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Citations for Millipore Sigma :
4301 - 4350 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Cells were stained with rabbit anti-GFP (2.5 µg/ml) and mouse DM1α (1:500, Sigma-Aldrich T6199), rabbit anti-Kif18B serum (1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... ubiquitin carboxyl-terminal esterase L1 (UCHL1) (29) using rabbit anti-human UCHL1 (Sigma, HPA005993 at 1:1,000 dilution). The secondary antibody was Alexa Fluor 594-conjugated goat anti-rabbit (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP (AP182P, Merck Millipore) or donkey anti-mouse IgG HRP (AP192P ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies were detected with anti-rabbit or anti-mouse IgG secondary antibodies conjugated to HRP (1:80,000; Sigma). Antigen-antibody complexes were visualized using enhanced chemiluminescence substrate (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... The following antibodies were used in this study: rabbit anti RNF168 polyclonal Ab (1:1000 dilution, Millipore, ABE367), mouse anti myc mAb (1:1000 dilution ...
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bioRxiv - Physiology 2022Quote: ... Slides were incubated with polyclonal rabbit primary anti-BPGM antibody (1:200, Sigma-Aldrich, Cat. No. HPA016493, RRID:AB_1845414), followed by incubation with an HRP anti-Rabbit secondary antibody (1:100 ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... washed with Binding Buffer and incubated with biotinylated anti-rabbit antibody in Binding Buffer (1:10,000, Sigma-Aldrich). After washing with Binding Buffer ...
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bioRxiv - Biophysics 2024Quote: ... Next day membranes were incubated with peroxidase conjugated anti-rabbit IgG raised in Goat (Sigma, 1:8000 dil.) and peroxidase conjugated anti-mouse IgG raised in Rabbit (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... and sections incubated overnight at 4°C under gentle agitation: rabbit anti-CaMKIIα (Millipore, #05-532, 1:200), guinea pig anti-Parvalbumin (Synaptic Systems ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were reprobed with the anti-actin 20-33 rabbit polyclonal antibody (1:10,000 dilution; Sigma Aldrich #A5060). For comparison of protease-resistant α-syn banding patterns ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were subsequently incubated overnight with a rabbit anti-TH antibody (1:2000, AB152, Millipore Sigma, USA), a rat anti-DAT antibody (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... and a biotinylated donkey anti-rabbit immunoglobulin G secondary antibody (1:1,000 for 2 hours at RT; Millipore). To eliminate nonspecific binding prior to immunostaining endogenous peroxidase activity was blocked using 0.3% H2O2 for 30 min at room temperature (RT) ...
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bioRxiv - Bioengineering 2023Quote: ... then membranes were incubated overnight at 4 °C with rabbit anti-BCRP (Sigma-Aldrich, cat# ZRB1217, 1:1000) or mouse anti-active β-catenin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated overnight at 4°C with the following primary antibodies: rabbit anti-TH (Millipore, 1:1000), mouse anti-TPH (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... These coverslips were afterwards incubated with the following primary antibodies: rabbit anti-SUN1 (1:200; HPA008346; Sigma-Aldrich), rabbit anti-SUN2 (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated with primary antibody rabbit anti-c-fos (1:1000, Merck Millipore, Burlington, United States) in TBS (5% BSA ...
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bioRxiv - Pathology 2023Quote: ... and then incubated with biotinylated secondary antibody goat anti-rabbit (1:400; Sigma-Aldrich Cat#B8895, RRID: AB_258649), in TBS-Sap for 90 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-rabbit IgG (H+L) Highly Cross-Adsorbed CF®594 (Sigma-Aldrich, Cat#SAB4600110, 1:1000), goat anti-mouse IgG Abberior STAR 635P(Abberior Cat# ST635P-1001-500UG ...
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bioRxiv - Microbiology 2023Quote: ... followed by secondary antibodies goat anti-rabbit IgG horseradish peroxidase (1:10,000 dilution, Sigma-Aldrich, Cat. #12-348) or goat anti-mouse IgG horseradish peroxidase (1:10,000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with HRP-conjugated goat anti-rabbit IgG (1:20,000) and developed using 0.2 mg/mL TMB (Sigma), 0.015% H2O2 in 100 mM sodium acetate ...
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bioRxiv - Developmental Biology 2023Quote: ... Phosphorylated myosin II was detected using a primary rabbit anti-phosphomyosin antibody (Sigma-Aldrich, F3648; 1/200 dilution). Incubation with primary antibodies was performed overnight at 4°C in TBS-T containing 0.1% Triton-X and 5% goat serum in 1xTBS at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The DPLA probe anti-rabbit plus binds to the TKS4 primary antibody (HPA036471, Sigma Aldrich, 1:100 dilution), whereas the DPLA probe anti-mouse minus binds to the CD2AP antibody (clone 2A2.1 ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation was performed on total-protein lysate using 1 μg of rabbit polyclonal TTC29 antibody (HPA061473, Sigma-Aldrich) and 15 μl of Protein A/G magnetics beads (Bio-Ademtec ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were subsequently stained with mouse or rabbit anti-HA antibody (Sigma-Aldrich H3663 or H6908, 1:1000) and the following Golgi markers ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-TRF1 (gift from Titia de Lange) and mouse anti-α-tubulin (1: 5,000) (Sigma-Aldrich #T6199) at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... for half an hour before incubation in the primary anti-GABA antibody (rabbit, dilution 1:1000, A2052; Sigma) for 48 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBS-Tween20 (0.1%) and incubated with secondary-HRP conjugated antibodies (1:10.000; Anti Rabbit IgG HRPO, Millipore BI2407 ...
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bioRxiv - Neuroscience 2024Quote: ... PrPC blots were reprobed with the rabbit polyclonal anti-actin antibody 20-33 (1:10,000; Millipore Sigma #A5060).
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bioRxiv - Molecular Biology 2023Quote: ... The following rabbit polyclonal antibodies were used at a 1:500 dilution: anti-HNRNPF (Sigma-Aldrich, cat# HPA069667); anti-HNRNPK (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with biotinylated secondary antibody goat anti-rabbit (1:400; Sigma-Aldrich Cat#B8895, RRID: AB_258649) in TBS-Sap ...
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bioRxiv - Cell Biology 2023Quote: ... CUT&RUN was performed on HC11 cells using a rabbit anti-VANGL2 antibody (1:50, Millipore Sigma, ABN2242) and isotype control IgG (CST ...
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bioRxiv - Plant Biology 2024Quote: ... Commercial alkaline phosphatase (AP)-conjugated goat anti-rabbit (IgG) antibodies (Sigma-Aldrich, USA, diluted 1:5,000 in PBS) were added and incubated for 2 h at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The following primary antibodies were used with the indicated dilutions: rabbit anti-LHPP (Sigma Millipore ZRB1050, 1:250), rabbit anti-human vimentin (Abcam ab8069 ...
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bioRxiv - Cancer Biology 2024Quote: ... The following primary antibodies were used with the indicated dilutions: rabbit anti-LHPP (Sigma Millipore ZRB1050, 1:250), rabbit anti-human vimentin (Abcam ab8069 ...
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bioRxiv - Cell Biology 2024Quote: ... The ear tissue sections were incubated overnight with anti-phospho-Smad1/5/9 antibody (Millipore, AB3848-1, rabbit), anti-Ki67 antibody (Abcam ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Biochemistry 2019Quote: 25 µl of 100 µM recombinant purified GST Pat1-NC was bound to 30 µl GST-slurry (Sigma), and one aliquot of GST-beads was included without added Pat1-NC as negative control ...
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bioRxiv - Immunology 2019Quote: ... supplemented with cytokine cocktails aimed at proliferation (50 ng/ml murine recombinant thrombopoietin (TPO, Sigma-Aldrich SRP3236-10UG) and 100ng/ml Stem Cell Factor (SCF) ...
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bioRxiv - Immunology 2019Quote: ... NMRI mice were injected subcutaneously at two different locations with 100µg of the recombinant Thio-EmACT resuspended in 100µl Freund Incomplete adjuvant (Sigma). The double injections were repeated four weeks later to boost the mice anti-EmACT response ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant protein was then isolated from the supernatant by nickel affinity chromatography using loose Ni-NTA resin (Sigma). Protein eluted from Ni-NTA resin was filtered and then loaded onto a size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2021Quote: ... FAK) recombinant proteins to be labeled with Alexa fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant FLAG-tagged zebrafish Grk1a and Grk1b were each inserted into the pFLAG-CMV2 vector (#E7033, Millipore-Sigma). HEK-293 cells cultured in DME/F-12 with 10% fetal bovine serum were transfected with either of these constructs using FuGENE 6 Transfection Reagent (#E2691 ...
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bioRxiv - Bioengineering 2022Quote: ... Epidermal growth factor (EGF) and Recombinant Human Fibroblast Growth Factor Basic (FGF2) Progesterone were purchased from Sigma-Aldrich. OPSS-PEG-SVA (3400 Da) ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant FLAG-tagged zebrafish Grk1a and Grk1b were each inserted into the pFLAG-CMV2 vector (#E7033, Millipore-Sigma). HEK-293 cells cultured in DME/F-12 with 10% fetal bovine serum were transfected with either of these constructs using FuGENE 6 Transfection Reagent (#E2691 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentration of total protein in CFE with recombinant Scaf19LKT was determined by Bradford reagent (Sigma-Aldrich, USA), CFE was diluted 1,000-times in 0.1 M sodium carbonate coating buffer (pH 9.0) ...
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Immunology 2020Quote: ... Transduced CD8+ T cells were expanded in the presence of 10 U/ml recombinant human IL-2 (Sigma) Human CD8+ T cells were purified from donor PBMCs (Cambridge Bioscience or NHSBT ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... cells that were cultured in 0.5 % FBS were stimulated with hEGF (recombinant EGF) (Sigma Alrdich, Catalog No: E9644) in concentrations (10 and 30 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... except for the control group for which recombinant fusion proteins were mixed with incomplete Freund adjuvant (IFA, Sigma). Blood samples were collected from the submandibular vein at different time points for more than 6 months and sera were prepared and analysed for the presence of specific antibodies by ELISA as described below ...