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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... were cleaned by sonication with 3 M NaOH then Piranha solution (2:3 ratio of 30% (w/w) H2O2 to sulfuric acid) and treated with 3-glycidyloxypropyl trimethoxysilane (GOPTS) (Sigma). GOPTS was then reacted with a mixture of 9 parts α-hydroxy-ω-amino PEG 3000 to 1 part α-biotinyl-ω-amino PEG 3000 by weight (Rapp Polymere) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in PBS containing 0.5% normal donkey serum and 0.03% Triton X-100 for 1 h at room temperature (RT, 23 ± 7□) and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2 ...
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bioRxiv - Neuroscience 2022Quote: ... Three to five weeks after reprogramming, single colonies were picked and expanded on SNL76/7 feeder cells (ATCC, SCRC-1049) treated with mitomycin C (Sigma Aldrich) in 20% KSR medium [92] ...
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bioRxiv - Immunology 2021Quote: ... and 2 ml Flt-3L-containing supernatant [produced from SP2/0 transfected cell line secreting mouse recombinant Flt-3L42 for 7 days at 37°C until activation with 100 ng/mL LPS (Sigma-Aldrich) 18 hours prior to the assays.
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were rinsed with PBS and stained according to manufacturer’s protocol (Millipore #BG-6-B, #BG-7-B, #BG-8-C). Embryos were then fixed again in 4% PFA (in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... were carefully dissected 7 to 16 days after induction of GAL4 expression at 29°C in Shield and Sang M3 medium (Sigma S3652), keeping the crop ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM EDTA) at 4 °C for 10 min before 3% of Ficoll-400 (Sigma) was added and assembled Nucleolin complexes were resolved on a 10% TBE PAGE gel at 4 °C.
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bioRxiv - Immunology 2021Quote: ... and washed by ultracentrifugation at 4°C using a 3 kDa filter (UFC900324, Merck-Millipore) to remove imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... then 85°C for 10 minutes and transfected in a 3:1 polyethylenimine (PEI, Sigma):DNA ratio into 70% confluent HEK 293A cells (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested (5,000 x g; 20 min; 4°C; Sigma 3-16KL; rotor 11180); the spent medium was TCA-precipitated (20% w/v ...
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bioRxiv - Immunology 2021Quote: ... or 400µg NP-PCC (4-hydroxy-3-nitrophenylacetyl (Biosearch) conjugated to pigeon cytochrome C (Sigma)) mixed with adjuvant based on Monophosphoryl Lipid A ...
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bioRxiv - Neuroscience 2021Quote: ... These coverslips were left to grow at 37°C and 5% CO2 and were treated with 2 µl Ara-C (Sigma) on day in vitro 1 (DIV1 ...
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bioRxiv - Genomics 2022Quote: ... Cross-links were reversed by incubating the eluted samples and inputs at 42°C for 2 hours and 67°C for 6 hours in the presence of Pronase (Sigma) and TE buffer ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were reduced at 37 °C for 1 hour with 10 mm Tris(2-carboxyethyl)phosphine (TCEP, C-4706 Sigma) in 100 mm NH4HCO3 ...
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bioRxiv - Immunology 2019Quote: Cell viability was monitored by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT; Sigma, USA) assay as previously described [52] ...
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bioRxiv - Molecular Biology 2019Quote: ... the collagenase inhibitor Ilomastat ((R)-N′-Hydroxy-N-[(S)-2-indol-3-yl-1-(methylcarbamoyl)ethyl]-2-isobutylsuccinamide) (25μM, CC1010, Merck Millipore) or a combination of both ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with 50 µL of a 2 mg/mL solution of (3-(4,5-dimethyl-thiazole-2-yl)-2,5-biphenyl tetrazolium (MTT, Sigma-Aldrich) in PBS for 4 h and then exposed to 100 µL dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS and 3-(4,5-dimethyl-2-thiazolyl)2,5-diphenyl-2-H-tetrazolium bromide (MTT, Sigma-Aldrich) solution (final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Thin-layer chromatography (solvent system chloroform : methanol : 4.2N ammonium hydroxide 9:7:2) was done as described (111) on aluminium silica TLC plates (Sigma) for 75 minutes ...
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bioRxiv - Cell Biology 2021Quote: Cellular ROS was measured by incubating cells with 100 µM 2′,7′-Dichlorofluorescin diacetate (DCFH-DA) (D6883, Sigma-Aldrich) in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Cancer Biology 2020Quote: Intracellular ROS levels were measured by monitoring the oxidation of cell permeable 2′,7′-dichlorofluorescein diacetate (DCFHDA, Sigma-Aldrich) to fluoros-pectrophotomete at excitation and emission wavelengths of 480 and 535 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... Three-mL liquid cultures were inoculated with a single colony and supplemented with 2′,7′-dichlorodihydrofluorescein diacetate (DCFHDA; Sigma) with a final concentration of 1 μM ...
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bioRxiv - Neuroscience 2022Quote: ... SDS lysate was centrifuged at 20,000 x g for 30 minutes and supernatants were subsequently diluted with 7 volumes of 1.15% Triton-X 100 in TNE with Phosphatase Inhibitor Cocktail 1 and 2 (Sigma) to produce a mixed-micelle lysate with final detergent concentrations of 0.125% SDS and 1% Triton-X 100 ...
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bioRxiv - Biochemistry 2023Quote: ... Merck; 1 mM CaCl2, Sigma; 2 mM MgCl2, Merck; adjusted to pH 7 with HCl; 0.1% Tween 20, Sigma) overnight at 4 °C ...
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bioRxiv - Genetics 2023Quote: ... 30 hours post-fertilization old embryos were exposed to 20 μM 2′,7′-dichlorofluorescein diacetate (DCFH-DA, Sigma-Aldrich) for 1 hour at 28.5 °C in the dark followed by washing with embryo water a minimum of three times 31 ...
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bioRxiv - Immunology 2023Quote: ROS production in BMDMs was measured using the membrane-permeable fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFHDA) (Sigma, #D6883). BMDMs were plated in 96-well plates at 1×105 cells/well ...
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bioRxiv - Microbiology 2023Quote: ... Once the cultures reached OD 0.1 then 108 CFU/ml of each strain was incubated with 10µM of 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Sigma) in 1xPBS (pH 7.2 ...
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bioRxiv - Cell Biology 2023Quote: ... strains containing a degron tag were arrested at 30 °C for 3 hours (total) and then treated with 250 μM 3-indole acetic acid (Sigma-Alrich, St. Louis, MO) for 30 minutes before harvesting ...
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bioRxiv - Genomics 2021Quote: ... A1413301)-coated 12-well plates at a density in the range of 3-7×105 cells per well in iPS-Brew + 2μM Thiazovivin (Sigma, cat. SML1045-5MG). Alternatively ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared for NMR by combining 16 µL standard with 50 µL serum and placing in a 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich).
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bioRxiv - Pharmacology and Toxicology 2021Quote: Methyl methanesulphonate (MMS) (#129925) (CAS registry number 66-27-3) and carbendazim (#378674) (CAS no. 10605-21-7) were purchased from Sigma-Aldrich (Merck), UK.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 3 and 7 post-drug or vehicle administration were analyzed to quantify urea levels with a urea assay kit (Sigma-Aldrich, MAK006) according to vendor-provided protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The following day membranes were washed 3-times with TBST for 7 min and incubated with corresponding solutions of anti-mouse (A9044, Sigma; 1:15,000) or anti-rabbit (A9169 ...
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bioRxiv - Neuroscience 2021Quote: ... treated with poly-d-lysine(Sigma-Aldrich A-003-E, 1-2 hr at 37°C, 2 μL/well) followed by laminin (Thermo Fisher 23017015 ...
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bioRxiv - Immunology 2023Quote: ... human peripheral blood-derived cells were stained with 7-aminoactinomycin D (7-AAD, Sigma) or LIVE/DEAD® viability markers (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 150 mg/L sodium 4-methyl-2-oxovalerate (Sigma K0629) ...
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bioRxiv - Biochemistry 2022Quote: ... Dimethyl LV labeling for geminal pair determination used 150 mg/L 2-Keto-(3-methyl-13C)-butyric-4-13C,3-d acid sodium salt (Sigma 589063). Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887 ...
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bioRxiv - Biochemistry 2022Quote: ... 13C-ILV labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 70 mg/L 2-Ketobutryic acid-4-13C,3,3,d2 sodium salt hydrate (Sigma 589276 ...
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bioRxiv - Biochemistry 2021Quote: The competitive inhibition of human fumarase activity in the presence of 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910) was fluorometrically assessed using a coupled enzyme assay ...
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bioRxiv - Plant Biology 2020Quote: ... 2-3-week-old plants were treated with 150µM DCMU (D2425-100G, Sigma), kept for 1 hour in the dark and then exposed to high light (HL ...
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bioRxiv - Cell Biology 2020Quote: ... TRCN0000037282 (shRNA Trim39#2) and TRCN0000438509 (shRNA Trim39#3) were from Sigma-Aldrich. Lentiviral particles were produced as previously described (Iréna Lassot et al. ...
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bioRxiv - Cell Biology 2019Quote: ... and 2 mg/ml N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) (Sigma, 03450) in 50 mM HEPES were added on top of the hydrogels and incubated for 30 min at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% DMSO and 300 μM 3-maleimidobenzoic acid N-hydroxysuccinimide ester (MBS; Sigma) dissolved in microtubule stabilization buffer specific for algae (MTB ...
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bioRxiv - Neuroscience 2021Quote: ... MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] were acquired from Sigma (USA). Purified water was prepared with a Milli-Q purification system from Millipore (Milford ...
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bioRxiv - Molecular Biology 2021Quote: ... selected for 2-3 weeks with 1 µg/ml of puromycin (Sigma- Aldrich), and surviving cells were fixed with methanol and stained with Giemsa (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mg/ml N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) (Sigma-Aldrich, 03450) diluted in 50 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich) added fresh] or RIPA buffer [25 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... 0.75 mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma) was added to culture medium for 2 h ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mg/ml N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (Sigma, catalog number 03450) in 50 mM Hepes] followed by 10 min UV-light activation ...
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bioRxiv - Neuroscience 2020Quote: ... and (3) GluA2 (mouse anti-glutamate receptor 2; Millipore; used at 1:2000). Cochlear pieces were incubated in species appropriate secondary antibodies coupled to Alexa Fluors in the red ...