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Citations for Millipore Sigma :
4201 - 4250 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... anti-neurofilament (NF200) (Sigma, #N4142, 1:800, 3 days), anti-IBA4 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... oocytes were fixed in 3% glutaraldehyde (G5882, Sigma Aldrich) in 0.1M cacodylate buffer (C0250 ...
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bioRxiv - Plant Biology 2024Quote: ... L-methionine sulfoximine (MSX; Sigma, Germany; 0, 3 µM). Salt concentrations were set at 0 mM for controls and 135 mM for treatments unless noted otherwise ...
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bioRxiv - Plant Biology 2024Quote: ... and monoclonal anti-Actin (clone 10B 3, Sigma-Aldrich) were used with their respective HRP-secondary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... injected with 2 ml of 3% thioglycollate (Sigma-Aldrich), to obtain intraperitoneal macrophages (macrophage purity > 95% ...
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bioRxiv - Neuroscience 2023Quote: ... and 3 µg/ml glucose oxidase (#G7141; Millipore Sigma) in 0.1M TB ...
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bioRxiv - Neuroscience 2024Quote: ... 200 μL of chloroform (Sigma-Aldrich, 67-66-3) was added to each tube and vortexed ...
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bioRxiv - Neuroscience 2024Quote: ... For PL pS1292-LRRK2 (Figures 1 and 3, Sigma) intensity analysis ...
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bioRxiv - Microbiology 2024Quote: ... After washing with PBS by 3 kDa ultrafiltration (Millipore), the sulfhydryl-activated tanezumab reacted with maleimide-modified AuQCs by the hetero-bifunctional sulfo-SMCC for 2 h in the dark at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 3 × 105 cells were seeded on fibronectin (Sigma #F1141)-coated 18 mm coverslips in 12-well plates and transfected with the pHLsec-αSyn plasmid (kind gift by Radu Aricescu ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were incubated with 3% Normal Horse Serum (Sigma) for 45 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were selected in 3 µg/mL puromycin (Sigma) for 48 hours and single cell colonies were isolated ...
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bioRxiv - Cell Biology 2024Quote: ... 500 nM 3-isobutyl-1-methylxanthine (IBMX, Sigma-Aldrich), 1 mM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... 3 µg/ml glucose oxidase (Cat# G2133, Sigma-Aldrich), 2 mg/ml (D+)-glucose (Cat# G5767 ...
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bioRxiv - Cell Biology 2024Quote: ... 5-bromo-2-deoxyuridine (Sigma Aldrich #59-14-3) was added to prevent the proliferation of remaining fibroblasts.
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bioRxiv - Cancer Biology 2024Quote: ... phosphatase inhibitor 2 and 3 (Sigma Aldrich; P5726and P0044). Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227) ...
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bioRxiv - Bioengineering 2024Quote: ... 0.5 mM 3-isobutyl-1-methylxanthine (Sigma, I-5879), 0.5 μM rosiglitazone (Wako ...
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bioRxiv - Bioengineering 2024Quote: ... 0.1 mM 3-isobutyl-1-methylxanthine (IBMX, I5879; Sigma), 5 μM rosiglitazone (ROG ...
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bioRxiv - Biophysics 2024Quote: ... and 3-(maleimido)propionic acid N-hydroxysuccinimide ester (Sigma). For co-grafted brushes ...
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bioRxiv - Neuroscience 2024Quote: ... 3% (v/v) Triton X-100 (Millipore Sigma, X100) was added to tissue homogenates and allowed to incubate in ice for 30 minutes with gentle shaking followed by centrifugation at 10,000xg for 15 minutes at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 3 mmol MgCl2·6H2O (Sigma, St. Louis, MI M2670), 2.5 mmol EGTA (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was blocked using 3% BSA (Millipore Sigma) in TBS with 0.1% Tween 20 (TBS-T ...
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bioRxiv - Bioengineering 2024Quote: ... 500 μM 3-isobutyl-1-methylxanthine (Sigma-Aldrich, #I5879) and 100 μM indomethacin (Sigma-Aldrich ...
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bioRxiv - Pathology 2024Quote: ... and then incubated in the 3% hydrogen peroxide (Sigma) solution in PBS and washed with PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3 μM CHIR99021 (Sigma Aldrich, Cat. No. 361571). The culture plates were coated with 0.1% gelatin (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... a 5% solution of 3-Aminopropyltriethoxysilane (APTES, Sigma-Aldrich) in tetrahydrofuran (THF ...
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bioRxiv - Microbiology 2024Quote: ... Followed by addition of 0.15% 3-hydroxydiphenol (Sigma-Aldrich), the mixture was analyzed by the absorbance at 520_nm ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5mM 3-isobutyl-1-methylxanthine (IBMX; Sigma-Aldrich, USA), 5µg/ml insulin (SAFC ...
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bioRxiv - Cell Biology 2021Quote: ... at a concentration of 1:500 was used to detect myc tagged ATR and incubated at room temperature for 1 h in blocking buffer (1 % BSA [Sigma], 0.2 % Tween-20 in 1x PBS) in a wet chamber ...
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bioRxiv - Biochemistry 2021Quote: ... and purified using DDDDK-tagged protein purification gel (MBL, Japan) followed by elution with 0.1 mg/ml FLAG Peptide (Sigma-Aldrich Co., St. Louis, Missouri, the US). To remove the flag peptide ...
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bioRxiv - Plant Biology 2021Quote: ... We prepared InsP7 by using 100ng of Mouse IP6K1 (mIP6K1) recombinant protein along with 100 µM of InsP6 (Sigma, USA) in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... T cells were then infected with HIVBaL and cultured for up to 7 days in RPMI medium additionally supplemented with 10 U/ml recombinant interleukin-2 (Sigma). The dividing cell lines HEK293T (ATCC ...
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bioRxiv - Epidemiology 2019Quote: ... and recombinant heat labile toxin β subunit protein [15,52,53] from enterotoxigenic Escherichia coli (ETEC LT β subunit) were purchased from Sigma Chemical (St ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse recombinant EphrinB1-Fc chimera (cat # E0653-200UG) and bovine serum albumin (cat #05470-1G) with purity >98 % were from Sigma. Alexa Fuor-594 BSA (cat #A13101) ...
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bioRxiv - Bioengineering 2021Quote: ... Columns were washed with 20 column volumes PBS and recombinant proteins were eluted with 100 mM β-d-lactose (Sigma) prepared in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without recombinant enzyme Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). Of note ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were overexpressed in E.coli BL21 (DE3) at 17 °C overnight and then purified by Glutathione-agarose (Sigma #G4510). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... Sample was then diluted 8-fold with 50 mM HEPES pH 8.0 and digested by the addition of 5 μg TrypZean recombinant trypsin (Sigma-Aldrich) and incubation for 8 hours at 30 °C ...
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bioRxiv - Plant Biology 2021Quote: ... residual trypsin activity was measured by monitoring the change in absorbance at 247 nm in presence of increasing concentration of recombinant purified Kunitz Trypsin inhibitor (KTI) when incubated with p-toluene-sulfonyl-L-Arg methyl ester (Sigma). To study the inhibitory action of P235 on KTI action ...
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bioRxiv - Plant Biology 2021Quote: ... Substrate specificity of recombinant AtPME2 activity was determined at pH 7.5 and 28°C using commercial citrus pectin (Sigma-Aldrich, DM >85% ...
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bioRxiv - Systems Biology 2020Quote: ... At time 0 medium was removed from cells and recombinant mouse tumor necrosis factor alpha (TNF-α, Sigma-Aldrich, #T7539) was added at concentrations 0-100 ng/mL as 5-minute pulse ...
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bioRxiv - Microbiology 2021Quote: Six-week-old Balb/cN mice were primed subcutaneously either with 30 μg of recombinant SARS-Cov-2 Spike protein (S) or Spike-RBD (RBD) in the complete Freund’s adjuvant (SIGMA-ALDRICH) and boosted three times at three-week intervals with 20 μg of the same antigen in the incomplete Freund`s adjuvant ...
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bioRxiv - Plant Biology 2021Quote: ... substrate specificity assays for recombinant PcIBMS1 and PcIPMS1 were determined with a spectrophotometric end-point assay with DTNB (Sigma-Aldrich). An aliquot of 1-5 μL of the protein preparation was added to an enzyme assay mixture (100 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... and fractions judged by SDS-PAGE to contain the recombinant protein were pooled and desalted by size exclusion chromatography using 30K centrifugal spin column (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... recombinant proteins were purified by anti-c-Myc Antibody Beads (10D11; Wako) or Anti-FLAG M2 affinity gel (Sigma-Aldrich) following the standard protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the recombinant protein were pooled and concentrated through 15 mL 10-kDa MWCO Amicon-Ultra centrifugal filters (Millipore). Protein concentrations were measured by absorbance at 280 nm in nanodrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... pFPV-TurboFP650 recombinant plasmids were selected on Trypticase Soya Agar (TSA - BioMérieux) containing 100 µg/mL of carbenicillin (Sigma-Aldrich) and clones which showed a purple color were selected for restriction analysis ...
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bioRxiv - Microbiology 2021Quote: ... The rabbit polyclonal anti‐Per1 antibody was generated against recombinant Per1 protein (recPer1) corresponding to bases 55‐462 of per1 CDS (Aste010406) expressed in pET‐42a (Novagen) commercially (GL Biochem Ltd ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml recombinant IL-1β (Preprotech) or 100 ng/ml lipopolysaccharide from Escherichia coli O55:B5 (LPS, Sigma-Aldrich) for 6h at 37°C ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... For capture of antibodies and Fc-Fusions a CM5-S-Series sensor chip (Cytiva) was functionalized with 2500RU recombinant Protein A (Sigma) using EDC/NHS Coupling Kit (Biacore/Cytiva BR-1000-50 ...