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Citations for Millipore Sigma :
4851 - 4900 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... then concentrated using 15 ml Amicon 100 kDa centrifuge filter columns and purified by nickel column affinity chromatography (Novagen His-Bind Resin) as per the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... the nitrocellulose membranes containing the purified recombinant fusion proteins were incubated with mouse anti-His antibody [clone HIS.H8] (Millipore Sigma, Burlington, Massachusetts). HRP-conjugated rabbit anti-mouse IgG antibody (SouthernBiotech ...
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bioRxiv - Developmental Biology 2021Quote: ... One group was injected intraperitoneally (i.p.) with LPS (Escherichia coli serotype 0111:B4; Sigma-Aldrich Canada, Oakville, ON, Canada) on GD13.5 (10 µg/kg ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Cell Biology 2020Quote: ... β- and γ-Syn were produced by co-expressing PT7-7 plasmids with yeast N-acetyltransferase complex B (NatB)74 in Escherichia coli BL21 Star (DE3) cells using M9 minimal media supplemented with 0.5 g/L of 15NH4Cl (Sigma). Protein purification under non-denaturing conditions was performed as described previously75 ...
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bioRxiv - Immunology 2021Quote: ... Phorbol 12-myristate 13-acetate (P8139) and Lipopolysaccharides from Escherichia coli 055:B5 (L2880) were purchased from Sigma-Aldrich. Pam3CSK4 (tlrl-pms) ...
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bioRxiv - Biophysics 2020Quote: ... coli DH10BacY competent cells and plated on LB-Agar plates supplemented with 30 µg ml-1 kanamycin (#K4000, Sigma), 7 µg ml-1 gentamycin (#G1372 ...
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bioRxiv - Biochemistry 2020Quote: ... or catalytically-inactive BT4656S1_11 (in which Ser 77 is intact but prevents formation of formylglycine during expression in E.coli) were expressed in Escherichia coli strain TUNER (Novagen), and cultured to mid-exponential phase in LB supplemented with 50 μg/mL kanamycin at 37 °C and 180 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of LPS from Escherichia coli O111:B4 (sigma) in 0.5% solution of carboxymethyl cellulose (sodium salt; Sigma) was injected into the air pouch ...
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bioRxiv - Plant Biology 2021Quote: ... coli expressing FLAG-tagged RIN4 protein was incubated with 20 µL of anti-FLAG M2 affinity gel (Sigma-Aldrich) at 4°C for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... coli OP50-1 (from the Caenorhabditis Genetics Center, CGC) which was grown in standard LB broth with Streptomycin (Sigma) from a 12.5 mg/ml stock dissolved in H20 ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21 (DE3) was used as the host for expressing recombinant proteins encoded in the pET30a vector (Novagen, USA). Appropriate antibiotics were added when needed at the following concentrations ...
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bioRxiv - Bioengineering 2020Quote: Experiments to confirm the functionality of the vasculature channel and its ability to respond to stimuli were conducted by exposing the epithelial channel of the Open-Top Organ-Chip to 50μl of 10μg/ml Lipopolysaccharide (LPS)(Escherichia coli O111:B4, Sigma L4391) solution for 8 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... coli genes for proteins bS6 and bL9 were PCR-amplified and cloned individually into the pET28(a) plasmid (Novagen). Recombinant single-cysteine proteins were then expressed ...
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bioRxiv - Molecular Biology 2020Quote: ... were treated with 1 μg/ml LPS from Escherichia coli (serotype 0111: B4, Sigma-Aldrich, St. Louis, MO, USA) and 100 ng/ml IFN-γ (ProSpec-Tany Technogene Ltd ...
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bioRxiv - Microbiology 2019Quote: ... or adult control mice (n = 6) received an intra-peritoneal (IP) injection of 1.25mg/kg LPS from Escherichia coli 0111:B4 (Sigma), 0.5mg/kg murine Tumour Necrosis Factor Alpha (TNF-ɑ ...
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bioRxiv - Molecular Biology 2021Quote: Wild-type or mutated pMAL-c2x-malE-L375-his10 plasmids were transformed into Escherichia coli strain BL21 (EMD Millipore) for subsequent growth in LB broth supplemented with 50 μg/ml carbenicillin and 0.2% (w/v ...
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bioRxiv - Immunology 2021Quote: ... LPS (Escherichia coli serotype 0111: B4) and Acid phosphatase leukocyte (TRAP) kit (387A) were procured from Sigma-Aldrich (USA). Macrophage-colony stimulating factor (M-CSF ...
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bioRxiv - Immunology 2022Quote: ... Maturation of iDCs was performed in the presence of 200 ng/ml LPS from Escherichia coli (Sigma Aldrich, Germany) and 10µg/ml myelin basic protein (MBP ...
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bioRxiv - Immunology 2022Quote: ... CTV labeled cells at a concentration of 2×106 cells per mL were stimulated in complete B cell medium supplemented with combinations of 10μg/mL of LPS from Escherichia coli O55:B5 (Sigma) or 1μg/mL CD40L (Peprotech) ...
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bioRxiv - Microbiology 2023Quote: Atypical Enteropathogenic Escherichia coli O125ac:H6 (DSM8711) was purchased from the Leibniz Institute DSMZ and cultured in Luria-Bertani (LB) medium (Merck Millipore) and when necessary supplemented with kanamycin (50 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... coli Tuner (DE3) cells and purified by immobilized metal affinity chromatography (IMAC) using PureProteome™ Nickel Magnetic Beads (Millipore). For a detailed protocol ...
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bioRxiv - Molecular Biology 2023Quote: All PhPINK1 (residues 115–575) constructs and λ-PP were expressed in Escherichia coli Rosetta2 (DE3) pLacI cells (Novagen) and purified as described previously (17) ...
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bioRxiv - Immunology 2023Quote: ... Ultrasound-guided intra-amniotic injection of lipopolysaccharide of Escherichia coli O111:B4 (LPS; Sigma-Aldrich, St. Louis, MO, USA) at a concentration of 100 ng dissolved in 25 µL of sterile 1X PBS was performed into each amniotic sac using a 30 G needle (BD PrecisionGlide Needle) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strains were were cultivated in Lysogeny Broth (Bertani, 1951) medium supplemented with 100 mg/L of ampicillin (Sigma).
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bioRxiv - Neuroscience 2023Quote: ... 3- to 5-month-old male C57Bl/6J received a single intraperitoneal (i.p.) injection of lipopolysaccharide (1.5 x 107 EU/kg) from Escherichia coli 0111:B4 (Sigma, #L2630) or an equivalent volume of sterile saline ...
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bioRxiv - Immunology 2023Quote: ... background were injected intraperitoneally (i.p.) with 35 mg/kg of body weight LPS (lipopolysaccharides from Escherichia coli O111:B4, obtained from Sigma, catalog number ...
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bioRxiv - Immunology 2024Quote: ... cells were cultured in the presence of brefeldin A and LPS (1 μg/ml Escherichia coli O55:B5; Sigma). For intracellular cytokine staining ...
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bioRxiv - Plant Biology 2020Quote: ... 2015) e cleaned using stage-tips preparations with 3 plugs of Empore polystyrenedivinylbenzene (SBD-XC) copolymer disks (Sigma Aldrich, MO, USA) for solid phase extraction following the manufacturers instructions.
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bioRxiv - Developmental Biology 2019Quote: Embryos designated for hematoxylin and eosin (H&E) staining were dissected and fixed overnight in 10% neutral buffered formalin solution (Sigma-Aldrich) at room temperature (RT ...
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bioRxiv - Cancer Biology 2021Quote: ... After 10 days constructs were fixed in 4% paraformaldehyde and embedded in paraffin and stained with H&E and stained for Fibronectin with FN1 antibody (F3648, Sigma Aldrich). For each construct the number of cells invading into the collagen was counted in at least five different fields of view under light microscopy ...
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bioRxiv - Biochemistry 2021Quote: ... 0.01 mg aprotinin and 0.1 mM trans-epoxysuccinyl-L-leucyl amido (4-guanidino) butane (E-64) as a protease inhibitors (Sigma Aldrich®) and lysed by sonication (5 pulses for 1 min each ...
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bioRxiv - Immunology 2021Quote: ... HEK293 and HEK293T cells (gifts from Caetano Reis e Sousa, The Francis Crick Institute, UK) were maintained in DMEM (Sigma-Aldrich) containing 4.5 g/L glucose ...
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bioRxiv - Microbiology 2021Quote: ... PMH were cultured in hepatocyte culture medium (William’s E medium, GlutaMAX™ Supplement, Gibco™ 32551020; 100 U/ml penicillin/streptomycin, Sigma P4333 ...
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bioRxiv - Cancer Biology 2021Quote: ... The retrieved cells were seeded on collagen-treated vessels (at a density of 150,000 viable cells per cm2) and maintained in Williams’ medium E (Sigma, Darmstadt, Germany) supplemented with 1 μM dexamethasone ...
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bioRxiv - Cancer Biology 2021Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then incubated with 1 ml of SF DMEM containing the broad-spectrum hydroxamate metalloproteinases inhibitor CT1746 (100 μM) and the protease inhibitor cocktail (a mixture of aprotinin, bestatin, E-64, leupeptin and pepstatin A)(SIGMA, P1860) in the presence or absence (control ...
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bioRxiv - Biophysics 2022Quote: ... The E protein was cleaved from the EGFP-8xHis fusion by incubation of the beads with 150 units of thrombin (Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... As negative control one set of samples was pre-incubated with 20 μM E-64 (Sigma-Aldrich, St. louis, Mississippi, USA). Subsequently ...
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bioRxiv - Biophysics 2021Quote: ... cell monolayers were allowed to migrate for at least four hours before exchanging the medium with pre-warmed medium containing 10 μg/ml E-cadherin blocking antibody (clone DECMA-1, Millipore MABT26).
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bioRxiv - Microbiology 2019Quote: Peptidoglycan-derived stem peptide fragments including ‘A-E-K-A-A’ pentapeptide (L-Ala-D-Glu-L-Lys-D-Ala-D-Ala (Sigma-Aldrich), ‘K-A-A’ tripeptide (acetyl-L-Lys-D-Ala-D-Ala ...
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bioRxiv - Microbiology 2024Quote: ... and methyl N-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]-N-methoxycarbamate (i. e. pyraclostrobin, pesticide analytical standard, CAS 175013-18-0, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Cell Biology 2023Quote: Cells post aggregation and appropriate control (EGTA for E-cad and Cells without DNA labelling for DNA-cad) were incubated on ice with RIPA lysis buffer (Sigma Aldrich) supplemented with proteases and phosphatases inhibitor cockbTails (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Central transverse sections of the heart were cut into 4 μm sections and stained with haematoxylin and eosin (H&E) and Masson’s Trichrome staining (#HT15, Sigma-Aldrich, UK) for collagen detection ...
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bioRxiv - Immunology 2023Quote: ... Staining was done using hematoxylin and eosin (H&E) to view the alveolar spaces and inflammatory cells and Grocott’s Methenamine Silver stain (GMS) (Sigma-Aldrich, USA) to stain fungal cells in infected tissue ...