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Citations for Millipore Sigma :
4401 - 4450 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the human cardiomyocyte cell line AC16 (#SCC109) was purchased from Millipore, and HEK 293TN (LV900A-1 ...
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bioRxiv - Bioengineering 2023Quote: ... with 1% human platelet poor-derived serum (HS; Sigma-Aldrich, P2918), 20 ng/mL basic FGF (bFGF ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... human specific N terminal anti-collagen VII LH7.2 (1:250 Millipore), The fluorescence images were taken using the TCS SP5 confocal laser scanning microscope (Leica) ...
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bioRxiv - Genetics 2022Quote: ... containing to a final concentration human serum 5% (Sigma H4522-100mL), heparin 2IU/ml (Sigma H3149-25KU) ...
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bioRxiv - Immunology 2023Quote: ... The human lymphoblastoid line BOLETH was obtained from Sigma (#88052031-1VL). B-cell lines were cultured in GlutaMAXTM supplemented RPMI 1640 medium further modified with 10% FBS ...
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bioRxiv - Bioengineering 2023Quote: ... stained using mouse anti-human fibronectin monoclonal antibody (Sigma-Aldrich, US) and detected with goat anti-mouse Alexa Fluor 488 secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and fibronectin from human plasma (Sigma-Aldrich F1056; 1 μg/ml). For some experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... and human samples were cryopreserved in 10% DMSO (Sigma-Aldrich #F4135) in FBS in liquid nitrogen at -196°C until use ...
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bioRxiv - Cancer Biology 2023Quote: ... PC3 human cell line was obtained from Sigma Aldrich (90112714-1VL) and cultured in DMEM medium with 10 % FBS.
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bioRxiv - Cancer Biology 2023Quote: PNT2 human cell line was purchased from Sigma Aldrich (95012613-1VL) and cultured in RPMI 1640 containing 2 mM Glutamine and 10 % Foetal Bovine Serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... containing 1% Human Platelet Poor Plasma-derived Serum (SH, Sigma, P2918), 20 ng/mL FGF2 and 10 uM All-trans Retinoic Acid (RA ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated on 20 μg/ml Human plasma fibronectin (Millipore) coated plates ...
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bioRxiv - Microbiology 2023Quote: ... then in rabbit anti-human IgM (Sigma 270A, IgG-coated beads) or fresh mouse serum (C3bi-coated beads) ...
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bioRxiv - Immunology 2023Quote: ... and mouse anti-human IgG4 biotin (Sigma-Aldrich, St Louis, MO), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... human plasma fibronectin (1mg/mL overnight at 37◦C; FC010, Millipore) or adhesion protein ...
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bioRxiv - Cell Biology 2023Quote: ... 1% PS) with freshly added 8 ng/mL human bFGF (Sigma). The medium was changed daily ...
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bioRxiv - Cell Biology 2023Quote: Human bronchial epithelial 16HBE14o- cells (Sigma-Aldrich, Cat. #SCC150, Poznan, Poland) were obtained as previously described (15,18,19) ...
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bioRxiv - Immunology 2024Quote: ... or isotype-control human IgG Ab (10 μg/ml; Sigma-Aldrich) was incubated on de-paraffinized tissue sections overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Plates were coated with FN (Sigma-Aldrich, Darmstadt, Germany, human plasma) and 5x105 cells were seeded and allowed to attach 5-10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% (v/v) heat-inactivated human male AB serum (Sigma-Aldrich) and 6,000 IU/mL recombinant human IL-2 (rhIL-2 ...
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bioRxiv - Cell Biology 2024Quote: ... The coverslips were coated with either 1x Fibronectin human plasma (Sigma) or poly-D-lysine (20 µg/mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... fibrinogen (final conc. of 7.35 mg/mL, from human plasma, Sigma), thrombin (from human plasma ...
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bioRxiv - Immunology 2024Quote: Human umbilical vein endothelial cells (HUVECs, #CRL-1730™, Sigma-Aldrich) were maintained in Endothelial Cell Growth Medium (#211-500 ...
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bioRxiv - Biochemistry 2024Quote: 16HBE14o- (human bronchial airway epithelial cells) were purchased from EMD Millipore Corporation (Temecula ...
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bioRxiv - Biochemistry 2024Quote: ... and human CNS tissues were extracted with RIPA lysis buffer (Millipore) containing the protease/phosphatase inhibitor cocktail (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Human cell line samples: mouse anti-β-Actin (Sigma-Aldrich, A1978); rabbit anti-PARP (Cell Signalling Technology ...
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bioRxiv - Immunology 2024Quote: Human renal proximal tubule epithelial cells (RPTECs, SA7K clone, MTOX1030; Sigma) were cultured on PureCol-coated T75 flasks (431464U ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured with Human Mammary Epithelial Cell Growth Medium (Sigma-Aldrich). HeLa (ATCC ...
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bioRxiv - Neuroscience 2024Quote: ... blocked in 1% human serum albumin in TBS (HSA; Sigma-Aldrich) containing 0.05% Triton X-100 (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... and FGF (Fibroblast growth factor basic human, Sigma-Aldrich, Cat#SRP6159) at 37°C ...
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bioRxiv - Immunology 2024Quote: ... 4 μl/ml of human fibronectin (1 mg/ml; Sigma-Aldrich), and 10 μl of 1 N NaOH (Sigma-Aldrich ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... Recombinant rMSPDHA and rMSPDHB were then expressed through 1.0 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction and purified using Ni-NTA His-Bind® Resin Kit (Novagen, San Diego, USA). The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Genomics 2022Quote: ... or T-175 flasks (Hi-C, CUT&RUN) at a density of 1 × 105 cells/mL and treated with 25 nM PMA (Sigma-Aldrich, cat # P1585-1MG). After 24h ...
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bioRxiv - Neuroscience 2022Quote: ... and triethyl amine were made in MilliQ ultrapure water (18 megaohm resistance) and 1-octen-3-ol was diluted in paraffin oil (EMD Millipore #PX0045-3). Aliquots of 10 µL of each compound and dilution was pipetted onto a piece of filter paper (5 × 20 mm ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3 times with PBS and incubated with an anti-active Caspase-3 antibody (EMD Millipore, Billerica, MA, 1:200) for 2h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... they were anaesthetized in 3-amino benzoic acid ethyl ester (Tricaine/ethyl 3-aminobenzoate; Sigma Aldrich; 168 μg·ml−1 in Tris pH 7) and embedded in 1% low melting point agarose (UltraPure Agarose ...
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bioRxiv - Neuroscience 2020Quote: ... N- (Piperidin-1-yl)-5- (4-iodophenyl)-1- (2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 3 mg/kg; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Genetics 2021Quote: ... Mouse neural progenitor cells were routinely passaged 1:2-1:4 every 3-5 days using Accutase and maintained in NS expansion medium on laminin-coated plates (Sigma, 3 μg/ml). Both mESCs and mNPCs were incubated at 37 °C and 5% CO2 ...
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bioRxiv - Genetics 2020Quote: ... containing either tunicamycin only (Enzo Life-Sciences; 2-3 μg/mL) or tunicamycin and auxin (IAA, indole-3-acetic acid, Sigma-Aldrich; 1 mM), and seeded with OP50-1 bacteria ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was mounted on charged slides and dried prior to ethanol washes (70%, 80%, 95%, 100% Ethanol, 3 minutes each) and washed in a histological clearing agent (Xylene substitute; Sigma Aldrich; 3 minutes), and then cover slipped with DPX mounting agent (Sigma Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... The excess fluorescent probe was removed through molecular weight and the buffer was exchanged with PBS (pH 7.4) using 3 kDa centrifugal filter units (UFC500396, Amicon® Ultra – 0.5mL, 3 kDa, Sigma-Aldrich®, Dublin, Ireland). Absorbance was measured at 555 and 280 nm for all tagged samples and calculations were performed according to manufacturer’s instructions using an arbitrary extinction coefficient of 100,000 and molecular mass of 100,000 SI unit ...
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bioRxiv - Biophysics 2022Quote: ... SPR2 DNA encoding residues 649-864 was generated using the polymerase chain reaction method (primers: 5’-GGCAGGACCCATATGGGCAGGAGAGGGTGGGATAATAAAGC-3’ and 5’-GCCGAGCCTGAATTCTTACTTGTCGAACTGTTGGAGATCGATTTC-3’) and individually sub-cloned into pET28 (Millipore Sigma, Burlington, MA) using engineered NdeI and EcoRI restriction endonuclease sites ...
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bioRxiv - Microbiology 2024Quote: ... The chromatic mutants were mixed in different ratios (1:1, 1:3, 3:1) and the mixtures were fixed using 4% paraformaldehyde (Sigma−Aldrich/Merck, Germany) for 4 h at 4°C.
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bioRxiv - Cancer Biology 2021Quote: ... Kinase assays were performed as described in (20) using 200ng of purified EPHA2 recombinant protein (OriGen Technologies) or indicated concentration of purified Enolase (Sigma Aldrich), in the absence or presence of about 50ng of purified Src-GFP (or Src3A-GFP as indicated ...
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bioRxiv - Evolutionary Biology 2022Quote: ... blocked in 10% NGS in PBST at 4°C for 3 hr and immunohistochemistry steps were carried out by incubating the samples with primary anti-pH3 antibody (diluted 1:300 in blocking buffer, REF 05-817R, clone 63-1C-8, recombinant rabbit monoclonal antibody, Millipore, USA) for overnight at 4°C as previously described [37–39] ...
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bioRxiv - Cell Biology 2021Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was cut in two (one half for the commercial and one half for the recombinant anti-FLAG™-M2) and each half was probed with either commercial (Sigma) or recombinant anti-FLAG™-M2 at 1 µg/mL in PBS for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... both Bactrian camel and llama AEC cultures were treated with recombinant universal type I and III IFNs (100 IU/ml; Sigma Aldrich) or recombinant type III IFN (100 ng/ml ...