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Citations for Millipore Sigma :
451 - 500 of 507 citations for docosyl dihydrogen citrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... glycans were co-crystallized with a solution of 50% acetonitrile/50% 20 mM ammonium citrate containing 134 mM of 2’,4’,6’-Trihydroxyacetophenone monohydrate (Sigma-Aldrich). MALDI-ToF MS analysis of the N-glycans was performed in the reflector positive and negative ion mode using a 4800 Plus MALDI ToF/ToF Analyzer (AB Sciex ...
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bioRxiv - Systems Biology 2020Quote: ... beads were recovered from the emulsion by washing with 2 × 30 mL 6 × saline sodium citrate buffer (diluted from Sigma #S6639) buffer in a 5 µm ÜberStrainer (pluriSelect) ...
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bioRxiv - Cancer Biology 2021Quote: ... the remainder of the solution following removal of aqueous phase was mixed with 300ul of Back extraction buffer (4M Guanidine Thiocyanate, 50mM Sodium Citrate dihydrate, 1M Tris free base, and molecular grade water. All reagents from Sigma Aldrich) per 1ml of triazol ...
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bioRxiv - Bioengineering 2020Quote: ... and Ag NPs (diameter of 60 nm) synthesized from silver nitrate by reduction with sodium borohydride and stabilization with sodium citrate (all supplied by Sigma-Aldrich)[75] were utilized as prototypes of metallic NPs.
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then spun down, resuspended in 200 μL of solution A (50 mM sodium citrate, 0.1 mg/ml RNAse A (Sigma, Cat# R6513), 0.2 mg/ml Proteinase K (Thermo Scientific™ ...
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bioRxiv - Evolutionary Biology 2022Quote: ... bacterial densities were determined by plating out 100 µl of a dilution series ranging from 10−5 to 10−7 on Vibrio selective Thiosulfate Citrate Bile Sucrose Agar (TCBS) plates (Sigma Aldrich). Plates were incubated over night at 25° C and the total amount of colonies was counted the following day.
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bioRxiv - Plant Biology 2020Quote: The holdase chaperone was measured by using the cytosolic MDH1 (cytMDH1) from Arabidopsis thaliana (Huang et al., 2018) or the citrate synthase (CS) from porcine heart (Sigma-Aldrich) at 55°C or 43°C ...
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bioRxiv - Cell Biology 2019Quote: ... lead citrate and then with colloidal gold to add fiducials to both sides of the grid (10 nm gold nanoparticles, Sigma-Aldrich).
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bioRxiv - Immunology 2019Quote: ... Heat-mediated antigen retrieval was performed with 10mM sodium citrate and non-specific staining reduced by incubation in 5% (v/v) goat serum (#G9023, Sigma Aldrich) for 30 mins at RT ...
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Plasma derived cell-free mitochondrial DNA originates mainly from circulating cell-free mitochondriabioRxiv - Genomics 2021Quote: ... Anticoagulant Citrate Dextrose Solution A (ACD-A) buffer (0.1M trisodium citrate, 0.11M glucose and 0.08M citric acid) and Prostaglandin E1 (1μM) (Sigma-Aldrich, St. Quentin Fallavier Cedex) were then added to the plasma ...
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bioRxiv - Microbiology 2020Quote: ... which was added to the growth media from an Fe(III)-citrate solution prepared by mixing Fe(III)Cl3 (6 mM) and citric acid (12 mM) powders (Sigma-Aldrich) in Milli-Q water ...
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bioRxiv - Developmental Biology 2022Quote: ... Medium was refreshed 3 days after seeding and 3 days later cells were fixed with citrate/acetone/3% formaldehyde and stained with Leukocyte Alkaline Phosphatase Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: Mice were euthanized and blood was collected and immediately mixed with 1 ml 4% sodium citrate followed by addition of Histopaque-1077 (catalog no:10771, Sigma-Aldrich). This mix was centrifuged at 400 g for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... rinsed slices were incubated in 10 mM sodium citrate (pH 6.0) with 0.05 % Tween-20 (v/v, Sigma Aldrich, Taufkirchen, Germany) for 20 min at RT ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A commercial 0.02mg/ml 10nm silver nanoparticles (AgNP) in aqueous buffer with sodium citrate as stabilizer (Sigma Aldrich, Saint Louis, MO) was used for the study ...
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bioRxiv - Microbiology 2024Quote: ... collected on carbon coated copper grids (EM Resolutions, UK), and stained sequentially with 2 % aqueous uranyl acetate (BDH, UK) and 0.5 % aqueous lead citrate (Sigma-Aldrich, UK). Sections were examined and imaged in an FEI Talos F200C TEM at 200 kV.
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bioRxiv - Biochemistry 2022Quote: ... Lysate was transferred to a Teflon-capped glass tube and vortexed with an equal volume of citrate buffer saturated with phenol (Sigma-Aldrich), and heated to 80 °C for 3 h ...
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bioRxiv - Genetics 2023Quote: ... Antigen retrieval was performed by pressure cooker incubation of slides in 1L of pre-warmed 1X Citrate Buffer (Sigma Aldrich #C9999) + 0.1% Tween in dH2O for a total of 15 mins (human ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then pelleted and resuspended in 500 μL sodium citrate solution with 10 μg/mL propidium iodide (Sigma, 537059-50MG). Cell clumps were broken up by brief (3 s ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were immediately subjected to heat-mediated antigen retrieval at 95°C for 10 minutes in 1X pH6.0 sodium citrate buffer (Sigma-Aldrich, #21545) using Micromed T/T Mega Multifunctional Microwave Rapid Histoprocessor (Milestone) ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by antigen retrieval using Tris EDTA buffer pH 9 (Genemed, 10-0046) or 1x sodium citrate at pH 6 (Sigma, C9999) according to manufacturer’s recommendation for specific antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of cell culture medium were incubated at 37°C for 3 hours with 20 µL reaction mix containing sodium citrate (10 mM; pH 4.2) and 4-methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (2 mM; #474502, Sigma-Aldrich/Merck). The reaction was stopped by 5 volumes of glycine and Na2CO3 ...
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bioRxiv - Cancer Biology 2024Quote: ... two serial sections) or TILNTd (TILNTd: two serial sections) were subjected to antigen retrieval procedures with citrate buffer pH 6 (Sigma Aldrich) at 100-112 °C for 15 minutes in a TintoRetriever Pressure Cooker (BioSB ...
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bioRxiv - Genomics 2024Quote: ... 200 mM citric acid/tri-sodium citrate buffer and 1.5 % w/v lysing enzymes from Trichoderma harzianum (L1412, Sigma, now discontinued). Digestions were incubated for three hours at 30 °C with shaking at 80 RPM ...
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bioRxiv - Microbiology 2024Quote: ... nAgCSs were synthesized via the reduction of silver nitrate (AgNO3) with trisodium citrate (Na3C6H5O7; TSC) and stabilized by low molecular weight chitosan (50–190 kDa, Sigma-Aldrich). Chitosan was dissolved in 1% (vol/vol ...
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bioRxiv - Neuroscience 2021Quote: ... tissues underwent antigen retrieval by incubating for 15 minutes in hot 10mM sodium citrate buffer pH=6.0 (Sigma cat. no. S-4641). Sections were rinsed in PBS and incubated in 3% H2O2/PBS for 15 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... The gel compartment was removed after 1 to 5 days of incubation (depending on fusion) by adding 4 w/v% sodium citrate (Sigma Aldrich Inc.) for 15 min as a lyase for alginate (Supplementary figure 5 ...
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bioRxiv - Cancer Biology 2020Quote: ... After probe hybridization increasingly stringent washing steps were applied in saline-sodium citrate (SSC) buffer (Sigma, SSC 20x buffer concentrate S6639-1L) at various concentrations and temperatures ...
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bioRxiv - Neuroscience 2020Quote: ... Each sample was diluted 1/10 with McIlvaine citrate-phosphate buffer pH 4.5 and activity assayed with 4-methylumbelliferyl-2-acetamido-2-deoxy-β-D-gluco-pyranoside (Sigma, Dorset, UK) in a plate reader.
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein G was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 5ug Mouse Monoclonal anti-PIWIL1 antibody (Millipore SIGMA, Cat# SAB4200365) for 4 hours at 4°C with rotation ...
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bioRxiv - Immunology 2020Quote: ... Detection of GFP and Iba1 required high-temperature antigen unmasking in 0.01 M citrate buffer (pH 6.0) (Sigma-Aldrich, San Diego, CA). Sections were treated with biotin and streptavidin blocking solutions (Vector Laboratories ...
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bioRxiv - Genomics 2021Quote: ... Coverslips were then rinsed in 2X-SSC (1X SSC: 150mM sodium chloride, 15mM sodium citrate) 3x and incubated for >24 hrs in 50% formamide (Sigma, catalog # 47671) / 2X-SSC (pH 7.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein G was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 5ug Mouse Monoclonal anti-PIWIL1 antibody (Millipore SIGMA, Cat# SAB4200365) or 5ug Normal Mouse IgG Polyclonal Antibody (MERCK ...
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bioRxiv - Cell Biology 2024Quote: ... cells were deproteinated for 15 minutes in 0.1 N HCl and neutralized for 5 minutes in 2X Saline Sodium Citrate buffer (2X SSC) (Sigma Aldrich, Cat # S6639) at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 5x SSCT buffer was prepared by diluting 20x SSC (saline sodium citrate) in DEPC water and adding 0.1% tween (Sigma Aldrich, Cat# P1379). Next ...
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bioRxiv - Cell Biology 2020Quote: ... dark agouti rats via a single intraperitoneal injection of streptozotocin (STZ, 55 mg/kg, in trisodium citrate buffer, pH 4.5, Sigma-Aldrich Co, MO, USA), with control animals receiving an equivalent volume of vehicle ...
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bioRxiv - Molecular Biology 2020Quote: ... Up to 180mL of blood was collected into 3 60 mL syringes pre-loaded with 6 mL of anticoagulant acid citrate dextrose (ACD) (Sigma C3821 Lot#SLBW6172). Within 15 min of collection ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... subjected to heat induced antigen retrieval in a citrate buffer and incubated with a mouse anti-NeuN antibody (clone A60, Millipore, MAB377, 1:100). The used secondary antibodies were mouse linker antibody (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... Broth cultures were then streaked onto modified SCAI medium [42] which comprised of Simmons citrate agar (Oxoid, Hamphire, United Kingdom) supplemented with 1% myo-inositol (Sigma, St. Louis, USA) and amoxicillin (10 mg/L ...
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bioRxiv - Biochemistry 2020Quote: ... antigen retrieval was conducted on deparaffinised 3D epidermal skin equivalents by incubation at 95°C in citrate buffer (Sigma-Aldrich, St. Louis, USA) for 20 mins ...
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bioRxiv - Microbiology 2022Quote: ... 40 μL of 50 mM citrate buffer - pH 4.8 and 50 μL of 0.50% xylan solution (Megazyme, Ireland; Sigma-Aldrich, St. Louis, MO, USA). The reactions were incubated at 50 ° C for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... and blood was collected from the vein into an acid-citrate-dextrose buffer containing 1 µg/mL prostaglandin E1 (Sigma, St. Louis, MO). Platelets and plasma were separated from the platelet-rich plasma of blood by gel filtration ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μM-sections were deparaffinized and antigen retrieval was performed in 10 mM citrate buffer (pH = 6; Sigma-Aldrich, St Louis, MO, USA) for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... were deparaffinized and then subjected to antigen retrieval for 10 minutes at 121 uC at 15 PSI in 2.94 g/L sodium citrate (pH 6; Sigma Aldrich, 61332-04-3). Sections were permeabilized in PBS with 0.4% Triton X-100 (PBT ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were deparaffinized and immersed for 10 min in a 10 mM sodium citrate buffer at pH 6.0 (Sigma Aldrich, cat. no. C9999) at 95°C for antigen retrieval ...
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bioRxiv - Neuroscience 2024Quote: ... 24.75 µl of activity assay buffer (0.25% w/v taurocholic acid in citrate/phosphate buffer) containing 2 mM 4-methylumbelliferyl-β-D-glucopyranoside substrate (4MU-Gluc; Sigma-Aldrich, # M3633) in activity assay buffer ...
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bioRxiv - Physiology 2024Quote: ... 10-week-old male WT and Lgr4cko mice were injected with vehicle (sodium citrate, pH 4-4.5) or low-dose (40mg/kg body weight) (19, 57, 58) STZ (Sigma Aldrich, St Louis, MO) for five consecutive days ...
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bioRxiv - Cancer Biology 2021Quote: ... Radiochemical purity was determined chromatographically with instant thin-layer chromatography paper (Gelman Sciences, Ann Arbor, MI) using an acid citrate dextrose buffer (Sigma-Aldrich, St Louis, MO) as the mobile phase ...
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bioRxiv - Molecular Biology 2019Quote: ... Whole blood (3 mL) was collected by venipuncture into syringes containing acid citrate dextrose solution (ACD) (Sigma Aldrich, St. Louis, MO, USA. Cat #: C3821).