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Citations for Millipore Sigma :
4601 - 4650 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... the fixed samples were lysed (lysis buffer: 10 mM Tris-HCl pH 8, 10 mM NaCl, 0.3% Igepal CA-630 (Sigma-Aldrich, I8896) and 1× protease inhibitor cocktail (Complete ...
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bioRxiv - Immunology 2021Quote: ... Cultured BMDMs were stimulated with heat-killed KP (MOI = 10) up to 8 h or Klebsiella LPS (1 ug/ml) (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... then 5×106 PFUs were resolved on an 8-12% SDS-PAGE gel and transferred to an Immobilon-FL polyvinylidene difluoride membrane (Millipore, IPEL00010). Membranes were probed for 30 min at room temperature or 4°C overnight with antibodies as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were passaged up to 8 generation post-transfection to eliminate the extrachromosomal donor vector and were then grown under puromycin (Sigma-Aldrich) selection (1μg/ml ...
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bioRxiv - Genomics 2019Quote: ... Wells were transduced with a lentiviral pool expressing the puromycin resistance gene from the Ef1α promoter in the presence of 8 µg/mL polybrene (Millipore Sigma) at a MOI of 1 ...
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bioRxiv - Genomics 2019Quote: Two pools of 150,000 K562 cells without Cas9 were transduced with a pool of lentivirus expressing the Puromycin resistance gene from the Ef1α promoter in the presence of 8 µg/mL polybrene (Millipore Sigma) one at an MOI of 1 and the other at an MOI of 5 ...
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bioRxiv - Genomics 2021Quote: ... Then the mixture was incubated at 37°C for 16 hours by adding 100µg of Ampicillin (Serva, 69-52-3) and 50µg of sodium azide (Merck Millipore,26628-22-8). After the enzyme digestion ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was discarded and the resulting pellet was resuspended with wash buffer (20 mM Tris pH 8, 150 mM NH4Cl, 20 mM MgCl2 supplemented with 1X Cell Lytic B (Sigma-Aldrich). The sample was then spun at 4,000 x g at 4°C for 10 min followed by removing the supernatant and resuspension of the pellet in 4 mL of 50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... The pellet was then resuspended in 330 μL Sorbitol buffer and 192 μL of this suspension were mixed with 8 μL of 50 mg/ml 100T Zymolase (2 mg/ml final concentration; Sigma-Aldrich) and incubated for 5 minutes at 30 °C ...
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bioRxiv - Immunology 2021Quote: ... DCs (3 days post-bone marrow extraction) were transduced by adding lentiviral supernatants in the presence of DEAE dextran (8 µg/ml; Sigma-Aldrich) to cells for 4 h ...
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bioRxiv - Developmental Biology 2020Quote: ... the media was replaced with 8 μL of 100x concentrated library lentivirus mixed in 1 mL ESC medium with 1x polybrene (EMD Millipore). 16 h later ...
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bioRxiv - Immunology 2021Quote: ... A375 and 786O-NL cells by spin-infection (500 g for 2 hours at 37°C) with 8 μg/mL polybrene (Sigma-Aldrich). Four days post-transduction ...
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bioRxiv - Cell Biology 2021Quote: ... The covalently fluorescently labelled nucleosome arrays used for microinjection were labeled at an 8% fluorophore density (8 in 100 histone2B proteins labeled with a fluorophore) and dialyzed against TE (10 mM Tris-HCl (Sigma-Aldrich), pH 7.4 (Sigma-Aldrich) ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: We performed a reverse transduction of both HaCaT and A549 cells by adding lentiviral pHR-SFFV-dCas9-BFP-KRAB or pXPR_120 together with 8 μg/ml polybrene (Sigma-Aldrich, H9268) in DMEM ...
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bioRxiv - Genetics 2021Quote: ... cells used for immunocytochemistry were seeded at 5-8 x 104 cells per cm2 onto poly-D-lysine (PDL) (Sigma, P7405) and laminin-coated 12 mm glass coverslips in 24-well plates or PDL and laminin-coated clear bottom imaging 96-well plates ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were processed and injected into mice ovary orthotopically (n=8) followed by Doxycycline (2mg/ml) and 10% (w/v) sucrose (Sigma-Aldrich) in drinking water after 1 weeks of cells inoculation ...
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bioRxiv - Molecular Biology 2022Quote: ... Genotypes were verified by PCR of tail DNA using the RedExtract-N-Amp Tissue PCR Kit (Sigma Aldrich 254-457-8).
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bioRxiv - Biochemistry 2022Quote: ... mixed 1:1 with fresh medium and added to the desired cells together with 8 μg/ml Polybrene (Sigma, TR-1003).
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bioRxiv - Cancer Biology 2022Quote: ... were applied to PANC-1 cells in the presence of polybrene (8 µg/ml, Cat. no. TR-1003-G, Sigma-Aldrich) for 48 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Packaged lentiviruses were then applied to PANC-1 cells in the presence of polybrene (8 µg/ml, TR-1003-G, Sigma-Aldrich) and incubated over- night ...
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bioRxiv - Bioengineering 2022Quote: ... Filtered particles were either immediately introduced to cells (105 target cells plated in a 6-well plate) in antibiotic-free culture medium supplemented with 8 μg ml-1 polybrene (TR-1003-G, EMD Millipore) or stored in single use aliquots at −80ºC ...
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bioRxiv - Bioengineering 2022Quote: ... containing guide rail shims made from cured SU-8 2025 photoresist (NC9981681, Kayaku Advanced Materials) and rendered hydrophobic by vapor deposition of ∼1 ml dichlorodimethylsilane (DCDMS, 44072, Sigma-Aldrich) in vacuo for ∼10 mins ...
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bioRxiv - Bioengineering 2022Quote: ... Finland) uMp-RW3 micromanipulator was used to control the patch pipette (resistance 4-8 MΩ) containing Ames’ solution (A1420, Sigma Aldrich) buffered with 10mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... NaCl and indium titanium oxide (ITO) coated glass slides (surface resistivity 8–12 V sq-1) were purchased from Sigma-Aldrich Co ...
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bioRxiv - Cell Biology 2022Quote: ... and GST proteins were eluted in GST elution buffer (50 mM Tris-HCl pH 8, 10 mM glutathione (Sigma-Aldrich; G4251). The supernatant was separated from the resin to a fresh tube ...
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bioRxiv - Developmental Biology 2022Quote: ... The pellets were resuspended in aCSF and transferred to Eppendorf tubes pre-coated with 30% BSA (Sigma-Aldrich, 9048-46-8). After cell counting ...
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bioRxiv - Neuroscience 2022Quote: ... the samples were incubated in 1:30 rat anti-DN-cadherin (DN-EX #8, Developmental Studies Hybridoma Bank) and 1:500 rabbit anti-GABA (A2052, Sigma-Aldrich) or 1:200 rabbit anti-Lucifer yellow (A5750 ...
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bioRxiv - Microbiology 2022Quote: ... cells seeded in 12-well plates were pre-treated with 500 mU/mL α2-3/6/8 sialidase from Clostridium perfringens (Sigma-Aldrich) for 3 hours at 37°C before infection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Ear biopsies of mice 4–8 weeks old were incubated at 55°C for overnight in lysis buffer containing 75 mM NaCl (SIGMA, S9888), 25 mM EDTA (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... Stock cultures were stored at -80°C in cryogenic vials by resuspension of fresh growth in Tryptic Soya Broth (TSB; Oxoid) containing 8% (v/v) dimethyl sulfoxide (Sigma Aldrich). Culture purity was determined by plating frozen stocks onto Tryptic Soy Agar (TSA ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μL lysate was mixed with 45 μL MUG (methylumbelliferyl b-D-glucuronide) solution (10 mM Tris/HCl pH 8, 2 mM MgCl2, 1 mM MUG (Sigma Aldrich)) in a 96-well plate ...
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bioRxiv - Genomics 2022Quote: ... K562 or Jurkat cell lines expressing CRISPRi effectors of interest were infected with lentivirus containing sgRNA expression vectors by centrifugation at 1000 × g for 1 h in 24-well plates in the presence of 8 µg/mL polybrene (Sigma-Aldrich). RPE1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysates were sonicated on ice with a microtip sonicator (120 W, 8 s on, 2 s off, 3 pulses) and supplemented with 200 U of benzonase (Sigma; E8263). The lysates were cleared by centrifugation (18000 g ...
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bioRxiv - Molecular Biology 2022Quote: ... were grown in TYG medium (with/without kanamycin; 8 µg/ml) in 96 well microtiter plates after washing with PBS (Nunclon; Sigma-Aldrich). Optical density at 600nm was measured to examine the growth in replicates at 32 °C for 18 h using Synergy H1 Hybrid multi-mode microplate reader ...
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bioRxiv - Molecular Biology 2022Quote: ... at a constant flow rate of 0.8 ml/min and a constant temperature of 22°C with mobile phase A being 0.4 % acetic acid (Sigma-Aldrich, USA) in water and phase B being 20% Methanol (Honeywell ...
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bioRxiv - Cancer Biology 2022Quote: LLC cells were treated with conditioned media from inflammasome-induced macrophages as indicated and 5 × 104 cells per well were seeded in top chambers of the transwell chamber (8 μM pore, 24-well plate, Millipore-Sigma) in FBS-free media with membrane inserts ...
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bioRxiv - Cell Biology 2022Quote: ... filtered through 0.22 μm filter and used to infect HUVECs (passage 2) in the presence of polybrene (8 μg/ml, H9268; Sigma-Aldrich). The transduced HUVECs were selected with puromycin (8ug/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... The 1ml of blood was split into two flasks with 30ml of media added to each and delayed until 8 pm on Wednesday at which point dimethyl sulfoxide (DMSO) was added to one flask and rapamycin (Sigma-Aldrich) resuspended in DMSO added to the other at a 1:10,000-fold dilution to give a final rapamycin concentration of 10nM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Stock solutions of CuSO4 (39 mg/L) were prepared by dissolving CuSO4·5H2O (CAS# 7758-99-8; purchased from Sigma-Aldrich) in distilled water ...
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bioRxiv - Cancer Biology 2024Quote: ... The uncured SU-8 was washed away using SU-8 developer and the wafer was silanized using Trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma 448931). Sylgard 184 (Ellsworth ...
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bioRxiv - Cancer Biology 2023Quote: ... and then directly added to the intended cells in the presence of 8-10 µg/ml polybrene (TR-1003-G, Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... Demyelination was induced in 8-week-old male mice via a 0.2% w/w cuprizone diet (Sigma-Aldrich, cat# C9012-25G). The diet was freshly prepared by mixing cuprizone with standard powder food and water ...
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bioRxiv - Developmental Biology 2024Quote: ... hPSC were seeded and aggregated in EB formation medium (Essential 8 containing 400 µg/mL Poly (vinil alcohol) (Sigma Aldrich, P8136) and 10 µM Y-27632 (Bio-Connect ...
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bioRxiv - Cell Biology 2024Quote: ... Equal quantities of proteins (20 μg/lane) were loaded onto 8% or 10% SDS-PAGE gels and subsequently transferred to PVDF membranes (Millipore, USA). The membranes were then incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... cells were infected at an estimated MOI of about 1 for each lentivirus in the presence of 8 µg/ml polybrene (EMD Millipore) in 1.5 ml inoculum and incubated at 37 °C for 2 h rocking and rotating every 10 min before adding 8.5 ml of 5 % FBS/DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... 3T3-L1 fibroblasts were transduced with titrated viruses in presence of 8 µg/mL of polybrene (Hexadimethrine bromide, Cat. No: 107689-10G, Sigma-ALDRICH) and subjected to antibiotic selection using 4 µg/mL puromycin after 48 hours ...
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bioRxiv - Pathology 2024Quote: Nuclei from frozen mouse kidney outer cortex tissue samples and glomeruli-enriched samples were prepared at 4°C.40 Tissue fragments (∼ 8 mm3) were cut by razor blades in EZlysis buffer (#NUC101-1KT, Sigma-Aldrich) and homogenized 30 times using a loose Dounce homogenizer and 5 times by tight pestle ...
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bioRxiv - Genetics 2023Quote: ... Experimental Villin-CreERT2 Hnf4 mutant mice (8–12 weeks old) were intraperitoneally administered tamoxifen at 50 mg/kg/day (Sigma, T5648). Wild-type ...
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bioRxiv - Cell Biology 2023Quote: ... elegans genomic DNA extracted from 8-10 adult worms using the Extract-N-Amp™ Tissue PCR Kit (Millipore Sigma, MA) as previously described38 then sequenced ...
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bioRxiv - Genomics 2023Quote: ... Samples were separated on SDS-PAGE (stacking 4% and resolving (8% top and 15% bottom)) and the protein-resolved gel was transferred onto a PVDF membrane (Millipore, #IPVH00010) in a 1X transfer buffer (25 mM Tris ...