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Citations for Millipore Sigma :
4851 - 4900 of 5860 citations for QuantiChrom Hemoglobin Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The HEK cells used in this study were found free of mycoplasma infection (Sigma-Aldrich mycoplasma detection kit). These cells were cotransfected with 0.1 - 1 μg of mutant hHV1-TAG plasmid and 0.7 μg of pAnap plasmid (a gift from Dr ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from cells using the GenElute™ Mammalian Total RNA Miniprep Kit (Sigma-Aldrich, RTN350). cDNA was reversely transcribed from 1 µg of RNA using the iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... iNs were subjected to in situ PLA using Duolink In Situ Detection Reagents Red kit (Sigma, catalog #: DUO92008) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: The intracellular ROS levels in C6 cells were determined using the Fluorometric Intracellular ROS Kit (MAK142; Sigma, USA), which was performed in a 96-well plate format with detection at an excitation/emission wavelengths of 650/675 nm 29.
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bioRxiv - Cell Biology 2022Quote: ... allowing for detection of the secondary HRP antibody detected using a chemiluminescence peroxidase substrate kit (Sigma, #CPS-1) and subsequent detection of the GAPDH loading control detected using its laser based infrared detection system ...
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bioRxiv - Cancer Biology 2023Quote: ... Mycoplasma testing was routinely performed using the LookOut® Mycoplasma PCR Detection Kit (Sigma, MA, USA, MP0035-1KT) to ensure the absence of contamination before cells were used in experiments.
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bioRxiv - Plant Biology 2023Quote: ... RNA from young kiwifruit tissue culture leaves was extracted using the Spectrum Plant Total RNA kit (Sigma-Aldrich), following the manufacturer’s protocol except the incubation temperature of lysed tissue sample was increased to 65°C.
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bioRxiv - Neuroscience 2023Quote: ... TUNEL-positive cells were stained using the in-situ cell death detection kit TMR red (11684795910; Sigma-Aldrich) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Please note that most of the solutions listed are contained within the Duolink® PLA Kit (Sigma DUO92008). Upon receiving the PLA kit (Sigma DUO92008) ...
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bioRxiv - Microbiology 2023Quote: ... stained with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane (Sigma-Aldrich, Saint Louis, Missouri, USA) according to manufacturer’s instructions and added at a concentration of 5×105 monocytes per model the day before adding epithelial cells.
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bioRxiv - Microbiology 2023Quote: ... under the control of the IPTG inducible T7 promoter using the pET-30 Ek/LIC Cloning Kit (Novagen). His/Strep-GlnA3Mt was synthesized in E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Further extraction was done using RNA the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich Chemie GmbH, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA was extracted from ear slices with the GenElute Mammalian Genomic DNA Mini-prep Kit (Sigma-Aldrich). PCR was performed with PrimeSTAR® DNA Polymerase (Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... an additional step of dialysis using Pur-A-LyzerTM Mini 6000 dialysis kit (PURN60030, Sigma-Aldrich, MO, USA) was added to remove iodixanol before grid preparation ...
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bioRxiv - Biochemistry 2023Quote: ATPase activity of FtsEX-EnvC was measured using a malachite green based colorimetric ATPase kit (MAK307, Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were screened negative for mycoplasma contamination by monthly scheduled testing (LookOut® Mycoplasma PCR Detection Kit, Sigma). Stable cell lines expressing Sm.TRPMPZQ or Fh.TRPMPZQ were generated using the Flp-In T-REX core kit (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Offspring were screened for Taf7l mutations using REDExtract-N-AmpTM Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA) extracted genomic DNA from tail-tip biopsies ...
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were fixed in 4% PFA for 2 min and stained with Alkaline Phosphatase Detection Kit (Sigma-Aldrich). Colonies were manually counted distinguishing between differentiated ...
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bioRxiv - Molecular Biology 2024Quote: ChIRP was performed in HeLa cells by using the Magna ChIRPTM RNA Interactome Kit (Sigma, cat #17-10494) strictly following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cytoskeleton fraction characterization was performed using the ProteoExtract® Cytoskeleton Enrichment and Isolation Kit (Sigma-Aldrich, 17-10195) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... Cells were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Approximately 100mg of tissue was used for RNA extraction using Spectrum Plant Total RNA Kit (Sigma-Aldrich, USA) according to the manufacturer instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... toruloides strains cultured in the nitrogen-limited medium using the Spectrum Total RNA Kit (Sigma-Aldrich, Oakville, Canada). First-strand cDNA was synthesized with the SuperScript IV first-strand cDNA synthesis kit (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Triglyceride and free fatty acid levels were assessed using colorimetric kits (Cayman Chemicals #10010303 and Sigma-Aldrich #MAK044), respectively.
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bioRxiv - Microbiology 2024Quote: ... We confirmed the absence of mycoplasma contamination in cells using the LookOut Mycoplasma PCR Detection Kit (Millipore Sigma).
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bioRxiv - Neuroscience 2024Quote: ... antibodies were conjugated to plus-and minus-oligonucleotides using the Duolink PLA Probemaker kits (Sigma, #DUO92009 and #DUO92010) according to manufacturer’s proto-col ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 50 µL of eluate was measured with an colorimetric iron detection kit (Sigma-Aldrich, Cat. No. MAK025-1KT) following the provided protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... 14µm sections made with a Leica CM3050s cryostat were stained using a Masson Trichrome Staining Kit (Sigma-Aldrich). Manufacturer’s protocol was followed except for the omission of deparaffinization and microwaving ...
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bioRxiv - Physiology 2024Quote: ... Cell lines were tested at least twice a year for Mycoplasma contamination using the LookOut Detection Kit (Sigma), and all tests were negative.
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bioRxiv - Cancer Biology 2021Quote: ... Counterstaining of nucleus was performed using Mayer’s hemalum solution and slides were mounted with Kaiser’s glycerol gelatin (the Kit and all reagents were obtained from Merck Millipore). Images were acquired as described above.
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bioRxiv - Cell Biology 2021Quote: Apoptotic cells were visualized with the terminal deoxynucleotidyl transferase (TdT)-FragEL DNA Fragmentation Detection Kit (Millipore, Etobicoke, ON, Canada) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: Cells were lysed with 0.5% SDS in EB buffer and bisulphite treated with the Imprint DNA Modification kit (Sigma). The resulting DNA was purified using columns and reagents from the EZ DNA Methylation Direct kit (Zymo Research) ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was blocked for 1 hour in 1% BSA/TBS-T and probed with antibodies provided in the kit according to the manufacturer’s instructions (Millipore).
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bioRxiv - Molecular Biology 2021Quote: ... 1/1000) antibodies for 2 h at 37 °C in antibody diluent solution (Duolink in situ kit, Sigma-Aldrich). The cells were then labeled with the PLA antimouse PLUS probe (DUO92001 ...
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bioRxiv - Molecular Biology 2021Quote: RNA extraction with on-column DNAse treatment were done using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated from the aboveground tissues of seedlings using the Spectrum Plant RNA Isolation Kit (Sigma-Aldrich). The RNA was treated with RNase-free RQ1 DNase (Promega) ...
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bioRxiv - Cancer Biology 2022Quote: ... Ligation and amplification steps of the PLA were performed using the Duolink in situ Detection Reagents Orange kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and performed western blotting analysis.Western blotting: Protein concentration of lysates was determined with Bicinchoninic Acid Kit (#BCA1, Sigma Aldrich). Equal amount of proteins was loaded on the poly-acrylamide gels ...
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bioRxiv - Molecular Biology 2020Quote: Yolk sacs were dissected from embryos and used for DNA extraction with the Red Extract-N-Amp kit (Sigma). Usp9x status was assessed by PCR using Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... PLA and EdU counterstaining were performed according to the manufacturer’s instructions using the Duolink In Situ Red kit (Sigma) and goat anti-mouse Alexa Fluor 488 antibody.
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bioRxiv - Evolutionary Biology 2022Quote: ... We extracted DNA from our cultured microbiomes with GenElute Bacterial Genomic DNA kits (Millipore-Sigma, St. Louis, MO, USA). We sent ≈10 ng of DNA from each extraction to Genome Qúebec (McGill University ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We extracted DNA from our cultured microbiomes with GenElute Bacterial Genomic DNA kits (Millipore-Sigma, St. Louis, MO, USA). We sent ≈10 ng of DNA from each extraction to Genome Qúebec (McGill University ...
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bioRxiv - Molecular Biology 2019Quote: Whole-tissue sample lysates were batch processed for glycogen determination using commercially-available fluorometric kits (catalog #: MAK016; Millipore Sigma) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... gDNA for illumina sequencing of the eleven other isolates was extracted using a Genelute Plant DNA Miniprep Kit (Sigma) using the manufacturers protocol with the following modifications ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from flower buds or leaves with the Spectrum Plant Total RNA kit (Sigma, Inc., USA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The protein band was excised from gel and digested with trypsin using trypsin profile IGD kit (Sigma-Aldrich, India). The peptides obtained after trypsin digestion were analyzed by Matrix-assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF ...
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bioRxiv - Plant Biology 2020Quote: ... France) and 500 μl of lysis/2-Mercaptoethanol solution of the SpectrumTM Plant Total μ RNA Kit (Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were subsequently fixed in 8% formaldehyde and stained using the Alkaline-Phosphatase kit (86R 1KT, Sigma-Aldrich, Germany) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... cells were collected at day 8 and RNA was extracted using GenElute Mammalian Total RNA MiniPrep Kit (Sigma #RTN350). cDNA was synthesized using oligodT primers using SuperScript II Reverse Transcriptase (Thermo Fisher Scientific #18064014) ...