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Citations for Millipore Sigma :
4451 - 4463 of 4463 citations for Recombinant Human IL12B Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... was performed in a representative subset of our participants using an adapted version of the MILLIPLEX® Human High Sensitivity T Cell Magnetic Bead Panel (Millipore Sigma, Burlington, MA, USA). The MAGPIX® Luminex analyzer (Luminex Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human mesenchymal stem cells (MSCs) were cultured on gelatin coated flasks for 2-3 days in Alpha-MEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... After washing, IgE antibodies were detected using biotinylated goat anti-human IgE (Vector, Burlingame, CA, USA) and streptavidin peroxidase (Sigma-Aldrich, St. Louis, MO, USA). The wells were then washed again with PBST buffer and incubated with 100 ul 3,3’,5,5’- Tetramethylbenzidine (Kirkegaard and Perry Laboratories ...
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bioRxiv - Immunology 2024Quote: ... and IFN-γ were measured in the full Generation R cohort using the Human High Sensitivity T-Helper Cells Custom 5-plex assay from Millipore (Millipore, St. Charles, MO, USA) on the Luminex™ 100 system (Luminex ...
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bioRxiv - Microbiology 2024Quote: ... blood mononuclear cells were isolated by density gradient centrifugation from buffy coats of human healthy donors (Ficoll-Paque Premium, Sigma Aldrich, St. Louis, MI, USA). Monocytes were purified from peripheral blood mononuclear cells by positive selection with magnetic CD14 MicroBeads (Miltenyi Biotech ...
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bioRxiv - Bioengineering 2020Quote: ... co-staining was performed using rabbit anti-perilipin A antibody as an adipocyte marker in combination with a mouse anti-human mitochondria (hMito) antibody conjugated with Cy3 (EMD Millipore, Burlington, USA; 1:100 dilution). hMito was used as an alternative to Ku80 based on the availability of antibodies compatible for co-staining ...
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bioRxiv - Cancer Biology 2021Quote: ... ERK5 was silenced with human or mouse shRNAs purchased as pre-cloned in the pLKO.1-puro plasmid (MISSION® shRNA, Sigma-Aldrich, St Louis, MO, USA). Control shRNAs in the same plasmid were also purchased (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... transferrin from human serum, (Tf), cytosine (Cyt), trifluoroacetic acid (TFA, ≥99%), and formic acid (FA, analytical grade >98%) were purchased from Sigma Aldrich (St. Louis, Missouri, United States). Methanol (MeOH) ...
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bioRxiv - Immunology 2021Quote: ... secondary antibody (goat anti-human γ-chain-specific whole IgG, α-chain-specific IgA, or μ-chain-specific IgM alkaline phosphatase conjugate, Sigma Aldrich, MO, US) was diluted 1:1000 and 50μl was added per well for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... the autoclaved peg lid was incubated in a flat-bottom 96-well plate with 200 µl of 100 µg/ml fibrinogen from human plasma (product number F3879, Sigma-Aldrich, in 0.9% NaCl or PBS) at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: HEK293T cells were transfected as described above with empty or human SLFN14 expression plasmids and a plasmid encoding Hluc in the presence of RNA Pol III inhibitor (Sigma ML-60218, 40 and 20 uM) or tofacitinib (Sigma PZ0017 ...
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bioRxiv - Cancer Biology 2023Quote: ... and HPCD-3BP were incubated at various concentrations (12.5 μM, 25 μM, 50 μM, 100 μM, and 200 μM) with 90 μL of human serum (Sigma, Cat No. H3667; St. Louis, MO, USA) at 37 °C for up to 8 h ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in matched BAL fluid and plasma collected from patients were measured using the multiplexed human cytokine/chemokine magnetic bead kits from Millipore (HCYTMAG-60K-PX41 and HCYP2MAG-62K) according to manufacturer’s protocol (HCYTOMAG-60K Rev ...