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Citations for Millipore Sigma :
4751 - 4800 of 10000+ citations for IL 3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 50ng of esiRNA Human KCNH2 (Cat. EHU080951, Milipore Sigma) or siRNA Universal Negative Control per 5.0 x 104 hiPSC-CMs using Mission® siRNA Transfection Reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with 270ng of esiRNA Human GJA1 (Cat. EHU105621, Millipore Sigma) or siRNA Universal Negative Control (Cat ...
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bioRxiv - Immunology 2023Quote: ... OVOL1 protein expression were determined by rabbit anti-human OVOL1 antibody (Sigma Aldrich).
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bioRxiv - Pathology 2023Quote: ... the anti-human CD31 primary antibody (clone JC70, Cell Marque, Millipore Sigma, USA), and the detection with impact DAB (Vector Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... and 1% (v/v) human serum from platelet poor plasma (P2918; Sigma-Aldrich) and seeded into a T75 cell culture flask that was previously coated with 0.1% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... 2 μm latex green beads were incubated with human IgM (I-8260, Sigma), then in rabbit anti-human IgM (Sigma 270A ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.15×106 of murine stromal cells expressing human delta like ligand 1 (Sigma) were combined with 7.5×103 of sorted and enriched KO-/- ...
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bioRxiv - Biochemistry 2023Quote: ... Zymosan A was opsonized by incubation with human serum (Sigma-Aldrich, no. H4522) at 37 °C for 30 min ...
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bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
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bioRxiv - Cell Biology 2023Quote: ... human iPSCs were cultured on gelatin-coated dishes in differentiation medium (GMEM (Sigma) supplemented with 1mM sodium pyruvate ...
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bioRxiv - Cell Biology 2023Quote: ... MDM were cultured in RPMI 1640 medium supplemented with 10% human serum (Sigma). VSVDG pseudotyped HIVD1 GFP virus was produced by transfecting of 293T with GFPDencoding genome CSGW ...
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bioRxiv - Pathology 2023Quote: Human hepatoma cell lines were grown in Minimum Essential Medium (MEM, Sigma Aldrich) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2023Quote: ... The shRNAs targeting human MARCHF8 were ordered from Sigma-Aldrich (St. Louis, MO). The sgRNAs targeting Marchf8 were designed using the web-based software ChopChop (chopchop.cbu.uib.no ...
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bioRxiv - Cell Biology 2023Quote: ... glass coverslips were coated with 10 µg/mL human plasma fibronectin (FC010, Millipore) overnight at 37◦C ...
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bioRxiv - Bioengineering 2023Quote: Insulin-azide and insulin-alkyne were prepared from recombinant human insulin (EMD Millipore) by selective acylation of the B29Lys residue as previously reported.33 ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected with 270ng of esiRNA Human KCNJ2 (Cat. EHU019661, Millipore Sigma) or non-targeting control (NTC ...
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bioRxiv - Immunology 2024Quote: ... MILLIPLEX Map Human cytokine/chemokine Magnetic Bead Panel – Premixed 41 Plex (Millipore Sigma), run on a MAGPIX® xMAP instrument according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Human spinal cord samples were homogenized with a dounce homogenizer (Sigma Aldrich, D8938) in 1mL nuclei lysis buffer using the Nuclei Pure Prep Nuclei Isolation Kit (Sigma Adlrich ...
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bioRxiv - Cell Biology 2024Quote: ... primary human skeletal muscle cells were seeded and differentiated on laminin (Sigma Aldrich) coated 4-well Nunc Lab-Tek II Chamber Slides (Thermo Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... We obtained the antibodies through commercial suppliers: IgA from human serum (Sigma-Aldrich), IgM from human serum (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: Fresh or cryopreserved primary adult human hepatocytes (BeCytes Biotechnologies, BiolVT or Sigma-Aldrich) were resuspended in hepatocyte spheroid medium (HSM) ...
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bioRxiv - Immunology 2024Quote: ... coated with human placental type IV collagen (Sigma-Aldrich, St. Louis, MO, USA). The cells were cultured using Ex Plus in both the basolateral and apical compartments for 1 week ...
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bioRxiv - Microbiology 2024Quote: ... Coverslips with C3bi-coated beads were incubated with rabbit anti-human IgM (Sigma) at 1:250 ...
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bioRxiv - Microbiology 2024Quote: Purified human PMNs were incubated with phorbol 12-myristate 13-acetate (PMA) (Sigma) 100nM for 4h ...
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bioRxiv - Immunology 2020Quote: ... were cultured at a concentration of 1 million cells per ml in complete media on pre-incubated anti-CD3/anti-CD28 bound plates in IL-2 (20ng/ml) (Sigma-Aldrich) for 2 days and then just IL-2 (20ng/ml ...
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bioRxiv - Physiology 2019Quote: The interactions between TRPA1 and IL-R1 in nodose ganglia cultures were detected by PLA with Duolink in situ kit (Sigma- Aldrich). Primary nodose ganglion cells were harvested and cultured on glass coverslips as described above ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 x 105 IL-7 dependent primary Irf4−/− preB-I cell cultures were resuspended in 400 µL RPMI medium (D5030, Sigma-Aldrich) with 600 µL viral supernatant and 1.5 µL polybrene and spun in culture plates at 2700 RPM for 90 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Immunology 2022Quote: ... PBMCs cultures not supplemented with IL-2 overnight were stimulated with 25 ng/ml phorbol 12-myristate-13-acetate (PMA) (Sigma-Aldrich) and 1 μg/ml ionomycin (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: ... LP cells were incubated for 5 h with 20 ng/ml PMA and 1 μg/ml ionomycin in the presence of 10 μg/ml Berfeldin A (Sigma, IL). For FACS analysis ...
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bioRxiv - Cell Biology 2019Quote: Genomic DNA was isolated from the si-NC and si-Dnmt3aos-transfected M(IL-4) macrophage cells and was then treated with bisulfite using the Imprint DNA Modification Kit (Sigma-Aldrich). Bisulfite sequencing and pyrosequencing were conducted by Shanghai Sangon Biotech Corporation ...
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bioRxiv - Microbiology 2021Quote: ... All SEC-FPLC described in this study was performed on a GE Healthcare AKTA PV-908 FPLC system (Chicago, IL) on Superdex S200 10/300 GL (Sigma-Aldrich) and Superose 6 10/300 GL (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently transduced twice with viral supernatant within 48 hrs in the presence of 50 IU/mL IL-2 and 6 mg/mL polybrene (Sigma-Aldrich). TCR-transduced T cells were expanded by stimulation with anti-CD3/CD28 Dynabeads (500,000 beads/106 cells ...
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bioRxiv - Immunology 2021Quote: ... SRP4895A), N-(p-Amylcinnamoyl)anthranilic acid (ACA, A8486), PP2 (P0042), interleukin-8 (IL-8, CXCL8 SRP3098) were purchased from Sigma-Aldrich (Sigma-Aldrich Company Ltd. ...
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bioRxiv - Immunology 2020Quote: ... the wells were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) added to the wells ...
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bioRxiv - Immunology 2022Quote: ... All cocultures were maintained in SCGM supplemented with IL-2 (120 IU/ml) and in the presence of 10 µM raltegravir (Sigma-Aldrich) and 0.4 µM nevirapine (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... virus supernatant was aspirated and preactivated PBMCs (0.5-0.8 x 106/well) in TexMACS medium supplemented with 100 IU/ml IL-2 and 6 ng/ml polybrene (Sigma-Aldrich) were added to each well ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were activated with interleukin-2 (IL-2) 450 UI/mL (Proleukin, Novartis pharma) and polybrene 2 µg/mL (Sigma-Aldrich) during at least 2 h before infection.
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bioRxiv - Neuroscience 2024Quote: The viral particle stock was diluted in sterile saline (0.9% NaCl) (Hospira, Lake Forest, IL) with 0.01% Fast Green (Sigma, St. Louis, MI). A 10-µl Hamilton syringe (Hamilton Company ...
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bioRxiv - Neuroscience 2024Quote: ... we used our LPS-sensitized model of HI described above and delivered IP 1.5 IU/g of hCG diluted in normal saline (Sigma-Aldrich, St. Louis, MO, Catalog no. CG-10) or normal saline alone as vehicle control two hours prior to LPS administration (Figure 2A) ...
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bioRxiv - Plant Biology 2021Quote: ... blocked with 3% milk for 1 hour at room temperature and probed with anti-FLAG M2 monoclonal antibody (mouse, Sigma F1804, 1:2000 in 3% milk/PBST), or blocked with 5% BSA for 1 hour at room temperature and probed with anti-HA antibody (rabbit ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22, CRP; Millipore Sigma - RAB0096-1KT). In brief ...
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bioRxiv - Immunology 2021Quote: Degranulation and cytokine production of NK cell subsets were determined after cytokine stimulation o/n with IL-12 (10ng/ml, Sigma Aldrich, USA) and IL-18 (5ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... and carbonic anhydrase (29 kD) from a combination of two kits (GE Healthcare, catalogue No. 28-4038-41, Chicago, Illinois, IL, USA and MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Neuroscience 2021Quote: ... and phosphorylated-tau 181 (p-Tau) were measured according to manufacturer specifications (Luminex xMAP technology; EMD Millipore, Chicago, IL, USA: HNABTMAG-68K), modified by a 1:10 dilution of CSF ...
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bioRxiv - Microbiology 2021Quote: ... The resulting hybridoma cells were plated onto 96-well plates and cultured in HAT selection medium (Hybridoma-SFM [Life Technologies, Grand Island, CA, USA]; 10% FBS; 1 ng/mL mouse IL-6; 100 μM hypoxanthine [Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2019Quote: Synaptoneurosome samples and a protein ladder were separated on 4-20% SDS-PAGE gels (Pierce, Rockford, IL) and transferred to polyvinyldenine fluoride (PVDF) membranes (Millipore, Billerica, MA). The blots were blocked in PBS containing 0.05% Triton-x (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... conjugated anti-histidine was added and color developed by addition of and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St. Louis, MO, USA). Plates were read at an OD of 450 nm with a SpectraMax Plus plate reader (Molecular Devices ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasma was stored at −30°C for analysis of leptin and insulin (CrystalChem Inc., Chicago, IL, USA) and triglyceride content (Roche triglyceride reagent, Sigma glycerol standard).