Labshake search
Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Mouse Putative Phospholipase B Like 2 PLBD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... Then cells were washed with HBSS/Ca/Mg before switching to original media containing the chymotrypsin like fluorogenic substrate LLVY-R110 (Sigma), which is cleaved by proteasome 20S core particle ...
-
bioRxiv - Neuroscience 2022Quote: The cellular viability of the brain-like tissues was tested using a lactate dehydrogenase assay (LDH) (Sigma, cat.no. MAK066-1KT) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: THP-1 cells were seeded at 5 ×105/mL in 24 well plate and differentiated to macrophage-like cells by treatment with 200 nM Phorbol 12-myristate 13-acetate (PMA; Sigma) for 18 hrs ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were grown in human plasma-like medium according to the published formulation (15) with 5% dialyzed fetal bovine serum (FBS) (Sigma) and pen/strep (Invitrogen ...
-
bioRxiv - Biophysics 2023Quote: African green monkey kidney fibroblast-like cells (COS7, Cell Lines Service GmbH, Eppelheim, #605470) were cultured in DMEM (Sigma, #D8062) containing 10% FCS (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2020Quote: ... Pellets were processed according to the NXTRACT kit (CelLytic™ NuCLEAR™ Sigma-Aldrich, Milan, Italy, protocol B), to obtain native and functional nuclear proteins ...
-
bioRxiv - Microbiology 2020Quote: ... Pellets were processed according to the B protocol of the NXTRACT kit (CelLytic™ NuCLEAR™ Sigma-Aldrich, Milan ...
-
bioRxiv - Immunology 2020Quote: ... the membranes were incubated with the appropriate horseradish-peroxidase conjugated secondary Ab (1:5000 in TBST + 2% BSA; 2 h at room temperature; anti-mouse A4416 and anti-rabbit A0545, Sigma). The immunoreactivity was detected by ECL reagents (Amersham) ...
-
bioRxiv - Bioengineering 2022Quote: ... Peanut-specific IgG1 was detected using goat anti-mouse IgG1-HRP conjugated (Southern Biotechnology Associates) and substrate TMB liquid substrate system for ELISA (Sigma-Aldrich, St. Louis, MO). The plates were read in an ELISA plate reader at 405 nM (IgE ...
-
bioRxiv - Immunology 2022Quote: ... LPS and insulin and ELISA against cardiolipin (Sigma) were performed as previously described48 ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... the ELISA substrate 3,3’,5,5’- tetramethylbenzidine (Sigma Aldrich) was used to visualise the reaction ...
-
bioRxiv - Immunology 2020Quote: ... All ELISA plates were developed with TMB (Sigma) and stopped with 1N sulfuric acid ...
-
bioRxiv - Immunology 2020Quote: ... ELISAs were developed with 3,3’,5,5’-Tetramethylbenzidine (Sigma) and optical densities were acquired with a Multiskan™FC (Thermo Scientific).
-
bioRxiv - Biophysics 2020Quote: ... ELISA signals were developed by adding TMB (Sigma) in 50 mM Na2HPO4 ...
-
bioRxiv - Genomics 2023Quote: ... Adiponectin concentrations were determined by using ELISA (Millipore). Plasma alanine transaminase (ALT ...
-
bioRxiv - Immunology 2024Quote: ... adiponectin and leptin were measured by ELISA (Millipore) following manufacturer protocol.
-
bioRxiv - Cell Biology 2024Quote: 96-well ELISA plates (Sigma-Aldrich, Cat#CLS3795) were coated with rat anti-RIPK3 (clone IH12 ...
-
bioRxiv - Cell Biology 2019Quote: ... Culture media was then exchanged with media containing cytochalasin B (Cyt-B; Sigma-Aldrich, St. Louis, MO) at a final concentration 6 µg/ml ...
-
bioRxiv - Neuroscience 2019Quote: ... anti-microtubule associated protein 2 (MAP2, 1:500, mouse monoclonal, Sigma, St.Louis, MO), GluN2B (1:1000 ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-Centrin-2 (1:250, clone 20H5, 04-1624, Merck Millipore), rabbit polyclonal anti-WDR90 (1:250 ...
-
bioRxiv - Neuroscience 2020Quote: ... and (3) GluA2 (mouse anti-glutamate receptor 2; Millipore; used at 1:2000). Cochlear pieces were incubated in species appropriate secondary antibodies coupled to Alexa Fluors in the red ...
-
bioRxiv - Microbiology 2021Quote: ... for SARS CoV-2 spike detection mouse anti-spike (Cat# ZMS1076, Sigma Aldrich) respectively ...
-
bioRxiv - Biophysics 2020Quote: ... or 2 ng/µl mouse anti-beta-tubulin primary antibody (Sigma-Aldrich, T8328), the clathrin samples were incubated with 2 ng/µl rabbit anti-clathrin primary antibody (Abcam ...
-
bioRxiv - Cell Biology 2021Quote: ... Mouse anti-actinin-2 (a7811) was purchased from Sigma Aldrich (St. Louis, MO). Rabbit anti-phosphoYAP (13008S ...
-
bioRxiv - Developmental Biology 2019Quote: ... mouse anti-activated MAP kinase (diphosphorylated ERK-1&2, 1:20; Sigma-Aldrich), and rabbit anti-Sty (1:50 ...
-
bioRxiv - Immunology 2022Quote: ... was combined with mouse anti-Map2 (#M4403, Sigma, clone HM-2, 1:100) to co-label neurons ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse anti-Tropomyosin 1 and 2 (T2780, clone name-TM311, Sigma-Aldrich, 014M4782) and mouse anti-Tropomyosin 3 (CG3 ...
-
bioRxiv - Molecular Biology 2022Quote: ... using 2 μg/ml mouse monoclonal anti-flag for ESRRB (M2 Sigma, F3165) and 1μg/ml polyclonal rabbit anti-HA for NR5A2 (Abcam ...
-
bioRxiv - Cell Biology 2023Quote: ... Mouse anti-α-actinin-2 (1:200, A7811) was purchased from Sigma Aldrich. Rabbit anti-MYH6 and MYH7 for western blotting (1:500 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2-HG production was assessed via D-2-Hydroxyglutarate (D2HG) Assay Kit (Sigma-Aldrich, USA). Paired GBM cell lines were also treated with hit inhibitors individually and in combination ...
-
bioRxiv - Cancer Biology 2021Quote: Proliferation was assessed by either manual counting with trypan blue in triplicate or with Janus Green B (Sigma Aldrich #2869-83-2) with four technical replicates read on an Epoch2 (Biotek ...
-
bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies], 2 µg/mL heparin [Sigma-Aldrich] ...
-
bioRxiv - Cell Biology 2021Quote: ... Mouse mAbs anti-Vinculin (hVIN-1) and anti-α Tubulin (clone B-5-1-2) and rabbit polyclonal anti-Paxillin (HPA051309) were from Sigma-Aldrich and were diluted 1:400 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
-
Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... HEK293T cells were seeded in a 6-well plate so that they were 70-90% confluent the next day (∼0.8x10^6 - 1x10^6 cells/well in 2 mL of standard growth media containing 10% FBS (Millipore, TMS-013-B) and 1x Penicillin-Streptomycin (Invitrogen ...
-
bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
-
bioRxiv - Immunology 2021Quote: ... and homogenized with a 2 ml dounce grinder for 5 strokes with A motor and at least 20 strokes with B motor (Sigma-Aldrich D8938). The lysates were filtered through 100 µm and 50 µm cell strainers (CellTrics filters 04-004-2328 ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were changed to mTeSR Plus medium supplemented with 115 µg/mL of Hygromycin B and 2 µg/mL of doxycycline hyclate (Sigma Aldrich #D9891). The following day ...
-
bioRxiv - Physiology 2020Quote: ... tail blood from mice on CD or following HFD was collected at indicated time and plasma was frozen until assay for total GLP-1 (Multi Species GLP-1 Total ELISA kit, Millipore) or GIP (Mouse GIP ELISA Kit ...
-
bioRxiv - Neuroscience 2022Quote: The MILLIPLEX® MAP human amyloid beta and tau magnetic bead panel 4-plex ELISA kit (Millipore, Burlington, MA, USA) and the Bio-Plex 200 System instrument (Biorad ...
-
bioRxiv - Microbiology 2019Quote: ... Gal-3 levels were measured using commercially available enzyme-linked immunosorbent assay (ELISA) kits (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2019Quote: Serum and/or plasma levels of the indicated hormones and cytokines were measured by multiplex ELISA kits (Millipore, Burlington, MA) at the High-Throughput Biology Laboratory of NYU School of Medicine.
-
bioRxiv - Cell Biology 2022Quote: ... After 24-hours BrdU was added to the media and 16h later cell proliferation was evaluated using the BrdU Cell Proliferation ELISA kit (EMD-Millipore). Colorimetric analysis was done with single channel 450nm intensity ...
-
bioRxiv - Microbiology 2024Quote: ELISAs were used to confirm a subset of cytokines identified in the antibody arrays (CCL2, CX3CL1, and Galectin-3 rat ELISA kits, Sigma) using apical or basal supernatants from individual Transwell® inserts from the same experiment than the multiplex array.
-
bioRxiv - Neuroscience 2024Quote: Serum insulin and leptin were measured by an enzyme-linked immunosorbent assay (ELISA) kit (#EZRMI-13K and #EZML-82K; Millipore). Serum triglyceride levels and total cholesterol were measured using a commercial colorimetric assay kit (LaborLab® ...
-
bioRxiv - Biochemistry 2024Quote: ... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4° C with the following primary antibodies in 1X PBS containing 2% Triton X-100 and 2% horse serum: mouse anti-NeuN (EMD Millipore), rat anti-Ki-67 (Invitrogen) ...
-
bioRxiv - Immunology 2023Quote: ... cells were stained in PBS + 0.5% BSA + 0.1% sodium azide + 2% Normal Mouse Serum (NMS) and 2% FBS (Sigma, St. Louis, MO). Rat anti-mouse purified CXCR5 (2G8 ...
-
bioRxiv - Cancer Biology 2020Quote: ... the DAC-pretreated (D3R3) cells were treated with 1 μg/mL cytochalasin B (Cyto B; C6762, Sigma-Aldrich) for 1.5 hours prior to coculture with γδ T cells.
-
bioRxiv - Immunology 2022Quote: ... cells were seeded at a density of 1 × 106 cells per well in 12-well plates and stimulated with LPS (100 ng/mL, M1-like - Sigma-Aldrich), IL4 (10 ng/mL ...