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Citations for Millipore Sigma :
4301 - 4350 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of protein were incubated with 20 µL of FLAG M2 magnetic beads (Sigma, M8823), or Pierce™ HA magnetic beads (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... GST and GST-LegK2 proteins were labelled with an anti-GST antibody from rabbit (A7340; Sigma) and detected with an anti-rabbit secondary antibody from goat conjugated with the fluorochrome Alexa Fluor 488 (A11034 ...
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bioRxiv - Developmental Biology 2023Quote: ... but those for detection of phosphorylated proteins were blocked overnight in 5% gel fish (Sigma, #G7765). Blots were probed with antibodies listed in Suppl ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Merck Millipore, cat #IPFL00010). After blocking with Intercept TBS Blocking Buffer (LI-COR ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mg of total proteins was incubated with 30 μL M2 FLAG magnetic beads (Sigma-Aldrich) for ATM and 2 mg DNA-PKcs antibody and 30 μL Protein A/G beads for DNA-PKcs ...
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bioRxiv - Neuroscience 2023Quote: ... and bands were normalized to total protein and the housekeeping gene Gapdh (1:10,000, Millipore, NP_002037).).
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bioRxiv - Microbiology 2023Quote: ... protein lysates were added to 50 ul of prewashed ANTI-FLAG M2 Affinity Gel beads (Sigma) and incubated at 4°C overnight with gentle rotation ...
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bioRxiv - Genetics 2023Quote: ... Proteins tagged with 6His10FLAG were detected by immunoblot-ting using an anti-FLAG mouse antibody (Sigma ANTI-FLAG M2 antibody F3165 ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... Protein substrates were added to degradation reactions at the following concentrations: 40 mM β-casein (Sigma), 15 µM Arc-st11-ssrA ...
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bioRxiv - Microbiology 2023Quote: Total protein content in all cell extracts was determined using the QuantiPro BCA Assay Kit (Sigma).
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bioRxiv - Bioengineering 2023Quote: ... fibrinogen stock (4 mg of clottable protein/mL of BMEM, bovine plasma, Type I-S, Sigma) was filter sterilized and mixed with 0.5 U/mL thrombin ...
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bioRxiv - Pathology 2023Quote: ... Proteins were detected with the following primary antibodies: mouse monoclonal anti-CnB (Sigma, 0581, 1:1000), rabbit polyclonal anti-CnA (Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... DNA was then added to MagnaChip magnetic protein A/G beads (16-663, Millipore, Burlington, USA) overnight that were incubated with either 5-hmC ...
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bioRxiv - Molecular Biology 2023Quote: ... Ifi205 proteins corresponding fractions were combined and purified by ANTI-FLAG M2 Affinity Gel (Sigma, A2220). A small portion of the final proteins were run through the Superdex 200 columns (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The purification of monoclonal antibodies was performed by affinity chromatography using Hitrap Protein G Column (Sigma) starting from 150 ml of hybridoma cell supernatant ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged proteins were eluted from the beads using 150 ng/ml of 3xFLAG peptide (Sigma) in 50–100 ul of elution buffer (20 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Developmental Biology 2023Quote: ... detection kit and LI-COR Odyssey platform for western blot analyses of phosphorylated proteins (Millipore Sigma). Fg-Stk36WT and Fg-Stk36AA were immunopurified from HEK293 cells transfected with the Fg-Stk36WT or Fg-Stk36AA construct.
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bioRxiv - Microbiology 2023Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd.), and the progeny virus stock was aliquoted and stored at −20°C until use.
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bioRxiv - Microbiology 2023Quote: ... the pellets were washed first with 1× BugBuster® (10× Protein Extraction Reagent Novagen® Merck), then twice with water and once with 50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Immunology 2023Quote: ... The protein antibody used for western blotting was mouse or rabbit anti-6xHis antibodies (Millipore-Sigma). Secondary antibodies were IRDye 680RD goat anti-mouse and/or IRDye 800CW goat anti-rabbit (Li-Cor) ...
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bioRxiv - Immunology 2023Quote: ... The protein antibody used for western blotting was mouse or rabbit anti-6xHis antibodies (Millipore-Sigma). Secondary antibodies were IRDye 680RD goat anti-mouse and/or IRDye 800CW goat anti-rabbit (Li-Cor) ...
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bioRxiv - Immunology 2023Quote: ... Protein bands were visualized by treating the membrane with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Darmstadt ...
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bioRxiv - Cell Biology 2023Quote: ... 35 μg of proteins were supplemented with 0.004% Bromophenol blue and 1% β-mercaptoethanol (Sigma-Aldrich), incubated at 95 °C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Secreted JAGGED1-Fc was recovered from the culture media on Protein A agarose (Millipore, 16-125) and eluted with 100 mM glycine ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-v5 antibody and 40 µl of Protein G agarose beads slurry (Millipore, 16-266) in the presence of RNase A (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 10k-cutoff filter (Millipore) and loaded onto a HiLoad Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 3k-cutoff filter (Millipore) and loaded onto a HiLoad Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... followed by the addition of 100 ng of active SLO protein reduced by TCEP (Sigma Aldrich) and 5 ug of corresponding IgG and/or IgG fragments ...
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bioRxiv - Bioengineering 2023Quote: ... was prepared at a concentration of 5 mg/mL by dissolving fibrinogen protein (crystals, Sigma-Aldrich) in sterile phosphate-buffered saline (PBS ...
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bioRxiv - Biophysics 2023Quote: ... Protein in the supernatant was quantified on a plate reader using the Bradford assay (Sigma Aldrich). All samples were prepared in loading dye (NU-PAGE dye + 5mM BME ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by standard SDS-PAGE and transferred to a PVDF (polyvinylidine difluoride) membrane (Millipore). The membrane was blocked with TBS-T (20 mM Tris-HCl ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: Cells were lysed in cold lysis buffer (CytoBuster Protein Extraction Reagent, Novagen Inc., Madison, WI, USA) in the presence of a protease inhibitor cocktail (Complete Mini ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: We extracted total protein from cells using RIPA lysis and extraction buffer (Millipore Sigma. Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... HLA-G2 proteins in PBS were concentrated by Amicon Ultra-15 (NMWL 10,000 Da) (Merck Millipore), and the flowthrough solution from protein concentration was used as a negative control (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Fractions containing recombinant protein were pooled and concentrated on a 30K Amicon Ultra Centrifugal filter (Millipore). Glycerol at 10% final was added to the sample and protein concentration was estimated using Bradford detergent compatible assay (Pierce) ...
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bioRxiv - Immunology 2024Quote: ... Recombinant lyophilized proteins were resuspended using 0.1% (w/v) human serum albumin (HSA) (A9731, Sigma-Aldrich) in sterile water ...
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bioRxiv - Biophysics 2024Quote: ... The denatured protein was then reduced with 5 μL of 500 mM dithiothreitol (DTT; Sigma-Aldrich) at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... and equilibrated before FLAG tagged proteins were immunoprecipitated using anti-Flag M2 agarose beads (Sigma #A2220).
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred on a PolyVinyliDene Fluoride (PVDF) membrane (Immobilon-P, Merck Millipore, Burlington, USA) using liquid transfer with 20 % methanol at 100V ...
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bioRxiv - Neuroscience 2024Quote: ... Staining was performed against glial fibrillary acidic protein (mouse anti GFAP−Cy3™, Sigma-Aldrich, C9205), Secondary antibody was anti-mouse Alexa Fluor 488 (Thermo-Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... and then the proteins in the gel were transferred onto Immobilon-FL polyvinylidene difluoride membranes (Millipore). The membranes were blocked with BlockPRO Protein-free Blocking Buffer (Visual Protein ...
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bioRxiv - Molecular Biology 2024Quote: ... the instrument was calibrated using a protein standard containing 10 nM β-amylase (Sigma Aldrich A8781) and 3 nM Thyroglobulin (Sigma-Aldrich 609310) ...
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bioRxiv - Biochemistry 2024Quote: ... and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma 16758) at an OD of 0.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were prepared with 25 μg of homogenate and 400 ng of yeast enolase protein (Sigma) as an internal protein control ...
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bioRxiv - Biochemistry 2024Quote: Optional concentration of protein samples using Amicon® Ultra centrifugal filters (Merck Millipore Ltd., Carrigtwohill, IRL) was performed prior to buffer exchange to reduce sample volume ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were transferred to nitrocellulose membranes (Amersham) upon which M2 Anti-FLAG mouse antibodies (Sigma) were used to visualize 3X-FLAG fusion peptides.
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bioRxiv - Cancer Biology 2024Quote: ... The separated proteins were then electro-transferred on 0.45-m polyvinylidene difluoride membranes (Millipore, Billerica, USA). The membranes were subsequently blocked using 5% skim milk for two hours at room temperature after being washed three times with TBST ...
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bioRxiv - Biophysics 2024Quote: Three different buffers were used in DHFR protein purification: Lysis buffer [100 mM bicine (Sigma-Aldrich), 5 mM imidazole (Millipore-Sigma OmniPur®) ...
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bioRxiv - Bioengineering 2024Quote: ... The final protein was then concentrated by using Amicon Ultra 30 kDa MWCO filters (Millipore Sigma).
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bioRxiv - Biophysics 2024Quote: ... The eluted protein was concentrated with a 15 ml 10000 MWCO spin filter (Merck Millipore Ltd.). The sample was centrifuged at 20.000 g for 15 min to remove precipitated protein prior to being loaded onto a HiLoad 16/600 Superdex 200 size exclusion chromatography (SEC ...