Labshake search
Citations for Millipore Sigma :
401 - 450 of 3554 citations for FGF 21 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... The other homogenization buffer was CST buffer (NaCl2 146 mM, Tris HCl pH 7,5 10 mM, CaCl2 1mM, MgCl2 21 mM, 0.49% CHAPS (Millipore-Sigma), 0.01% BSA ...
-
bioRxiv - Cell Biology 2020Quote: ... nucleation mix was prepared containing desired concentration of αβ-tubulin (3.5-21 μM) purified from bovine brain with 5% Cy5-labeled tubulin along with 1mg/ml BSA (A7906, Sigma) in assay buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... and concentrated at 21°C to 4.0 mg/ml using a 30 kDa cut-off centrifugal concentrator Amicon Ultra-15 (Millipore). Two criteria for preliminary selection for batch crystallization were getting crystals with the highest possible symmetry as well as having one Nsp10/16 heterodimer in the asymmetric unit ...
-
bioRxiv - Biochemistry 2019Quote: ... The peptide digest was dissolved in 21 μL of LC-MS grade water containing 0.1% formic acid (F0507-100ML, Sigma). The digest was filtered with a 0.65 µm pore-size micro-centrifuge filter before it was loaded to an HPLC auto-injection sample.
-
bioRxiv - Molecular Biology 2020Quote: BHK-21 cells obtained from the ATCC (LGC Standard) were maintained in Dulbecco’s modified Eagle’s Medium with glutamine (Sigma-Aldrich) supplemented with 10 % foetal calf serum (FCS) ...
-
bioRxiv - Microbiology 2019Quote: ... RIG-I/MDA5 -/- MEFs and BHK-21-BSR T7/5 cell lines were cultured in DMEM (D5796, Millipore Sigma) supplemented with 1mM sodium pyruvate (S8636 ...
-
bioRxiv - Cell Biology 2021Quote: Adult and 21 dpp wildtype and Adad2M/M testes were collected and fixed overnight in Bouin’s Solution (Sigma Aldrich). Tissue was cleared in diH2O ...
-
bioRxiv - Microbiology 2020Quote: ... Dexamethasone 21-phosphate disodium salt (DEX) (Cat# D1159-5G) and cimetidine (Cat# C4522-5G) were purchased from Sigma-Aldrich, USA ...
-
bioRxiv - Cancer Biology 2020Quote: The established murine colon carcinoma cell line MC38 (21) was maintained in Dulbecco’s Modified Eagle’s Medium Ham’s Nutrient Mixture F-12 (DMEM, Sigma-Aldrich, D8437) with 10% fetal bovine serum (Gibco) ...
-
bioRxiv - Cell Biology 2020Quote: ... RNA Polymerase inhibitors were added to cells for 24 h at the following concentrations: BMH-21 (RNAPI inhibitor; Millipore) at 1 µM ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were differentiated in osteogenic media for 14 or 21 days then treated with 10−8M 1,25(OH)2 vitamin D (Sigma) or vehicle (DMSO ...
-
bioRxiv - Microbiology 2023Quote: BHK-21 cells obtained from the ATCC (LGC Standard) were maintained in Dulbecco’s modified Eagle’s medium with glutamine (Sigma-Aldrich) supplemented with 10 % FCS ...
-
bioRxiv - Plant Biology 2019Quote: ... the membrane was incubated with anti-6x His antibody horseradish peroxidase (HRP) conjugate (Sigma A7058-1VL ...
-
bioRxiv - Molecular Biology 2019Quote: ... Supernatant containing 20mM imidazole was incubated with Ni-NTA His•Bind® Resin (Novagen) beads with end-to-end shaking for 4 hr at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... the His-tag was cleaved using a Thrombin kit (Millipore Sigma, St. Louis, MO). Confirmation of the His-tag removal was confirmed by W ...
-
bioRxiv - Microbiology 2021Quote: ... Cleared lysates were loaded on chromatographic columns filed with His-select resin (Millipore Sigma) or glutathione resin (GE healthcare) ...
-
bioRxiv - Biochemistry 2022Quote: ... Ni-NTA pull down was conducted by using HIS-Select Nickel Affinity Gels (Sigma). After lysing cell pellet with buffer A (6 M guanidine-HCl ...
-
bioRxiv - Microbiology 2021Quote: ... Target proteins were bound to 1 mL Ni-NTA His-Bind resin (Millipore Sigma) in gravity columns ...
-
bioRxiv - Biochemistry 2020Quote: ... FphF protein was initially purified by Ni2+ affinity chromatography (HIS-Select resin, Sigma-Aldrich) using an elution buffer containing 50 mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2021Quote: ... HIS-tagged B subunit of Shiga toxin 1(SML0655) and Apilimod (A149227) from Sigma; rabbit anti-EEA1 (#3288) ...
-
bioRxiv - Plant Biology 2021Quote: ... Western blotting was performed using anti His-tag (Cat no. H1029-5ML, Sigma, USA) as well as with anti GST antibody (Cat no ...
-
bioRxiv - Plant Biology 2020Quote: ... The pET-26-tNPR1-His plasmid was then transformed into Rosetta (DE3) pLysS (Novagen). The overnight culture at 37°C was transferred to fresh LB medium containing 50 μg/mL kanamycin and was incubated to OD600=1.0 at 37°C ...
-
bioRxiv - Microbiology 2019Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich) and 1% antibiotic-antimycotic (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
-
bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham, MA or Anti-HA; Sigma Aldrich, St.Louis, MO) was added to the blocking solution at 1:2,000 dilution and incubated overnight at 4 °C ...
-
bioRxiv - Plant Biology 2022Quote: ... and the subsequent western blot analyses were performed with anti-His (Sigma, Missouri, USA) or anti-FLAG antibody (Sigma ...
-
bioRxiv - Microbiology 2020Quote: ... supplemented with 10% (v/v) HI-FBS and 1% (v/v) antibiotic/antimycotic (Sigma) in a standard tissue culture incubator at 37 °C with 5% CO2 ...
-
bioRxiv - Microbiology 2020Quote: ... A horseradish peroxidase-labeled anti-His tag antibody (1:25000 dilution; Sigma-Aldrich, Poland) diluted in 5% skimmed milk / TBS-Tween (0.1% ...
-
bioRxiv - Neuroscience 2020Quote: ... The His-tag was removed by O/N incubation with TEV protease (Millipore Sigma) at 4 °C followed by negative INAC ...
-
bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich, CAD). Phosphorylated mitogen-activated protein kinases (MAPK ...
-
bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
-
bioRxiv - Immunology 2022Quote: ... The lysate was mixed gently with 50% Ni-NTA His-bind slurry (EMD Millipore) at 4:1 ratio on a shaking platform for 60 minutes at RT ...
-
bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
-
bioRxiv - Cell Biology 2019Quote: ... input was taken and 20 µl HIS-Select® HF Nickel Affinity Gel (Sigma) pre-equilibrated with Pull-Down Buffer was added to each sample and incubated on an overhead rotator for 1h at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... and the supernatant was incubated with 6 ml HIS-Select Nickel Affinity Gel (Sigma), gently agitated at 4°C for 1 h ...
-
bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
-
bioRxiv - Immunology 2020Quote: ... BM cells were cultured in growth media supplemented with 10% HI FBS (Sigma-Aldrich), 1% Pen/Strep (Lonza) ...
-
bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
-
bioRxiv - Biochemistry 2021Quote: ... the His-tag was removed by treating purified nanobody Nb_0 with carboxypeptidase A (Sigma) and B (Roche ...
-
bioRxiv - Molecular Biology 2022Quote: ... Expression of pAgos was verified by Western blot with antibodies against His-tag (Sigma) after cell growth for 2 hours at 30 °C ...
-
bioRxiv - Bioengineering 2022Quote: ... and mVenus-SpyCatcher-6×His were prepared using Gibson assembly with pET-19b (Novagen) as the plasmid vector ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mpp75Aa1.1 or Mpp75Aa1.1-His was mixed with high purity pepsin (Sigma, St. Louis, MO) in 2 mg/ml NaCl ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS-hi; Sigma, Cat. no. F7524), 1% antibiotic-antimycotic (AA ...
-
bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
-
bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
-
bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
-
bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
-
bioRxiv - Plant Biology 2020Quote: ... Extractions were dried to completeness using a rotary evaporator and then 300 µL of 21% (v/v) sodium ethoxide in ethanol (Sigma) was added and incubated for 30 minutes with gentle rocking and vortexing every five minutes and 400 µL hexane was added and vortexed for 30 seconds ...