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Citations for Millipore Sigma :
4851 - 4900 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were grown on vitronectin coated plates containing PluriSTEM® Human ES/iPS Cell Medium (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... human Spindly siRNA (Sequence antisense: 5’-GAAAGGGUCUCAAACUGAA-3’, dTdT overhangs, Sigma-Aldrich, St. Louis, MO, USA), and control siRNA (D-001810-10-05 ...
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bioRxiv - Pathology 2024Quote: ... the cells were cultured on culture dishes coated with 0.05mg/ml human placenta derived COLIV (Sigma) in Bronchial epithelial cell growth medium with added supplements according to the manufacturer’s recommendation (BEBM-Lonza ...
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bioRxiv - Cancer Biology 2024Quote: Tumors were harvested as mice reached human endpoint and fixed in neutral buffered formalin (Sigma-Aldrich) for 24-48h ...
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bioRxiv - Cell Biology 2024Quote: MISSION shRNAs against human KIF1A (SHCLNG-NM_004321) and KIF5A (SHCLNG-NM_004984) were obtained from Sigma-Aldrich. Scramble vector was the MISSION pLKO.1-puro non-Mammalian shRNA Control vector ...
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bioRxiv - Immunology 2024Quote: ... 10 μg/mL dsDNA or 10 μg/mL recombinant human insulin (Sigma Aldrich, St. Louis, MO). After five washes in PBS with 0.05% Tween 20 ...
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bioRxiv - Neuroscience 2024Quote: Glycated Aβ (gAβ) was prepared by incubating 10 mg/ml of human Aβ1-42 (Sigma-Aldrich) with or without 0.5 mM methylglyoxal (MGO ...
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bioRxiv - Microbiology 2024Quote: Primary human foreskin fibroblasts (HFFs) (ATCC) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) with 4.5 g/L glucose ...
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bioRxiv - Immunology 2024Quote: ... Plates were then washed and incubated with HRP-conjugated goat anti-human IgG antibody (Sigma-Aldrich) (diluted 1:2500 in PBS-T ...
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bioRxiv - Molecular Biology 2024Quote: 50,000 human iPS-CM were seeded/well in Lab-Tek 4-well chamber slides (Sigma-Aldrich) and cultured for five days according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... HGF concentration in the CM was determined using the human HGF ELISA Kit (Sigma-Aldrich #RAB0212) following manufacturer protocol.
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bioRxiv - Cell Biology 2020Quote: ... At the end of the 3-hour incubation period cells were washed 3x PBS and lysed for western blot in lysis buffer (20 mM Tris pH 8, 10% glycerol, 135 mM NaCl, 0.5% NP40, 2.5 mM MgCl2, DNAse (Sigma-Aldrich, cat# DN25-1G), 1x protease inhibitors (cOmplete Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2019Quote: ... and glucose uptake was initiated by adding 2 ml serum-free and glucose-free DMEM containing 2-[1,2-3H(N)]-2-deoxy-D-glucose (DG) (NET328, specificity 8 Ci/mmol) at 1µCi/ml and 10 mM unlabeled-2-DG (Sigma, Catalog #: D-3179) to each sample in the absence and presence of 20 µM cytochalasin B (Sigma Catalog # ...
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bioRxiv - Developmental Biology 2021Quote: Recombination of the conditional allele was induced in Atxn10flox/flox;CAGG-creERT2 mice at 6 and 8 weeks old by a single intraperitoneal (IP) injection of 9mg tamoxifen (Millipore Sigma, T5648) per 40g (body weight ...
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bioRxiv - Neuroscience 2020Quote: R6/1 mice were treated (from 8 to 20 weeks of age) with vehicle (90% water, 10% Polysorbate 80) or betulinic acid (50 mg/kg; Sigma-Aldrich #855057) administered by oral gavage ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... plants grown under a long day photoperiod (16 h light/8 h dark, 60% humidity) using the SpectrumTM Total RNA extraction kit (Sigma-Aldrich, USA). The sample was enriched for mRNA using the Poly(A ...
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bioRxiv - Genetics 2019Quote: ... Western blots were probed using anti-GFP (Ozyme; #JL-8; 1/1,000) and anti-tubulin (to confirm equal loading) (Sigma, #DM1A, 1/5,000) antibodies ...
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bioRxiv - Biochemistry 2019Quote: apoTaLPMO9A was prepared by dialyzing the purified TaLPMO9A extensively using D-tube Dialyzer Maxi (6-8 kDa) from Millipore (Billerica, MA, USA). Dialysis was performed in 20 mM phosphate buffer (pH 7 ...
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bioRxiv - Neuroscience 2019Quote: ... treatment consisted of an intraperitoneal injection of either the TrkB agonist 7,8-Dihydroxyflavone (7,8-DHF) (98% Sigma Aldrich CAS#: 38183-03-8) (10mg/kg ...
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bioRxiv - Neuroscience 2019Quote: ... well-fed L3 worms were mounted on 1% agarose pads with 5 mM sodium azide (Sigma-Aldrich, CAS No. 26628-22-8), whereas dauers were mounted on pads with 9 mM sodium azide (Simpkin and Coles ...
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bioRxiv - Bioengineering 2019Quote: ... Hela cells from the same batch of 2D culture were seeded into the peptide hydrogel with density ~ 8 × 104 which was measured by an automated cell counter (Millipore Corporation, PHCC3000). The 3D culture was maintained using a 24-well cell culture plate with ~ 390 μL cell suspension ...
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bioRxiv - Immunology 2019Quote: ... The inhibition of ADAM-17 (a disintegrin and metalloprotease-17) was performed by adding 8 μM TAPI-1 (Merck Millipore, Burlington, MA). TAPI-1 and MK2 IV were added 60 minutes before cytokine stimulation ...
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bioRxiv - Cell Biology 2020Quote: ... The following day cells were treated for 8 h with either ES DMEM-2i + DMSO or ES DMEM-2i + CHX (100 ug/ml (Sigma-Aldrich, 01810)) ...
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bioRxiv - Cell Biology 2021Quote: ... RPE1-hTERT cells were transduced with the viral supernatant from the transfections in the presence of 8 ug/mL polybrene (Millipore, Burlington, MA) for 24 hr ...
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bioRxiv - Cell Biology 2021Quote: ... the same amount of suspended cells were added into upper chambers of Corning Costar Transwell cell culture inserts (pore size 8 µm; Sigma-Aldrich, #CLS3464) with the coating of 30 μg MatrigelTM Basement Membrane Matrix (BD Biosciences) ...
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bioRxiv - Plant Biology 2021Quote: ... mixtures consisted in 2 μl of diluted template and 8 μl of FastStart Universal SYBR Green Master (Rox) (Cat. # 04913914001, Sigma-Aldrich Argentina). Reactions were set in technical duplicates or triplicates in MicroAmp® Fast Optical 48-well 0.1 mL reaction plates (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... animals at 8 weeks of age were gavaged with 100 µL of a 30 mg/mL solution of tamoxifen/corn oil (Sigma Aldrich T5648). This was done 3 consecutive days then animals were allowed to rest for 4 days before another three consecutive daily doses (6 doses over 10 days) ...
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bioRxiv - Immunology 2021Quote: ... Fab samples were concentrated to ~6-8 mg/ml and buffer exchanged to TBS using Amicon ultrafiltration units with a 10 kDa cutoff (EMD Millipore Sigma). Final Fab yields were ~1 mg ...
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bioRxiv - Biophysics 2020Quote: ... 304 μL BPTI (4 different concentrations ranging from 5.2 to 52.6 μM) was mixed with 8 μL of the substrate Z-GPR-pNA (Sigma-Aldrich, St. Louis, MO) (5 different concentrations ranging from 0.4 to 10 mM) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant containing lentiviral particles was filtered through 0.45 µM membrane (Fisher, 9720514) and was supplemented with 8 µg/µL polybrene (Sigma, TR-1003-G) prior to infection ...
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bioRxiv - Pathology 2022Quote: ... Twenty micrograms of protein per lane were loaded onto an 8-12% polyacrylamide Gel and then transferred to PVDF membranes using an electrophoresis chamber (Millipore, Shanghai, China) for 1.5 h ...
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bioRxiv - Biochemistry 2020Quote: ... The Legionella pneumophila DrrA 8-533aa (referred to as DrrA)-encoding DNA was cloned into a modified pET19 vector (Merck Millipore, Burlington, Massachusetts) as previously described (Müller et al. ...
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bioRxiv - Neuroscience 2021Quote: Microtissues were fixed within the agarose-molded wells of the tissue culture plate with cold PFA solution (4%(w/v) paraformaldehyde, 8% (w/v) sucrose) (F79-500, Sigma-Aldrich) (S7903, Sigma-Aldrich). Microtissues were fixed for 2 hours followed by 3 PBS washes ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was homogenized in a lysis buffer (20 mMTris-HCl pH=8; 137 mMNaCl; glicerol 10%) supplemented with protease inhibitor (Sigma-Aldrich, USA), and then centrifuged at 10.000g for 15min to the subsequent supernatant storage in −80°C ...
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bioRxiv - Neuroscience 2020Quote: ... Hair cells were inactivated by the flushing of 8-10 drops of 100 mg/mL neomycin sulfate (Sigma-Aldrich, St Louis, MO) through the punctured round window membrane whilst aspirating from the apex via a small hand-drilled hole ...
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bioRxiv - Biochemistry 2021Quote: ... 12 μg peptides were adjusted to pH 7-8 by adding one volume of 50 mM ammonium bicarbonate (ABC, Sigma-Aldrich, A6141). Offline fractionation by high-pH reversed-phase chromatography was carried out on an Ultimate 3000 HPLC (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Table 2) and were introduced by 4 lentiviral infections at 12hr intervals in presence of 8 μg/ml polybrene (Sigma-Aldrich®) using supernatant from transfected 293T cells ...
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bioRxiv - Developmental Biology 2022Quote: ... Ovaries were obtained from B6D2F1 female mice (8–10 weeks old) 48 hours after PMSG injection and were then transferred to M2 medium (Sigma-Aldrich, M7167) supplemented with 0.2 mM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Immunology 2019Quote: ... Cultured cell lysates (30 μg) were electrophoresed in an 8-12% SDS-PAGE gel and transferred to a PVDF membrane (Millipore, MA, USA). PVDF membranes were blocked with 5% skim milk in phosphate buffered saline with 0.1% Tween 20 (PBST ...
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bioRxiv - Neuroscience 2020Quote: A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
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bioRxiv - Microbiology 2021Quote: Oral microgavage of 6 dpf larval zebrafish was performed using pH indicators m-cresol purple (0.2%; Thermo-Fisher, 2303-01-7) or phenol red (2%; Sigma, 143-74-8). The fish were then imaged on a Leica MZ10F stereoscope using a Leica MC190 HD color camera ...
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bioRxiv - Immunology 2020Quote: ... Cultured cell lysates (30 μg) were electrophoresed in an 8–12% SDS-PAGE gel and transferred to a PVDF membrane (Millipore, MA, USA). PVDF membranes were blocked with 5% skim milk in phosphate buffered saline with 0.1% Tween 20 (PBST ...
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bioRxiv - Synthetic Biology 2020Quote: ... and blocked for 1h in 3% BSA (Fisher bioreagents CAS 9048-46-8) and 0.1% Triton X-100 (Sigma 9002-93-1). The cells were then incubated in primary antibody overnight ...
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bioRxiv - Pathology 2020Quote: ... deletion of CCN2 or STOP in reporter td Tomato using Acan CreERT2 was induced at 8 weeks of age using tamoxifen (Sigma Aldrich, UK). All mice ...
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bioRxiv - Biophysics 2020Quote: ... The otolithic membrane was gently removed after 8-min enzymatic dissociation with 50 μg/mL Collagenase IV (Sigma Aldrich, St. Louis, MO). For experiments in which calcium concentration of endolymph was varied ...
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bioRxiv - Cancer Biology 2021Quote: ... we used a Boyden chamber which consists of cell culture insert containing 8-μm pore polyethylene terephthalate membrane (EMD Millipore, Burlington, MA). The inserts were seated in 24-well companion plates ...
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bioRxiv - Cell Biology 2021Quote: ... 8% SDS-PAGE was used for protein separation by electrophoresis and transferred to a PVDF membrane (Immobilon®-P, Millipore Corporation, IPVH00010). 10% BSA was used to block the membrane followed by protein detection using primary antibody of 1:1000 dilution such as anti-β actin (C4 ...
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bioRxiv - Developmental Biology 2020Quote: Fetal hearts were collected at E11 from uninfected and CVB3 infected (E9) dams and cultured on Millicell EZ 8-well slides (Millipore Sigma PEZGS0816) in high-glucose DMEM (ATCC 30- 2002 ...
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bioRxiv - Neuroscience 2021Quote: ... Y1eGFP mice at 6-8 weeks of age were transcardially perfused with chilled phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (Sigma-Aldrich, USA) in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The column was washed with 10 column volumes of wash buffer and proteins were eluted with 8 ml of the same buffer containing 10 mM maltose (Sigma-Aldrich M5895). The eluate was applied to a HiLoad 16/600 Superdex200 pg column (GE Healthcare 28-9893-35 ...