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Citations for Millipore Sigma :
451 - 500 of 3554 citations for FGF 21 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... In vitro mineralization was analyzed by quantifying the calcium deposition at 21 day using 2% Alizarin red S (ARS, Sigma) dye as reported in literature.48 For quantification ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF7 cell lines were cultured during 14 to 21 days before Crystal violet coloration (0.01% final concentration; Sigma Aldrich #CO775) or MTT assay (1 mg/mL final concentration).
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bioRxiv - Cell Biology 2022Quote: ... cells were cultured in osteogenesis induction medium for 21 days and calcium depositions were detected by Alizarin Red staining (Sigma). Osteogenesis induction medium ...
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bioRxiv - Biophysics 2022Quote: ... equipped with variable-angle epi-illumination(21, 22) and a custom-built perforated dichroic mirror (PDM) (Sigma Koki, Tokyo, Japan). The PDM had an elliptical hole (short axis = 6 mm ...
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bioRxiv - Microbiology 2021Quote: ... and hamster BHK-21 cells (ATCC CCL-10) were grown in Dulbecco’s modified Eagle’s medium - high glucose (DMEM, Sigma-Aldrich D5671) supplemented with 10 % heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... TSA (0.6 mg/kg/day for 15 days to obtain FAPs for co-colture and for EVs isolation or 21 days for the transplant experiments; #T8552, Sigma), dissolved in DMSO solution or in DMSO alone as vehicle control (CTR) ...
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bioRxiv - Bioengineering 2021Quote: ... total DNA content was isolated from the 3D constructs at 14 and 21 days using a DNA extraction kit (Sigma). The isolated DNA contents were collected and quantified by using Nano drop (Thermo Scientific-2000).
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bioRxiv - Neuroscience 2020Quote: ... three organoids embedded in Matrigel (at day 21) and three unembedded organoids were manually dissected from transwell membranes and enzymatically digested with Accumax dissociation reagent (Sigma) with manual trituration ...
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bioRxiv - Physiology 2019Quote: ... corresponding to ATCC CRL-4003) were grown in normoxia (21% O2) in Dulbecco’s Modified Eagle’s medium (DMEM) (Sigma-Aldrich, D2906), supplemented with 10% charcoal stripped calf serum (Hyclone) ...
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bioRxiv - Microbiology 2020Quote: ... was prepared by transfecting Baby Hamster Kidney Fibroblasts (BHK-21) cells with 1μg of in vitro transcribed viral RNA with Lipofectamine LTX (Sigma Aldrich) to generate a P0 virus stock ...
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bioRxiv - Microbiology 2021Quote: Proteins were detected by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting as previously described (21) using antibodies against the Flag epitope (Sigma) or the 6xHis-tag (Bethyl Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... liposomes were extruded with the LipSoFast-Basic extruder (Avestin) 21 times through 2 polycarbonate membranes (Sigma-Aldrich; WHA10417004 or WHA110405). For testing SMA ...
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bioRxiv - Microbiology 2022Quote: ... and end of the gradient (fractions 19-21) were combined and concentrated prior to sequencing using an Amicon Ultra-0.5 30KDa filter (Millipore Sigma) [49] ...
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bioRxiv - Biophysics 2023Quote: ... 21 passes each through a 200nm and 100nm poly-carbonate Nuclepore Track-Etched Membrane (Sigma-Aldrich, St Louis, MO, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Viable single cell suspensions were resuspended in 0.04% BSA in PBS (Sigma-Aldrich, A1595-50ML and Gibco, 21-031-CV) and loaded onto the Chromium Next GEM Chip G Single Cell Kit (10x Genomics ...
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bioRxiv - Microbiology 2023Quote: ... 21 and 42 with 50 μg of recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA). Enzyme-linked immunosorbent assay was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Cancer Biology 2024Quote: K562 feeder cells were transduced with lentiviral vectors containing genes of mbIL-21 or 4-1BBL and using 8μg/mL of Polybrene (Hexadimethrine bromide, Sigma-Aldrich), in accordance with Wald Lab protocol (Dr ...
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bioRxiv - Microbiology 2020Quote: ... Human serum-supplemented medium was made through addition of complete human serum (Sigma Aldrich) into CDM to a final concentration of 5% (v/v) ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in an equal volume of human serum from human male AB plasma (Sigma) and incubated for 16 h at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 10 % heat-inactivated human serum from human male AB plasma (Sigma-Aldrich), 2.5 μM zoledronic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
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bioRxiv - Microbiology 2020Quote: ... anti-mouse (BioSupplies) or anti-His (CBM) secondary antibodies conjugated to alkaline phosphatase (Sigma Aldrich) as described in Pedersen et al35 ...
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bioRxiv - Bioengineering 2019Quote: Ni-NTA columns were prepared by packing 1 mL Ni-NTA His•Bind resins (Sigma) into a 6-ml ISOLUTE® Single Fritted Reservoir column with 10 μm polyethylene frit (Biotage ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was cleaved by overnight incubation with thrombin (from bovine plasma, Sigma Aldrich) at 4 °C during dialysis ...
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bioRxiv - Bioengineering 2020Quote: ... His tagged FLIPsuc sensors were purified by affinity chromatography using Ni-NTA bead material (Novagen). Binding to the resin was performed in batch at 4 °C for 4 h ...
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bioRxiv - Biochemistry 2020Quote: ... His-PBP1B containing fractions were pooled and treated with 2 U/mL of thrombin (Novagen) for 20 h at 4 °C during dialysis against dialysis buffer I (25 mM Tris-HCl ...
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bioRxiv - Biophysics 2020Quote: ... or 8 μM His-tagged NSP2 labelled with 8 μM Atto488-nitrilotriacetic acid (NTA, Sigma), and buffer (0.5 × phosphate saline buffer ...
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bioRxiv - Immunology 2022Quote: ... and 100 µL of alkaline phosphatase-conjugated anti-HIS tag antibody (Sigma-Aldrich, cat: A5588), diluted 1:2,000 in TBST ...
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bioRxiv - Microbiology 2021Quote: ... PfArlH or biotinylated PfArlI were subsequently mixed with HIS-Select® Nickel Affinity resin (SIGMA) while lysate of cells overexpressing Strep-SaArlI was mixed with Streptactin column material (IBA GmbH ...
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bioRxiv - Biochemistry 2021Quote: ... DTT was added to a final concentration of 0.5 mM and His-SUMO protease (Sigma) was added to a concentration 100-fold lower than the total protein concentration ...
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bioRxiv - Bioengineering 2021Quote: ... then probed with the conjugated anti-6x His tag® mouse monoclonal antibody (Sigma-Aldrich) overnight at 4ºC or 1 hour at room temperature and rewashed three times with washing buffer (CMT ...
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bioRxiv - Physiology 2020Quote: ... the N-terminal-His-tag was labeled with the Ni-NTA Atto550 fluorophore (Sigma-Aldrich) following the protocol provided by the manufacturer ...
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bioRxiv - Bioengineering 2020Quote: ... The eluted His-tagged RBD was then incubated with biotin-tagged HRV C3 protease (Sigma) and passed through a streptavidin-agarose column to deplete the protease and the 8XHis-SBP peptide ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were subjected to purification using either HIS-Select HF Nickel Affinity Gel (Sigma-Aldrich) or Glutatione Sepharose 4B (GE healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... and purified with a commercial kit according to manufacturer’s instructions (His-bind buffer kit, Millipore).
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bioRxiv - Cell Biology 2021Quote: ... and a western blot was performed using anti-HIS (Sigma H-1029, mouse, 1:3000) and anti-HA (Biolegend MMS-101R or ...
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bioRxiv - Immunology 2022Quote: ... coli and protein was isolated and purified on His-binding nickel resin (Novagen, Madison, Wisconsin). Nitrophenyl-haptenated ovalbumin (NP-OVA ...
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bioRxiv - Biochemistry 2023Quote: Strep-Full-length REC114 and His-MEI41-127 were co-expressed from pRSFDuet-1 (Novagen) and pProEXHTb (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... For super-shift assays monoclonal 0.2 μl of Anti-His antibody (H1029, Sigma-Aldrich, USA) was used ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM HEPES ...
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bioRxiv - Plant Biology 2023Quote: ... with a disruption pressure of 2.35kbar and then purified using His-bind buffer kit (Millipore) following manufacturer’s recommendations.
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bioRxiv - Biochemistry 2022Quote: ... and the supernatant was purified by affinity chromatography using Ni-NTA His-bind resin (Novagen). The column packed with the resin was equilibrated with binding buffer ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM NaPO4 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted His-tagged σNS was concentrated using a 10-kDa centrifugal filter unit (Millipore), dialyzed into 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.4 and then bound to poly-prep chromatography columns containing His-bind resin (Novagen). The yeast lysate mixture was loaded in the columns ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% v/v heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich, F7524), Penicillin-Streptomycin 100U/ml (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 1 µg/mL His-EqtII-EGFP and DAPI (D9542, SIGMA) at room temperature for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... and His-AC elution buffer were supplemented with 0.1 v/v% Tween-20 (Sigma Aldrich), 0.1 v/v% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... containing HI-2 at final concentration of 100 µM (or vehicle control DMSO, Sigma-Aldrich) was added to the cells ...
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bioRxiv - Molecular Biology 2024Quote: ... His-OGT-Flag was generated by subcloning the OGT cDNA into pET30a+ vector (Novagen®). siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic® ...