase and ubiquitin ligase activities restrict the genome destabilising effects of Rad51 in fission yeast

  Jakub Muraszko, et al., bioRxiv - Genetics 2020

  Quote: ... anti-FLAG (1:5000, Sigma) antibodies and anti-Rad51 (1:5000 ...
• The actin nucleator Spir-1 is a virus restriction factor that promotes IRF3 activation

  Alice Abreu Torres, et al., bioRxiv - Microbiology 2021

  Quote: ... Ms anti-FLAG (Sigma F1804), Rb anti-14-3-3 (Santa Cruz ...
• Rapid characterization of spike variants via mammalian cell surface display

  Kamyab Javanmardi, et al., bioRxiv - Biochemistry 2021

  Quote: ... Anti-FLAG (Sigma-Aldrich; F3165) was also included at 1 μg/mL final concentration ...
• A combined EM and proteomic analysis places HIV-1 Vpu at the crossroads of retromer and ESCRT complexes: PTPN23 is a Vpu-cofactor

  Charlotte A. Stoneham, et al., bioRxiv - Microbiology 2021

  Quote: ... mouse anti-FLAG (Sigma-Aldrich), mouse anti-V5 (Invitrogen) ...
• The crystal structure of the varicella zoster Orf24-Orf27 nuclear egress complex spotlights multiple determinants of herpesvirus subfamily specificity

  Johannes Schweininger, et al., bioRxiv - Biochemistry 2021

  Quote: ... pAb-Flag (F7425, Sigma Aldrich), mAb-Flag-HRP (A8592 ...
• RAD51AP1 mediates RAD51 activity through nucleosome interaction

  Elena Pires, et al., bioRxiv - Biochemistry 2021

  Quote: ... α-FLAG (F3165; Sigma; 1:1,000);α-MBP (PAI-989 ...
• A Family of Glycosylated Macrolides Selectively Target Energetic Vulnerabilities in Leukemia

  Benjamin J. Reisman, et al., bioRxiv - Biochemistry 2021

  Quote: ... FLAG® (Sigma Aldrich, M2), ATP5G1 (Abcam ...
• The GARP Domain of the Rod CNG Channel’s β1-subunit Contains Distinct Sites for Outer Segment Targeting and Connecting to the Photoreceptor Disc Rim

  Jillian N. Pearring, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-FLAG (Sigma-Aldrich, F7425); pAb ...
• The GARP Domain of the Rod CNG Channel’s β1-subunit Contains Distinct Sites for Outer Segment Targeting and Connecting to the Photoreceptor Disc Rim

  Jillian N. Pearring, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-FLAG (Sigma-Aldrich, F1804); pAb ...
• Oligomerisation of THAP9 transposase: role of DNA and amino-terminal domains

  Hiral M. Sanghavi, Sharmistha Majumdar, bioRxiv - Biochemistry 2020

  Quote: ... and anti-FLAG (Sigma, F3165) primary antibodies and anti-rabbit (Thermofisher ...