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Citations for Millipore Sigma :
501 - 550 of 6476 citations for B2M Cynomolgus HEK293 Flag His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... or incubated with equilibrated FLAG beads (anti-FLAG M2 Affinity Gel, Sigma Aldrich), incubated for 2 h at 4 °C washed in a gravity column using 150 ml lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... Total Flag-sakA was detected using the Flag M2 antibody 1:2000 (Sigma) and phosphorylated SakA was detected using anti-P-p38 antibody 1:1000 (Cell Signaling) ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-FLAG M2 primary (Sigma-Aldrich, F1804) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged protein was isolated with anti-FLAG M2 magnetic beads (Sigma, M8823) for 2 hr at 4LJ°C and eluted with 100 μg/ml 3x FLAG peptide ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were purified using anti-FLAG M2 Affinity agarose gel (Sigma) for >1h at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... FLAG-tag immunoprecipitation was performed using an α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Sigma Aldrich, #F3165) and Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G ...
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bioRxiv - Microbiology 2023Quote: ... Immunoprecipitation of FLAG-tagged host factors in clarified supernatants with anti-FLAG agarose beads (EZview™ Red Anti-FLAG M2 Affinity Gel, Sigma-Aldrich) was performed at 4°C for 45 minutes ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells were cultured in DMEM-F12 (cat n. D6421, Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: HEK293 cells plated on covered with Poly-D-Lysine (P0899, Sigma-Aldrich) coverslips were co-transfected with pEZY BIFC N-Venus and C-Venus plasmids 48 hours after plating ...
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bioRxiv - Neuroscience 2022Quote: ... 0.8×105 HEK293 cells were seeded on poly-L-ornithine (Millipore Sigma) pre-coated 12 mm coverslips ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were grown in DMEM 4.5 g/L glucose (D6429, Sigma) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... while HeLa and HEK293 cancer cells were maintained in DMEM (Sigma- Aldrich). Media was supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biophysics 2023Quote: ... Stable HEK293-6E cell lines were maintained in F17 media (Sigma Aldrich) supplemented with Kolliphor p188 (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 and Caco-2 cells were grown in DMEM culturing medium (Sigma) with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... Translated FLAG-SMARCB1 was incubated with anti-FLAG M2 affinity gel (A2220, Sigma Aldrich), and then IPMK protein was added and incubated with rotation at 4°C.
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bioRxiv - Molecular Biology 2020Quote: ... Immunoblotting and immunofluorescence with a monoclonal FLAG antibody (Flag M2 Sigma, 1:100 dilution) further confirmed the endogenous knock-in of the epitope tag at the carboxy terminus of the IC3 polypeptide.
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 proteins were detected using mouse anti-FLAG M2 primary antibody (Sigma), followed by goat anti-mouse Alexa Fluor 568 (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-EGFP tagged ATP11C was eluted with 0.2 mg/ml Flag peptide (Sigma-Aldrich) in the wash buffer ...
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bioRxiv - Biochemistry 2020Quote: ... and Flag-tagged FtsX proteins were detected by using ANTI-FLAG® antibody (Sigma) as previously described by Stamsås et al ...
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bioRxiv - Microbiology 2021Quote: ... the Flag- or HA-tagged proteins were eluted with Flag peptide (Sigma, cat# F4799) or HA peptide (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... rabbit anti-flag (F7425,1:1000) and mouse anti-flag (F3165,1:1000) were from Sigma and rabbit anti-GFP (A11122,1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: FLAG Immunoprecipitation: 200 μL of 50:50 magnetic FLAG bead slurry (Millipore-Simga; M8823) were washed twice with Tris Buffer Saline (TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Rpn13(1–128)-HTP-FLAG was precipitated using M2 anti-FLAG resin (Sigma #M8823) and the precipitate was blotted for BRD2 and BRD4.
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bioRxiv - Biochemistry 2023Quote: ... Flag-tagged protein was purified using 20µl anti-Flag® M2 magnetic beads (Sigma) per plate ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse anti-FLAG (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, Cat# F3165, 1:750) was added in blocking solution for 15 minutes followed by washing with 0.05% BSA in PBS +/+ ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG and H1.0 chromatin immunoprecipitation was performed using anti-FLAG (Sigma, cat. no. F1804) and anti-H1.0 (Proteintech ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated overnight with anti-FLAG beads (anti-FLAG M2 affinity gel, Sigma Aldrich) on a rotor at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Ct226-FLAG was affinity purified using magnetic FLAG beads (Sigma-Aldrich, St. Louis, MO). Bound proteins were eluted by addition of 500ug/mL FLAG peptide (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted receptors were incubated onto a Flag affinity resin (Anti-Flag M1, Sigma) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-FLAG (Sigma, F3165) and mouse anti-αtubulin (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG (M2, F3165, Sigma-Aldrich), H2A(#07-146 ...
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bioRxiv - Cancer Biology 2021Quote: ... Flag affinity agaros (A2220, Millipore) or HA affinity agarose (A2095 ...
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bioRxiv - Cancer Biology 2021Quote: ... Flag-tag (Sigma, F3165-.2MG), Veriblot IgG (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... anti-FLAG M2 (Sigma #F1804), anti-GAPDH (Sigma #G9545) ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Flag (Sigma, Ref. F3165) incubated overnight at 1:2000 dilution followed by anti-mouse HRP (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2020Quote: ... anti-FLAG (M2 Sigma F7425), anti-lipin-1 (Cell Signaling 5195) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-FLAG (Sigma-Aldrich, F1804), anti-Nup153 (Abcam ...
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bioRxiv - Cell Biology 2020Quote: Anti-FLAG antibody (Sigma F3165) was pre-bound to protein G Dynabeads (Invitrogen 10004D ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG antibody (Sigma-Aldrich, F3165) was cross-linked to Protein G Sepherose beads ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti FLAG (F1804, Sigma), anti β actin (A-1978 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-FLAG (Sigma F1804) and mouse anti-tubulin (Sigma T5168 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse Flag (Sigma F3165). Secondary antibodies include anti-mouse HRP (Pierce) ...
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bioRxiv - Genetics 2021Quote: ... or mouse anti-Flag (Sigma), rabbit anti-H3 antibodies (Abcam).
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bioRxiv - Genetics 2021Quote: ... mouse-anti-Flag (Sigma-Aldrich), mouse anti-VSG3 (Antibody & Bioresource Core Facility ...