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Citations for Millipore Sigma :
4601 - 4650 of 10000+ citations for 6 Ethyl 1H indole 2 3 dione 3 O 4 4 4 trifluoro butyl oxime since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 0.3% TritonX/PBS) for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I ...
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bioRxiv - Developmental Biology 2019Quote: 2-[4-(Dimethylamino)styryl]-1-ethylpyridinium iodide (DASPEI) labeling in live fish was performed by diluting 1mM DASPEI (Sigma-Aldrich) in system water ...
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bioRxiv - Genomics 2019Quote: Human liver specimens obtained from resections were perfused for 15 minutes with calcium-free 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid buffer containing 0.5 mM ethylene glycol tetraacetic acid (Fluka) followed by perfusion with 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid containing 0.5 mg/mL collagenase (Sigma-Aldrich) and 0.075% CaCl2 at 37°C for 15 min as previously described53 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... The re-suspensions were spread on 7H11 OADC plates supplemented with the relevant drug concentration (2, 4, or 8 µg/mL of ofloxacin, or 100 µg/mL streptomycin; Sigma). Resistant colonies were observed and enumerated after 21 to 35 days of incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentrations of 29 cytokines/chemokines were measured in 2-4 biological replicates using MILLIPLEX Map Human Cytokine/Chemokine Magnetic Bead Panel (HCYTMAG-60K-PX29, #Millipore) according to manufacturer’s protocol.
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bioRxiv - Plant Biology 2020Quote: ... stratified for 2 d at 4°C in the dark and sown on Gamborg’s B5 Basal Salt medium (Sigma G5768) supplemented with 1% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... 4°C) and resuspended in binding buffer (50 mM Tris, 1 mM MgCl2, 2 mM imidazole, 1 mM PMSF (Sigma), 300 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Microbiology 2019Quote: ... and media with 4 mM Ca2+ was made by adding 2 mM CaCl2 to the standard high-glucose DMEM (Sigma). We maintained TV-infected cells under these conditions until the positive control (normal media ...
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bioRxiv - Developmental Biology 2019Quote: 6 dpf Cntnap2a-positive fish were treated for 1 hour with 0.05% DASPEI (2-4-dimethylamino-N-ethylpyridinium iodide; Sigma Aldrich), which labels both superficial and canal neuromasts [46] ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... at 25,000 x g for 2 h at 4°C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were discarded ...
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bioRxiv - Biochemistry 2021Quote: ... The second half of the clarified lysate was incubated for 2 h at 4°C with 20 μl of Anti-FLAG M2 magnetic beads (Sigma) equilibrated in FLAG wash buffer containing 20 mM HEPES pH 8.0 ...
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bioRxiv - Pathology 2021Quote: ... at 25,000 x g for 2 hours at 4 °C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were decanted ...
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bioRxiv - Neuroscience 2021Quote: ... Following electrophoresis, proteins were transferred (100 V, 2 hr, 4°C) to polyvinylidene difluoride membranes (Immobilon-FL, Millipore, Billerica, MA). To confirm protein transfer ...
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bioRxiv - Developmental Biology 2022Quote: ... Each sample was incubated with primary antibodies for 1–2 h at room temperature or overnight at 4°C and then with fluorescent-conjugated secondary antibodies and DAPI (Sigma) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... femurs and tibias of C57BL/6J mice were extracted and crushed on a mortar in PBS supplemented with 4%FBS and 2 mM EDTA and filtered through 0.45μm strainers (Merck Millipore, Cat#SLHV033RB). For B cells ...
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bioRxiv - Biochemistry 2022Quote: ... and cell pellets were collected and resuspended in the resuspension buffer containing 20 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma) pH 7.4 and 200 mM NaCl (Sigma)] ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μg of total protein from PC12 cell extracts were incubated over night at 4°C with 2 μg of the phospho- tyrosine 4G10 (Sigma-Alrich/Upstate Cat# 05-321 ...
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bioRxiv - Neuroscience 2022Quote: ... Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM, Sigma-Aldrich, P8340) and was added to each frozen brain section on ice ...
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bioRxiv - Molecular Biology 2022Quote: 400 μl of cell lysate was incubated overnight at 4 °C with 2 μg of anti-Myc or anti-Flag antibody (Sigma). Protein A-Sepharose (GE ...
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bioRxiv - Cancer Biology 2024Quote: ... 80 μM aprotinin, 4 mM bestatin, 1.4 mM E-64, 2 mM leupeptin and 1.5 mM pepstatin A; Sigma-Aldrich, P8340) and 50 nM calyculin A (LC Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM, Sigma-Aldrich, P8340) and was added to each frozen brain section on ice ...
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bioRxiv - Neuroscience 2024Quote: ... Media was incubated at 4 °C for 2 hours in 20% PEG solution (40% w/v stock: 2.5 M NaCl, 40% PEG (Sigma P2139) followed by a 20 min 3,000 x g spin at 4 °C ...
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Physical activity increases neuronal activity in the circadian clock of diurnal Arvicanthis ansorgeibioRxiv - Neuroscience 2024Quote: ... The brain was then removed and fixed for 2 days in 4% paraformaldehyde containing C6FeK4N6·3H20 (Sigma-Aldrich, Lyon, France). Coronal slices (40 µm thick ...
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bioRxiv - Developmental Biology 2023Quote: We scanned formalin-fixed whole kidneys stained for 2 hours in 4% OsO4 solution (Sigma-Aldrich, St. Louis, MO, USA) using a previously developed method 31 ...
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PQS and Pyochelin in Pseudomonas aeruginosa Share Inner Membrane Transporters to Mediate Iron UptakebioRxiv - Microbiology 2023Quote: ... A total of 100 µL of 4 mg/mL 2- nitrophenyl β-D-galactopyranoside (ONPG) (Sigma, St. Louis, MO, USA) was added to the cells ...
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bioRxiv - Molecular Biology 2023Quote: The feeder-dependent murine ESC line v6.5 and feeder free Bruce-4 cells were cultured in 2% gelatin-coated (SIGMA, G1393) ventilated-cap flasks ...
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bioRxiv - Cell Biology 2023Quote: ... adipocytes were lysed in polysome lysis buffer (20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl2, 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma] ...
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bioRxiv - Cell Biology 2023Quote: ... Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich, cat.no. 59427C-100ML). HEK293T-LentiX (Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... sulfo-succinimidyl-4-(N-maleimidomethyl)cyclohexane-l-carboxylate (sulfo-SMCC, Sigma, at a concentration of 2 mg/mL in PBS) were added to the emulsion and kept to react for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was concentrated using ultracentrifugation in an SW-28 rotor (25,000 rpm, 2 h at 4°C) on a 60% OptiPrep density cushion (Sigma-Aldrich). The pelleted viruses were resuspended in 10 mL TNE buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... cells were centrifuged with 293T retroviral supernatant at 1000×g for 2 hr at 37°C in the presence of 4 µg/mL polybrene (Sigma). Following spin infection ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was clarified by centrifugation and incubated for 2 hours at 4 °C with anti-FLAG M2 agarose beads (Sigma) with gentle rotation ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then washed 3 times for 20 - 40 minutes each wash in PBS before incubation with secondary antibody(s) in blocking solution for 2 - 4 hours at room temperature (Alexa 647-conjugated donkey anti-chicken, AP194SA6, Millipore – to label GFP ...
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bioRxiv - Immunology 2024Quote: ... Next, 50 μl of 2× STOP buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA (cat. E3889, Sigma-Aldrich) and 100 μg/ml RNase A (cat ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... flies were homogenized in 50 μl PBS per fly (between 2-4 flies per sample) containing 0.1% Tween-20 (Sigma #1379) using a TissueLyser LT (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with 2% (74.4 mOsmol/kg) or 4% PEG-300 (148.8 mOsmol/kg; v/v; Millipore Sigma 8074845000) in culture medium for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: MM-NLP particles isolated from both sucrose gradient sedimentation (native) and GraFix (crosslinked) were concentrated to 2 µM using Amicon Ultra-4 centrifugal filters (Ultracel 30K, Millipore). C-Flat 1.2/1.3 (Cu ...
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bioRxiv - Genetics 2020Quote: ... 3 mg of each compound was dissolved in 6 mL hexane (Sigma-Aldrich #139386-500ML) and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab ...
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bioRxiv - Biochemistry 2020Quote: ... The complex was then concentrated to 3-6 mg/ml (Amicon 100 kDa cutoff, Millipore) and loaded onto a size-exclusion column equilibrated with 20 mM HEPES ...
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bioRxiv - Genetics 2022Quote: ... 3 mg of each compound was dissolved in 6 mL hexane (Sigma-Aldrich #139386-500ML) and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab ...
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bioRxiv - Developmental Biology 2022Quote: ... inflorescences from 3-week-old plants were treated with 5 μM 6-BA (Sigma-Aldrich), 50 μM IAA (Sigma-Aldrich) ...
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bioRxiv - Physiology 2021Quote: ... 6 corneas from 3 individuals were pooled in 100 μl of Tri reagent (T9424, Sigma). RNA was isolated from TRI reagent (T9424 ...
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bioRxiv - Immunology 2023Quote: ... the peroxidase substrate ABTS (2,2’-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt, Sigma, A1888) was added and the absorbance determined at 405nm ...
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bioRxiv - Genetics 2023Quote: ISE6 cells (3×106 cells per well) were plated in 6-well plates (Millipore Sigma) and stimulated accordingly ...
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bioRxiv - Biochemistry 2024Quote: ... N-(3-oxododecanoyl)-L-homoserine lactone (AHL-C12) and 6-methoxyflavanone were purchased from Sigma. Stock solutions of these compounds (DPH ...
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bioRxiv - Microbiology 2024Quote: ... L-Tryptophan- (indole-d5) (Sigma Aldrich), Hippuric Acid-[13C6] (IsoSciences) ...
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bioRxiv - Microbiology 2024Quote: ... L-Tryptophan-(indole-d5) (Sigma Aldrich), Hippuric Acid-[13C6] (IsoSciences) ...