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Citations for Millipore Sigma :
4351 - 4400 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... a control group received injection of recombinant fusion proteins (3.8 μM) mixed with complete Freund adjuvant (CFA, Sigma) in 50 μl of PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 250-500 μg of recombinant proteins were resuspended in HAT buffer supplemented with 0.75 mM acetyl CoA (Sigma) and 1 μl butyric acid (200 mM ...
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bioRxiv - Microbiology 2022Quote: ... Assay was conducted according to the manufacturer’s instructions and recombinant L-Lactic Dehydrogenase from porcine heart (Sigma-Aldrich) was used to generate a standard curve ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1 IU/mL insulin and 10 ng/mL human recombinant epidermal growth factor (EGF) (all from Sigma-Aldrich). TKCC10 cells were grown in a 1:1 mixture of M199 medium and Ham’s F12 medium ...
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bioRxiv - Cell Biology 2023Quote: ... either coated with BSA or recombinant Laminin332 (BioLamina LN332-0502, diluted in Dulbecco’s Phosphate Buffered Saline, Sigma, D8662) and left to adhere overnight ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with medium containing 10 µg/mL recombinant human adiponectin (SRP4901, Sigma-Aldrich, in sterile water), a quantity equivalent to typical adiponectin levels in lean women (Ding et al. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Bioengineering 2023Quote: ... mESC maintenance media was supplemented with 1,000 units/mL of Recombinant Mouse Leukemia Inhibitory Factor (Millipore Sigma # ESG1107). Media was changed daily.
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were purified using a Ni-NTA column (Yeasen, Wuhan, China) and concentrated by ultrafiltration (Millipore). Protein concentration was determined by SDS-PAGE using BSA as a standard.
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bioRxiv - Cell Biology 2023Quote: 2 μg GST-tagged NuSAP point mutants were incubated with 50 ng human recombinant Aurora A (Sigma-Aldrich) in a water bath at 30°C for 30 min in kinase buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were expanded in the presence of 10 U/mL recombinant human IL-2 (11147528001, Sigma). Human CD8+ T cells were purified from donor PBMCs (NHSBT or Karolinska Hospital ...
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bioRxiv - Biochemistry 2023Quote: ... and the recombinant protein was eluted using buffer D supplemented with 10 mM reduced L-glutathione (Sigma–Aldrich). The purified protein was digested with TEV protease overnight ...
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bioRxiv - Biochemistry 2023Quote: His-PP-Pex6 1-204 protein (purified by recombinant expression from plasmid pDC69) was digested with 0.1 or 0.2 μg/μL trypsin protease (Sigma) for 10 minutes at 23 °C in 100 mM Tris pH 8.0 buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Supplemented IVM media consisted of MediCult IVM media (Origio) supplemented with 75 mIU/mL of recombinant FSH (Millipore), 100 mIU/mL recombinant hCG (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... The recombinant APC/C complex was eluted with the wash buffer supplemented with 2.5 mM desthiobiotin (Sigma-Aldrich). Before loading the elution fractions onto a 6 mL Resource Q anion-exchange column (Cytiva) ...
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bioRxiv - Physiology 2024Quote: ... were incubated in hypoxia condition in the hypoxia chamber with TGFβ1 (recombinant human TGFβ1: 15ng/ml, Cat #T7039, Sigma)25 for 72 h ...
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bioRxiv - Physiology 2024Quote: ... were incubated in hypoxia condition in the hypoxia chamber with TGFβ1 (recombinant human TGFβ1: 15ng/ml, Cat # T7039, Sigma)10 for 72 h ...
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bioRxiv - Microbiology 2024Quote: ... with 50 μg of either recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA) for NF10 production or with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... (Piperno and Fuller 1985)), mouse-anti-β-tubulin (E7; at 1:100; Developmental Hybridoma Studies Bank, University of Iowa) and rabbit-anti-Zwf1 (Sigma A9521; at 1:10,000), followed by goat-anti-mouse-680 (LI-COR 926-68070 ...
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bioRxiv - Neuroscience 2021Quote: ... After three washes in PBS sections were incubated with secondary antibodies (anti-Rabbit IgG CF™ 568, 1:1000, Sigma Aldrich, SAB4600084, and Anti-Guinea Pig CF™ 647, 1:1000, Sigma Aldrich, SAB4600180) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... for 30 min at RT followed by 1 h incubation at RT with rabbit anti-FLAG antibody (1:500, Sigma-Aldrich, St. Louis, MO, USA) and mouse anti-SEC31A antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... we used donkey anti-goat Igg H&L (Alexa Fluor® 647) Preadsorbed (Ab150135, 1:50) and sheep anti-rabbit Cy3 (Sigma–Aldrich C2306, 1:100).
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bioRxiv - Cell Biology 2022Quote: ... Corresponding secondary HRP-conjugated anti-rabbit (1:3000 or 1:2000 in 5% non-fat milk or 5% BSA/TBS-T; Sigma-Aldrich, St. Louis, MO, USA) was used for a 1-hour incubation at room temperature ...
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bioRxiv - Pathology 2024Quote: ... buffer the membranes were incubated for 1 hour at room temperature with anti-rabbit/mouse/goat IgG secondary antibody conjugated with horseradish peroxidase (1:5000; Sigma–Aldrich, St Louis, MO, USA). Signals was detected with Clarity enhanced chemiluminescence (ECL ...
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bioRxiv - Bioengineering 2023Quote: ... pH 7.4) for 1 h at room temperature and were subsequently incubated with primary antibodies rabbit anti-claudin-5 (Sigma-Aldrich, cat# SAB4502981, AB_10753223, 1:300), mouse anti-claudin-5 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit α-MORN1 (kindly provided by Dr. Marc-Jan Gubbels, Boston College, MA; 1:100) and mouse α-Centrin1 (clone 20H5; Millipore Sigma; 1:100). Stained gels were washed three times with PBST (1xPBS + 0.1% Tween20 ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-AGO1-N-coil (1:1000 in 5% milk in PBS-T) followed by anti-Rabbit antibody (1:10,000 [Sigma] in 5% milk in PBS-T). The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T) ...
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bioRxiv - Neuroscience 2023Quote: ... First antibody (Guinea pig anti-Fos, 1:500 dilution, Synaptic Systems, Cat. # 226005; Rabbit anti-Esr1, 1:3000 dilution, Millipore, Cat. # 06-935, Lot # 3243424) was diluted in PBST with 3% normal donkey serum and incubated overnight (12-16 hours ...
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bioRxiv - Microbiology 2023Quote: ... After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301, Goat α-mouse HRP 1:1000– Sigma Aldrich/GERPN4401), revealed using ECL reagent (BioRad ...
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bioRxiv - Developmental Biology 2024Quote: ... containing 5% skim milk for 1 hour and incubated at 4 °C overnight with antibodies including α-c-Myc produced in rabbit (Sigma-Aldrich, C3956, 1:2,000), and α-HA high affinity produced in rat (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... rabbit anti-NDC80 (dilution 1:1000) (Oliveira et al. 2024) and mouse anti αtubulin (dilution 1:100, Sigma-Aldrich, St. Louis, MO; catalog number T6199). Antibodies against KNL1 and NDC80 were originally developed using respective peptides EDHFFGPVSPSFIRPGRLSDC and EQGINARDAERMKRELQALEG from Cuscuta sp ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... and −7173 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Bioengineering 2024Quote: ... Washes were followed by the addition of 100 μL of anti-FLAG horseradish peroxidase (HRP)-linked monoclonal antibody (mAb, #A8592, Sigma Aldrich, St. Louis, MO) for 1 h at room temperature with rocking ...
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bioRxiv - Microbiology 2024Quote: ... and -71 60 of anti-spike murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2023Quote: ... of anti-spike murine antibodies (including cross-reactive mAbs against SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2023Quote: ... and -71)60 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-BiP (Cat#: G9043) and rabbit anti-Stx17 (Cat#: HPA001204) antibodies from Sigma, rabbit anti-RTN4/NOGO (Cat# ...
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bioRxiv - Immunology 2022Quote: ... from BD Biosciences, α-fibronectin (rabbit, polyclonal) and α-rabbit IgG-AlexaFluor546 (polyclonal) from Sigma-Aldrich. CellTracker Green CMFDA Dye was used from Thermo Fisher.
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-GPSM2 (Ezan et al., 2013) and rabbit anti-GNAI3 (#G4040, Sigma-Aldrich). After washing in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... or rabbit IgG (PP64; Millipore) at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-ATG5 (Sigma-Aldrich), rabbit anti-ATG9A (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-MIB1 (Sigma, M5948) at 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-KIAA0753 (Sigma, HPA023494) at 1:1000,rabbit anti-CCDC14 (Genetex ...
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bioRxiv - Cell Biology 2020Quote: ... anti-rabbit GFP (Sigma g1544), anti-mouse Flag (Sigma F3165) ...
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bioRxiv - Genetics 2021Quote: ... or rabbit anti-Flag (Sigma) antibodies and precipitated proteins were analyzed by western blot using mouse anti-HA (Sigma ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-Flag (Sigma, SAB4301135), mouse anti-m5C antibody (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... coupled to rabbit IgG (Sigma), prepared as previously described (Oeffinger et al ...