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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and 2 µg of anti-c-myc (4A6; Merck Millipore). For immunoprecipitation of PER2::Luc constructs rabbit anti-firefly luciferase IgG (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Molten (≈42°C) 2% low melting point agarose (Sigma A9414) in E3 Medium was added to the dish in an approximately 10mm-diameter droplet around the fish ...
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bioRxiv - Cell Biology 2021Quote: ... Global ROS were detected using the reporter 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA, D6883 Sigma-Aldrich). The emitted fluorescence of oxidized DFC was detected at 528 nm using the Microwin software on Mithras LB940 reader (Berthold technologies).
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bioRxiv - Molecular Biology 2020Quote: ... 7 mM 2-mercaptoethanol and 0.1% triton X-100)75 containing additional protease inhibitor cocktail (Sigma) 10uL/ml buffer ...
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bioRxiv - Microbiology 2021Quote: ... propidium iodide (PI) and 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) were bought from Sigma Aldrich (Shanghai). Ala and antifungal drugs were dissolved in DMSO to get stock solutions of 20 mM.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ROS production was measured using the fluorescent probe 2’,7’-dichlorofluorescein di-acetate (DCF; Sigma-Aldrich). Cells were seeded in 96-well black plates and ...
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bioRxiv - Plant Biology 2022Quote: ... Hydrogen peroxide contents were also analyzed using 2′,7′-dichlorofluorescein diacetate (H2DCF-DA, Sigma-Aldrich, 35845). To this end ...
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bioRxiv - Microbiology 2023Quote: ... The intracellular ROS was determined using an oxidant-sensitive probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA, Sigma) 6h post-infection ...
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bioRxiv - Microbiology 2024Quote: ... and Tuba (5’-CAGAATCATGATGAGGCCAtt-3’. These siRNAs were obtained from Sigma-Aldrich (Dyn2-2 and Dyn2-3) or Qiagen (Tuba ...
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bioRxiv - Molecular Biology 2021Quote: ... crosslinked to the membrane by chemical treatment at 60°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Sigma) for 1 h 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Dried extracts were phase-separated using -20°C LCMS-grade chloroform/methanol/water (1:3:3, v/v) (Sigma Aldrich).
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bioRxiv - Bioengineering 2021Quote: ... 7-methylguanosine (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... 7 MgCl2 (Sigma), pH 7.3-7.4 ...
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bioRxiv - Microbiology 2021Quote: ... 7-dehydrocholesterol (Sigma), and coprostanol (Cayman Chemicals ...
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bioRxiv - Neuroscience 2020Quote: ... 7 MgCl2 (Sigma), pH 7.3-7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... 7 % acrylamide (Sigma), 8 % bis-acrylamide (Sigma ...
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bioRxiv - Genetics 2020Quote: ... 7-dehydrocholestrol (Sigma) or 20-hydroxyecdysone ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 7 (Sigma), 1 mM EDTA (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... CTXφ production was induced in these strains by growing up to OD600=0.3 followed by 16 hour incubation at 37°C with aeration in LB supplemented with mitomycin C (20ng/mL) (Sigma). V ...
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bioRxiv - Biochemistry 2020Quote: ... 2-acetazolamide and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Millipore-Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... 2 mM EDTA and 1 % [v/v] protein phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich)) were added at a concentration of 2 mL/g tissue powder ...
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bioRxiv - Neuroscience 2020Quote: ... were performed with MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in Sanz et al ...
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bioRxiv - Plant Biology 2021Quote: ... Fractions were desalted on C18 Cartridges (Empore™ SPE Cartridges C18, bed I.D. 7 mm, volume 3 ml, Sigma), and reconstituted in 0.1% (v/v ...
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bioRxiv - Genetics 2020Quote: Total RNA from 7 DAP spikes was extracted using TRI Reagent and 1-bromo-3-chloropropane (BCP) (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Dried lipids were resuspended in 15µL chloroform and 65µL of click mix containing 400µM 3-Azido-7-hydroxycoumarin (Baseclick #BCFA-047-1) and 900µM tetrakis(acetonitrile)copper(I) tetrafluoroborate (Sigma #677892) in acetonitrile/ethanol (7:3 ...
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bioRxiv - Neuroscience 2020Quote: ... TodoNeo1: 5’–CCG CTT TTC TGG ATT CAT CGA C–3’from SIGMA life science) ...
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bioRxiv - Microbiology 2019Quote: ... for 5 min at 14,000 rpm at 4°C (Sigma 3-KIS centrifuge). Samples were snap-frozen in liquid N2 and delipidated by protein precipitation based on the protocol of Wessel and Flügge (61) ...
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bioRxiv - Developmental Biology 2020Quote: ... washed 3 times in M9 and frozen at −80°C in Trizol (Sigma) before RNA preparation ...
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bioRxiv - Neuroscience 2022Quote: ... and laminin for 3 hours at 37°C (5 μg/mL, Sigma L2020). To create single cell suspensions for plating aNSCs ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1 mM 8-bromoadenosine 3′,5′ cyclic monophosphate sodium salt (Sigma-Aldrich; “C”), and 0.1 mM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Developmental Biology 2023Quote: ... and blocked overnight at 4°C in DPBS with 3% BSA (Sigma, A9647) and 0.05% TritonX-100 ...
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bioRxiv - Biochemistry 2023Quote: ... A 3% (v/v) Antifoam C Emulsion solution (Sigma-Aldrich, St. Louis, MO) was added manually as needed ...
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bioRxiv - Neuroscience 2022Quote: ... recordings were performed with borosilicate glass pipettes (7-15 MΩ) and filled with normal rat Ringer’s solution with 2% biocytin (Sigma, cat # 576-19-2). The pipette was positioned with a micromanipulator (Luigs & Neumann ...
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bioRxiv - Biophysics 2021Quote: ... cells were washed with Dulbecco’s phosphate buffered saline and detached with trypsin-EDTA (7–8 min, 37 °C, 5% CO2) (Sigma). Cells were labelled with CMDiI (Thermofisher UK ...
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bioRxiv - Cancer Biology 2020Quote: ... under hypoxic conditions (7% O2) at 37°C and in mammosphere medium containing 10 nM HEPES (Sigma-Aldrich, Germany), 10 µg/ml insulin (all from PAN-Biotech ...
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bioRxiv - Cell Biology 2022Quote: ... Dead cells were excluded by 7-Amino-actinomycin (7-AAD, Sigma) staining and doublets were excluded by gating on FSC-H versus FSC-W and SSC- H versus SSC-W ...
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bioRxiv - Cell Biology 2023Quote: ... Dead cells were excluded with 7-aminoactinomycin D (7-AAD, Sigma). Cell were either sorted on AriaII or AriaIII cell sorter (BD Biosciences ...
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bioRxiv - Plant Biology 2021Quote: ... The dry residue was subjected to a double derivatization procedure with methoxyamine hydrochloride (20 mg mL−1 in pyridine, Sigma) and N-Methyl-N-(trimethylsilyl)trifluoroacetamide (Macherey-Nagel) ...
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bioRxiv - Cancer Biology 2022Quote: ... using an Agilent 7890B-5977A GC-MSD in EI mode after derivatization of twice methanol-washed dried extracts by the addition (a) for polar metabolites of 20 μL of a 20 mg/mL solution of methoxyamine hydrochloride in pyridine (both Sigma) at room temperature for >16 hr and 20 μL BSTFA + 1% TMCS (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... and derivatized by the addition of 150 μL of pyridine and 150 μL of N,O-bis(trimethylsilyl)trifluoro-acetamide with trimethylchlorosilane (99 : 1, w/w, Sigma) and incubated at 70 °C for 45 min ...
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bioRxiv - Cell Biology 2020Quote: ... Dried samples were re-suspended in 5 µL of a freshly prepared (20 mg/mL) solution of methoxyamine in pyridine (Sigma) to perform the methoxyamination ...
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... Ketone groups of metabolites were derivatized by adding with 20 μL of 30 mg/mL methoxyamine in pyridine (Sigma-Aldrich) and incubating at 37°C for 90 minutes with shaking (1000 RPM) ...
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bioRxiv - Plant Biology 2019Quote: ... The dried non-polar phase was resuspended in 70 μL of pyridine and derivatized with 30 μL of MTBSTFA + 0.01 % t-BDMCS (Sigma-Aldrich). Following transfer to a glass vial ...
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bioRxiv - Molecular Biology 2019Quote: ... Initially the samples were methoximated by incubating the samples with methoxyamine hydrochloride (25 µL, 20mg/mL in pyridine, Sigma Aldrich) at 45 °C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... The dry residues were suspended in 10 μL of O-methoxylamine hydrochloride (30 mg mL−1 in anhydrous pyridine, both from Milllipore Sigma) and incubated while mixing (1000 RPM ...
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bioRxiv - Microbiology 2024Quote: ... the media samples underwent two consecutive derivatisation procedures – oximation (25 mg.mL-1 of methoxyamine hydrochloride in anhydrous pyridine hydrochloride, Sigma-Aldrich) and silylation ...
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bioRxiv - Immunology 2024Quote: ... dried metabolite extracts were derivatized with equal amounts of methoxylamine (20 mg/mL in pyridine, both Sigma-Aldrich, Steinheim, Germany) and MSTFA or MTBSTFA (Restek Corporation ...
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bioRxiv - Genetics 2022Quote: ... Positive interactions were scored by the appearance of white-coloured colonies on a synthetic defined medium containing 6 μg mL−1 adenine and/ or by the growth in presence of 2 mM 3-Amino-1,2,4-triazole (3-AT) (A8056, Sigma).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bixafen, (N-(3’,4’-dichloro-5-fluorobiphenyl-2-yl)-3-(difluoromethyl)-1-methylpyrazole-4-carboxamide) (Bixafen, (Sigma-Aldrich, St ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 2 mM of 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC, 22980-Sigma-Aldrich, St. Louis, MO, USA) and 5 mM of N-hydroxysulfosuccinimide sodium salt (NHS ...