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Citations for Millipore Sigma :
4551 - 4600 of 10000+ citations for 6 Ethyl 1H indole 2 3 dione 3 O 4 4 4 trifluoro butyl oxime since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Assessment of the transcriptional regulation of EMT and MET through accessible transposable elementsbioRxiv - Genomics 2024Quote: Cells were washed with PBS and fixed with 4% formaldehyde (Sigma) for 10 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... chicken type II collagen (CII) (4 mg/ml CII, Sigma, USA) was emulsified in complete Freund’s adjuvant consisting of 10 mL of Freund’s incomplete adjuvant plus 40 mg of Mycobacterium tuberculosis H37RA ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μl of casein solution (0.2 mg/ml, Sigma-Aldrich C3400) and 8 μl of PBS pH 7.4 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed in freshly prepared 4% paraformaldehyde (Sigma-Aldrich, P6148) overnight at 4°C and dehydrated into 100% methanol (VWR ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were embedded in 4% low melting temperature agar (Sigma-Aldrich) and sectioned at 50 μm on a vibrating microtome (Leica) ...
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bioRxiv - Immunology 2024Quote: 4 mm biopsy of skin samples were stored in RNAlater (Sigma) at -20°C ...
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bioRxiv - Developmental Biology 2024Quote: ... 4 µM TRULI (MedChem Express HY-138489 or Sigma-Aldrich SML3634), or equivolume DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... The coverslips were fixed in 4% paraformaldehyde (PFA; Sigma, cat. #47608) in PBS for 12 min either at room temperature or at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... 4 μl/ml of human fibronectin (1 mg/ml; Sigma-Aldrich), and 10 μl of 1 N NaOH (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... cells were boosted with D-glucose (4 g/L, Sigma-Aldrich) and valproic acid (3 mM ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared and stained embryos were transferred into ethyl cinnamate (Sigma-Aldrich, #103-36-6). Light sheet imaging was performed using a Zeiss Z.1 Light sheet microscope equipped with a 5x/0.16 detection objective ...
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bioRxiv - Synthetic Biology 2020Quote: ... and blocked for 1h in 3% BSA (Fisher bioreagents CAS 9048-46-8) and 0.1% Triton X-100 (Sigma 9002-93-1). The cells were then incubated in primary antibody overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Fixation was followed by blocking (1h, RT) with 3% bovine serum albumin (BSA/0.01% Tween™20/PBS) (Sigma, Cat#A-7906). Samples were incubated with primary antibodies (overnight ...
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bioRxiv - Evolutionary Biology 2021Quote: ... ethyl decanoate and ethyl dodecanoate (all Sigma Aldrich). 2-Methoxy-3-methyl-1,4-BQ was synthesized as follows ...
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bioRxiv - Developmental Biology 2020Quote: Tadpoles were anesthetized at room temperature by incubation for 30-60 seconds in a 1:2000 dilution of MS222 (ethyl 3-aminobenzoate methanesulfonate, Sigma, St. Louis, MO) diluted in 1/20X Normal Amphibian Media ...
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bioRxiv - Molecular Biology 2022Quote: Embryos at stage 29-31E (before the pre-hatching period) were anesthetized with 0.02% ethyl 3-aminobenzoate methanesulfonate (MS-222) (Sigma-Aldrich, St. Louis, MO) in natural seawater and sacrificed by decapitation ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing PelXC232S were pooled and concentrated to a volume of 2 mL by centrifugation at 2 200 × g at a temperature of 4 °C using an Amicon ultra centrifugal filter device (Millipore) with a 10 kDa molecular weight cut-off ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed on 2 mg of total protein by incubating 2h at 4°C with anti-Flag agarose beads (Sigma). Beads were then washed in lysis buffer and bound polypeptides analyzed by SDS-PAGE and immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... NRCMs with serum-free DMEM no glucose media and ARCMs with serum-free media 199 for 16 h before treating with 4-methyl 2-oxopentanoic acid sodium salt (ketoleucine, Sigma), sodium-3-methyl-2-oxobutyrate (ketovaline ...
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bioRxiv - Immunology 2021Quote: ... the supernatant media was removed and replaced with 4 ml of digestion media consisting of DMEM with 2% FBS (Sigma), with 100ug/ml DNase (Roche ...
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bioRxiv - Immunology 2021Quote: Three- to 4-week-old mice were injected intraperitoneally twice with 0.25 mg 5-ethynyl-2’-deoxyuridine (EdU; Sigma-Aldrich) 2 hours apart ...
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bioRxiv - Developmental Biology 2020Quote: ... The female mice were sacrificed by cervical dislocation and the whole female reproductive tract with the inner contents was collected with the copulation plug at 1 hour or 2 hours after coitus and placed into 4% paraformaldehyde (PFA, Sigma) at 4°C overnight.
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bioRxiv - Molecular Biology 2022Quote: ... in a 10-cm dish transfected one day post seeding with packaging plasmids (1 μg VSV.G, 2 μg psPAX2 and 4 μg of transfer plasmid using PEI (Sigma-Aldrich). Virus containing supernatant was collected 72 hours post transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were washed once with 40 mL 0.1 mM 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF, Sigma-Aldrich A8456), transferred into conical 50 mL tubes and centrifuged at 1700 x g for 5 min ...
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bioRxiv - Biophysics 2022Quote: ... of polyethylene glycol (PEG) of a range (0.4, 2, 4, 8, 20 or 35 kDa) of molecular weights (Sigma-Aldrich). GC376 (GC376 Sodium ...
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bioRxiv - Immunology 2020Quote: ... siLP cells were released by digestion of the tissue with RPMI/4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) supplemented with 60 μg/ml DNaseI (Sigma), and 400 ng/ml of Liberase (Roche Applied Science ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... wells were washed with embryo media before the fish were incubated in media containing 0.01% 2-[4-(dimethylamino) styryl]-1-ethylpyridinium iodide (DASPEI, Sigma-Aldrich) to stain for neuromast mitochondrial activity for 20 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
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bioRxiv - Physiology 2021Quote: ... cells were treated with poly(I:C) (1.5 μg/mL for 4 hrs) and a combination of proteasome inhibitor (Bortezomib, 2 μM, Sigma/Calbiochem 5043140001) and prolyl endopeptidase inhibitors (Pramiracetam 10 uM ...
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bioRxiv - Genetics 2021Quote: ... and 0.1% (w/v) SDS) containing 1× homemade protease inhibitor cocktail (1 mM 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (Sigma; A8456), 0.3 μM Aprotinin ...
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bioRxiv - Genetics 2020Quote: ... An aliquot of 2 µl sperm HTF suspension was mixed immediately with 50 µl of 4% PVP (Sigma, Cat# P5288) in water (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was re-suspended in ice-cold 100 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid) buffer (pH 8.0) containing complete protease inhibitor cocktail (Sigma, USA) and PMSF (phenylmethanesulphonyl fluoride ...
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bioRxiv - Neuroscience 2020Quote: ... 10 mm petri dish and fixed for 10 min in 4% formaldehyde (Mecalab) diluted in PBS (in mM: 137 NaCl, 10 Na2HPO4, 2.7 KCl, 2 KH2PO4; all Sigma-Aldrich) at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:1 mixture of 4% HMS (2% final concentration) and 20% bovine serum albumin (BSA; 10% final concentration; V900933, Sigma), incubated at 4°C for 1 week ...
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bioRxiv - Cancer Biology 2021Quote: 105 HCT116 cells as well as HCT116 cells with acquired oxaliplatin resistance and HCT116 cells with BOLD-100/KP1339 resistance were seeded (N = 4 for each respectively) into 12-well plate (StarLab) with 2 mL McCoy’s 5A medium (Sigma-Aldrich) containing 10% FCS (BioWest ...
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bioRxiv - Biophysics 2020Quote: ... The virus-containing supernatant was concentrated by ultracentrifugation in a SW-28 rotor (25,000 rpm, 2 h, 4°C) using OptiPrep density gradient medium (Sigma-Aldrich). Virus containing fractions were collected ...
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bioRxiv - Cell Biology 2020Quote: ... For AsiSI or I-PpoI nuclease induced DSBs, cells were treated with Shield-1 (1 μM, AOB1848, Aobious) and 4-OHT (2 μM, H7904, Sigma) for 5 hours before washing with PBS and adding back media.
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Plant Biology 2021Quote: ... 1 g of the infiltrated leaf tissue was ground in 4 mL of extraction buffer (1/2 tablet of Sigma Complete™ mini protease inhibitor (per 15 mL) ...
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bioRxiv - Microbiology 2021Quote: Fifty microliters containing 3.5 x 106 PFU/ml SARS-CoV-2 viral stock was placed on one well of a 4-well chambered glass slide (Nunc, Sigma) (Fig 2) ...
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bioRxiv - Neuroscience 2020Quote: ... 66 These transgenic mice express the GEVI in cortical layer 2/3 pyramidal neurons under the intersectional control of TetO and destabilised Cre-recombinase.70–72 The destabilised Cre-recombinase was stochastically re-stabilised to induce sparse expression of the voltage indicator via two IP injections of a total of 2 × 10−4 mg kg−1 Trimethoprim (TMP, Sigma) over 2 consecutive days as described in Song et al ...
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bioRxiv - Biophysics 2021Quote: ... The virus-containing supernatant was concentrated by ultracentrifugation in a SW-28 rotor (25,000 rpm, 2 h, 4°C) using OptiPrep density gradient medium (Sigma-Aldrich). Part of the supernatant containing pelleted viruses was collected ...
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bioRxiv - Neuroscience 2022Quote: Headless larvae (7 dpf) were fixed for three days at 4 °C in EM fixation buffer [2% EM-grade glutaraldehyde (Sigma), 2% PFA (Sigma) ...
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bioRxiv - Microbiology 2022Quote: Sialidase activity was fluorometrically monitored using the substrate 2’-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-Neu5Ac; Sigma-Aldrich). Reactions were performed in a final volume of 50 μL ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were equilibrated at 4 °C and incubated for 30 min in the presence of 2 mg/ml iodoacetamide (Sigma). Membranes were then solubilized in 10 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... mice were fasted for 4 hours followed by gavaging of 2 g/kg (10 ml/kg) of D-glucose (Sigma) dissolved in distilled water ...
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bioRxiv - Immunology 2020Quote: ... A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 µg/mL final concentration per peptide) or controls (gp70-AH1 [SPSYVYHQF]38, 4 µg/mL; concanavalin A [ConA], 2 µg/mL [Sigma]). Streptavidin-alkaline phosphatase (ALP ...
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bioRxiv - Immunology 2020Quote: ... A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 μg/mL final concentration per peptide) or controls (gp70-AH1 [SPSYVYHQF]33, 4 μg/mL; Concanavalin A [ConA], 2 μg/mL [Sigma]). Streptavidin-alkaline phosphatase (ALP ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was collected in clean microcentrifuge tube and incubated 2 h at 4°C with a-flag mouse antibody (Sigma), following 1 h incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysate was immunoprecipitated for 2 hours at 4°C with the following antibodies: rabbit anti-myc (2.5 μg, Millipore Sigma), mouse anti-HA (2.5 μg ...