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Citations for Millipore Sigma :
4551 - 4600 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibodies goat anti-Olig2 (1:100, R&D AF2418) and rabbit anti-NeuN (1:100, Millipore ABN78) for two days at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... We use a combination of antibodies (rabbit anti-parvalbumin, 1:5000, PV235, Swant + biotin-conjugated lectin from WFA, 1:250, L-1516, Sigma-Aldrich). On the day after ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated overnight at 4 °C in vehicle with primary antibodies (mouse anti-Syt7, 1:200, Neuromab N275/14; rabbit anti-TH, 1:500, Millipore AB152). After 3 washes for 30 minutes in vehicle ...
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bioRxiv - Neuroscience 2021Quote: ... washed as above described and re-blotted using mouse anti-β-actin antibody (Sigma-Aldrich, USA; 1:2500, 1 h, RT). Again ...
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bioRxiv - Neuroscience 2019Quote: ... blocked using 1% BSA in TBST and incubated with primary anti-TH antibody (dilution 1:500; AB152, Merck Millipore, Schaffhausen, Switzerland). 24h later ...
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bioRxiv - Developmental Biology 2019Quote: ... Western blots were incubated with the following primary antibodies for 1 hour at room temperature in 3% milk: 1:4000 M2 Flag (Sigma 3165),1:4000 H3 (Cell Signaling 4499) ...
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bioRxiv - Neuroscience 2019Quote: ... Nitrocellulose membranes were washed 3 times for 10 min with TBST and incubated with HRP-conjugated secondary antibodies for 1 h at RT (1:1000; Sigma-Aldrich, St ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 rabbit anti-β-tubulin antibody (Sigma Aldrich, T5293) in PBS + 1% BSA ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1% BSA ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were rinsed 6 times in 1x PBS before being incubated with fluorescence-labeled secondary antibody (diluted in blocking buffer at 1:500 dilution ratio with 1/10000 Hoechst 33258, Sigma #94403) for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by incubation with the primary antibodies diluted in a 1:1 solution of blocking buffer to PBS with 0.2% Tween (PBST) (Sigma-Aldrich, P7949) overnight at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour before incubation with primary antibodies: rabbit anti-NogoA (1:200; Supplementary Table 4) and mouse anti-GFAP (1:500; Sigma-Aldrich), for 72 hours at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... TBST) for 1 hr at room temperature and then stained with primary antibodies against α-tubulin (1:1000; Sigma Aldrich, T6199) and GAPDH (1:5000 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with a 1:2000 dilution of anti-phospho-Histone H2A.X (Ser139) clone JBW301 antibody (Millipore, 05-636-1) for an hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... nonfat dry milk in PBS-T for 1 h Membranes were incubated with mouse anti-FLAG M2 antibody (1:500, Sigma-Aldrich) or mouse anti-α-tubulin B512 antibody (1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... The samples were incubated overnight at 4 °C with the indicated primary antibody prepared in blocking/permeabilization solution (with 0.1% Triton X-100): rabbit polyclonal antibody against GLUT-1 (1:250; Millipore, 07-1401), rabbit monoclonal antibody against HK-1 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1 h with A594-conjugated secondary donkey anti-goat antibody (1:500, EMD Millipore). Sections were then washed in PBS and blocked with 10% normal goat serum (Dako ...
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bioRxiv - Biochemistry 2021Quote: ... mouse APC-conjugated antibody directed against dsRNA J2 (IF 1:200, Scicons, 10010500) mouse anti-3xFlag M2 (WB 1:25000, Sigma, F1804), rabbit anti-Tubulin (WB 1:4000 ...
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bioRxiv - Plant Biology 2020Quote: ... They were next incubated in primary antibody solution for 2 hours at room temperature (1:1000 anti-Strep, IBA Life Sciences, 1:10,000 anti-FLAG M2 monoclonal antibody, Sigma in 2% milk Tris – buffered saline ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated with 1:10,000 vol vol−1 secondary antibody anti-mouse horseradish peroxidase (HRP) conjugate (A5278, Sigma–Aldrich, USA) in 2% weight vol−1 BSA-TTBS ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST 1X at RT for 1 hour followed by incubation at 4°C overnight with primary antibodies (SYNGAP-1:2K, PA1-046, Thermofisher; b-Actin-1:5K, A2228, Sigma Aldrich). Membranes were washed thrice with TBST (0.1% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were lysed in 5X Laemmli buffer and the lysates were subjected to SDS-PAGE followed by immunoblotting as previously described using an 1/10000 diluted anti-TEM rabbit serum and an 1/10000 diluted anti-actin antibodies (Sigma-Aldricht).
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling; mouse monoclonal anti-NeuN- Millipore) diluted in a 0.1 % BSA and 0.125 % Triton X-100 solution ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with primary antibodies (rabbit anti-UCP1, 1:750, Abcam ab10983, mouse anti-tyrosine hydroxylase, 1:1,500, Sigma-Aldrich T1299) in blocking buffer overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... brain slices were blocked in PBS-BTA (4% BSA+ 0.25% TritonX100+ 0.02% sodium azide prepared in 1XPBS) for 1 hr followed by incubation with primary antibody-anti-Parvalbumin (1:1000, P3088 Sigma-Aldrich) at RT for 4 hrs with gentle shaking ...
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bioRxiv - Physiology 2021Quote: ... Sections were subsequently incubated with the following primary antibodies for 1 h at room temperature: polyclonal rabbit anti-laminin IgG (L9393, 1:700; Sigma-Aldrich), monoclonal mouse anti-MHCI IgG2b (BA-F8 ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were washed and incubated for 1 h at room temperature with primary antibody (anti-HA, mouse, 1:500, Sigma-Aldrich) diluted in PBS containing 1 % bovine serum albumin ...
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bioRxiv - Cell Biology 2021Quote: ... sections were washed, incubated with a goat anti-rabbit 488 secondary antibody (1:250, Laboratory) and Hoechst (1:500, Sigma-Aldrich) in PBS for 45 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were dried for 10 minutes and then incubated with primary antibody (RPA2 at 1:200, ab2175, Abcam Inc.; γ-H2AX at 1:1000, 05-636, Millipore) in blocking buffer overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunostaining was performed as described previously(45) with the following antibodies: guinea pig α-insulin (1:2000, Millipore/1:800, DAKO), mouse α-glucagon (1:2000 ...
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bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530; Merck Millipore) in blocking solution ...
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bioRxiv - Neuroscience 2021Quote: ... in TBST for 1 h at room temperature (RT) and incubated with primary antibodies against β-actin (mouse, 1:50,000, Sigma, A5316) and Myosin Light Chain 2 (rabbit ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were incubated at room temperature overnight in PBS/ 0.5% Triton X-100/ 1% donkey serum with a goat polyclonal ChAT antibody (1:500 dilution, Chemicon Millipore, AB 144P). After three PBS washes ...
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bioRxiv - Neuroscience 2021Quote: ... plus 5% normal goat serum for 1 hr at RT before 3 d incubation in primary antibody diluted in block (1:1,000, chicken anti-GFP, Abcam, ab13970; 1:200 rabbit anti-cleaved caspase 3, EMD Millipore, AB3623). Sections were washed several times in TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibody diluted in blocking solution (PBST, 1% BSA) overnight at 4°C with the following primary antibodies: mouse anti-NeuN (1:500, Millipore, Mab377), rat anti-BCL11B (1:1,000 ...
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bioRxiv - Cancer Biology 2019Quote: ... overnight at 4°C and followed by 1-h incubation at room temperature with HRP-conjugated anti-rabbit secondary antibody (1:2000, Sigma Aldrich). Chemiluminescence was detected using ECL Western Blotting substrate (Merck ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were incubated at room temperature for 1 h with Cy2/Cy3-conjugated anti-mouse or anti-rabbit secondary antibody (1:50; Millipore AP182C). After three washes in PBS ...
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bioRxiv - Genomics 2019Quote: ... Expressed TUG1 protein was stained for 1 hour at room temperature using a monoclonal anti-FLAG mouse antibody (1:500, F1804, Sigma Aldrich) and co-stained with organelle markers for mitochondria (1:1000 ...
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bioRxiv - Genetics 2020Quote: ... 450 µL of the cleared lysates were incubated with 1 µL of primary antibody (α-Flag M2 (Sigma-Aldrich; 1:400)) at 4 °C for 3 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-α-tubulin (T6074, batch number 023M4813. 1/40000), and anti-HA (H6908, batch number 115M4872v. 1/1000) antibodies were purchased from Sigma-Aldrich. Anti-aurora A (30925 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were then washed with TBST buffer (5 x 1 min) and probed with secondary goat anti-rabbit antibodies conjugated with peroxidase (1:1000, Sigma #A6154). Blots were washed as described above and detection was carried out using Amersham ECL Western Blotting Select Detection Reagent (GE Lifescences #RPN2235 ...
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bioRxiv - Microbiology 2020Quote: ... the plates were washed and incubated with an anti-IpaA (dilution 1/2000e) polyclonal primary antibody3 or anti-vinculin (dilution 1/2000e) Vin11Vin.5 monoclonal antibody (Sigma-Aldrich) in binding buffer containing 0.2% BSA for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... after several washes in PBS explants were incubated at room temperature for 1 hour in secondary antibodies diluted in the blocking solution (at 1:500 for Alexa-coupled antibodies (Life Sciences)) and 1:400 for TRITC-conjugated phalloidin (Sigma-Aldrich)) and then mounted using Fluoromount-G (Southern Biotech).
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bioRxiv - Developmental Biology 2020Quote: ... Every primary antibody was pooled with either α-actinin (1/12,500, sc-17829, Santa Cruz or 1/7000, A7811, Sigma-Aldrich) or α-tubulin (1/6666 ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 hour and incubated overnight at 4°C with primary antibody (ATRX: 1:500, H-300 Santa Cruz Biotechnology Inc.; INCENP: 1:3000, Sigma). The following day the membrane was washed and incubated in the appropriate horseradish peroxidase-conjugated secondary antibody (1:4000 ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Primary antibodies (rabbit anti-phospho-ERK, Cell Signaling Technology #9101, 1:1000; mouse anti-α-tubulin, Sigma-Aldrich #T9026, 1:5000) were diluted in Odyssey Blocking Buffer containing 0.2% Tween 20 ...
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bioRxiv - Microbiology 2019Quote: ... alkaline phosphatase-conjugated (1:10,000) or goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated (1:10,000) secondary antibody (Sigma, St. Louis, MO). Immunoreactive bands were detected by the addition of NBT/BCIP (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1h and probed with the following primary antibodies: guinea-pig anti-Oddskipped (1/100, in house) and mouse anti-actin-HRP (1/20 000, Sigma A3854). After incubation with HRP-conjugated anti-guinea-pig secondary antibody (1/10 000 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... seminase(1:1000) [10,20,40] and mouse antibody against actin (as a loading control; Millipore Corp., cat no. #MAB1501MI at 1:3000) were used as primary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... and the rabbit polyclonal antibody against AF9 (HPA001824, 1:1000 for IB, 1:100 for ChIP) were purchased from Sigma-Aldrich. Antibody against HA (#3724 ...