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Citations for Millipore Sigma :
4601 - 4650 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 1% input and 100% of IP were used for SDS-PAGE and probed with anti-FLAG antibody (1:3000, Sigma F7425).
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bioRxiv - Microbiology 2021Quote: ... Spore suspensions were then incubated for 1□h with 1:20 donkey anti-mouse IgG antibody coupled to 12-nm gold particles (Sigma G7777) in PBS–1% BSA for 1□hr at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunohistochemistry was performed with antibodies diluted in 1% bovine serum albumin in tris-buffered saline directed to Pth1r (1:50 dilution, Sigma SAB4502493) or with a nonspecific IgG (control) ...
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bioRxiv - Neuroscience 2021Quote: ... The corresponding secondary antibodies (coupled to Alexa Fluor® 488 or 568, LifeTechnologies, 1:800) and Hoechst (Sigma-Aldrich, 1/800) for 2 hours at RT ...
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bioRxiv - Cell Biology 2022Quote: ... cleared by centrifugation and incubated overnight with anti-acetylated lysine antibody (1:100) or for 1 hour with 50μL of pre-washed α- FLAG M2 affinity gel agarose slurry (Sigma, A2220) on a rotator at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1 % BSA ...
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bioRxiv - Biophysics 2022Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1 % BSA ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then incubated with primary antibodies overnight at 4°C: anti-Alpha Tubulin (1:200, DSHB 12G10) and β-catenin (1:10,000 dilution, Sigma ABE208). The membrane was washed three times using PBST ...
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bioRxiv - Physiology 2022Quote: ... and incubated with antibodies against GluN1, GluN2A, GluN2B, and PARP1 (1:1000, Cell Signalling, USA) or calnexin (1:5000, Sigma-Aldrich), followed by HRP-conjugated secondary antibody (Cell Signalling ...
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bioRxiv - Molecular Biology 2024Quote: Antibodies used for immunoblotting in this study include rabbit polyclonal anti-GFP (ab290, Abcam, 1:2,500 or Sigma, G1544, 1:1000), mouse monoclonal anti-GFP (JL8 ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 h and incubated overnight at 4°C with the specific primary antibody: mouse anti-NeuN 1:200 (Millipore, MAB377), rabbit anti-GFAP 1:1000 (Dako ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.25% Triton X-100 for 30 min at RT and subsequently incubated in 0.1% Triton X-100 and 1% NS in PB with the following primary antibodies: mouse monoclonal anti-NeuN (1:1000; Millipore, #MAB377), anti-SMI32 (specific for non-phosphorylated neurofilament H ...
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bioRxiv - Genetics 2024Quote: ... Membranes were incubated overnight at 4°C in primary antibodies (Flag, F7425, Sigma-Aldrich, 1:1000; HDAC2, ab32117 Abcam, 1:1000; acetylated Tubulin, T6793 Sigma, 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Tubulin labeling was carried out with a mouse monoclonal anti-acetylated tubulin antibody (T6793-6-11B-1, Sigma-Aldrich, 1:250) and followed by either a goat anti-mouse IgG Cy3 (115-165-166 ...
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bioRxiv - Genetics 2023Quote: ... Primary antibody was anti-acetylated α-tubulin mouse monoclonal clone 6-11B-1 (1:500 dilution, Sigma, T7451-200, lot #077M4751V). Secondary antibody was Alexa Fluor 488 donkey anti-mouse IgG (1:1,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were fixed with ice-cold acetone/methanol (1:1 v/v) and a specific antibody binding was blocked with 5% BSA (w/v, Sigma-Aldrich). Organoids were then incubated with primary antibodies against acetylated α-tubulin (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... then with primary antibodies (anti-LC3 A/B-12741, Cell Signaling, 1:1500 and anti-alpha tubulin-T6199, Sigma, 1:10000), followed by fluorescent secondary antibody incubation ...
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bioRxiv - Biophysics 2023Quote: ... blocked in Odyssey Blocking Buffer (Licor #927-50000) at room temperature for 1 hour and incubated with primary antibodies against total Cx43 (1:5000, C6219 rabbit, Sigma Aldrich) and GAPDH (1:5000 ...
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bioRxiv - Cell Biology 2023Quote: ... An additional washing step was followed by a 1 h incubation at RT with the primary mouse anti-c-myc antibody (500 µl, 1:200, Sigma Aldrich). Further ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by a 1 hour incubation with an anti-Lkb1 (1:5000, CST, 13031) or anti-NQO1 primary antibody (Nqo1 (1:100, HPA007308, Sigma-Aldrich) in Leica diluent (Leica ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216, Anti-FLAG; 1:2000 dilution, Sigma-Aldrich #F1804 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were treated 1 h prior to infection with a 1:50 dilution of monoclonal antibody 1987 clone P4C10 (EMD Millipore) directed against β1 integrins.
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were diluted in blocking reagent to detect Pax7 (Developmental Studies Hybridoma Bank, 1:10) and laminin (Sigma, 1:50). After 16h incubation at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed with TTBS and incubated for 1 h at room temperature with isotype specific secondary antibodies anti-mouse IgG (1:10,000; Sigma-Aldrich, A4416) and anti-rabbit IgG (1:80,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained for 1 hr at room temperature using primary antibody for anti-CDHR5 (1:200; Sigma catalog no. HPA009081), anti-CDHR2 (1:75 ...
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bioRxiv - Cell Biology 2022Quote: ... and then incubated with primary antibodies in blocking buffer [1:400 homemade rabbit anti-RBM20,15 or 1:500 anti-α-actinin (cat# A7811; Sigma-Aldrich)] overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... which were blocked in 5% dry milk powder in TBS-T for 1 hour and then incubated in primary antibody solutions (Rabbit anti NUDT6, Proteintech 1:800; mouse anti FGF2, Millipore, 1:500; mouse anti ß-actin, Sigma, 1:7500 ...
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bioRxiv - Physiology 2023Quote: ... Collected adrenal sections were probed with antibodies for TLQP-21 (rabbit anti-TLQP-21, 1:100, 20) or Tyrosine Hydroxylase (sheep polyclonal TH, 1:100, Millipore Sigma) overnight at 4°C in a humidified chamber ...
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bioRxiv - Neuroscience 2023Quote: ... and 5% normal goat serum in MAB for 30 min at RT and incubated with alkaline phosphatase-conjugated anti-DIG antibody (1:10000 for oxt, 1:1000 for other genes, Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt, Germany; SOX9, 1:1000, #AB5535 EMD Millipore, Burlington ...
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bioRxiv - Physiology 2023Quote: ... the secondary antibody was applied by incubating the slides for 1 hour at room temperature with blocking buffer containing Alexa Fluor 647-conjugated goat anti-mouse antibody (1:1000, Sigma, SAB4600354). The slides were then briefly rinsed in PBS-Tw before a 10-minute incubation in PBS-Tw containing 1:2000 SYTOX Orange (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with anti-Sulf-1/2 primary antibody (anti-Sulf-1: rabbit polyclonal, Sigma, SAB1410410, anti-Sulf-2: rabbit monoclonal clone 2B4, Sigma, MABC584) overnight at 4 °C ...
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bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2% Triton X-100 diluted in PBS for 1 h before incubation with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-LCN-2 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... The final dilution of the primary antibody was 1:2,000 for anti-HA tag (Covance) and 1:3000 for anti-β-actin (Millipore-Sigma).and K-Rta (65).
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bioRxiv - Neuroscience 2024Quote: ... the membrane was blocked with 5% BSA and incubated with primary antibodies against Tuj-1 (dilution 1:10,000, Sigma, Catalog # T8578) and pan-Ago (dilution 1:1200 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained for microtubules by 1 hr incubation with a primary antibody against α-tubulin (Sigma-Aldrich, T5168, 1:200). Following three washes ...
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bioRxiv - Cell Biology 2024Quote: ... dilution 1:10) cell-surface antibodies after a non-specific sites’ saturation step with Gamma Immune (Sigma Aldrich, dilution 1:2). Isotype controls (Miltenyi Biotech) ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated for 1 hour at room temperature in the following secondary antibodies diluted in 1% Normal Goat Serum with DAPI (1:10000, Sigma, D9542): Alexa Fluor 488 goat anti-mouse (1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... 2 hours), α-SodA (1:5000, 30 minutes), α-VSV-G (1:3000, 1.5 hours) and a goat α-rabbit secondary antibody (Sigma, 1:5000, 45 minutes). Clarity Max Western ECL substrate (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... Overnight incubation with primary antibodies diluted in blocking/permeabilisation buffer (GFP – 1:1000 Ab13970 Abcam, TH – 1:1000 Ab152 Sigma, IL33 – 1:50 AF3626 R&D) were performed overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... followed by a 2 h incubation at 37 °C with the respective primary antibody (anti-G3BP2, 1:600 [Proteintech®, 16276-1-AP]; anti-GAPDH-AF488 [Sigma-Aldrich, MAB374-AF488], 1:500). Cells were washed and incubated with secondary antibody (mouse anti-rabbit IgG-CFL 647 ...
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bioRxiv - Microbiology 2022Quote: ... or Muller-Hinton Broth (MHB II, Sigma-Aldrich, St. Louis, Missouri, USA) with linear shaking in an Elx808 Absorbance Reader (Biotek Instruments ...
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bioRxiv - Bioengineering 2019Quote: ... 5 mM of manganese (II) chloride (MnCl2) (Sigma-Aldrich, cat no. 244589), 0.1 mM of zinc chloride (ZnCl2 ...
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bioRxiv - Neuroscience 2019Quote: Black Gold staining (Black Gold II myelin staining kit, Millipore, Burlington, MA) was performed according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Tamoxifen and Collagenase II were purchased from Sigma (St Louis, MO, USA). Dulbecco’ s modified Eagle’ s medium (DMEM) ...
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bioRxiv - Biochemistry 2021Quote: ... anti-RNA Pol II (clone CTD4H8, EMD Millipore, cat. no. 05-623), anti-RNA Pol II CTD phospho S5 (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... and RNA Pol II (clone CTD4H8, EMD Millipore, cat. no. 05-623).
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bioRxiv - Cell Biology 2021Quote: ... primary tumour from was disaggregated in 1.5 mg/mL collagenase II (Sigma) and 20 μg/mL hyaluronidase (Sigma ...