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Citations for Millipore Sigma :
4301 - 4350 of 10000+ citations for Urotensin 2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Fur was submerged in 2 mL of 2:1 (v/v) chloroform:methanol (Sigma 288306 and Sigma 322415) followed by sonication in a water bath for six minutes to dislodge lipids ...
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bioRxiv - Microbiology 2023Quote: ... Fur was submerged in 2 mL of 2:1 (v/v) chloroform:methanol (Sigma 288306 and Sigma 322415) followed by sonication in a water bath for six minutes to dislodge lipids ...
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bioRxiv - Neuroscience 2023Quote: Mice were anaesthetized with avertin (2,2,2-tribromoethanol 1.25%; 2-methyl-2-butanol 0.78%, 20 ll/g, ip, Sigma-Aldrich) before bilateral injection of AAV on a stereotaxic frame (David Kopf Instruments) ...
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bioRxiv - Neuroscience 2023Quote: ... Grids were then placed in a 2% solution of poly(2-hydroxyethyl methacrylate) (Sigma-Aldrich, P3932-10G) in ethanol to create a hydrophobic coating ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets were resuspended in 200µl of LCMS-grade acetonitrile/methanol/water (2:2:1) (Sigma Aldrich) chilled to -20°C and transferred to lysing matrix Y tubes (MP Biomedicals ...
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bioRxiv - Cell Biology 2023Quote: Cells on coverslips were fixed at Day 2 by immersion in 2% (v/v) paraformaldehyde (Sigma-Aldrich) made up in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... with the addition of 2.5 mg/mL inhibitor of WNT production-2 (IWP-2, I0536, Sigma-Aldrich) for the last 3 days ...
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bioRxiv - Bioengineering 2023Quote: ... along with 5% 2-hydroxy-1-[4(hydyroxyethoxy)phenyl]-2- methyl-1-propane (Irgacure 2959; Sigma-Aldrich) photoinitation ...
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bioRxiv - Cancer Biology 2023Quote: ... the RetroNectin solution was replaced by 2 mL of sterile 2% BSA solution (Sigma Aldrich, cat#4503) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were anesthetized with 2.5% Avertin73 (2,2,2-tribromoethanol, Sigma T48402, solubilized in 2-Methyl-2-butanol, Sigma 152463 ...
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bioRxiv - Microbiology 2023Quote: ... 4 µl internal standard (2 g/l L-2-aminoadipic acid, Sigma-Aldrich Chemie GmbH, Buchs, Switzerland), and 3.5 µl diethyl ethoxymethylenemalonate (VWR International AG ...
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bioRxiv - Biochemistry 2023Quote: ... Media from the DMS group was aspirated and replaced with 2 mL of a 2% DMS (Sigma) solution in cell media (prepared by brief vortexing to incorporate DMS) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 ml ion exchange cartridge packed with 100 mg of 2– 2(pyridyl)ethyl silica gel (Sigma) was activated with 1 ml of methanol and equilibrated with 1 ml of buffer A (methanol/H2O 1:1 ...
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bioRxiv - Cell Biology 2024Quote: ... 2-Desoxy-D-glucose (2-DG) was used at final concentration of 1mM (Sigma Aldrich, ref. D3179). Aminooxyacetic acid (AOA ...
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bioRxiv - Cell Biology 2024Quote: ... 3 day old transgenic animals were anaesthetized on 2% agarose pads containing 2 mM levamisole (Sigma, L9756). DAF-28::GFP expression pattern was examined in at least 30 animals on day 3 of adulthood ...
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bioRxiv - Cell Biology 2024Quote: ... 2-month-old Tx-CreGrk2F/F mice were injected with 2 mg of tamoxifen (#T2859, Sigma Aldrich) in corn oil (#C8267 ...
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Biostimulation methods based on chemical communication improve semen quality in male breeder rabbitsbioRxiv - Animal Behavior and Cognition 2024Quote: The 2-phenoxyethanol solution was prepared by diluting 2-phenoxyethanol (PHR 1121, Sigma- Aldrich, St. Louis, USA) to a concentration of 1% in 25% ethanol ...
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bioRxiv - Cell Biology 2024Quote: ... They were subsequently postfixed in 2% OsO4 and 2% K4Fe(CN)6 (Sigma-Aldrich, St. Louis, MO) in 0.1M sodium cacodylate buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... the plate was blocked with 2 mL of 2% bovine serum albumin (Sigma Aldrich 9048-46-8) in DPBS and washed with 2 mL of HBSS (Sigma Aldrich H6648) ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody was incubated at room temperature protected from light for 1 hour in Intercept®(TBS) Protein-Free Blocking Buffer with 0.01% Tween®20 (Sigma, St. Louis, MO) using IRDye® 800CW Goat anti-Mouse IgG secondary antibody at 1:10,000 (LI-COR Lincoln ...
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bioRxiv - Cell Biology 2021Quote: ... Primary and secondary antibody incubations were performed for 1 hour at 25°C in blocking buffer with anti-Flag-M1 (Sigma-Aldrich, #F3040, 1:1000) conjugated with Alexa-647 (Molecular Probes ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane is incubated for 1 h in a blocking buffer of PBST (PBS supplemented with 0.1 v/v % Tween) and 10% (v/w) dry skim milk (Sigma-Aldrich, St. Louis, MO, USA). The membrane is washed three times with PBST for 5 min and then incubated for 1 h at 25 C with a primary anti-dynein antibody (sc-13524 ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in blocking solution with the following primary antibody: 1:1000 mouse anti S-100 β subunit (S2532, Sigma-Aldrich, St. Louis, MO). Sections were washed and then incubated for 2h in blocking solution with the following secondary antibody at a 1:500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were further incubated at room temperature for 1 h with a blocking buffer (3% normal donkey serum; S30, Chemicon by EMD Millipore, Billerica, MA, USA) in PBS solution containing 0.2% Triton X-100 and 0.05% Tween 20 (PBST) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... prepared in antibody blocking solution containing 0.5% BSA (Fisher, Pittsburgh PA, USA #BP9703-100) and 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA #T9284) for 48-72 hours at 4°C in a shaker ...
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bioRxiv - Neuroscience 2019Quote: ... following by primary antibody incubation overnight at 4°C (rabbit anti-pRIPK3, Abcam; mouse anti-TH, Millipore; mouse anti-pMLKL: Millipore rabbit anti-TH, Millipore in blocking/permeabilizing solution). Sections were washed in 0.1M TBS and then incubated in secondary antibodies for 2 hours at RT in TBS (goat anti-rabbit Alexa Fluor 488 ...
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bioRxiv - Genomics 2021Quote: ... and nucleolin (D4C7O) rabbit mAb (Cell Signalling cat # 14574) appropriately diluted in Odyssey® Blocking Buffer (TBS) 0.1% TWEEN® 20 (Sigma Aldrich cat # P1379) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... in 50 μL of blocking buffer were added for 1 hour followed by 50 μL ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5000 dilution) in blocking buffer for 1 hour at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: ... and then blocked for 1 hour in blocking buffer (1% BSA and 10% normal donkey serum, 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA). Primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... the calcium free medium was replaced with the cell culture medium with or without E-cadherin blocking monoclonal antibody DECMA-1 (Millipore, 10 µg mL−1), and cells migration was carried out for 4 hours at 37°C in a 5% CO2 humidified incubator.
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bioRxiv - Genetics 2022Quote: ... Membranes were washed three times with TBST and incubated with blocking buffer containing Rabbit IgG HRP Linked Whole Ab (Sigma cat no. GENA934-1ML) at 1:5000 for two hours with gentle mixing ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in primary antibodies (diluted in the blocking solution, see Table 1) against myc (rabbit c-Myc, 1:1000, Sigma-Aldrich, C3956, RRID: AB_439680) and FLAG (mouse-FLAG ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in blocking solution: rabbit anti-GFP (MBL Cat# 598; 1/300) and mouse anti-HA (Sigma, Cat# 12ca5; 1/50). On the next day ...
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bioRxiv - Genetics 2024Quote: ... by transferring them in a small drop of blocking solution to the surface of a 0.01% poly-L-lysine (Sigma Aldrich, Cat# 25988-63-0) coated slide ...
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bioRxiv - Microbiology 2023Quote: ... After cell permeabilization and blocking with 1% normal goat serum (Nacalai Tesque) in dilution buffer [0.3% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) in PBS containing 3% bovine serum albumin (Nacalai Tesque)] ...
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bioRxiv - Cell Biology 2022Quote: ... Nitrocellulose membranes were blocked for 1 h with the blocking buffer containing 5 % dried milk in TBS-Tween 1 % and incubated over-night with primary antibodies: anti-αTubulin (Sigma, T6074, 1:1000 dilution), anti-acetylated Tubulin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... in 50 μl of blocking buffer were added for 1 h followed by 50 μl ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5.000 dilution) in blocking buffer for 1 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... After blocking for 1 hour in blocking buffer (for Fig. 1D: 5% Protifar; for Suppl. Fig. 2B: 0.2% v/v Fish skin gelatin (Sigma–Aldrich, St. Louis, MO, USA)+ 0.1% v/v Tween–20 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated in blocking buffer for 1 hr at room temperature followed by incubation with rabbit anti-RBM10 (Sigma-Aldrich HPA034972, 1:2000), rabbit anti-HDAC1 (GeneTex GTX100513 ...
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bioRxiv - Neuroscience 2024Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). Following five 5-minute washes at 20°C with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The immunoprecipitates on protein A agarose or sepharose were either boiled directly in sample buffer or eluted with FLAG peptide (Sigma, 200 μg/mL) or HA peptide (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The selected mouse and rabbit immunoglobulins were affinity purified on protein G SepFast resins (BioToolmics, UK) or on peptide used for immunization and conjugated to a cyanogen bromide-activated-Sepharose® 4B (Sigma-Aldrich, USA), respectively ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... BG-oligonucleotide-labeled SNAP proteins bound to resin were again washed with wash buffer and eluted using 0.2 mg/mL FLAG peptide (Sigma-Aldrich, St. Louis, MO) overnight at 4°C with rotation ...
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bioRxiv - Cancer Biology 2021Quote: ... Beads were washed 3 times with lysis buffer and TFEB or TFE3 eluted using 200 ng/μL 3X-flag-peptide (Sigma-Aldrich, F4799-4MG).
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bioRxiv - Biochemistry 2020Quote: ... isolated from the BBPHs were added to the reaction mix before adding the enzyme to assess the inhibition potential of the peptides and the results have been compared with a standard ACE inhibitor Lisinopril (L2777, Sigma-Aldrich, MO, USA), used as positive control (50 µL of 2 µg/mL).
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bioRxiv - Immunology 2020Quote: ... and in vitro cultured for 18-24 hours in media supplemented with or without peptide at 0.5 µM (or, as positive control, with 1 µg/ml Staphylococcal enterotoxin B (SEB, Sigma Aldrich, St. Louis, USA)) ...
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bioRxiv - Molecular Biology 2021Quote: ... Bead-particle complexes were washed once with 200 µl washing buffer (20 mM Tris-HCl pH 7.5 and 150 mM NaCl) and subsequently eluted with FLAG peptide (30 min at 37 °C; 200 μg/ml in washing buffer, Sigma Cat. No. F3290) and lysed by addition of Amphipol A8–35 (Anatrace ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
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bioRxiv - Cell Biology 2021Quote: The peptide/ZO1 interaction was detected in situ using the Duolink secondary antibodies and detection kit (Sigma, St Louis, MO, Cat: 92002, 92004) according to manufacturer instructions ...