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Citations for Millipore Sigma :
4551 - 4600 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... were sourced from Nottingham Arabidopsis Stock Centre and homozygous mutants where confirmed through genotyping PCR on genomic DNA using REDTaq® ReadyMix™ (Sigma) following their protocol ...
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bioRxiv - Genomics 2021Quote: ... we performed up to 8 nested PCR reactions in parallel with an input of 100-200ng per reaction using the KAPA2G Robust ready mix (Sigma-Aldrich, KK5702) in a volume of 50μL ...
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bioRxiv - Microbiology 2022Quote: ... The amplified PCR products were visualized by 1.5% agarose gel electrophoresis and purified using a Millipore filter plate MSNU030 (Millipore SAS, Molsheim, France). The purified PCR products were then subjected to Sanger sequencing using the BigDye Terminator v3.1 sequencing kit and a 3730xl automated sequencer (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed using either KAPA HIFI 2X ready mix (KAPA Biosystem, cat# KK2602) or Expand Long Template PCR System (Sigma, cat# 11681842001). Primers used for each PCR reaction are listed in Table S4.
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bioRxiv - Cancer Biology 2023Quote: ... through a 130 μm low-pressure deposition nozzle into 96 well PCR plates containing 10 µL 1x prepared Cell Lysis and Fragmentation Buffer Mix (Millipore Sigma, WGA4). Doublets were excluded from analysis via standard FSC/SSC gating procedures ...
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bioRxiv - Molecular Biology 2023Quote: ... The locus with expected insertion was amplified by PCR using 5 μL KOD Hot Start 2× Master Mix (Sigma Aldrich 71842-3), 0.2 μL each primer at 10 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Plant Biology 2021Quote: ... was analyzed by extracting the gDNA with either the innuPREP Plant DNA Kit (Analytik Jena; Jena, Germany; T0) or the REDExtract-N-AMP plant Kit (Sigma Aldrich; T1, T2). The transgene was detected by amplifying the Ocs terminator of the hygromycin resistance cassette (Table S3).
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bioRxiv - Bioengineering 2022Quote: ... the lipid oxidation and superoxide level assays were conducted according to manufacturer’s specifications (Lipid peroxidation assay kit, Sigma Aldrich Cat#MAK085; Fluorimtric hydrogen peroxide assay kit, Sigma Aldrich Cat#MAK165). Total GSH level of liver tissue was measured using a glutathione colorimetric detection kit (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... IL-10 and TNF-α were determined in 24 h culture supernatant of UNT control and treated MOs using respective ELISA kits (Sigma-Aldrich kit, USA), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... PCR reactions were analyzed by gel electrophoresis using a 1% agarose gel in TAE buffer (20 mM acetic acid (Sigma Aldrich, cat#, 695092), 2 mM EDTA (ACROS Organics ...
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bioRxiv - Immunology 2019Quote: ... were designed using the chop-chop website (55) and generated by PCR complementation and amplification of full length ssDNA oligonucleotides (Sigma-Aldrich, Table S3) as described (19) ...
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bioRxiv - Biochemistry 2020Quote: DNA encoding for Pry1 and Na-ASP-2 were PCR amplified and cloned into NcoI and XhoI restriction sites of pET22b vector (Novagen, Merck, Darmstadt, Germany), which contains a pelB signal sequence to direct the secretion of expressed protein into the periplasmic space ...
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bioRxiv - Microbiology 2020Quote: Full-length sequences of selected sdAbs were PCR amplified and cloned into the NcoI/XhoI sites of the pET28b (Novagen, Sacramento, CA, USA) and transformed into BL21(DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... An amount of 50 μg of Co-IP sample was transferred to a PCR tube and incubated with 1 μL of benzonase (Sigma, cat. no. E8263) at 37°C for 30 min to shear and digest DNA ...
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bioRxiv - Microbiology 2021Quote: Full-length sequences of selected nanobodies were PCR amplified and cloned into the NcoI/XhoI sites of the pET28b (Novagen, Sacramento, CA, USA) and transformed into BL21(DE3 ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR products were purified and used for one-step isothermal assembly with EcoRV-digested pFLAG-CMV-5.1 (Sigma, St. Louis, MO, USA)32.
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bioRxiv - Microbiology 2024Quote: ... [22]) were used to amplify the 16S V4 variable region in PCR reactions using Kapa HiFi Hotstart ready mix (Sigma-Aldrich, Oakville, Canada) (Tables S2 ...
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bioRxiv - Cell Biology 2023Quote: ... were generated by PCR and cloned into the pFlag-CMV4 plasmid (for N-terminal Flag fusion in mammalian cells, cat#E7158, Merck Millipore, Burlington, MA, USA). CDS of ARVA ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 100,000 nuclei were sorted at a ratio of 70% NeuN+ and 30% NeuN-into a single well of a PCR plate (Sigma-Aldrich, EP0030129504-25EA) containing 50 µL FANS buffer (see Table S8) ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted (Spectrum™ Plant Total RNA Kit, Sigma) from male and female floral tissues ...
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bioRxiv - Genetics 2020Quote: Telomerase enzymatic activity was analyzed using the TRAPeze kit (Millipore) per manufacturer’s directions ...
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bioRxiv - Microbiology 2020Quote: ... Concentrations of glucose were determined using the GAGO20 kit (Sigma).
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bioRxiv - Systems Biology 2020Quote: ... and the Milliplex 38 Cytokine kit (Millipore, HCYTMAG-60K-PX38).
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bioRxiv - Cell Biology 2020Quote: ... using the Duolink in situ red starter kit (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Gel filtration standards (Gel Filtration Markers Kit, MWGF1000, Sigma Aldrich) were separated on similar gradients to estimate protein complex size and included ...
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bioRxiv - Immunology 2021Quote: ... Triglycerides were measured using Triglyceride quantification Colorimetric/Fluorometric Kit (Sigma). For trehalose quantification ...
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bioRxiv - Immunology 2021Quote: ... αKG was measured using a colorimetric assay kit (Sigma Aldrich). All kits were used according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: CTCF ChIP was conducted using Millipore ChIP agarose kit (Millipore). 1×106 crosslinked 416B cells were lysed and sonicated using a Covaris ultrasonicator ...
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bioRxiv - Physiology 2022Quote: Liver triglycerides were measured using triglyceride determination kit from Sigma (catalog n°TR0100 ...
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bioRxiv - Molecular Biology 2020Quote: ... then subjected to nuclei extraction (Nuclei EZ prep kit, Sigma). Genomic DNA was isolated using DNAzoL reagent (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... High Sensitivity Triglyceride Fluorometric Assay Kit from Sigma (MAK264-1KT) and Phospholipid quantification Assay Kit from Sigma (CS0001-1KT)).
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bioRxiv - Immunology 2022Quote: ... Serum glycerol were measured using glycerol assay kit (Sigma, MAK117) and normalized to the weight of body fat.
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bioRxiv - Systems Biology 2020Quote: ... Colorimetric glycerol assays were acquired using a commercial kit (Sigma) where the provided assay powder was resuspended in 40 mL of distilled H2O ...
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bioRxiv - Physiology 2019Quote: Plasma insulin was determined using a commercial kit (Merck Millipore) on a MAGPIXTM Multiplex reader and processed using Bio-Plex ManagerTM MP.
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bioRxiv - Microbiology 2019Quote: ... the KAPA SYBR FAST Universal qPCR kit (Sigma-Aldrich, Sweden), and primers specific to the conserved gtfB and gtfC regions in a Corbett Rotor-Gene 6000 apparatus [29] (S1 Table) ...
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bioRxiv - Cell Biology 2019Quote: ... Human c- peptide ELISA kit (cat # EZHCP-20K, Millipore MA), Human Insulin ELISA kit (cat # ELH- Insulin ...
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bioRxiv - Cell Biology 2019Quote: Duolink PLA was performed using the Duolink Red Kit (Sigma). In summary ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... TRAP staining was performed with a commercial kit (Sigma-Aldrich), randomly selected 5 fields of view on the pressure side of the mesial buccal root of maxillary orthodontic tooth in rats ...
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bioRxiv - Physiology 2019Quote: ... followed by determining protein concentration using BCA assay kit (SIGMA). Samples were loaded onto SDS-PAGE for protein expression analysis.
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bioRxiv - Developmental Biology 2020Quote: ... Assay was performed as per the kit instruction (DUO92101, Sigma). The antibodies used were AR (Sigma A9853 ...
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... and protein was quantified using a Bradford assay kit (Sigma) following manufacturers’ instructions.
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bioRxiv - Physiology 2020Quote: ... ChIP assays were performed using an EZ-Chip Kit (Millipore). Chromatin lysates were prepared ...
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bioRxiv - Plant Biology 2019Quote: Total RNA was isolated using the RNA isolation kit (Sigma). After RNase-free DNase treatment ...
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bioRxiv - Genetics 2020Quote: ... Proteins were quantified by Bradford Protein Assay Kit (Sigma-Aldrich). Protein samples were separated by 4-12% Bis-Tris Protein Gels (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... were measured using an MDA assay kit (#MAK085, Sigma Aldrich) in hippocampal brain tissue isolated from 23 month old APP/PS1 and NTG mice treated for 14 months with CP2 or vehicle (n = 5 per group) ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were isolated using the NucleiEZ nuclei isolation kit (Sigma), with modifications described in Supplemental Methods ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using the Spectrum Total Plant RNA isolation kit (Sigma-Aldrich), at SNM ...
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bioRxiv - Bioengineering 2020Quote: ... For Picrosirius red staining (Picro Sirius Red Stain Kit, Sigma) we soaked the section for 30 min in distilled water ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were developed with Immobilon Western Chemiluminescence Kit (Millipore, #WBKLS0500).