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Citations for Millipore Sigma :
4551 - 4600 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... A freshly prepared 2-mercaptoethylamine (2-MEA; Sigma) solution (750 mM ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 mM K3Fe(CN)6 and 2 mM acetylthiocholine iodide (Sigma-Aldrich, St. Louis, MO, USA) as reaction substrate ...
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bioRxiv - Cancer Biology 2023Quote: ... Recovered splenocytes were counted and 2-6 million cells in 100 ul sterile saline solution (Sigma) were i.p ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with 2 μm rapamycin for 6 h (Sigma-Aldrich, St Louis, MO, USA) or DMSO at the appropriate concentration as a solvent control.
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bioRxiv - Biophysics 2020Quote: ... 2 mM CaCl2 and 10 mM HEPES at pH 7.4 with 1-2 mM qBBr (Sigma-Aldrich or Toronto Research Chemicals ...
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bioRxiv - Microbiology 2022Quote: ... and 2 µg/ml L-1-Tosylamide-2-phenylethyl-chloromethyl-ketone (TPCK)-treated trypsin (Sigma-Aldrich, USA). Viral infectivity titers were measured in MDCK-II cells in 96-well plates ...
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bioRxiv - Neuroscience 2020Quote: ... protease inhibitor cocktail tablets and 2 mg/mL pepstatin A dissolved in ethanol:dimethyl sulfoxide 2:1 (Sigma). To minimize inter-blot variability ...
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bioRxiv - Microbiology 2023Quote: ... Fur was submerged in 2 mL of 2:1 (v/v) chloroform:methanol (Sigma 288306 and Sigma 322415) followed by sonication in a water bath for six minutes to dislodge lipids ...
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bioRxiv - Microbiology 2023Quote: ... Fur was submerged in 2 mL of 2:1 (v/v) chloroform:methanol (Sigma 288306 and Sigma 322415) followed by sonication in a water bath for six minutes to dislodge lipids ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets were resuspended in 200µl of LCMS-grade acetonitrile/methanol/water (2:2:1) (Sigma Aldrich) chilled to -20°C and transferred to lysing matrix Y tubes (MP Biomedicals ...
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bioRxiv - Cell Biology 2024Quote: ... 1 ml ion exchange cartridge packed with 100 mg of 2– 2(pyridyl)ethyl silica gel (Sigma) was activated with 1 ml of methanol and equilibrated with 1 ml of buffer A (methanol/H2O 1:1 ...
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bioRxiv - Cancer Biology 2023Quote: ... 3-5 million cells were fixed with ∼1% formaldehyde (Sigma-Aldrich) for 10 min and lysed ...
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bioRxiv - Cell Biology 2023Quote: ... ∼1 kb 5’ homology arm and ∼1 kb 3’ homology arm of msps genomic region were amplified from the genomic DNA (Sigma, Extract-N-Amp™ Tissue PCR Kit), mutated to be insensitive to gRNAs ...
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bioRxiv - Neuroscience 2023Quote: ... Brains of P0 pups were kept in 4% PFA in 4°C overnight and then transferred to 4°C 30% sucrose (Sigma-Aldrich, S5016) with 0.01% sodium azide (Fisher ...
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bioRxiv - Cell Biology 2023Quote: Whole fetal livers (FL) were fixed during 24h at 4°C in 4% formaldehyde and then embedded in 4% low-melting agarose (Sigma #A9539-500G). One hundred µm sections were made using a vibratome (Leica #VT1200 ...
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bioRxiv - Bioengineering 2019Quote: ... cells were permeabilized via 0.02% Tween 20 for 20 minutes and incubated overnight at 4°C with rabbit anti-SLCO1B3 (OATP1B3) primary antibody (2-μg/mL working concentration, HPA004943, Sigma-Aldrich Canada, Oakville, Ontario, Canada). Goat anti-rabbit AlexaFluor 647-conjugated secondary antibody was then applied for two hours at room temperature (1:500 dilution ...
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bioRxiv - Microbiology 2024Quote: ... Cells were cells fixed with 4% paraformaldehyde (ProSciTech, Cat. no. C004) in PBS for 30 min at RT and blocked with 2% BSA (Sigma-Aldrich, Cat. no. A8022-10G) in PBS (BSA/PBA ...
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bioRxiv - Neuroscience 2022Quote: ... For the blockade of cholinergic receptors in CA1 200nl of atropine (2 mM) and mecamylamine (2 mM) (1:1) (Sigma Aldrich) were microinjected at 1.2mm DV (IM-9B microinjector ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with anti-Sulf-1/2 primary antibody (anti-Sulf-1: rabbit polyclonal, Sigma, SAB1410410, anti-Sulf-2: rabbit monoclonal clone 2B4, Sigma, MABC584) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 50 hpf zebrafish embryos using the lysis buffer from the calpain 1/2 activity kit (InnoZyme™ Calpain 1/2 Activity Assay Kit CBA054 purchased from Merck Millipore). Protein concentration was adjusted to 2 mg/mL and calpain enzymatic activity was measured in microtubes following the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 5-fluorouracil (5-FU) : 3 µM (F6627; Sigma), cisplatin (CDDP ...
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bioRxiv - Molecular Biology 2023Quote: ... were cleaned by sonication with 3 M NaOH then Piranha solution (2:3 ratio of 30% (w/w) H2O2 to sulfuric acid) and treated with 3-glycidyloxypropyl trimethoxysilane (GOPTS) (Sigma). GOPTS was then reacted with a mixture of 9 parts α-hydroxy-ω-amino PEG 3000 to 1 part α-biotinyl-ω-amino PEG 3000 by weight (Rapp Polymere) ...
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bioRxiv - Plant Biology 2021Quote: 2-AA-labeled N-glycans prepared for HILIC-HPLC were desalted on C18 ZipTips (Millipore, Merck, Warszawa, Poland), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... were administered haloperidol (0.5 or 2 mg/kg/day; haloperidol; n=12/group: Sigma-Aldrich, Gillingham, Dorset, UK) or vehicle (β-hydroxypropylcyclodextrin ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysis was then performed by submerging slides in lysis buffer (2% N-Lauroylsarcosine sodium salt (Sigma-Aldrich), 0.5M NA2EDTA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: All samples were made to pH 6.0 in 50 mM 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Sigma), using potassium as a counterion ...
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bioRxiv - Neuroscience 2023Quote: All samples were made to pH 6.0 in 10 mM 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Sigma), using potassium as a counterion and containing 10% D2O ...
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bioRxiv - Microbiology 2022Quote: ... SK-N-BE(2) cell differentiation was induced by 10 μM retinoic acid (RA) treatment (R2625, Sigma-Aldrich) for 5 days ...
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bioRxiv - Neuroscience 2019Quote: ... before fixation with 4% paraformaldehyde/ 4% sucrose (v/v), and subsequently permeabilized (DPBS, 0.2% triton X-100 (Sigma-Aldrich #9002-93-1)) ...
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bioRxiv - Molecular Biology 2022Quote: ... cerevisiae was spiked with S. pombe total RNA (4:1 ratio S. cereviasiae : S. pombe)treated with 4-tU 500 μM final concentration (Sigma-Aldrich, 440736). The biotinylation was performed with 200ul EZ-link-HPDP-biotin (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... The organoids thus generated were re-plated at 1:4 split ratio and exposed to 400 nM 4-hydroxy tamoxifen (Sigma; Catalog #H7904) in medium to achieve the deletion of Cbl and Cblb ...
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bioRxiv - Neuroscience 2022Quote: ... 1 hemisphere of each mouse brain without cerebellum and olfactory bulbs was placed in FACS buffer (1x DPBS, 2% FCS and 2 mM EDTA) + 5 μM Actinomycin D (ActD, Sigma, Cat#A1410-5MG) for transcriptomics ...
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bioRxiv - Immunology 2020Quote: ... 50 μl of antibody exposed to hemin or hemin in PBS only were mixed with 150 μl of reaction buffer (0.15 M citrate-phosphate buffer pH 5) containing 0.9 mM of 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, Sigma-Aldrich) and 6 mM H2O2 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated overnight at 4 ° C using antibodies specific for the N-terminal amino acids 66 – 81 of the amyloid precursor protein (APP) (Millipore, Burlington MA, clone 22C11 at 1:80K), GFAP (Leica ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 g pectin,0.25 g xylan, 1 g starch, 3 g yeast extract, 1 g proteose peptone, 2 g pig gastric mucin; all from Sigma Aldrich, St. Louis, MO). The amounts of emulsifiers to add were calculated to obtain concentrations of 0.005% (m/v) ...
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bioRxiv - Cell Biology 2024Quote: ... Permeabilization buffer was removed and washed 3×2 minutes before blocking for 1 hour at room temperature in a 1% Bovine Serum Albumin (BSA) (Millipore Sigma, cat: 2910-OP) in DPBS solution ...
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bioRxiv - Biophysics 2023Quote: ... was purchased from MedChem-Express (Monmouth Junction, NJ, USA). The phospholipid 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC, see Fig. 1) was purchased from Sigma-Aldrich (Merck KGaA, Germany). Potassium bromide (KBr) ...
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bioRxiv - Neuroscience 2023Quote: ... 215 uL of either ice-cold 20mM Tris-HCl/2 mM MgCl2/0.5% Triton X-100 with protease inhibitor (Pierce #A32953) and phosphatase inhibitor cocktail 3 (Sigma #P0044; Buffer #1, Bf#1) or boiling 1%SDS/50mM NaF (Buffer #2 ...
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bioRxiv - Molecular Biology 2024Quote: ... then fully resuspended in 1 mL cold resuspension buffer supplemented with 1% each phosphatase inhibitor cocktails 2 and 3 (Sigma Cat #P5726 and P0044). To lyse the resuspended cells ...
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bioRxiv - Neuroscience 2021Quote: A solution containing 10 μM of 5-bromo-2’-deoxyuridine (BrdU; SIGMA #858811) was added to the growth medium of SK-N-SH cell monolayers and cells were incubated at 37°C for 2 hours to estimate the S-phase index ...
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bioRxiv - Biophysics 2020Quote: ... 10 mM NaN3 and 5 mM 2-deoxy-D-glucose (Sigma-Aldrich, Singapore).
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bioRxiv - Cancer Biology 2020Quote: ... 5-aza-2’-deoxycytidine was purchased from Sigma-Aldrich (Cat. A3656, Louis, USA).
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bioRxiv - Microbiology 2021Quote: ... hBMECs were subsequently incubated with 5 μM 4,6-diamidino-2-phenylindole (DAPI; Sigma) for 5 min at room temperature ...
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bioRxiv - Physiology 2021Quote: ... the DNA methyltransferase inhibitor 5-aza-2 deoxycytidine was from Sigma-Aldrich (A3656), Polyethylenglycol 300 (PEG300 ...
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bioRxiv - Molecular Biology 2020Quote: ... and the cysteine bonds reduced with 5 mM tris(2-carboxyethyl)phosphine (Sigma) (37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... and then 20 minutes with 250 μM 5-Iodo-2′-deoxyuridine (IdU, Sigma). The inhibitor and mock treatment was present during labelling ...
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bioRxiv - Molecular Biology 2020Quote: ... slides were blocked with 5% normal donkey serum and 2% BSA (Sigma Aldrich) in PBS at room temperature for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... media was changed to CM1 supplemented with 2-5 μg/mL puromycin (Sigma). On Day 3 ...
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bioRxiv - Cancer Biology 2021Quote: Cells were incubated in medium containing 2μM 5-aza-2’-deoxycytidine (azadC; Sigma) for up to 6 days ...
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bioRxiv - Immunology 2020Quote: ... or Pam2Cys + 2 mM 5-A-RU (Aubé laboratory) + 50 uM methylglyoxal (Sigma) in 200 uL on Day 0 ...