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Citations for Millipore Sigma :
4701 - 4750 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 2-Amino-2-norbornanecarboxylic acid (BCH; Sigma, #A7902), α-(Methylamino)isobutyric acid (MeAIB ...
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bioRxiv - Immunology 2022Quote: ... 100 mM 2-deoxyglucose (2-DG) (Sigma-Aldrich). Non-glycolytic acidification of media (ECAR following injection of 2-DG ...
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bioRxiv - Immunology 2024Quote: ... 50 μM 2-ME (Sigma 60-24-2). Cell Stimulation Cocktail (Tonbo # TNB-4975-UL100 ...
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bioRxiv - Bioengineering 2024Quote: 2-(Dodecylthiocarbonothioylthio)-2-methylpropionic acid (DDMAP, Sigma-Aldrich), ethylene glycol (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... and 2% phosphatase inhibitor mixture 2 (Sigma; P5726). 10-15 μg of protein was separated on 12% Bis-Tris-PAGE (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... 2 mL of 2 mg/mL Papain (Sigma) was added to 30 mg of tissue and incubated at 37 °C for 20 minutes ...
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bioRxiv - Microbiology 2024Quote: ... A freshly prepared 2-mercaptoethylamine (2-MEA; Sigma) solution (750 mM ...
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bioRxiv - Neuroscience 2023Quote: ... Brains of P0 pups were kept in 4% PFA in 4°C overnight and then transferred to 4°C 30% sucrose (Sigma-Aldrich, S5016) with 0.01% sodium azide (Fisher ...
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bioRxiv - Cell Biology 2023Quote: Whole fetal livers (FL) were fixed during 24h at 4°C in 4% formaldehyde and then embedded in 4% low-melting agarose (Sigma #A9539-500G). One hundred µm sections were made using a vibratome (Leica #VT1200 ...
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bioRxiv - Bioengineering 2019Quote: ... cells were permeabilized via 0.02% Tween 20 for 20 minutes and incubated overnight at 4°C with rabbit anti-SLCO1B3 (OATP1B3) primary antibody (2-μg/mL working concentration, HPA004943, Sigma-Aldrich Canada, Oakville, Ontario, Canada). Goat anti-rabbit AlexaFluor 647-conjugated secondary antibody was then applied for two hours at room temperature (1:500 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with 15 μM substrate 2′ (4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-NANA) in 37°C warmed sterile filtered TBS (Sigma Aldrich; St. Louis, MO). Fluorescence intensity (excitation ...
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bioRxiv - Biophysics 2023Quote: ... and ultrapure H2O and finally incubated in a solution of poly-T ssDNA (100 nM) in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (10 mM HEPES, NaCl 50 mM, pH 7, Sigma-Aldrich, St Louis, MO, USA) for 1 h at approx ...
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bioRxiv - Microbiology 2024Quote: ... Cells were cells fixed with 4% paraformaldehyde (ProSciTech, Cat. no. C004) in PBS for 30 min at RT and blocked with 2% BSA (Sigma-Aldrich, Cat. no. A8022-10G) in PBS (BSA/PBA ...
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bioRxiv - Neuroscience 2023Quote: ... Subsequent cytokine measurements interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were done using Milliplex magnetic bead panel (Millipore Sigma, MA, US) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The loading reaction (480 µL) was spun in a pre-rinsed Amicon Ultra 0.5 mL Centrifugal Filter with 100 kDa MWCO (Merck Millipore, 6 000 g, 6 min). After two washes with water (480 µL ...
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bioRxiv - Cell Biology 2019Quote: ... (TDP43: 5’-gcaaagccaagaugagccu-3’ and EGFP control: 5’-gcaccaucuucuucaagga-3’; Sigma). Silenced cells were collected by trypsinization ...
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bioRxiv - Cell Biology 2022Quote: ... 5% glycerol) supplemented with 5 μL Benzonase nuclease (Sigma, E1014-25KU), 1 mM phenylmethanesulfonyl fluoride ...
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bioRxiv - Cell Biology 2022Quote: ... livers were perfused with 5 μg/200 ml ADAMTS-5 (Sigma) for 1h ...
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bioRxiv - Plant Biology 2019Quote: ... a 5 mm agarose (5 g/L, Sigma Aldrich Chemie, CHE) layer was poured into a Petridish (9 cm diameter ...
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bioRxiv - Cell Biology 2020Quote: ... and resuspended in 100 μl of 5% 5-Sulfosalicylic acid (Sigma) solution ...
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bioRxiv - Immunology 2021Quote: ... for 5 mins and 5 µM fMLP (F3506, Sigma-Aldrich, USA) for 30 mins at 37°C and then stained with antibodies as mentioned above ...
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bioRxiv - Immunology 2019Quote: ... histone methyltransferases by 5′-deoxy-5′-methylthioadenosine (MTA; 1mM; Sigma-Aldrich) and histone demethylasis by pargyline (3 µM ...
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bioRxiv - Immunology 2024Quote: ... and resuspended in 100 μl of 5% 5-Sulfosalicylic acid (Sigma) solution ...
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bioRxiv - Cell Biology 2023Quote: Fresh cells were lysed with 5% 5-sulfosalicylic acid (Sigma-Aldrich) solution ...
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bioRxiv - Microbiology 2024Quote: ... and blocked in 5% milk or 5% BSA (Sigma-Aldrich, #A9647) in PBS-T for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and 5×10−5 M β-mercaptoethanol (Millipore-Sigma, Oakville ON). 38B9 cells were stimulated in culture with 10 μg/ml lipopolysaccharide from E ...
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bioRxiv - Immunology 2024Quote: ... and 5×10−5 M β-mercaptoethanol (Millipore-Sigma, Oakville ON). 38B9 cells were stimulated in culture with 10 μg/ml lipopolysaccharide from E ...
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bioRxiv - Developmental Biology 2019Quote: Cells undergoing DNA replication in S phase were marked by incorporation of 5-ethynyl-2’-deoxyuridine (EDU) (Sigma, St. Louis, MO. Cat # 900584-500MG). A stock concentration of 32.5 mM EDU was made by adding EDU to sterile saline solution with the addition 1:1000 5 M NaOH and heating to 42°C for 30 to 60 minutes to dissolve ...
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bioRxiv - Biophysics 2020Quote: ... All chemicals for matrix preparation such as methanol, LC-MS grade water, and DHB (2, 5-dihydroxycinnamic acid) were procured from Sigma-Aldrich (St. Louis, MO) while TFA (trifluoroacetic acid ...
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bioRxiv - Plant Biology 2021Quote: ... 5-day-old Col-0 and lnccobra1-2 seedlings were crosslinked in PBS with 1% formaldehyde (v/v) (Sigma-Aldrich; St. Louis, MO, USA) added and placed under vacuum for 10 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... Aortas were then washed in 78% methanol for 5 minutes under gentle movement for a total for 3 times before incubating in a 2% ORO solution (Sigma-Aldrich, St Louis, MO) for 60 minutes ...
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bioRxiv - Immunology 2022Quote: BM cells from mice were isolated by flushing femurs and tibias with 5 ml PBS supplemented with 2% heat-inactivated fetal bovine serum (FBS) (Sigma Aldrich, Lenexa, KS, USA). The BM cells were centrifuged once and then re-suspended in tris-ammonium chloride at 37°C for 30 s to lyse red blood cell ...
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bioRxiv - Immunology 2022Quote: ... for 2 h at room temperature and then blocked for 1 h at room temperature with 5 % neonatal calf serum (Millipore Sigma, St. Louis, MO) in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... These animals were raised at 20°C throughout the experiment and transferred to nematode growth medium (NGM) plate supplemented with 250uM 5-fluoro-2′-deoxyuridine (FUDR; Sigma-Aldrich, St. Louis, USA) to prevent progeny production ...
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bioRxiv - Plant Biology 2020Quote: ... frozen leaf samples were ground in liquid nitrogen and incubated in 4 ml lysis buffer (50 mM Tris-HCl pH7.5, 150 mM NaCl, 5% glycerol, 0.05% NP-40, 1 mM MgCl2, 0.5 mM PMSF, 1X Sigma protease inhibitor, 10 mM DTT, 2% PVPP) at 4 °C for 15 min using a rotator to form a homogeneous suspension ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-BUBR1-p620 (custom raised by Moravian, 1:1000) and mouse anti-α-Tubulin (clone B-5-1-2, T5168, Sigma-Aldrich, 1:10000). Secondary antibodies used were goat anti-mouse IgG HRP conjugate (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: Animals were anaesthetised and 2-3 mL of fresh collagenase XI solution was injected in the clamped end of the bile duct inserted in the duodenum (Collagenase from Clostridium histolyticum, Type XI, 2-5 FALGPA units/mg solid, >1200 CDU/mg solid; C7657 Sigma, St. Louis, MI, USA) at 1000 U/mL in Hank’s Balanced Salt Solution (HBSS without MgCl2 and CaCl2 ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µg equivalent of protein from each sample were then treated with 5 mM tris (2-carboxyethyl)phosphine hydrochloride (Sigma-Aldrich Cat No: C4706) to reduce disulfide bonds and the resulting free cysteine thiols were alkylated with 10 mM chloroacetamide (Sigma Aldrich Cat No ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... determined as the amount of 5-bromo-2′-deoxy-uridine (BrdU) incorporation (Roche BrDU Labeling and Detection Kit II, Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM recombinant calmodulin (Enzo, cat. BML-SE325-0001) and 2 mM Adenosine 5’-triphosphate (ATP) disodium salt hydrate (Sigma-Aldrich, cat. A1852-1VL) for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and 3 μg/mL + 7 μg/mL FRD+U (5-Fluoro-2′-deoxyuridine, Sigma-Aldrich, Cat# F0503 and Uridine, Sigma-Aldrich, Cat# U3003-5G). 120 µL of cell suspension was carefully plated onto each coverslip and allowed to adhere for 2 hours at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 2 mg equivalent of protein from each sample was then reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich Cat No: C4706) for 30 minutes at room temperature and alkylated with 10 mM chloroacetamide (CAA ...
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bioRxiv - Cancer Biology 2023Quote: ... des-Arg9-Leu8]-BK (B1R antagonist) and 3-(5′-Hydroxymethyl-2′-furyl)-1-benzyl indazole were purchased from Sigma-Aldrich (St. Louis, MO, USA). 8-Bromoguanosine 3′ ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with primary antibody to stain tubulin (mouse anti-α-tubulin B-5-1-2 mAb, Sigma-Aldrich, T5168, dilution 1:250) for 4 h at room temperature ...
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bioRxiv - Genomics 2022Quote: ... with the modifications that 350 µl Buffer RLT Plus + β-mercaptoethanol was added to each Lysing D matrix tube (5 µl of β-mercaptoethanol (2-Mercaptoethanol, Sigma-Aldrich, M6250), added to 500µl of Buffer RLT Plus (from kit ...
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bioRxiv - Cancer Biology 2023Quote: ... exponentially growing cells were cultured in media containing 30 μM 5-iodo-2′-deoxyuridine (IdU) at 37°C for 4h (Sigma-Aldrich, St. Louis, MO). After 4h ...
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bioRxiv - Microbiology 2023Quote: ... a mouse infected with the modified parasite clones was given drinking water containing 2 mg/ml of 5-fluorocytosine (Sigma-Aldrich, cat. no. F6627) in a dark bottle ...
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bioRxiv - Neuroscience 2023Quote: ... Below is a list of the drugs used in this experiment and delivered via a gravity fed perfusion system at 2–5 ml/min: Dopamine hydrochloride (Sigma-Aldrich; Saint Louis, MO), forskolin (Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with 0.013% DMSO with or without 100 nM pan-PLD inhibitor 5-fluoro-2-indolyl des-chlorohalopemide (PLD1/2i, Sigma Aldrich, St. Louis, MO, USA) or 500 nM PLD1-specific inhibitor VU0155069 (PLD1i ...
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bioRxiv - Cell Biology 2019Quote: ... 6 ul of 10% ammonium persulfate (APS; A3678, Sigma-Aldrich) and 4 ul of fN ...