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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Mouse Putative Phospholipase B Like 2 PLBD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... mouse anti Centrin-2 (1:1000, Sigma Aldrich 04-1624), mouse anti γ-tubulin (GTU88 ...
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bioRxiv - Genomics 2020Quote: ... CD19+ B-lymphocytes and CD14+ monocytes were isolated by negative selection using RosetteSep kits (Sigma). Macrophages were produced from monocytes by culturing in RPMI with 20% fetal calf serum with 50 ng/ml M-CSF for one week as described [53] ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used in this work were: mouse anti-α-tubulin clone B-512 at 1:1500 (Sigma Aldrich), mouse anti-EB1 at 1:500 (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... blotted and immunodetected with a rat anti-CLASP2 or mouse anti-α-tubulin antibodies (clone B-512, Sigma Aldrich). For analysis of recombinant CLASP2 C-term ...
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bioRxiv - Genetics 2019Quote: ... optic vesicle-like structures were manually dissected and maintained as free floating organoids in poly HEMA (Sigma)-coated flasks with twice weekly feeding of 3D-RDM (RDM + 5% FBS (WiCell) ...
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bioRxiv - Molecular Biology 2021Quote: Tendon-like constructs were washed with ice cold PBS and lysed in RIPA buffer (Sigma-Aldrich, #R0278) supplemented with both a protease cocktail Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... FSL-B(tri) (Function-Spacer-Lipid with blood group B trisaccharide; Sigma Aldrich) or lactosylceramide (LC ...
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bioRxiv - Immunology 2023Quote: ... GC-B cells and follicle B cells were stained with Pna-HRP (Sigma) and anti-mouse IgD-BIOT (SouthernBiotech ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-vimentin (kit # 17-10195, Millipore), goat polyclonal anti-rabbit-HRP (Biorad ...
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bioRxiv - Immunology 2023Quote: ... using Mouse Kapa Genotyping kit (Merck-Millipore), primers (500 nM ...
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bioRxiv - Immunology 2020Quote: ELISA plates (Cat. M5785, Sigma Aldrich) were first coated overnight at 4°C with 10μg/ml of each B cell peptide epitope ...
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bioRxiv - Molecular Biology 2023Quote: ... Ghrelin ELISA (EZRGRA-90K)(Sigma Aldrich) IGF-1 ELISA (Catalog #80574 ...
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bioRxiv - Immunology 2022Quote: – Antibody enzyme conjugates for ELISA (Sigma): Peroxidase conjugated Anti-mouse polyvalent immunoglobulins (IgG ...
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REV-ERBα mediates complement expression and circadian regulation of microglial synaptic phagocytosisbioRxiv - Neuroscience 2020Quote: ... 2% Mouse-on-mouse (M.O.M) blocking reagent and 0.4% Triton X-100 (Sigma-Aldrich, St. Louis, MO). Sections were then incubated in TBS containing 10% goat (or donkey ...
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bioRxiv - Bioengineering 2019Quote: ... and complement C5a levels in serum were quantified by ELISA (Histamine kit: Enzo Life Sciences, Farmingdale, NY; Complement C3a kit: Quidel, San Diego, CA; Complement C5a kit: Sigma-Aldrich, St. Louis, MO). Spleen tissues were homogenized with a FastPrep-24 instrument in RIPA buffer supplemented with EGTA ...
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bioRxiv - Microbiology 2020Quote: ... The DNA fragmentation assay used the Cell Death Detection ELISA kit (initially from Roche, later Millipore-Sigma). Phosphatidylserine was detected by FITC-conjugated annexin V (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... The DNA fragmentation assay used the Cell Death Detection ELISA kit (initially from Roche, later Millipore-Sigma). Phosphatidylserine was detected by FITC-conjugated annexin V (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines tested and confirmed to be mycoplasma free using the Mycoplasma PCR ELISA kit (Sigma). CENP-A overexpression was induced by the addition of Dox to typical growth media at 10ng/ml (considered 1X) ...
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bioRxiv - Neuroscience 2021Quote: ... The BDNF of offspring hippocampus was measured using the ChemiKineTM BDNF Sandwich ELISA kit (CYT306; Chemikine, Millipore). A total of 80 µg/well of hippocampus protein was used and samples were analysed in duplicates ...
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bioRxiv - Microbiology 2021Quote: Cell proliferation activity was measured by BrdU incorporation assay using a Cell Proliferation ELISA kit (Sigma-Aldrich). The wells of 96-well plates were seeded with 5000 HSC-2 or HO-1-N-1 cells ...
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bioRxiv - Genetics 2021Quote: ... leptin and adiponectin were measured in plasma from fasted mice using ELISA kits from Millipore (EZRMI-13K), Mercodia (10-1281-01) ...
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bioRxiv - Cancer Biology 2023Quote: ... Albumin was measured in the cell culture medium by Human ALB/Serum albumin ELISA Kit (Sigma-Aldrich) and in the cell lysate by qPCR ...
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bioRxiv - Neuroscience 2024Quote: Neuropeptide Y levels in tissue were measured with commercially available ELISA kit (EMD Millipore, EZHNPY-25 K). Mice were anesthetized with isoflurane and euthanized by decapitation ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Microbiology 2021Quote: ... livers were finely minced with a sterile razor blade and digested overnight at 37°C in 40 mL 1×PBS containing 2% penicillin/streptomycin/amphotericin B (1×PBS −2% PSF) and 5 mL 0.5% clostridial collagenase solution (0.025 g of collagenase (Sigma, C0130) in 5 mL of dH20 for immediate use) ...
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bioRxiv - Plant Biology 2021Quote: Anti α-tubulin antibody (DM1A) and anti α-tubulin antibody (B-5-1-2) were purchased from Sigma-Aldrich and used at ×1,000 dilution for immunostaining (DM1A and B-5-1-2 ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were resuspended with 2% FBS in PBS for downstream B cell isolation or 10% Dimethyl sulfoxide (Sigma-Aldrich) in FBS for further preservation.
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used in this study: anti-α-tubulin (clone B-5-1-2 - Sigma, T5168); anti-acetylated histone H3 (K9 ...
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bioRxiv - Molecular Biology 2019Quote: ... at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich) at a 1:1500 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... 2% horse serum and 1% P/S) supplemented with Gibco™ B-27™ Supplement (50X, 1:50, Sigma) for a further 10 days.
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bioRxiv - Immunology 2021Quote: ... purified resting B cells were cultured at a density of 1.5-2× 106/ml in B cell culture medium supplemented with 25mg anti-IgM antibody (Millipore) and 2ng/ml of IL-4 (PeproTech ...
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bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
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bioRxiv - Microbiology 2021Quote: ... Mouse antibody isotypes were determined using the mouse monoclonal antibody isotyping kit (Sigma).
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bioRxiv - Genomics 2020Quote: ... mouse monoclonal anti-HMGB1/2 (1:1000; Sigma-Aldrich 12248-3D2); rabbit polyclonal anti-CTCF (1:500 ...
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bioRxiv - Pathology 2019Quote: ... Mouse anti-NCAM Clone 2-2b (Millipore MAB5324, RRID:AB_95211, 1:250); Rabbit anti-OCT-4 (Cell Signaling Technology 2750 ...
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bioRxiv - Cell Biology 2021Quote: ... and 12 ng/mL Fgf-2 from mouse (Sigma-Aldrich SRP3038). mESCs were passaged using StemProTM AccutaseTM (Thermo Fisher Scientific A1110501 ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human recombinant MAO-A (hMAO-A) and MAO-B (hMAO-B) enzymes (Sigma-Aldrich) were diluted in 50 mM phosphate buffer (final protein amount ...
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bioRxiv - Microbiology 2022Quote: ... purified bacterial cultures (B. adolescentis and B. fragilis at 107CFU/ml) or putrescine (Sigma) diluted to final concentrations of 33/66/100mM ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA encoding MHC heavy chain (HLA-C*08:02 and H-2 Kb) and light chain (human β2M and mouse β2M) was synthesized (Idobio) and cloned into pET-22(b) vector (Novagen). The vectors were transformed into the E ...
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bioRxiv - Cancer Biology 2024Quote: ... Following amplification cells were washed for 15 min with wash buffer B (Sigma Duolink PLA kit) and incubated with Hoechst for 5 min before another 15 min wash with buffer B ...
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bioRxiv - Genetics 2020Quote: ... Specific primers for mouse leptin and 2 mouse housekeeping genes used for normalization (β-actin and 34B4 mouse genes) were purchased from Sigma (Sigma, France). We used primers for Leptin (forward ...
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bioRxiv - Cell Biology 2019Quote: ... THP-1s were differentiated into macrophage-like cells with Phorbol 12-Myristate 13-Acetate (PMA; Sigma Aldrich, USA) at a final concentration of 100 nM for 24 hours prior to infection.
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bioRxiv - Developmental Biology 2020Quote: ... Three to six ventricle-like structures per organoid were microinjected with a solution containing 0.1% Fast Green (Sigma) in sterile PBS ...
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bioRxiv - Microbiology 2021Quote: The murine macrophage-like cell lines RAW 264.7 used in this study were maintained in Dulbecco’s Modified Eagle’s Media (Sigma-Aldrich) supplemented with 10% FCS (Fetal calf serum ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in Human Plasma-like Media (HPLM) (8) + 1% pen/strep + 5% dialyzed serum (Sigma Aldrich). For SARS-CoV-2 peptide stimulation ...
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bioRxiv - Biophysics 2021Quote: ... Cells were differentiated to be neutrophil-like with a supplement of 1.3% dimethyl sulfoxide Hybri-Max (Sigma Aldrich) at 4.5 × 105 cells/mL.
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney cell line HEK293 and mouse macrophage-like cell line RAW264.7 (purchased from ECACC) were cultured in DMEM (Dulbecco’s modified Eagle’s medium, Sigma Aldrich) with 10% FBS (Fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: ... Kidney fibroblast-like cells (COS-7) from the African green monkey Cercopithecus aethiops (Merck, Darmstadt, Germany/Sigma Aldrich, St ...