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Citations for Millipore Sigma :
4801 - 4850 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... transferred onto nitrocellulose membranes and incubated overnight at +4 with primary antibodies against PROX1 (goat-anti-human, AF2727, R&D, 1:1000) and vinculin (mouse anti-vinculin, V9131, Sigma, 1:1000). HRP conjugated secondary antibodies (Dako ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by 1 h incubation at RT in secondary antibodies (Anti-Rabbit: A0545; Anti-Mouse: A6782, Sigma-Aldrich Quimica S.A., Madrid, Spain) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated overnight at 4°C with primary antibodies (anti-β III tubulin mouse monoclonal, Sigma, T7816 and anti-DCX, Guinea Pig polyclonal, Millipore, AB5910) at dilution of 1:1000 in 1% BSA in PBST (0.5% triton added to PBS) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for immunostaining include rabbit anti-human/mouse PKD-1 (4502371) and mouse anti-mouse α-smooth muscle actin(A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated in rabbit anti-Iba1 (1:1000, Wako, Cat.#019-19741) and chicken anti-GFAP antibody (1:2000, Sigma, Cat#AB5541) dissolved in PBST at 4 °C for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10,000; anti-rabbit, Sigma-Aldrich A0545 or anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then incubated overnight at 4°C in a 1:100 dilution of primary antibody (anti-MPP10, Novus Biologicals, NBP1-84341; anti-Fibrillarin, EMD Millipore, MABE1154 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The custom recombinant antibody-like anti-poly-ADP-ribose binding reagent (anti-PAR) was generated and purified in-house (now available from EMD Millipore, MABE1031). The other antibodies used were as follows ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated with one or more of the following primary antibodies (rabbit anti-TH, 1:2000, AB152, EMD Millipore; guinea pig anti-VGLUT2, AB2251, EMD Millipore; chicken anti-GFP ...
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bioRxiv - Neuroscience 2022Quote: ... or 1:100 anti-BKbeta4 K+ channel (Antibodies Inc 75-086) and after striping a mix of anti-γ-tubulin 1:10,000 (Sigma-Aldrich,T5326,) and anti-PSD95 1:5,000 (Antibodies Inc 75-028) ...
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bioRxiv - Developmental Biology 2020Quote: ... washed with TBS-T and incubated overnight with primary antibodies (anti-Actin 1:10000 Santa Cruz Biotechnology #sc1616; anti-Dab1 1:2000 Sigma-Aldrich #HPA052033 ...
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bioRxiv - Microbiology 2020Quote: ... and then incubated overnight at 4 °C with anti-hACE2 antibody (1:1000; R&D AF933) and anti-beta actin (1:2000; CST 4970) in 5% BSA (Sigma-Aldrich A9647) in TBST ...
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bioRxiv - Systems Biology 2020Quote: ... Blots were incubated with appropriate secondary antibodies (Anti-Rabbit IgG-Peroxidase Sigma-Aldrich A0545 and Anti-Mouse IgG-Peroxidase Sigma-Aldrich A9044) and imaged using GE Amersham Imager 600.
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bioRxiv - Plant Biology 2021Quote: ... The glutathionylated VirE2 was detected by the anti-glutathione monoclonal primary antibody (My biosource, 1:1000) and anti-mouse (Sigma, 1:1000) secondary antibody (Klaus et al ...
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bioRxiv - Cell Biology 2021Quote: ... The sections were then stained overnight at 4°C in the respective primary antibodies (anti-Rhodopsin, MAB5316 Sigma-Aldrich; anti-M-Opsin, AB5405 Sigma-Aldrich; anti-S-Opsin ...
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bioRxiv - Neuroscience 2020Quote: Immunostaining for ChAT or TH was performed using the following antibodies: rabbit anti-TH 1:200 (Pel-Freez cat # P4010-0) or goat anti-ChAT 1:25 (Millipore cat # AB144P). Sections were fixed in an acetone/methanol mixture (1:1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein detection was achieved using the following antibodies: anti-Toddler (rabbit, 1:500, provided by the Reversade Lab11) and anti-tubulin (mouse, 1:10000, Sigma-Aldrich T6074).
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bioRxiv - Neuroscience 2022Quote: ... Then membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase conjugated secondary antibodies (1:3000, Sigma Aldrich, St. Louis, MO) for 1 hr ...
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bioRxiv - Bioengineering 2022Quote: ... we used 4% agarose micro-beads bound to the Anti-FLAG M2 monoclonal antibody (Anti-FLAG® M2 Magnetic Beads from Sigma, M8823). Beads size ranges between 20-75 μm ...
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bioRxiv - Neuroscience 2023Quote: ... and stained according to the immunohistochemistry protocol above (primary antibodies: anti-ZnT3, rabbit polyclonal, 1:500, Synaptic Systems; anti-puromycin 1:1000, EMD Millipore) and imaged on the Zeiss LSM 880 with Airyscan using a LD LCI Plan-Apochromat 25x/0.8 mm Korr DIC M27 and 1.8x zoom ...
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bioRxiv - Genetics 2024Quote: ... and secondary antibodies (anti-mouse HRP [1:2,000; Thermo Fisher Scientific] for TARS1 and anti-rabbit HRP [1:5,000; EMD Millipore] for actin) were applied in 2% milk solution ...
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bioRxiv - Cell Biology 2024Quote: ... separated by SDS PAGE and immunoblotted with either anti-phospho-PKA substrate antibody (Cell Siginaling Technology, # 9621) or anti-Tns3 (Millipore Sigma, HPA055338).Ten µg of whole cell extract proteins were used for lysate controls ...
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bioRxiv - Cell Biology 2023Quote: The custom recombinant antibody-like anti-MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076) (Gibson et al. ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-αSMA (Cat: A5228) and mouse monoclonal anti-β-actin (AC-74) (A2228) antibodies were from Sigma (St Louis, MO). Anti-TGFβR1 (Cat ...
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bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse or anti-rabbit, at dilution 1:10000) and Immobilon® Western Chemiluminescent HRP substrate (Merk Millipore, Sigma Aldrich) were used to visualize FLAG-FlaA/FlaB and untagged FlaA/FlaB.
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (anti-mouse-TH; MAB-318, 1:1000; Millipore, Temecula, CA). The sections were then incubated for 1 h with the appropriate secondary antibody after which sections were washed with PBS and incubated with the required detection kits for development ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated for 48-72 hours with the following primary antibodies at 1:400: rabbit anti-Iba1 (Wako, 019-19741) and mouse anti-PSD-95 (Sigma-Aldrich, MAB1598). Sections were then washed 3x with PBS containing 0.05% Tween 20 and incubated with Alexa Fluorophore conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with secondary antibodies conjugated with PLA probes MINUS and PLUS: the PLA probe anti-mouse PLUS and anti-rabbit MINUS (Duolink®, Sigma-Aldrich). The incubation with all antibodies was accomplished in a humidified chamber for 1 h at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used in Western blot were (listed in Appendix Table S4): mouse monoclonal antibodies anti-β-actin and anti-UVRAG (both from Sigma-Aldrich), anti-LC3B (NanoTools) ...
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bioRxiv - Cancer Biology 2024Quote: ... The primary antibody solution was prepared with a dilution of 1:100 of both rabbit anti-human anti-ZO-1 (Sigma-Aldrich, AB2272) and mouse anti-human anti-Vimentin (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the HA-fusion proteins were detected on the same blot with anti-HA11 monoclonal primary antibody (Covance, New Jersey, USA, order number: MMS-101P, diluted 1:1000) and anti-mouse-HRP secondary antibody (Sigma-Aldrich, diluted 1:10000).
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bioRxiv - Biochemistry 2020Quote: ... Ribosomal protein L3 was detected using anti-L3 primary antibodies (a gift from Fujio Kawamura; 1:20,000 dilution) combined with goat anti-rabbit IgG-HRP secondary antibodies (Sigma-Aldrich, A0545; 1:10,000 dilution). ECL detection was performed using WesternBright™ Quantum (K-12042-D10 ...
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bioRxiv - Biochemistry 2021Quote: ... or with anti-GFP primary antibody (1:10000; CHROMTEK 3H9-100 Lot#60706001AB) and then anti-rat-IgG-HRP secondary antibody (1:10000; SIGMA A9037-1ML Lot#SLCF6775). Ponceau staining was used as a loading control.
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bioRxiv - Microbiology 2020Quote: ... Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody (produced in goat) and Anti-Rabbit IgG (whole molecule)–Peroxidase antibody (produced in goat) were received from Sigma-Aldrich (St. Louis, USA). Rabbit anti-human IgG–HRP was procured from GeNei™ ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody (produced in goat) and Anti-Rabbit IgG (whole molecule)–Peroxidase antibody (produced in goat) was received from Sigma-Aldrich (St. Louis, USA). Rabbit anti-human IgG–HRP was procured from GeNei™ ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were loaded on an 8% Tris/glycine gel and subjected to Western blot analysis using different primary antibodies (anti-c-myc (A-14, sc-769, Santa Cruz, Nunningen, Switzerland and HA antibody, rat, 3F10, Sigma-Aldrich, Munich, Germany).
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked with 5% BSA in 1X PBS containing 0.1% Tween-20 (PBT) at RT for 1 hour and then incubated with primary antibody solution containing anti-chondroitin sulfate antibody (Millipore, monoclonal, mouse, 1:100) and biotinylated-Hyaluronan Binding Protein (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... and FITC-labeled anti-FcγRIIb antibody (Cat: NBP2-14905, Biotechne; Cat: MX488AS100-1KT, Sigma-Aldrich) were added into cells (1×106 cells/sample in 100 μL ...
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bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
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bioRxiv - Microbiology 2020Quote: ... 0.1% SDS) and the supernatant was subjected to affinity purification with anti-HA antibody (Sigma) conjugated to magnetics beads ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were incubated overnight with an anti-FLAG-HRP antibody (A8692, dilution 1:1000, Sigma). Antibodies were diluted in TBS-Tween (0.05% ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the primary antibody (monoclonal mouse anti-vinculin, 1:100, Sigma V9264 ...
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bioRxiv - Biophysics 2019Quote: ... antibodies were used in combination with peroxidase-labeled goat anti-mouse IgG (A4416, Sigma-Aldrich). The reactivity was detected with an Amer-sham ECL Detection Reagent (GE Healthcare Life Science ...
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bioRxiv - Neuroscience 2021Quote: ... anti-NeuN antibody conjugated to Alexa Fluor 488 (cat no. MAB377X, EMD Millipore, Burlington MA), anti-PU.1 antibody conjugated to Alexa 647 (cat no ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used at a 1:500 concentration: anti-K48 ubiquitin (AB_11213655, Millipore), anti-HA (AB_390919 ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary Abs were anti-mouse/rabbit IgG peroxidase antibody produced in goat (A0168/A6667; Sigma); Alexa Fluor 488 goat anti-mouse/rabbit IgG (A11029/A11034 ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-AGE (Advanced Glycated End-products) Antibody goat polyclonal 1:200 (Millipore Cat. No. AB9890) Beta-actin loading control 1:400 (Thermo fisher cat no ...