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Citations for Millipore Sigma :
4451 - 4500 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The FLAG-tagged PCRK2 and PBL19 proteins were immunoprecipitated using 15 μl M2 beads (Sigma; Cat. #A2220). Biotinylation was detected with Streptavidin-HRP (Abcam Cat ...
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bioRxiv - Plant Biology 2021Quote: ... The FLAG-tagged NRG1C and SAG101 proteins were immunoprecipitated using 20 µl M2 beads (Sigma; Cat. #A2220) for co-immunoprecipitation and biotinylation was detected by Streptavidin-HRP (Abcam Cat ...
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bioRxiv - Neuroscience 2020Quote: ... Antibody-bound chromatin complex was precipitated with salmon sperm DNA/Protein A agarose (EMD Millipore, Burlington, MA), then washed sequentially with PIRA (150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were blotted as previously described and detected using Anti-polyHistidine-Peroxidase monoclonal antibody (Sigma-Aldrich # A7058) and signals were revealed using the Metal Enhanced DAB Substrate Kit ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were separated by SDS-PAGE and probed with antibodies against zDHHC5 (1:1,000; Sigma Prestige, HPA014670), HA (1:1,000 ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 mg of protein was mixed with 20 μL of anti-FLAG M2 magnetic bead slurry (Sigma) in a 1 mL volume ...
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bioRxiv - Microbiology 2021Quote: ... protein extracts were incubated with 30 μL of Anti-Flag® M2 Affinity Gel beads (Sigma-Aldrich) at 4°C for 4 h ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Neuroscience 2020Quote: ... 20 μg of proteins were separated by 10% SDS-PAGE and transferred to Immobilon-FL membranes (Millipore). For dot-blot assays ...
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bioRxiv - Microbiology 2021Quote: ... Two microlitres of the supernatant was used for protein quantification using the Direct Detect system (Merck Millipore). The acquired concentrations of intracellular c-di-GMP were normalized to the total protein content.
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bioRxiv - Microbiology 2021Quote: ... Protein samples were separated by SDS–PAGE (4-20%) and blotted onto polyvinylidene fluoride membranes (Merck Millipore). Membranes were blocked for 1 h with Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then detected with monoclonal mouse anti-FLAG M2 (1:1000; Sigma-Aldrich, St. Louis, MO) and polyclonal rabbit anti-6xHis (1:2,000 ...
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bioRxiv - Microbiology 2021Quote: ATPase activity of Wt and mutant HelR proteins was determined using the colorimetric ATPase assay kit (Sigma). The ATP hydrolysis reaction was performed by incubating 0.3 µM HelR with 1 mM ATP (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... Prior to immunization and subsequent boosts protein was concentrated with 10,000 NMWL centrifugal filter units (EMD Millipore).
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bioRxiv - Microbiology 2020Quote: ... HA3-tagged proteins were detected using a monoclonal rat α-HA3 (1:1000; clone 3F10; Sigma-Aldrich). Actin ...
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bioRxiv - Neuroscience 2021Quote: ... The protein content was quantified according to the Coomassie blue method using bovine serum albumin (Sigma-Aldrich) as a standard [36] ...
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bioRxiv - Cancer Biology 2020Quote: ... and the eluted protein sample was processed with an Amicon Ultra 0.5 3k centrifugal filter device (Millipore) for concentration and buffer exchange to 50 mM Tris pH 8.5 ...
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bioRxiv - Biophysics 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 100-kDa cut off, Millipore Sigma) for gel filtration (GF) ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were transferred on an Immobilon™-P Membran (PVDF, 0.45 µm, Art. No. IPVH00010, Merck Millipore) for 16 h at 15 V using a Mini Trans-Blot® Cell (Art ...
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bioRxiv - Cell Biology 2022Quote: ... Equivalent amounts of protein were resolved by SDS-PAGE and transferred to polyvinylidene fluoride microporous membrane (Millipore), blocked with 1.5% BSA in H20 containing 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were separated in SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) filter membranes (Millipore, USA) for immune-hybridization ...
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bioRxiv - Cancer Biology 2022Quote: The RIP assay was performed using a Magna RIPTM RNA Binding Protein Immunoprecipitation Kit (17-700; Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded protein was concentrated with Amicon Ultra-15 Centrifugal Filter Units (100 kDa NMWL, Merck Millipore) and remaining urea removed with a PD10 desalting column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... Bound proteins were eluted using IP buffer containing 0.25 mg/mL of 3x FLAG peptides (Sigma-Aldrich) at 25 °C for 5 min ...
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bioRxiv - Plant Biology 2022Quote: ... proteins and DNA fragments were then immuno-precipitated using antibodies against anti-FLAG (SIGMA, catalog no. M2), H3Ac (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant MejAgo protein expression and Ni-affinity chromatography purification procedures were performed according to user manual(Novagen). In perticular ...
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bioRxiv - Microbiology 2022Quote: Total protein was determined by Coomassie blue method using Bradford dye (Sigma-Aldrich, St. Louis, MO, USA) and absorbance was measured at 595 nm ...
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bioRxiv - Microbiology 2022Quote: ... and the recombinant protein expression was induced with 0.5 mM isopropyl-D-1-thiogalactopyranoside (IPTG; Sigma-Aldrich). The bacteria were incubated at 18°C for additional 18 hours ...
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bioRxiv - Genetics 2022Quote: ... Protein samples were prepared in 4X NuPAGE LDS Sample Buffer with 0.5% b-mercaptoethanol (Sigma, catalog# M6250) for all proteins except for PC1 and PC2 and their loading control beta-actin ...
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bioRxiv - Biochemistry 2022Quote: ... 150mM NaCl and bound protein was eluted with the same buffer containing 2.5 mM desthiobiotin (Sigma-Aldrich). Proteins were spun at 100,000x g to remove aggregates before use in assays ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were eluted by the addition of elution buffer containing 3 x Flag peptide (Sigma, cat. #F4799) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to 8.0 mg/ml using 100k molecular weight cutoff Amicon columns (UFC510024, Millipore) and further purified by gel filtration chromatography (Superose 6 increase 3.2/300 GL ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was filtered through a 0.2 µm low-protein binding syringe filter (Millex Durapore; EMD Millipore) and applied to glutathione Sepharose 4b resin (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... and HA-tagged full length CASK proteins were incubated with 1μg anti-Flag M2 antibody (Sigma, F3165) at 4°C over night ...
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bioRxiv - Cell Biology 2022Quote: Flag-tagged proteins were captured from 1 mg total cell lysate using anti-Flag affinity matrix (Sigma) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then transferred to a Amersham™ Protran® Western-Blotting-Membrane (#GE10600044, Sigma-Aldrich) at 120 V for 1 h ...
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bioRxiv - Plant Biology 2021Quote: ... HA-tagged proteins were detected with an Anti-HA-Peroxidase antibody (Sigma-Aldrich, St. Louis, USA, 12013819001). For the RACB ubiquitination screening ...
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bioRxiv - Biochemistry 2021Quote: Denatured proteins were separated by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore IPFL00010). Membranes were incubated in a casein-based blocking buffer (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl of the supernatant were used for protein quantification using the Direct Detect system (Merck Millipore).
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bioRxiv - Microbiology 2021Quote: ... GGG-azide labelled proteins were concentrated to 25 μM on a 30 kDa Amicon Tube (Merck Millipore) in Tris/NaCl buffer and next labelled with 100 μM DBCO-Cy5 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The protein samples were separated by SDS-PAGE and transferred to PVDF membranes (Merck Millipore, Burlington, MA) by electroblotting ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were prepared and TRF2-containing complexes were immunoprecipitated using anti-FLAG M2 affinity gel (Sigma). Following the IP ...
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bioRxiv - Biophysics 2020Quote: The RBD and spike proteins were chemically biotinylated using the EZ-Link NHS-PEG4-Biotin kit (Sigma). For the RBD ...
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bioRxiv - Neuroscience 2020Quote: ... The protein solution was reconstituted into mesophase using molten monoolein spiked with 5% (w/w) cholesterol (Sigma) in 2:3 ratio of protein solution ...
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bioRxiv - Microbiology 2021Quote: ... Equivalent protein amounts were resolved by SDS-PAGE and subsequently transferred to Immobilon PVDF-FL membranes (Millipore). Blots were probed using mouse anti-Flag M2 (1:3000 ...
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bioRxiv - Bioengineering 2019Quote: RanBP9 was immunoprecipitated from total protein extracts using monoclonal αHA-Agarose antibody (Sigma cat. nr. A2095-1ML). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... The purified protein was further concentrated on Amicon Ultra Centrifugal Filters with 50 KDa cutoff (Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... After that sections were incubated with rabbit polyclonal anti-glial fibrillary acidic protein (GFAP, Cat. No.G9269, Sigma–Aldrich Co. ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 10 kDa cut off, Millipore Sigma) for gel filtration ...