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Citations for Millipore Sigma :
4451 - 4500 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: 5’-(N-Ethylcarboxamido) adenosine (NECA) (E2387, Sigma Aldrich) adenosine receptor agonist was resuspended in DMSO at a concentration of 14mg/ml and aliquoted at -20°C following manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2019Quote: PyMT [FVB/N-Tg(MMTV-PyVT)634Mul/J] transgenic animals harboring breast tumors were treated with 4-hydroxytamoxifen (Sigma-Aldrich) (1.2 mg/kg/daily ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then solubilized overnight at 4°C in presence of 0.5% n-Dodecyl β-D-maltoside (DDM) (Sigma, D4641). Lysates were used for co-immunoprecipitation using Dynabeads M-280 Streptavidin (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: Right tibias from SMAD1/5WT and SMAD1/5iΔEC mice (n= 10 per group) were collected and fixed in 4% paraformaldehyde (PFA; Sigma Aldrich) in PBS overnight (12h ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... were identified and quantified by gas liquid chromatography (Perichrom) as their alditol acetates obtained after hydrolysis (4 N trifluoroacetic acid (TFA, Sigma), 100 °C ...
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bioRxiv - Physiology 2021Quote: ... were incubated at 4 °C overnight with 5 μg/mL anti-FLAG (Sigma-Aldrich, F1804) in 200 mM NaHCO3 buffer ...
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bioRxiv - Microbiology 2020Quote: ... 4-morpholineethanesulfonic acid hemisodium salt (5 ml, 0.5 M in MQ water; #M0164, Sigma-Aldrich) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... an uncoupling agent carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) (5 μM) (Sigma Aldrich, Missouri, USA). and antimycin-A (2 μM ...
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bioRxiv - Developmental Biology 2021Quote: ... Larvae older than 4 dpf were first soaked in 5 mg/ml epinephrine (Sigma-Adrich) for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 4-5 days with 0.5 mM EDTA in DMEM/F12 (Sigma). For introduction of TRE3G-NGN2 into the AAVS1 site ...
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bioRxiv - Bioengineering 2023Quote: ... fibroblast cells (4 ·104 cells/ml) and collagen I (5 ng/µl, Sigma-Aldrich, USA) was added to the wells of a 96-well plate ...
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bioRxiv - Immunology 2023Quote: ... and IL-5 cultured cells were pulsed (4 hr) with BrdU (10 µM) (Sigma-Aldrich) prior to flow staining with an anti-BrdU-Alexa fluor 647-conjugated antibody (BD Pharminogen ...
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bioRxiv - Systems Biology 2024Quote: ... each with 4 thoraces were homogenized in 100 µl of 5 M guanidine-HCl (Sigma) supplemented with 100 mM Tris pH 8.0 and 5 mM EDTA pH 7.8 ...
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bioRxiv - Developmental Biology 2024Quote: ... For live confocal imaging of dat:eGFP and dat:mito-RFP dopaminergic neurons larvae were mounted in 1.2% low-melting point agarose in E3 embryo media containing 160ug/ml Ethyl 3-aminobenzoate methanesulfonate (Tricaine MS-222; Sigma-Aldrich 886-86-2) Tricaine anesthetic ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was crosslinked to the membrane in 0.16 M N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (Sigma) in 0.13 M 1-methylimidazole (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... and overlaid with 0.5 μl of the matrix solution (α-cyano-4-hydroxycinnamic acid in 50% acetonitrile/0.1% TFA; 5 mg/ml, Sigma). Peptide mass maps of proteins in Figures 4f ...
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bioRxiv - Cancer Biology 2019Quote: ... containing 5% nonfat dry milk and incubated overnight at 4°C with primary antibody diluted in PBST–5% nonfat dry milk or 5% bovine serum albumin (BSA, Sigma). Membranes were subsequently washed with PBST and incubated with the appropriate IRDye 680RD and IRDye 800CW secondary antibody (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2019Quote: ... motor column explants were fixed with 1:1 mixture of 4% paraformaldehyde (Sigma) and 30% sucrose in PBS for 5 minutes followed by 4% paraformaldehyde for 5 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... tissues were fixed in 4% paraformaldehyde in 1:1 PBS and heptane (Sigma) and washed with PBST ...
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bioRxiv - Synthetic Biology 2020Quote: ... Filtered cells were mixed 1:1 with 4% molten type VII agarose (Sigma) and the cells-agarose mixture was injected into a gel apparatus and allowed to set at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide] dye solution (Sigma-Aldrich, USA) (5 mg MTT in 1 ml PBS ...
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bioRxiv - Microbiology 2019Quote: ... before being concentrated using Amicon Ultra-2 centrifugal 3 kDa filter units (Millipore Sigma). For Western blot analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with phosphatase inhibitors complex 2 and 3 (Sigma Aldrich, P5726-1ML, P6044-1ML), DTT 10 mM and protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Sigma-Aldrich) and phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich). Protein concentration was measured with BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... The coverslips were then immersed in 2% (v/v) (3-Aminopropyl)triethoxysilane (Sigma A3648) prepared in acetone for 2 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Cells were split every 2-3 days with trypsin (Sigma, Saint Louis, Missouri, USA) to maintain a subconfluent monolayer.
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bioRxiv - Cell Biology 2020Quote: ... Sigma-Aldrich) and phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich). Protein concentration was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... concentrated to 2 ml using an Amicon concentrator with a 3 kDa cutoff (Millipore), and applied into a Superdex 75 (16/600 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with phosphatase inhibitors complex 2 and 3 (Sigma Aldrich, P5726-1ML, P6044-1ML), DTT 10 mM and protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... 2× M63 buffer was made by adding 3 g KH2PO4 (Sigma-Aldrich cat. P5655), 7 g K2HPO4 (Sigma-Aldrich cat ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were subcultured every 2-3 days using trypsin (Sigma, Saint Louis, Missouri, USA) to maintain a subconfluent cell layer.
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bioRxiv - Biochemistry 2020Quote: Ebselen (2-Phenyl-1,2-benzisoselenazol-3(2H)-one) is commercially available from Sigma Aldrich.
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 1X protease inhibitor and phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich), for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 3 μM SHP1/2 phosphatase inhibitor (NSC-87877, Sigma-Aldrich) for 20 minutes prior to target particle addition ...
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bioRxiv - Genetics 2020Quote: ... Cells were washed with 3 × 100 µL of 2% bovine serum albumin (BSA, Sigma) in D-PBS+CM and blocked in that same solution for 2 hours at room temperature (RT) ...
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bioRxiv - Developmental Biology 2022Quote: Myoblasts were transfected with either (final concentrations) 10 µM 2’,3’-cGAMP (Sigma Aldrich), 2 µg/mL poly(I:C ...
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bioRxiv - Microbiology 2022Quote: ... coverslips were incubated in a 2% solution of (3-Aminopropyl)triethoxysilane (APTES, Sigma-Aldrich) in acetone with agitation for two minutes ...
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bioRxiv - Neuroscience 2022Quote: ... and 1.0% (v/v) phosphatase inhibitor cocktail #2 and #3 (Sigma-Aldrich P5726, P0044). Tissue homogenates were centrifuged at 14,000×g at 4°C for 20min and supernatants collected and stored at -80°C before initiating the protein assay.
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products were transformed into pKM208 carrying Rosseta 2 (Millipore Sigma 71400-3) strain by electroporation ...
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bioRxiv - Cell Biology 2022Quote: MCF10A cells (3×106 cells per condition) were fixed with 2% formaldehyde (Sigma, #252549) in PBS with 10% FBS for 10 min at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and Phosphatase Inhibitor Cocktails 2 and 3 (Sigma- Aldrich cat. P5726 and cat. P0044). Pellets were resuspended in lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 μL each phosphatase inhibitor cocktails 2 and 3 (Sigma Cat #P5726 and P0044)) and lysate cleared by centrifugation at 17,000g for 10 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-BUBR1-p620 (custom raised by Moravian, 1:1000) and mouse anti-α-Tubulin (clone B-5-1-2, T5168, Sigma-Aldrich, 1:10000). Secondary antibodies used were goat anti-mouse IgG HRP conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse mAbs anti-Vinculin (hVIN-1) and anti-α Tubulin (clone B-5-1-2) and rabbit polyclonal anti-Paxillin (HPA051309) were from Sigma-Aldrich and were diluted 1:400 ...
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bioRxiv - Molecular Biology 2024Quote: ... passed through a 0.45 µm filter and used to infect either HEK293 cells in a dilution of 1:5 or RAW macrophages in a dilution of 1:2 with 8 µg/ml polybrene (Sigma-Aldrich, TR1003G). Transduced cells were selected by either puromycin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with avertin (2, 2, 2-tribromoethanol, Sigma Aldrich, St. Louis, MO) or isoflurane for stereotaxic surgery (Model 1900 Stereotaxic Alignment System ...
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bioRxiv - Immunology 2023Quote: ... After general anesthesia with i.p.-administered Avertin (2, 2, 2-tribromoethanol, Sigma-Aldrich #T48402) the blood was collected with heparinized capillaries (Fisher Scientific™ #22-260950 ...
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bioRxiv - Neuroscience 2021Quote: ... 2.2 mW.mm-2 for 5-10 ms) in control and in the presence of 1 µM strychnine (Sigma-Aldrich), 20 µM DNQX and 10 µM gabazine (BioTechne ...
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bioRxiv - Cell Biology 2021Quote: ... 12-24hr embryo lysates were collected by homogenizing dechorionated embryos in a Dounce homogenizer with 100 μl of lysis buffer (60mM Tris pH7.5, 80mM NaCl, 6mM EDTA pH8.0, 2% Triton X-100, 5mM 1-Naphthyl potassium phosphate, 2mM PMSF, 1X Sigma Phosphatase Inhibitor II ...
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bioRxiv - Microbiology 2021Quote: ... livers were finely minced with a sterile razor blade and digested overnight at 37°C in 40 mL 1×PBS containing 2% penicillin/streptomycin/amphotericin B (1×PBS −2% PSF) and 5 mL 0.5% clostridial collagenase solution (0.025 g of collagenase (Sigma, C0130) in 5 mL of dH20 for immediate use) ...