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Citations for Millipore Sigma :
4851 - 4900 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Parasites were maintained in fresh human erythrocytes at 2% hematocrit in complete RPMI-1640 (Sigma) medium in addition to 0.5% AlbuMaxII (Gibco by Life Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... was from Thermo Fisher Scientific (Waltham, MA) and human tissue plasminogen activator (tPA) from Sigma Aldrich (St. Louis, MO). Rabbit monoclonal anti-PAI-1 [EPR17796] (ab187263 ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence or absence of 100 pg/ml recombinant human (rh)-IL21 (Sigma-Aldrich). The next day ...
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bioRxiv - Biophysics 2023Quote: ... The anti-human Fc capturing mAb (GG-7) was from Sigma-Aldrich (St. Louis, MO). LIBS-2 was purchased from EMD Millipore (Billerica ...
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bioRxiv - Cancer Biology 2023Quote: ... DMEM medium supplemented with 10% human serum (male AB, H6914-100ml Batch SLBT2873, Sigma-Aldrich), 1 % penicillin/streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: Lentiviral particles encoding Flag-tagged human PDHA1 or shRNAs targeting mouse Pdha1 (TRCN0000041914/TRCN0000041914 (Sigma)) were produced in 293T packaging cells by transient transfection using Jet-PEI reagent (Ozyme) ...
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bioRxiv - Immunology 2022Quote: ... human CD3+ T cells were stimulated with 10ng/ml PMA and 1μM Ionomycin (Sigma-Aldrich) or with 1 μg/mL pre-coated α-CD3 and 1 μg/mL soluble α-CD28 ...
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bioRxiv - Cell Biology 2023Quote: ... Human colorectal adenocarcinoma cells (Caco-2, ATCC) were maintained in medium consisting of DMEM (Sigma), 100 U/ml penicillin ...
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bioRxiv - Developmental Biology 2023Quote: ... treated twice every 12 h with human recombinant sDLL4 (1µg/ml, Cat#: SRP3026, Sigma Aldrich) for a total of 24h ...
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bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was incubated in Sudan Black (0.3% Sudan Black B (Sigma, Cat# 199664) in 70% ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µL of anti-human IgG (Fc specific)-peroxidase antibody produced in goat (Sigma, A0170) was added to each sample in a 1:40,000 dilution of PBS and incubated for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA encoding human PF4 ORF was cloned into the pET-15b vector (Novagen, Madison, WI) and transformed into Origami B (DE3 ...
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bioRxiv - Immunology 2023Quote: ... diluted with a 10x volume of RPMI with 10% heat-inactivated human AB serum (Sigma), and centrifuged at 300 x g for 7 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Primers for human H19 were used as a positive control for CTCF binding (Merck Millipore).
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bioRxiv - Cell Biology 2023Quote: Human αSN cDNA was transformed into Escherichia coli Strain BL21 (DE3) (Novagen, Madison, Wis., USA) using the pNIC28-Bsa4 plasmid ...
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bioRxiv - Genomics 2023Quote: ... 3.33 ml 75 mg/ ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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bioRxiv - Biochemistry 2023Quote: ... sub-cultured until mid-exponential phase then opsonized in human serum (Sigma-Aldrich, no. H4522) for 30 min ...
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bioRxiv - Immunology 2023Quote: Human macrophages were harvested from culture using PBS+5mM Ethylenediaminetetraacetic acid (EDTA; Sigma Aldrich #E7889) and cell lifters (Fisher Scientific #08-100-240) ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blotted with the following antibodies: anti-human PSEN1-CTF (MAB5643; Merck Millipore); anti-PEN2 (D6G8 ...
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bioRxiv - Immunology 2023Quote: ... 20 mg of purified human IgG in PBS was immobilised to CNBr-activated Sepharose (Sigma) (10 mg IgG/ml packed Sepharose) ...
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bioRxiv - Microbiology 2023Quote: ... Monocytes were selected by adhering PBMCs in RPMI with 5% pooled AB human serum (Sigma), 1 mM HEPES (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... AS Diagnostik) and polyclonal rabbit anti-human FHL2 (2 µg/mL, HPA006028, Sigma/Prestige Antibodies) was performed at 4 °C overnight in PBS/10% AB serum ...
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bioRxiv - Biochemistry 2024Quote: ... Human CXCL12 [50 ng/mL] and EGF [50 ng/mL] (Sigma Aldrich SRP3276 and SRP6253) were prepared in serum-free DMEM and used as chemoattractant in the lower chamber ...
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bioRxiv - Bioengineering 2024Quote: ... 10 µg/L human epidermal growth factor (hEGF; E9644, Sigma-Aldrich, St. Louis, MO, USA), 1% ITS-X supplement (094-06761 ...
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bioRxiv - Microbiology 2024Quote: ... TSB-grown bacteria were centrifuged and resuspended in human serum from male AB plasma (Sigma) and incubated for 16 h at 37 °C with shaking (180 rpm) ...
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bioRxiv - Immunology 2024Quote: ... which were coated with human placental type IV collagen (Sigma-Aldrich, St. Louis, MO; C7521). Ex Plus medium was added to both the apical and basolateral compartments and changed daily until the cells reached confluency on the inserts ...
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bioRxiv - Immunology 2024Quote: Cytokines and chemokines from culture supernatants were analyzed using human cytokine MILLIPLEX Luminex (EMD Millipore). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... Downregulation of PAK6 expression was performed utilizing an shRNA against human PAK6 (sh-1944, Sigma). Western blot in SH-SY5Y cell extracts was performed with anti-PAK6 (Abcam ...
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bioRxiv - Developmental Biology 2020Quote: ... equal amounts of lysates (containing 5 mg of total cellular protein from HeLa cells) were pre-cleared with protein G-agarose beads (Sigma, St Louis, MO). Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... and 10-μg protein samples were separated on NuPAGE 4%-12% Bis-Tris protein gels and transferred onto Immobilon-P PVDF membranes (EMD Millipore, Billerica, MA). The membranes were incubated for 1 hour in blocking buffer (Tris-buffered saline with 0.1% Tween and 5% nonfat dry milk ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-HKU1 proteins or lacking the fusion protein (Δ Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland). HAE cultures were washed thrice with 100 μl of 1 × PBS and subsequently inoculated with S-HKU1 or VSV-G pseudoviruses ...
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bioRxiv - Developmental Biology 2020Quote: ... equal amounts of lysate (containing 5 mg of total cellular protein from HeLa cells) were pre-cleared with protein G-agarose beads (Sigma, St Louis, MO). Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche, ...
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bioRxiv - Cancer Biology 2024Quote: PLA was performed to detect protein-protein interactions in MDA-MB-231 cells using Duolink™ In Situ Red Starter Kit (Sigma, cat. DUO92101). All the reagents ...
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bioRxiv - Cell Biology 2023Quote: ... The separation of the hydrophobic and hydrophilic proteins from the pellet was carried out with CelLyticTM MEM Protein Extraction Kit (Sigma-Aldrich, MO, USA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with 20nM rAgApyrase and an unrelated protein amongst the eight candidate proteins (AGAP007393) in carbonate bicarbonate buffer pH 9.6 (Millipore-Sigma, Burlington, MA, USA, #SRE0102). Plates were washed with 0.05% v/v Tween-20 in TBS (25 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: ... Ca2+ transport was terminated at serial time intervals by vacuum filtering 100 µL of assay solution (1 µg of SR protein) through a glass-fiber filter (Millipore HA, with 0.45 µm pore size), which was washed twice with 5 mL of ice-cold 150 mM NaCl solution ...
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bioRxiv - Cancer Biology 2021Quote: ... PDGCLs were lentivirally transduced with the MISSION® shRNA vector pLKO.1-puro-CMV-Turbo green fluorescent protein (TurboGFP)_shnon-target (#SHC016, Sigma, part of Merck, Darmstadt, Germany) for cytosolic TurboGFP expression ...
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bioRxiv - Cell Biology 2024Quote: ... For immunoprecipitation PNS were incubated with Anti-V5 Agarose Affinity Gel conjugated beads or with Protein-A beads (Sigma, 1:10 w/v swollen in PBS) and anti-HA antibody for 2 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Extracellular acidification rate (ECAR) measurements were collected and normalized to protein content that was determined by Pierce™ BCA Protein Assay (Sigma, Catalog number 23228). Glycolysis ...
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with 20nM rAgApyrase and an unrelated protein amongst the eight candidate proteins (AGAP007393) in carbonate bicarbonate buffer pH 9.6 (Millipore-Sigma, Burlington, MA, USA, #SRE0102). Plates were washed with 0.05% v/v Tween-20 in TBS (25 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... The elutes were incubated with S-protein beads (EMD Millipore) overnight at 4 °C ...
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bioRxiv - Biophysics 2021Quote: Protein samples were diluted in PBS pH=7.4 (Sigma-Aldrich). Neutravidin (NAv-Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were alkylated using 2-chloroacetamide (C0267-100G, Sigma-Aldrich) in elution buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: Proteins were concentrated for structural studies using ultrafiltration (Sigma-Millipore, AmiconUltra 4 mL ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysates were incubated with protein G agarose (IP04, Millipore), Flag affinity agaros (A2220 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were reduced with 100 mM DTT (Sigma, D9163) for 35 min at 57°C and then handled according to the FASP (Filter Aided Sample Preparation ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were transferred to an Immobilon-Fl membrane (Millipore). The membrane was after transfer blocked using the Odyssey blocking buffer (Licor ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was transferred to Immobilon-FL PVDF Membrane (Millipore #IPFL00010) in 1 x Tris/Glycine buffer (Biorad #161-0772 ...