Labshake search
Citations for Millipore Sigma :
4751 - 4800 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2019Quote: ... adjusted to 340±5 mOsm with H20) then treated with 20 mg/ml Pronase E in L15/FBS buffer (10% FBS and 1% Penicillin-Streptomycin -Sigma P4458-in L-15 insect medium ...
-
bioRxiv - Biochemistry 2020Quote: ... ii) storage buffer (10 mM NaCl, 10 mM Tris-HCl) with 0.1 mg Pronase E from Streptomyces grisens (Sigma Aldrich) with 0.5% (v/v) ...
-
bioRxiv - Cancer Biology 2019Quote: ... and subsequently treated with 90nM cyst(e)inase in the presence of the following rescuing agents: 100uM Trolox (Sigma, 238813), 50nM ZVAD-FMK (SelleckChem ...
-
bioRxiv - Cell Biology 2020Quote: ... 3d, e, Fig. 4b) or 15kPa gels (Fig. 4g, h) were treated with 15µM of Blebbistatin or DMSO (Sigma-Aldrich) in ENR medium for 3h ...
-
bioRxiv - Cell Biology 2021Quote: ... the imaging media with alpha factor was removed and new media with 0.1 mg/ml Pronase E (Sigma P-6911) was added to inactivate any remaining alpha factor ...
-
bioRxiv - Biochemistry 2022Quote: Purified full-length E-cadherin was concentrated to 8-10 μM and mixed with the nanodisc scaffolding protein MSP1D1 (Sigma) at a 5 fold molar excess ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5 weeks and 8 weeks old C57BL/6J mice were administered with E2 (Cat No. E-2758, Sigma, MO, USA), which was dissolved in ethanol and diluted in corn oil (Cat No ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.25 mg/ml Pepstatin A and 0.25 mg/ml E-64) or Roche cOmpleteTM EDTA-free protease inhibitor cocktail tablet (Millipore-Sigma). NP-40 (final concentration of 0.5% ...
-
bioRxiv - Neuroscience 2021Quote: ... or glass coverslips in 24-well plates (for immunocytochemistry) coated with Poly-D-Lysine (5 μg/ml, PDL, A-003-E; Millipore) and laminin (1 mg/ml Sigma L2020) ...
-
bioRxiv - Genetics 2019Quote: ... Cells were released from G1 by washing with YPD containing auxin (if indicated) and 0.15mg/mL pronase E (Sigma Aldrich) three times ...
-
bioRxiv - Microbiology 2021Quote: ... was incubated in 50 mM Tris-acetate buffer (pH 7.4) containing 5 mg/mL protease mixture (Pronase E, Sigma-Aldrich) in a total volume of 1 mL at 28 °C for 2 h and then directly resuspended in the standard SDS sample buffer before SDS-PAGE and western blot analysis.
-
bioRxiv - Biochemistry 2021Quote: ... 0.3 ml of media were precipitated with 25 µl EZ view Red anti-HA affinity gel (E 6779; Sigma-Aldrich) according to the manufacturers’ protocol ...
-
bioRxiv - Immunology 2019Quote: ... pneumoniae in Colombia blood agar (E&O Laboratories, UK) followed by Todd-Hewitt broth plus 0·5% yeast extract (Sigma), both at 37°C in 5% CO2 ...
-
Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-SeqbioRxiv - Developmental Biology 2021Quote: ... animal cap - (E) vegetal cap. Total RNA (X. laevis and A. mexicanum) was extracted using TriReagent extraction and LiCl precipitation (Sigma) (details in (Sindelka et al ...
-
bioRxiv - Cell Biology 2022Quote: ... 80-mer oligonucleotides biotinylated either on the 5’ or 3’ end containing the E-box of the Lgr5 promoter or a mutated E-box (see table) were annealed with the corresponding antisense sequence and bound to streptavidin agarose beads (S1638, Millipore). Beads were washed 3 times ...
-
bioRxiv - Neuroscience 2022Quote: ... and placed in a T75 flask (pre-coated with 50 μg/mL poly-D-lysine (Merck Millipore, A-003-E) for 1 hour) ...
-
bioRxiv - Genetics 2024Quote: ... in TC-treated 12- or 6well plates coated with 0.05 mg/mL Poly-D-lysine (Sigma-Aldrich A-003-E). At 3-4 DIV ...
-
bioRxiv - Immunology 2023Quote: ... HEK293FT (provided by T. Brdicka, IMG) and MC-38 cells (provided by E. Palmer, University Hospital Basel) were cultivated in DMEM media (Sigma). The media were supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2023Quote: DO11.10 cell line (provided by Tomas Brdicka, IMG) and T2–Kb (provided by E. Palmer, University Hospital Basel) were cultivated in RPMI media (Sigma). HEK293FT (provided by T ...
-
bioRxiv - Neuroscience 2023Quote: ... and the gamma secretases inhibitor was purchased as Compound E (γ-Secretase Inhibitor XXI) from EMD Millipore (Burlington, MA, USA). Ammonium acetate (Cat# A11450 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Slides were stained with hematoxylin and eosin (H&E) and for Tph1 with an anti-Tph1 primary antibody (Sigma; #T0678). Detection was performed using the polymer-based Envision FLEX+ detection system (Agilent).
-
bioRxiv - Bioengineering 2023Quote: ... and 5 μm sections were taken before being baked for 3 hours at 60°C and stained with hematoxylin and eosin (H&E, Sigma) or Masson’s trichrome (Sigma ...
-
bioRxiv - Bioengineering 2022Quote: ... and tumor target (T) cells were co-cultured in triplicates at the indicated E/T ratio using flat bottom poly-L-ornithine (Sigma) coated 96-well plates with 1 × 104 target cells in a total volume of 200 µL per well in NALM-6 medium ...
-
bioRxiv - Cell Biology 2023Quote: Transient knockdown of S100A11 and E-cadherin in MCF-10A cells was performed by transfection of MISSION® Predesigned (Sigma) or custom-made siRNAs ...
-
bioRxiv - Bioengineering 2023Quote: ... samples were mounted on paraffin blocks and sectioned into 5 µm sections then stained with hematoxylin and eosin (H&E, Sigma) or picrosirius red (PSR ...
-
bioRxiv - Neuroscience 2023Quote: ... brain homogenate from a RML inoculated C57BL6/J mouse at the terminal phase of disease was treated with 100 μg/ml of pronase E (Sigma) for 30 min at 37 °C with shaking in a thermal mixer at 800 rpm ...
-
bioRxiv - Neuroscience 2024Quote: ... The first injection of pre-treatments was a solution of ethosuximide (150 mg/kg of 84 mg/ml in phosphate buffered saline, s.c.; Cat# E;7138, Sigma-Aldrich). Ethosuximide was used because the background strain ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and MRS 1220 (N-(9-chloro-2-furan-2-yl-[1,2,4]triazolo[1,5-c]quinazolin-5-yl)-2-phenylacetamide) were purchased from Sigma-Aldrich and dissolved in dimethyl-sulphoxide (DMSO) ...
-
bioRxiv - Bioengineering 2022Quote: ... and 1% w/v of 2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure D-2959) photoinitiator (Sigma-Aldrich) in phosphate buffered saline (PBS ...
-
bioRxiv - Cancer Biology 2022Quote: The peptides mUNO (sequence: CSPGAK-COOH) and FAM-mUNO (FAM-Ahx-CSPGAK-COOH) were purchased from TAG Copenhagen and doxorubicin (DOX) from Sigma-Aldrich. St-PGA was kindly provided by Polypeptide Therapeutic Solution S.L ...
-
bioRxiv - Synthetic Biology 2019Quote: ... the 6x-His Tag Polyclonal Antibody or HA Tag Polyclonal Antibody was the primary antibody and the anti-rabbit IgG-gold (10 nm) antibody (Sigma-Aldrich) was the secondary ...
-
bioRxiv - Cell Biology 2021Quote: ... The gDNA extractions and PCR verifying integration of the fluorescent tags into the genomic loci was performed using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR amplicons were run on 1 or 2% agarose gels in TBE and gels were imaged with Bio-Rad Gel ChemiDoc XRS system and Quantity One software.
-
bioRxiv - Biochemistry 2020Quote: ... Western blot analysis was used to verify the identity of PfAdoMetDC using monoclonal α-Strep-tag II antibodies (Novagen, Wisconsin, USA). Following Strep-Tactin Sepharose affinity column purification ...
-
bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
-
bioRxiv - Cell Biology 2022Quote: ... It was inserted with N and C-terminal tags (His6 and StrepII, respectively) in the baculovirus transfer vector pIEX/Bac3 (Merck Novagen #717263).
-
bioRxiv - Cell Biology 2021Quote: ... the supernatants obtained were incubated with gentle rotation for 1 h at 4°C with anti-FLAG tag-antibody-conjugated magnetic beads (Sigma-Aldrich). The beads were then washed three times with lysis buffer #1 and incubated with gentle rotation for 30 min at 4°C with 50 μL of an elution buffer (50 mM HEPES-KOH ...
-
bioRxiv - Microbiology 2021Quote: ... The proteins were eluted using 250 µg/mL Flag peptide into wash buffer for 1 h at 4°C and subjected to immunoblotting analysis using antibodies for Flag tag (SIGMA, SLCD6338) and SARS-CoV-2 S (Sino Biological ...
-
bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
-
bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
-
bioRxiv - Immunology 2022Quote: ... followed by blocking for 1 h with 5% nonfat milk in Tris-buffered saline-Tween 20 buffer and probing with antibodies against Strep Tag (SAB2702215, Sigma-Aldrich), γ-catenin (sc-514115 ...
-
bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with protein A/G agarose beads (Pierce, Waltham, MA, USA) conjugated to antibodies against FLAG-tag (Sigma-Aldrich) or Exo70 (Santa Cruz ...
-
bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663 ...
-
bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were separated by SDS-PAGE on a 4-20% gradient gel and then immunoblotted as described above using antibodies directed against the GST tag (Sigma-Aldrich) to detect CBUA0027 ...
-
bioRxiv - Bioengineering 2022Quote: ... samples were washed 3× five minutes in BSA-T and incubated with an antibody against the FLAG-tag (#F1804 Sigma-Aldrich, now Merck ...
-
bioRxiv - Cell Biology 2022Quote: ... oligos carrying the ALFA tag sequence codon optimized for yeast and flanked by SalI and BglII sites were synthesized by Sigma-Aldrich. These oligos were annealed in annealing buffer (10 mM Tris pH 7.5-8.0 ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were separated by SDS-PAGE on a 4-20% gradient gel and then immunoblotted as described above using antibodies directed against the GST tag (EMD Millipore) to detect CBUA0027 ...
-
bioRxiv - Biochemistry 2024Quote: ... numbering includes the signal peptide) with C-terminal Flag and 6x-Histidine tags and subsequently cloned into a p32A vector (Millipore Sigma). The TrxA-3C-ligand-Flag-6xHis construct was transformed into Origami E ...
-
bioRxiv - Microbiology 2023Quote: ... gBlock contained vector-specific sequence) methods into pET28a (cytochromes, NdeI/XhoI sites, in frame with N-terminal His6 tag, Novagen 69864) or pGEX (HCCS/HCC1S ...
-
bioRxiv - Bioengineering 2023Quote: A 10 mg/mL suspension of TAG drops was prepared by dissolving 1 mg of 1,2-dioleoyl-3-palmitoyl-rac-glycerol (Sigma-Aldrich Inc.) in 100 μL of a chloroform–methanol solution (2:1) ...