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Citations for Millipore Sigma :
4901 - 4950 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... proteins were reduced with 10 mM dithiothreitol (Sigma Aldrich, UK) in ammonium bicarbonate for 30 min at 37°C and alkylated with 55 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were precleared with protein A-Sepharose beads (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... carrier protein and 0.2 mL of 100% Trichloroacetic acid (Sigma) was added to the extracted protein fraction ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were then transferred to polyvinylidene difluoride (PVDF) membranes (Millipore) using a semi-dry transfer apparatus at 15 V for 30 minutes (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... protein aliquots were incubated with 10μg of free avidin (Sigma) per 1mg of input protein for 1h at 4°C with constant rotation ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were reduced in 10 mM dithiothreitol (Sigma Aldrich, UK) for 30 min at 37°C and alkylated in 55mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Separated proteins were transferred to PVDF membranes (Millipore, Billerica, MA) and probed with appropriate dilutions of primary antibody followed by appropriate dilutions of horseradish peroxidase-conjugated secondary antibody ...
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bioRxiv - Biophysics 2020Quote: ... Buffer proteins were purchased from Sigma-Aldrich (St. Louis, MO), and salts were purchased from Thermo Fisher Scientific.
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bioRxiv - Biophysics 2021Quote: ... Protein bands were detected with either anti-Flag (Sigma-Aldrich) or anti-His antibodies (Sigma- Aldrich).
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bioRxiv - Molecular Biology 2021Quote: ... Mpro proteins were concentrated to 25 mg/ml by Millipore’s ultrafiltration columns and a final concentration of 1 mM DTT was added and stored at −80°C.
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bioRxiv - Developmental Biology 2021Quote: For Hyaluronic Acid-Binding Protein (HA-BP, Sigma-Aldrich, 385911) immunofluorescence ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Protein expression was induced with 0.1% L-arabinose (Sigma-Aldrich). Expression took place for 16 h at 15°C and 220 rpm ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.25x Protease Inhibitor Cocktail for Histidine-tagged proteins (Sigma-Aldrich), and 0.3 mg/mL lysozyme ...
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bioRxiv - Systems Biology 2020Quote: ... blots were stained with Ponseau S total protein stain (Sigma) prior to blocking at room temperature (5% skim milk ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were blotted on PVDF membrane (Millipore Immobilon-P, #IPVH00010) at 100 V for 1 h at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... The filtrates were then concentrated in protein concentration columns (Millipore CENTRICON YM-10 Centrifugal Filter Unit 2 mL 10 kDa ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were wet-transferred to PVDF membranes (Immobilon-P, Millipore), blocked in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto Immobilon-FL PVDF membranes (Millipore, #IPF00010) for 2 h at 30 V on ice ...
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bioRxiv - Biochemistry 2021Quote: Protein was labeled with Fluorescein Isothiocyanate (FITC, Merk, Sigma-Aldrich) according to the supplier’s instructions with slight modifications ...
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bioRxiv - Molecular Biology 2021Quote: Protein mixtures were precipitated with TCA (Sigma Aldrich, Cat# T0699) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fusion proteins were captured using V5 agarose beads (Sigma # A7345) that were pre-cleared overnight with PBS + 0.1% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... pre-cleared with 40 μl Protein A agarose beads (Millipore), and then immunoprecipitated with specific antibodies against REST/NRSF (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... the proteins were concentrated with Amicon Ultra 4 MWCO10kDa (Millipore) to approximately 3 – 4 mL ...
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bioRxiv - Molecular Biology 2019Quote: The protein was concentrated with Amicon Ultra 4 MWCO30kDa (Millipore) at 20 °C at 1650 × g ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were reduced with 2μl of 0.2M dithiothreitol (Sigma) for one hour at 57 °C at pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were transferred to a nitrocellulose membrane (Merck Millipore). The membrane was then incubated with 5% BSA in Tris-buffered saline with tween 20 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then transferred onto a nitrocellulose membrane (Millipore). The membranes were blocked using Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Microbiology 2020Quote: ... lysed in BugBuster Primary Amine Free Protein Extraction Reagent (Novagen) supplemented with recombinant lysozyme ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein complexes were eluted with 3xFlag peptide (200ug/mL, Sigma) in 5 column volumes Buffer 2 for 1 hour at 4C ...
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bioRxiv - Systems Biology 2020Quote: ... proteins were transferred to Immobilon FL PVDF membrane (Merck-Millipore) using semi-dry transfer (25 V for 30 min.) ...
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bioRxiv - Biophysics 2019Quote: ... then bound proteins were eluted with 3 mM desthiobiotin (Sigma) or 50 mM D-biotin (CHEM-IMPEX ...
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bioRxiv - Cell Biology 2019Quote: ... protein inhibitor and phosphatase inhibitor (Complete-EDTA free, Roche/Sigma prod ...
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bioRxiv - Microbiology 2019Quote: ... 20 µL protein A agarose suspension (Calbiochem/Millipore #IP02-1.5ML) was added to each and samples were rocked at 4°C for 2 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... The proteins were then transferred on PVDF membrane (Millipore, USA) by overnight wet transfer at 30 volts ...
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bioRxiv - Microbiology 2020Quote: ... Protein quantification was performed using the Bradford Reagent (Sigma-Aldrich). Equal amounts of protein lysates were separated by SDS-PAGE and proteins were transferred to nitrocellulose filters ...
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bioRxiv - Cell Biology 2019Quote: ... Relative protein loading was visualized using Ponceau S Solution (Sigma). Membranes were subsequently washed in TBST and blocked for 1 hour in 5% skim milk in TBST at RT ...
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bioRxiv - Systems Biology 2019Quote: ... proteins were reduced by adding 10 mM DTT (Sigma Aldrich) prior to alkylation with 55 mM iodoacetamide (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins were transferred onto a polyvinylidene fluoride membrane (Millipore). The membrane was blocked with 3% BSA/Tris-buffered saline that contained 0.1% Triton X-100 (TBST ...
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bioRxiv - Genetics 2019Quote: ... conjugated on Protein A+G Magnetic beads (Millipore, 16-663). Immunoprecipitated DNA was amplified by PCR and subjected to Illumina sequencing.
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bioRxiv - Zoology 2019Quote: ... Hsp70 protein was labelled with anti-HSP70 (Sigma-Aldrich, # H9776) at 1:3000 ...
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bioRxiv - Immunology 2019Quote: ... and the antibody was purified with Protein G (Sigma-Aldrich). Mice were injected every 2 days for a total of 9 injections with 1.5 μg of the anti-c-Fms antibody in 3 μL of phosphate-buffered saline (PBS) ...
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bioRxiv - Physiology 2019Quote: ... followed by determining protein concentration using BCA assay kit (SIGMA). Samples were loaded onto SDS-PAGE for protein expression analysis.
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bioRxiv - Molecular Biology 2020Quote: 20 μg protein was incubated in 5x Laemmli buffer (Sigma) at 95°C for 10 min and separated by a Mini-PROTEAN® TGX (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were transferred to PVDF membranes (Immobilon-P, Millipore) and probed with antibodies to detect the FLAG fusions and endogenous proteins ...
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bioRxiv - Biochemistry 2019Quote: ... Protein concentrations were determined using the Bradford assay (Sigma-Aldrich) with bovine serum albumin as standard.
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bioRxiv - Systems Biology 2019Quote: ... Separated protein lysates were transferred to PVDF membranes (Millipore #IPVH00010), which were subsequently rinsed in TBST (Tris-buffered saline ...
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bioRxiv - Genetics 2019Quote: ... Proteins were transferred from gels to Immobilon-P membrane (Millipore) and probed with antibodies as described 20 ...
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... and protein was quantified using a Bradford assay kit (Sigma) following manufacturers’ instructions.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore), and blocked with 5% skim milk in TBS-0.1% Tween20 (TBST ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using the Direct Detect assay (Millipore). Protein samples were run on 4–15% Mini-PROTEAN TGX gels (Bio-Rad) ...