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Citations for Millipore Sigma :
4701 - 4750 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody used was a mouse monoclonal anti-NeuN (1:1000; Millipore MAB377), and the secondary antibody used was a goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... and goat anti-guinea pig secondary antibody conjugated with HRP (1:5000, Sigma, A7289) was added to the wells ...
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bioRxiv - Microbiology 2024Quote: The following antibodies were used for immunoblotting: mouse anti-Flag (Sigma, M2, 1:5,000), rabbit anti-Flag (ProteinTech ...
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bioRxiv - Microbiology 2024Quote: ... FaRel2 was detected using anti-FLAG M2 antibody (Sigma-Aldrich, F1804; 1:10,000 dilution) primary ...
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bioRxiv - Systems Biology 2024Quote: ... and with a guinea pig anti-glucagon primary antibody (Sigma-Aldrich, #G2654; RRID: AB_259852) and goat anti-guinea pig Alexa Fluor 594 secondary (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse anti-PSD-95 antibodies (1:100,000) (#05-494) were purchased from Millipore (Upstate). Donkey anti-rabbit horseradish peroxidase (HRP) ...
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bioRxiv - Developmental Biology 2024Quote: ... using the following primary antibodies: anti-flag HRP-conjugated (1:1000; Sigma-Aldrich, A8592), anti-αTubulin (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... followed by a secondary HRP-conjugated donkey anti-chicken antibody (1:1000) (Merck Millipore). The western blots were analysed with Clarity Western ECL substrate (BioRad ...
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bioRxiv - Genomics 2024Quote: ... diluted 1:750 or with the anti-CENP-B 07-735 antibody (Sigma-Aldrich), diluted 1:1000 ...
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bioRxiv - Plant Biology 2019Quote: ... pulled-down proteins were separated on SDS-PAGE and analyzed by immunoblotting with anti-Flag and anti-Myc antibodies (Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: Cells were stained with rabbit anti-GlActin antibody 28PB+1 (Paredez et al., 2011) and mouse monoclonal anti-HA (Clone HA7, Sigma-Aldrich) both diluted 1:125 in PMEBALG and incubated over-night ...
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bioRxiv - Systems Biology 2021Quote: Spleen cells were seeded into 96-well flat-bottom plates at 5 ×105 cells/well in 100 μl of complete RPMI medium and stimulated with 2 μg/ml of anti-CD3/anti-CD28 antibodies (Sigma-Aldrich) at 37°C and 5% CO ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibodies goat anti-Olig2 (1:100, R&D AF2418) and rabbit anti-NeuN (1:100, Millipore ABN78) for two days at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated overnight at 4 °C in vehicle with primary antibodies (mouse anti-Syt7, 1:200, Neuromab N275/14; rabbit anti-TH, 1:500, Millipore AB152). After 3 washes for 30 minutes in vehicle ...
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bioRxiv - Cancer Biology 2019Quote: ... The RBM proteins were identified with the primary rabbit anti-RBM10 or RBM5 antibody and the following secondary anti-rabbit IgG conjugated with Horse radish peroxidase (A6154; Sigma-Aldrich) or conjugated with alkaline phasphatase (T2180;Applied Biosystems).
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated with HRP-coupled secondary antibodies diluted 1:10,000 in BS (anti-mouse IgG Jackson 515-035-062, anti-rabbit IgG Sigma A-9169), washed twice in BS and twice in TBS ...
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bioRxiv - Biochemistry 2021Quote: ... Bound proteins were eluted by addition of NuPAGE LDS gel loading buffer and proteins in the supernatant and pellet were analysed by gel electrophoresis followed by western blotting using the following antibodies: anti-HOIP (#SAB2102031) and anti-HOIL-1 (#HPA024185) were from Sigma-Aldrich; anti-MBP (#E8032S ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour before incubation with primary antibodies: rabbit anti-NogoA (1:200; Supplementary Table 4) and mouse anti-GFAP (1:500; Sigma-Aldrich), for 72 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... affinity-purified rabbit anti-panx1b antibody, code#ZOI-B2, Davids Biotechnologie GmbH; 1:100, rabbit anti-GFAP, code AB5804, Sigma-Aldrich) overnight at 4℃ ...
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bioRxiv - Neuroscience 2021Quote: ... 1:500 dilution of Rat polyclonal anti-GnRH antibody (Skrapits et al., 2015) and 1:250 dilution Guinea Pig polycloncal anti-NeuN (EMD Millipore). After the brain slices were washed 3x with 1XPBS they were incubated with the following secondary antibodies in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Physiology 2021Quote: ... Protein detection was performed by the use of the HRP-conjugated secondary antibodies: anti-rabbit (CST #7074) or anti-mouse (CST #7076) and Immobilon Forte Western HRP substrate (Millipore Sigma). Protein band intensity on exposed film was measured with Adobe Photoshop software.
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bioRxiv - Microbiology 2020Quote: ... One hundred microliters of secondary antibody (alkaline phosphatase-conjugated goat anti-rabbit IgG or goat anti-human IgG, both from Sigma-Aldrich) diluted 1:2,000 in 1% nonfat milk-PBS were then added to each well and the plates incubated for additional 3 h at room temperature before repeating the washing step ...
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bioRxiv - Microbiology 2020Quote: ... were used with secondary anti-mouse IgG or anti-rabbit IgG conjugated with horseradish peroxidase antibodies (A9917 and AP132P, Sigma Aldrich). SuperSignal West Pico Chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... They were next incubated in primary antibody solution for 2 hours at room temperature (1:1000 anti-Strep, IBA Life Sciences, 1:10,000 anti-FLAG M2 monoclonal antibody, Sigma in 2% milk Tris – buffered saline ...
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bioRxiv - Microbiology 2021Quote: ... The cells were lysed in 5X Laemmli buffer and the lysates were subjected to SDS-PAGE followed by immunoblotting as previously described using an 1/10000 diluted anti-TEM rabbit serum and an 1/10000 diluted anti-actin antibodies (Sigma-Aldricht).
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bioRxiv - Neuroscience 2020Quote: ... and incubated with primary antibodies (rabbit anti-UCP1, 1:750, Abcam ab10983, mouse anti-tyrosine hydroxylase, 1:1,500, Sigma-Aldrich T1299) in blocking buffer overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10000; anti-rabbit, Sigma-Aldrich A0545; anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10000; anti-rabbit, Sigma-Aldrich A0545; anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Biochemistry 2022Quote: ... and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804; rabbit anti-NF-145 – Millipore AB1987), followed by washing in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal antibody against Tamr1 (mAb 1D11), anti-Ta-p104 (Clone IC12, polyclonal mouse antiserum) and rabbit monoclonal anti-acetyl H4 (Sigma-Aldrich). Antibodies were diluted in PBS-1% BSA-0.3% Triton X100 (PBST) ...
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bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530; Merck Millipore) in blocking solution ...
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bioRxiv - Molecular Biology 2020Quote: ... The same steps were carried out after that with the anti-DNA solution (1:50 dilution of the anti-DNA antibody [Millipore, MAB3034]) and the secondary antibodies solution (1:75 dilutions of Alexa Fluor 488 goat anti-rat and Alexa Fluor 546 goat anti-mouse [Molecular probes ...
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bioRxiv - Neuroscience 2021Quote: ... plus 5% normal goat serum for 1 hr at RT before 3 d incubation in primary antibody diluted in block (1:1,000, chicken anti-GFP, Abcam, ab13970; 1:200 rabbit anti-cleaved caspase 3, EMD Millipore, AB3623). Sections were washed several times in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were then incubated in 5% normal donkey serum (NDS) for 1 hour before primary antibody incubation (mouse anti-GFP, 1:1500, Life Technologies; rabbit anti-TH, 1:2000, Millipore) overnight at room temperature with NDS and PBS-Tx ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were incubated at room temperature for 1 h with Cy2/Cy3-conjugated anti-mouse or anti-rabbit secondary antibody (1:50; Millipore AP182C). After three washes in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... cells were blocked-permeabilized in 5% gelatin from cold water fish skin and 0.1% triton X-100 for 1 h followed by incubation with primary antibodies (mouse anti-acetylated tubulin, and rabbit anti-Neurofilament heavy chain, Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were incubated with primary antibodies overnight at 4°C (rabbit anti-ORF2 mAbs at 1:1000; mouse anti-Flag M2 (Sigma F1804) at 1:2000) ...
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bioRxiv - Plant Biology 2020Quote: ... The protein was separated on a 10% SDS-PAGE gel and further analyzed by immunoblotting using anti-His and anti-GST antibody (Sigma-Aldrich).
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bioRxiv - Pathology 2020Quote: ... Membranes were then incubated with the corresponding secondary antibodies (rabbit anti-mouse IgG/HRP, Dako, #P0260 and goat anti-rat IgG/HRP, Sigma, #A9037) at a 1:5000 dilution ...
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bioRxiv - Pathology 2020Quote: ... The blocking solution used for the anti-phospho-Smad2 and anti-phospho-Smad3 antibodies contained 5% bovine serum albumin (Sigma-Aldrich). The primary antibodies applied to HK-2 cells were anti-α-smooth muscle actin (α-SMA ...
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bioRxiv - Microbiology 2020Quote: ... the plates were washed and incubated with an anti-IpaA (dilution 1/2000e) polyclonal primary antibody3 or anti-vinculin (dilution 1/2000e) Vin11Vin.5 monoclonal antibody (Sigma-Aldrich) in binding buffer containing 0.2% BSA for one hour at room temperature ...
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bioRxiv - Genetics 2019Quote: ... and incubated overnight at 4 °C with primary anti-myosin heavy chains antibody (Monoclonal Anti-Myosin skeletal, Fast, Clone My-32, Mouse Ascities Fluid, M4276, Sigma-Aldrich) diluted (1:400 ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Primary antibodies (rabbit anti-phospho-ERK, Cell Signaling Technology #9101, 1:1000; mouse anti-α-tubulin, Sigma-Aldrich #T9026, 1:5000) were diluted in Odyssey Blocking Buffer containing 0.2% Tween 20 ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1h and probed with the following primary antibodies: guinea-pig anti-Oddskipped (1/100, in house) and mouse anti-actin-HRP (1/20 000, Sigma A3854). After incubation with HRP-conjugated anti-guinea-pig secondary antibody (1/10 000 ...
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bioRxiv - Neuroscience 2022Quote: ... before the addition of 17.6 μl anti-KIF1A or anti-PLCB1 antibody and 100 U of Benzonase nuclease (Novagen 70746-3). The mixture was incubated overnight at 4°C on a rotating wheel ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then washed with PBS-T and incubated with anti-rabbit or anti-mouse peroxidase-labeled antibodies (1:10000, Sigma-Aldrich). After extensive washing with PBS-T ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were blocked in 5% horse serum for one hour and incubated with anti-MYC primary antibody (SCBT, 9E10) at a 1:500 dilution or anti-FLAG M2 (Sigma, F1804) at a 1:400 dilution overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Eventually, the blots were probed with indicated primary antibodies: anti-HA (261883, Thermo Fischer) and anti-β-actin (A2228, Sigma-Aldrich) antibodies overnight at 4°C followed by incubation with secondary anti-rabbit (711-035-152 ...
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bioRxiv - Cell Biology 2024Quote: Samples were incubated with secondary antibodies conjugated with PLA probes MINUS and PLUS: the PLA Probe anti-Mouse PLUS and anti-Rabbit Minus (Sigma-Aldrich). The incubation with all antibodies was carried out in a humidified chamber for 1 h at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... After incubation with horse-radish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit-HRP and goat anti-mouse-HRP, Sigma-Aldrich) at room temperature for 1 h ...