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Citations for Millipore Sigma :
4401 - 4450 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... T4 ligations into multiple cloning site plasmids were performed by amplifying fragments via PCR with Roche Expand High Fidelity Polymerase (Sigma 11732641001) with primers containing 5’ restriction enzyme sequences for digestion following PCR purification ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial DNA was used to amplify the entire 16S region by PCR [5′-AGAGTTTGATCCTGGCTCAG-3′ (forward) and 5′-AAGGAGGTGATCCAGCCGCA-3′ (reverse)] using Expand High-Fidelity Polymerase (Sigma-Aldrich). The PCR products were purified using the QIAquick PCR Purification kit (Qiagen) ...
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bioRxiv - Synthetic Biology 2023Quote: ... which can be PCR amplified (Phanta Max Super-Fidelity DNA Polymerase, Vazyme) with a set of primers (ordered from Microsynth or Sigma-Aldrich) to add a unique variable linker ...
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bioRxiv - Biochemistry 2023Quote: ... 1 µL of diluted cDNA was mixed with 12,5 µL ReqTaq® Ready Mix™ PCR reaction mix (Sigma Aldrich, #R2523), 0,5 µL forward primer (0,2 µM) ...
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bioRxiv - Genetics 2023Quote: ... Our method of genotyping involved PCR amplification across mutation sites using the primers described in Table 1 (purchased from Sigma Aldrich). Then ...
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bioRxiv - Genomics 2023Quote: ... Homology sequences of 600-900bp flanking the cut site of C-terminal Yy1 were amplified by PCR from iXist-ChrX genomic DNA using FastStart High Fidelity enzyme (Sigma Aldrich). DNA sequence encoding the C-terminal 3xT7-2xStrepII-FKBP12F36V tag was amplified from a plasmid gifted by R ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by neutralization with an equal volume of 40 mM Tris-HCl (pH 5.5).93 Multiplex polymerase chain reaction (PCR)-based genotyping was performed using REDTaq® polymerase per manufacturer recommendations (Millipore Sigma). Oligonucleotides were multiplexed as follows ...
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bioRxiv - Genetics 2021Quote: ... Mix-n-Stain CF 555 Antibody Labeling Kit and Mix-n-Stain CF 647 Antibody Labeling Kit (Sigma-Aldrich, MX555S100 and MX647S100) were used according to the manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... an Alexa Fluor™ 568 antibody labeling kit and a Mix-n-Stain™ CF®633 Dye antibody labeling kit (Sigma) were used according to manufacturer’s protocols.
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bioRxiv - Neuroscience 2020Quote: Primary cultures fixed and permeabilised as described for IF were used to perform PLA using a commercial kit (Duolink® PLA starter kit, Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA of control and insertion lines was extracted using a commercial kit (Spectrum™ Plant Total RNA Kit, Sigma life science) following the supplier’s instructions ...
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bioRxiv - Physiology 2021Quote: We used ELISA kits to detect the concentrations of inflammatory mediators such as TNF-α (KIT ELISA TNF-a-RAB0477-1KT, Sigma-Aldrich) and IL-6 (KIT ELISA IL-6-RAB0308-1KT ...
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bioRxiv - Developmental Biology 2022Quote: An ATP calibration curve was performed using the stabilized ATP standard stocks provided in the kit ATP Bioluminescence Assay Kit HS II (Millipore Sigma #11699709001) and following the supplied protocol ...
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bioRxiv - Molecular Biology 2023Quote: AKT:FBP1:ALDOB:PP2A-C interactions in liver tissue were detected with an in situ PLA kit (Duolink® In Situ Red Starter Kit Mouse/Rabbit, Sigma, DUO92101) (Su et al. ...
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Induction of osteogenic differentiation of MSCs by GSK3β knockdown through GSK3β siRNAs transfectionbioRxiv - Molecular Biology 2023Quote: Activity of ALPL and calcium ions concentration in cell lysates on day 7 of osteogenic differentiation were determined using reagent kits “Alkaline Phosphatase-New Liquid Form” (Vector-Best, Russia) and Calcium Colorimetric Assay Kit (Sigma-Aldrich, USA), respectively ...
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bioRxiv - Physiology 2023Quote: ... FGF21 was measured by using a commercially available enzyme-linked immunosorbent assay kit (rat/mouse FGF21 enzyme-linked immunosorbent assay kit; Millipore, Billerica, MA), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: RNA was extracted using Qiagen RNeasy Mini Kit (Maryland, USA) according to the manufacturer’s protocol and DNase-treated using the DNase I kit (Millipore Sigma, Massachusetts, USA). The quantity and quality of RNA were determined using the Nanodrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... were incubated for 45 min at 4°C with 10 µL agarose beads using a RAS-GTP pull-down assay kit (RAS Activation Assay Kit, Merck Millipore, 17-218). Supernatant was recovered after centrifugation ...
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bioRxiv - Plant Biology 2020Quote: ... using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) and DNeasy® Plant Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... the malachite green phosphate assay kit (Sigma-Aldrich, MAK307) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... and Spectrum Plant Total RNA Kit (Ref. STRN50, Sigma), respectively ...
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bioRxiv - Molecular Biology 2022Quote: The tartrate-resistant acid phosphatase (TRAP) kit (Sigma-Aldrich) was used for osteoclasts detection on paraffin sections of twelve weeks old hind paws using the TRAP kit following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... using the Glucose (GO) assay kit from Sigma Aldrich. For each assay ...
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bioRxiv - Molecular Biology 2021Quote: Phosphatase activity was measured using phosphatase assay kit (Millipore). Immunoprecipitated samples were resuspended in phosphate-free assay buffer ...
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bioRxiv - Cell Biology 2022Quote: PLA was performed using the Duolink kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: RNA was extracted by RNA miniprep kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 1 µl of L-Glutamate Dehydrogenase (Ammonia kit, Sigma), 2 µl of 150 mM NAD+ ...
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bioRxiv - Molecular Biology 2019Quote: EZ-Magna RNA-binding protein immunoprecipitation kit (Millipore, USA) was performed according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: Cholesterol concentration was estimated using a kit from Sigma/Aldrich (MAK043) ...
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bioRxiv - Molecular Biology 2019Quote: ... and GenElute™ mRNA Miniprep kit (MRN10, SIGMA-ALDRICH), respectively.
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bioRxiv - Cell Biology 2020Quote: ... 50 μl Cell Counting Kit – 8 solution (Sigma Aldrich) was added to each well and cells were incubated for 1 h in the cell culture incubator and absorptions at 450 nm was measured.
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bioRxiv - Genetics 2021Quote: ... We used Spectrum Plant Total RNA Kits (Sigma Aldrich) for RNA extraction ...
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bioRxiv - Developmental Biology 2021Quote: ... the Periodic Acid-Schiff staining kit (Millipore Sigma, 101646) procedure was followed as described by manufacturer ...
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bioRxiv - Biophysics 2021Quote: ... The Duolink In Situ Proximity Ligation kit (Sigma; DUO92007) was used as per the manufacturer’s instructions with fluorophore-conjugated secondary antibody to recognize HA (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... We used the Masson’s Trichrome Stain Kit (Sigma Aldrich) following manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2020Quote: ... live/dead cell double staining kit (04511, Sigma Aldrich), formaldehyde solution (F8775-25ML ...
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bioRxiv - Neuroscience 2021Quote: ... or the Triglyceride Quantification Colorimetric/Fluorometric Kit (Sigma, MAK266).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Lactate dehydrogenase (LDH) Activity Assay Kit (Sigma-Aldrich, MAK066), Adenosine Triphosphate (ATP ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were measured using a commercial test kit (Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... the blotted membranes were visualized with chemiluminescent kits (Millipore). Densitometric analyses were performed using ImageJ software.
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bioRxiv - Plant Biology 2020Quote: ... all chemicals and kits were obtained from Sigma-Aldrich Inc.
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bioRxiv - Microbiology 2020Quote: ... using the high capacity RNA to cDNA kit (Sigma) following manufacturer guidelines ...
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bioRxiv - Microbiology 2020Quote: ... coli using a Sigma GenElute Plasmid Kit (Sigma Aldrich). Yeast genomic DNA was isolated with the SDS/LiAc protocol [29] ...
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bioRxiv - Plant Biology 2021Quote: ... Labeling was detected using the Immobilon western kit (Millipore) or the SuperSignal West Femto Maximum sensitivity substrate (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and revealed with the NBT/BCIP Reagent Kit (Sigma). Intensities of bands were evaluated using ImageJ88 ...
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bioRxiv - Biochemistry 2021Quote: ... or the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and the Ab Buffer Kit (Sigma, GE28-9030-59) according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2019Quote: ... eluted from the gel (GelElute gel extraction kit; Sigma) and quantified via qPCR with a KAPA Library Quantification Kit for Illumina (KAPA Biosystems ...
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bioRxiv - Physiology 2020Quote: ... or RIA kit (250-tubes GL-32K, Millipore, USA) and GLP-1 ELISA kit Active (EGLP-35K ...
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bioRxiv - Plant Biology 2021Quote: ... except an On-Column DNase I Digestion kit (Sigma) was used during RNA extraction.