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Citations for Millipore Sigma :
401 - 450 of 507 citations for docosyl dihydrogen citrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Slides were deparaffinized and subjected to hydrolytic autoclaving at 121 °C for 10 min in citrate buffer (Sigma, C9999). Following blocking with 10% normal goat serum (Vector laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... the purified THCAS solution was buffer-exchanged into ≈ 40 µL of 100 mM sodium citrate (pH 5.0) with a 10 kDA cut-off Amicon® Ultra-0.5 Centrifugal Filter Unit (Millipore). Enzyme was kept on ice and used fresh for enzyme assay ...
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bioRxiv - Microbiology 2023Quote: Sections were deparaffinized in xylene and rehydrated in a descending ethanol series prior to heat-induced antigen unmasking in Citrate Buffer pH 6.0 (Sigma). After blocking of nonspecific binding in PBS-T containing 10% goat serum and 1% BSA ...
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bioRxiv - Microbiology 2023Quote: ... The dried powders were suspended in 100 ml of 90°C pre-heated distilled water containing 0.2 g sodium citrate (Sigma) and 350 μl 10 M NaOH ...
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bioRxiv - Developmental Biology 2023Quote: ... 5μm E14.5 paraffine sections underwent deparaffinization and microwave antigen retrieval in pH 6.0 sodium citrate with 0.05% Tween-20 solution (Sigma-Aldrich), followed by gradual chilling ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue cross-sections were deparaffinized and rehydrated and subjected to antigen retrieval using citrate buffer (0.0126 M citric acid, Cat. No. C-0759, Sigma; 0.0874 M sodium citrate ...
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bioRxiv - Systems Biology 2024Quote: ... Pre-permeabilization reagent removal was followed by a 180μl wash in 0.1X Saline Sodium Citrate (SSC, Sigma-Aldrich, USA) at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval for tissues from synaptophysin staining was facilitated by incubating in citrate buffer at 10 mM with 0.05% Tween-20 (pH 6.0, Sigma-Aldrich Cat ...
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bioRxiv - Bioengineering 2022Quote: ... 1 g L−1 (NH4)2-H-citrate and 1 mL antifoam (Antifoam 204, Sigma-Aldrich, St. Louis, MO, USA). The medium was supplemented with with 2 mL L−1 trace elements solution and (1 M ...
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bioRxiv - Physiology 2020Quote: ... followed by antigen retrieval in citrate buffer (10 mM, pH=6.0) for 40 min in 90□ and blocking in 2% BSA (A2153, Sigma Aldrich) with 0,3 M glycine (G8898 ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 µl of cell suspension were incubated with 200 µl of 50 mM sodium citrate buffer containing 4 µg/mL propidium iodide (Sigma) for at least 2 hours in darkness ...
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bioRxiv - Physiology 2020Quote: ... The sections were then post-stained with uranyl acetate and lead citrate prior to imaging with a scanning electron microscope (Zeiss SIGMA) using a back-scattered electron detector and a pixel size of 4nm.
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bioRxiv - Cell Biology 2021Quote: ... Sections were contrasted with 0,05% Oolong tea extract (OTE) for 30 minutes and 0.08 M lead citrate (Sigma-Aldrich, 15326) for 8 minutes ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... followed by washing with 50 % methanol and 30-sec staining in Reynold’s lead citrate (lead nitrate from BDH Biochemicals Ltd., Trisodiumcitrate from Sigma-Aldrich). Grids were rinsed thoroughly with deionized water and dried before imaging ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and antigen retrieval was performed in 37 °C trypsin (pH 7.8) for 1 h or 10 mM sodium citrate buffer (pH 6) (Sigma-Aldrich) at 95 °C for 10 min ...
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bioRxiv - Bioengineering 2020Quote: ... Terminal blood collection was conducted via retro-orbital bleeding from animals under anesthesia with 3.2% wt/vol sodium citrate (Cat. No. S4641; Sigma Aldrich) anticoagulant solution ...
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bioRxiv - Molecular Biology 2022Quote: ... 16806-25] in 10 mM Tris-HCl [pH 8.0], 2× saline sodium citrate [SSC], glucose oxidase [37 µg/mL, Sigma-Aldrich, G2133-10KU] ...
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bioRxiv - Genetics 2022Quote: ... Fixed cells were pelleted and incubated overnight at 50 °C in 500 µL Sodium Citrate (50 mM) with 0.7 mL RNase A (30 mg/ml; Sigma-Aldrich). Cell suspensions were treated with proteinase K (20 mg/ml ...
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bioRxiv - Immunology 2022Quote: ... and washed four times in citrate-PBS supplemented with 0.01% bovine serum albumin (BSA, 10735086001, Sigma-Aldrich, Zwijndrecht, The Netherlands). PBMCs were frozen at 105 cells/ml in freezing medium containing RPMI-1640 (RPMI ...
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bioRxiv - Neuroscience 2022Quote: ... thin sections (70 nm) from resin blocks were cut and stained with Uranyless solution (ProSciTech) for 7 minutes followed by lead citrate (Sigma) for 7 minutes ...
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bioRxiv - Systems Biology 2022Quote: ... of 5,5’dithiobis-2-nitrobenzoic acid conversion to 2-nitro-5-thiobenzoic acid in the presence of Coenzyme A thiol generated during citrate production (CS0720, Sigma) as previously described.62
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bioRxiv - Developmental Biology 2021Quote: ... sections were subject to 0.1M citrate antigen retrieval at 95°C for 10 mins before the primary antibody for type-II collagen (Millipore, MAB8887), ACAN [30] or lubricin (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... tissues were incubated until dissolved at 37°C in 495 μL extraction buffer [(4 M urea, 0.2 M NaCl, 1 mM trisodium citrate, 1% SDS (Sigma-Aldrich)] supplemented with 5 μL Proteinase K (20 mg mL−1 ...
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bioRxiv - Cell Biology 2021Quote: ... measuring its ability to prevent heat denaturation of two protein substrates: pig citrate synthase (CS) (0.2 μM; MW 51 kDa; Sigma Aldrich), and catalase purified from bovine liver (2 μM ...
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bioRxiv - Immunology 2021Quote: Blood was collected from the retro-orbital sinus into 1:10 volume of citrate phosphate dextrose (CPD; Sigma-Aldrich, #C7165) and platelet-rich plasma (PRP ...
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bioRxiv - Genomics 2022Quote: ... 75μl of a mix of the 16S probe (5’-GGATTAGATACCCBDGTAGTCGAGATNBAAAAAAAAAAAAAAAAAAAA-3’, IDT, USA) at 0.6μM concentration with 4X Saline Sodium Citrate (SSC) (SSC, Sigma-Aldrich, USA) and 0.1% SDS (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 17 mM sodium citrate, 5 mM EDTA, 0.5 mM DTT, and protease and phosphatase inhibitor cocktail [1:100]; Sigma-Aldrich) for 10 min ...
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bioRxiv - Physiology 2023Quote: ... of 5,5’dithiobis-2-nitrobenzoic acid conversion to 2-nitro-5-thiobenzoic acid in the presence of Coenzyme A thiol generated during citrate production (CS0720, Sigma) as previously described (Crouch et al. ...
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bioRxiv - Physiology 2022Quote: ... Mitochondrial content was measured using approximately 5-10 mg of tissue with a colorimetric citrate synthase activity kit (Sigma MAK193).
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bioRxiv - Microbiology 2022Quote: ... putative probiotic candidates were screened on Vibrio-selective Thiosulfate Citrate Bile Salt Sucrose (TCBS; Becton Dickinson or Sigma-Aldrich, USA) agar to identify and exclude Vibrio spp ...
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bioRxiv - Bioengineering 2022Quote: ... and antigen retrieval at 98°C for 15 minutes (KOS microwave tissue processor, Milestone) in citrate buffer pH6.0 (Sigma, C999). Prior to staining ...
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bioRxiv - Cell Biology 2024Quote: ... in which the 2x SSC buffer (300 mM NaCl, 30 mM sodium citrate, pH = 7.0) was diluted from 20x SSC (Sigma S6639) using UltraPure DNase/RNase-Free Distilled Water (Thermo 10977015) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were further dissociated via gentle up-and-down pipetting and the reaction was stopped with the addition of 400 μl of 2 mg/ml ovalbumin-enriched cold FACS buffer (2mg/mL glucose, 0.1% BSA, 1:50 Citrate Phosphate Dextrose from Sigma (C7165), 10U/mL DNase I ...
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bioRxiv - Neuroscience 2024Quote: ... Spinal cord sections on slides were first put in a plastic or glass Coplin jar with 1x citrate buffer solution at pH 6.0 (Sigma C9999), then boiled in a pressure cooker for 2 min ...
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bioRxiv - Neuroscience 2023Quote: ... rehydrated sections were incubated in heated 10 mM sodium citrate buffer (in 1X PBS, pH 6.0; Sigma, Cat#C8532-500G) for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed cells were pelleted and washed twice with PBS and resuspended in 300 μL hypotonic buffer (1 g/L sodium citrate buffer, 0.1% Triton-X 100) supplemented with 40 μg/mL propidium iodide (Sigma-Aldrich) and 100 μg/mL of RNase A (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... Cells attached to each array were washed with 100 μl of 0.1 x saline-sodium citrate buffer (SCC) (Sigma-Aldrich) diluted with nuclease free water ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were subsequently rinsed with cold running tap water for 5 min followed by antigen retrieval using citrate buffer at pH 6.0 (Sigma Aldrich). Afterwards ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue cross-sections were deparaffinized and rehydrated and subjected to antigen retrieval using citrate buffer (0.0126 M citric acid, Cat. No. C-0759, Sigma; 0.0874 M sodium citrate, Cat. No. S-4641, Sigma; pH 6.0) for a total of 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... spinal cord sections underwent antigen retrieval at 85 °C for 10 min with 1X citrate buffer pH 6.0 (Sigma-Aldrich). Tissue sections were washed with PBS and permeabilised with 1% Triton-X-100 in PBS for 30 min at RT ...
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bioRxiv - Immunology 2024Quote: ... The migrated neutrophils in the apical compartment were lysed with 0.5% Triton X-100 and neutrophil MPO activity was quantified using a colorimetric assay using citrate buffered 2,2’-azino-bis(3-ethylbenxothiazoline-6-sulfonic acid) diammonium salt (ABTS) solution (Sigma-Aldrich). After 10 min of incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... Crystal violet-stained cells were solubilized with 100 µL of a solution containing 100 nM sodium citrate (#W302600, Sigma-Aldrich) and 50% ethanol and quantified using a plate reader at 550 nm excitation ...
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bioRxiv - Physiology 2024Quote: ... slides were incubated at room temperature for 6 hours in staining solution containing 15 mM citrate buffer pH 5.3 (Sigma Aldrich), 2.8 mM CuSO (Sigma Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... the nanoparticles were washed by centrifugation at 20,000 g once and then re-dispersed in 2 mL of a 6 mM sodium citrate solution (Sigma-Aldrich). Next ...
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bioRxiv - Cell Biology 2021Quote: ... AURKA antigen retrieval was performed by incubating cells previously fixed in 4% PFA/0.2% Glutaraldehyde in a 10 mM sodium citrate tribasic dihydrate solution (Sigma-Aldrich, C8532), pH 6 at 98°C on a hot plate for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos are rinsed in HB-buffer (HB+ without torula RNA and heparin) supplemented with saline-sodium citrate buffer (SSC, Sigma, S6639) and then transferred gradually to PBT ...
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bioRxiv - Immunology 2022Quote: ... Sections were washed 3 times in PBS followed by antigen retrieval for 20 minutes with steam using 1X Citrate buffer (Millipore, 21545), pH=6.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Ultrathin sections were stained with 4% uranyl acetate for 12 min in dark at room temperature and citrate mixture (SIGMA-ALDRICH) for 2 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Slides were subsequently boiled with antigen retrieval buffer (Tris-EDTA, pH9, made in house or citrate buffer, pH6, Sigma C999-1000mL) and blocked for one hour with PBS containing 1% bovine serum albumin (BSA) ...
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bioRxiv - Immunology 2019Quote: Blood derived from healthy human volunteers was collected into citrate tubes (Becton Dickinson) and layered into Ficoll-Paque Plus (Sigma-Aldrich). Samples were centrifuged at 1440 rpm in a swinging bucket centrifuge for 20 min at 23°C without braking ...