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Citations for Millipore Sigma :
4901 - 4950 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... and used for infection into three breast cell lines in the presence of 8 μg/ml Polybrene (Sigma-Aldrich, St. Louis, MO). Infected cells were selected with 1.5 μg/ml puromycin (Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... mixed in 1x PBS (8 ml per 10 mg) prepared from PBS tablets (Dot Scientific, Michigan) with deionized distilled water and filter sterilized (Millipore, Burlington, MA). Control animals were injected with an equivalent volume of saline made of 1x PBS ...
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bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Pathology 2022Quote: ... Then the leaves were soaked up using absorbent paper and transferred into another square Petri dish containing 8 g L-1 agar with 50 mg L-1 benzimidazole (Sigma-Aldrich, USA) for 24 hours and finally transferred to new Petri dishes and infected with Bgt #70.
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bioRxiv - Molecular Biology 2023Quote: ... 1-1.5 mL of filtered virus particles were added to the cell suspension of 50,000 cells in the appropriate media supplemented with 10% FBS and 8-10 μg/mL polybrene (Sigma # TR-1003-G) (without any antibiotics ...
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bioRxiv - Microbiology 2024Quote: ... 20-30 μg of protein for each sample was separated by 8-15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes (Millipore, cat# IPVH00010) using Trans-Blot Systems (Bio-rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... Target cells at 70-80% confluence were infected with the viral supernatant supplemented with 8 μg/ml polybrene (Sigma: TR-1003-G). Infected cells were selected using puromycin (Meilunbio ...
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bioRxiv - Neuroscience 2022Quote: ... The recombination was induced in 8-weeks old animals by intraperitoneal (i.p.) injection of 1 mg tamoxifen (100 μl; Sigma-Aldrich, Buchs, Switzerland), dissolved in sunflower seed oil/ethanol (10:1 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Determination of SL drug cytotoxicity in 2D samples was performed using the cell counting kit-8 (CCK8) viability assay (Sigma Aldrich, #96992). CCK8 uses water-soluble tetrazolium to quantify the number of live cells by producing formazan dye generated by dehydrogenases in cells ...
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bioRxiv - Neuroscience 2022Quote: ... −1.8 mm from cortical surface) using glass microcapillary (tip diameter ≃35 μm; 1–5 μL Hirschmann® microcapillary pipette, Z611239, Sigma-Aldrich) connected to a custom Openspritzer pressure system (Forman et al. ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing lentiviral particles was filtered through a 0.45 µM membrane (Fisher, 9720514) and was supplemented with 8 µg/µL polybrene (Sigma, TR-1003-G) prior to infection ...
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bioRxiv - Molecular Biology 2023Quote: ... and chromatin was either digested for 4 min for nChIP or 8 min for ChIP-seq with 1.0 U MNase (Sigma Cat #N3755-500UN) in nuclei solubilized with 1 mL of 0.1 M TE (10 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... the dried protein pellet was dissolved in 50 µL of 8 M urea (Cytiva, 17131901) in 100 mM NH4HCO3 (ABC; Sigma-Aldrich, 11213) and 5 mM dithiothreitol (DTT ...
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bioRxiv - Genetics 2024Quote: ... The recipient cells were infected 48 hours post-transfection of HEK 293 cells with the virus supernatant in the presence of 8 μg/ml polybrene according to the manufacturer’s guidelines (Sigma-Aldrich, MO, USA). The cells transduced with the pLVX-Tight-Puro-FoxM1-dNdK were selected in 2 μg/ml puromycin (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Vessel patency and intravascular flow was confirmed between days 6-8 by addition of sterile 50µg/mL 70kDa FITC- or rhodamine-conjugated dextran (Sigma #FD70S or #R9379) to the media reservoirs ...
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bioRxiv - Immunology 2023Quote: ... viral transduction was performed by spin-infection with 2,000 g at 33 °C for 2 hours in the presence of 8 μg/ml polybrene (Sigma-Aldrich, cat# H9268), followed by incubation for another 4 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... 210,000 cells each of asynchronous and mitotic cells were seeded (same density as 60,000 cells per well of the 8 chambered cover glass) onto a 5 μg/mL Humanplasma Fibronectin (Sigma Aldrich,1mg/mL) coated in PBS (1 hour at 37℃ ...
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bioRxiv - Developmental Biology 2022Quote: ... WT/IWR consists of 9 wild type embryos and 8 embryos treated with the small molecule inhibitor 10µM IWR1 (Sigma Aldrich, Cat I0161). DMSO/DMH4 includes 12 embryos at 3 dpf ...
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bioRxiv - Neuroscience 2023Quote: ... Whole purified His-MBP-ApoE4M was concentrated to a final concentration of ∼ 8 mg/ml using Centrifugal Filter Units AmiconR Ultra-15 UltracelR-3K (Merck Millipore Ltd., Ireland). The crystallization was performed in Easy-Xtal 15-well crystallization plates by a hanging drop vapour diffusion ...
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bioRxiv - Pathology 2023Quote: ... Cells were infected with lentivirus carrying shHDAC6/shATG5 or shControl at MOI 5 for 24 hours in the presence of Hexamethidine Bromide (8 µg/mL; Sigma-Aldrich - H9268). Transduced cells were selected using puromycin (Sigma Aldrich #P9620 ...
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bioRxiv - Microbiology 2023Quote: ... Transductions were performed via spinoculation at 1100xg for 90 min at 30C in the presence of 8 µg/mL protamine sulfate (Millipore Sigma #P3369). Cells were nucleofected with SLC35A2 RNPs two days later ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated for two weeks (treatments replenished at days 4, 8 and 11) prior to addition of 0.5% crystal violet (Sigma-Aldrich, Cat#C0775) in 50% methanol for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... sonicated for 2 minutes of 2 sec on/8 seconds off cycles on ice using probe sonicator (Ultrasonic processor 500W, Sigma Aldrich, USA). 3-5 mL of 5 % NaCl was added to the samples and after thorough vortexing the phases were separated by centrifugation at 3,000 × g for 5 min ...
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bioRxiv - Microbiology 2023Quote: Cellvis 8-well (Mountain View, CA, USA) coverslips were pre-coated with laminin (20 µg/mL, Sigma Aldrich, St. Louis, MO, USA) for 1 h at 33 °C and washed twice with ddH2O ...
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bioRxiv - Cancer Biology 2023Quote: ... Addgene_17446)] at a multiplicity of infection of 10 with 8 μg/mL polybrene (hexadimethrine bromide [Cat # 107689, Sigma Aldrich, St. Louis, MO, USA) for 24 hours before washing with PBS and replacing with fresh medium (49) ...
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bioRxiv - Cancer Biology 2023Quote: ... were spin transduced with 10-20μl of the concentrated virus at 37°C at 2200g for 90 minutes with polybrene reagent (8-10μg/ml, Millipore Sigma, Billerica, MA). The cells were washed with PBS and resuspended in fresh RPMI media supplemented with 10% FBS with 1% penicillin and streptomycin 24 hours post-transduction ...
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bioRxiv - Genomics 2023Quote: ... large tissue pieces were spun down in a microcentrifuge and the supernatant containing single cells was passed through a 70-um cell strainer fitted onto a 50-mL conical tube containing 8 mL DPBS + 1% bovine serum albumin (BSA; Sigma-Aldrich, A7906) in DPBS on ice ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... 1.8 µg/cm2) coated hanging culture inserts (Millicell Hanging Cell Culture Insert, polyethylene terephthalate, 1.0 µm pore size, EMD Millipore, MA, USA) and maintained at 37°C in 5 % CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... C2C12 cells were seeded in a 6 cm dish and 105-106 virus particles were added dropwise to media containing 8 ug/mL Sequabrene (Sigma S2667-1VL) and 25 mM Hepes buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... were dissected from distal regions of the lung and placed on Nucleopore Track-Etched Membrane disks (13 mm, 8 µm pore, poly- carbonate) (Sigma, Cat#WHA110414) floating on top of 500 µl of human lung ALI explant media (Advanced DMEM/F-12 (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: ... then transferred to a microcentrifuge tube on ice which had previously been coated with bovine serum albumin (BSA) by washing with 0.5 mL of 10% (v./v.) BSA (Sigma 9048-46-8) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Developmental Biology 2023Quote: ... the desired number of embryos were aggregated at the uncompacted 8-cell stage and cultured together in a single microdroplet of global® medium under mineral oil (Sigma, M8410). Embryo aggregation was encouraged by placing them in contact with each other in micro-indented wells in 3.5mm petri dishes (Falcon ...
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bioRxiv - Biophysics 2023Quote: ... the master SU-8 2050 photoresist was treated with trichloro(1H,1H,2H,2H-perfluorooctyl)silane (448931, Sigma-Aldrich, Saint Louis, MO) in a desiccator for overnight ...
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bioRxiv - Plant Biology 2023Quote: ... cerevisiae was isolated from liquid YPD cultures 8 hours after inoculation from single colonies using the formamide-EDTA method (59) followed by RNAzol® RT (Sigma-Aldrich) extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... were resuspended 2 mL of viral particle supernatant for E:T of 2:1 with 8 μg/mL of Polybrene (Millipore Sigma, Burlington, MA), maintained in at 37°C for 4 hours ...
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bioRxiv - Biochemistry 2023Quote: ... the glycopeptides were heat treated to inactivate the trypsin and the sample was subject to treatment with PNGase F (250U, Sigma; pH 8) followed by PNGase A (NEB ...
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bioRxiv - Cancer Biology 2023Quote: Whole cell protein lysates were obtained by lysing cell pellets on ice in NP-40 buffer (150mM NaCl, 1% NP-40, 50mM tris pH 8) with 10x Protease Inhibitor Cocktail (Sigma-Aldrich, P8340). Protein quantification was done using the Pierce™ BCA Protein Assay Kit (Thermo Scientific ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... viability measurements were performed for the 1 vpc and 3 vpc conditions of all vectors and two wells of uninfected cells with the Cell Counting Kit 8 (CCK8, Sigma-Aldrich #96992). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... Sub-confluent A549 cells were subsequently transduced with 2 mL of the filtered lentivirus-containing supernatant supplemented with 8 µg/mL polybrene (Sigma-Aldrich; 107689) for 24h ...
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bioRxiv - Cell Biology 2024Quote: 3T3-L1 cells were grown in 6-well plates until 50-60% confluency before the medium was replaced by the concentrated virus mixture supplemented with 8 µg/mL polybrene (Sigma-Aldrich, #107689). After overnight incubation the medium was replaced by DMEM supplemented with 10% CS ...
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bioRxiv - Cell Biology 2024Quote: ... E25PV4550S) and added to HeLa-TetON-WTOTC cells (65) cultured in a media supplemented with 8 μg/mL polybrene (Sigma Aldrich, H9268). 48 hours later ...
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bioRxiv - Molecular Biology 2024Quote: ... passed through a 0.45 µm filter and used to infect either HEK293 cells in a dilution of 1:5 or RAW macrophages in a dilution of 1:2 with 8 µg/ml polybrene (Sigma-Aldrich, TR1003G). Transduced cells were selected by either puromycin (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2024Quote: ... transferred onto nitrocellulose membrane (Amersham #10600041) in transfer buffer (8 mM Tris, 30 mM Glycine, 20 % Methanol) and stained with Ponceau S (Sigma-Aldrich, #P7170). Membranes were blocked in TBS-T + 5% BSA for 1 hr at RT and incubated overnight at 4°C in primary antibodies prepared in TBS-T + 5% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell infection was performed by adding lentiviral particles to cell lines in the presence of 8 μg/ml polybrene (Sigma, TR-1003) for 12 h followed by fresh media addition ...
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bioRxiv - Neuroscience 2024Quote: ... drugs were diluted in AHL saline to the following final concentrations: 100 µM Picrotoxin (P1675-1G, Sigma-Aldrich, CAS 124-87-8), 50 µM CGP54626 hydrochloride (1088/10 ...
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bioRxiv - Immunology 2024Quote: ... Concentrated virus particles solubilized in IMDM were applied to HL60 cells in the presence of 8 μg/ml polybrene (Sigma-Aldrich #H9268). Clones expressing sgRNAs were selected with 2 μg/ml puromycin ...
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bioRxiv - Microbiology 2024Quote: ... lungs were carefully removed from the hydrogel and rinsed in 0.1M PBS and transferred to clearing solution (8% sodium dodecyl sulfate, SDS, Sigma-Aldrich Inc, USA) in 0.1M PBS containing 50mM sodium sulfite (Sigma-Aldrich Inc ...
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bioRxiv - Molecular Biology 2024Quote: ... then stored in sterilized polypropylene bottles (Nalgon, Cat. No. 2330) at 8° C until filtered using 0.22 µm Sterivex filters (Merck-Millipore, Cat. no. SVGP01050) in 60 mL increments using disposable syringes ...
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bioRxiv - Microbiology 2024Quote: ... Then 8 µL samples were spotted on a silicon disk coated with 0.01% (w/v) poly-L-lysine (Merck Millipore, Billerica, MA, USA). Cells were fixed with 2.5% (v/v ...