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Citations for Millipore Sigma :
4251 - 4300 of 10000+ citations for Urotensin 2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 2 μg/ml doxycycline hyclate (Sigma), 2 μM XAV939 (Cayman Chemical) ...
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bioRxiv - Neuroscience 2024Quote: ... 5-Fluoro-2’-deoxyuridine (Sigma-Aldrich) was typically added at a final concentration of 16 uM at DIV 7–9 to limit non-neuronal cell growth ...
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bioRxiv - Cell Biology 2024Quote: ... Phosphatase Inhibitor Cocktail 2 (P5726, Sigma) and Cocktail 3 (P0044 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 2 mM L-glutamine (Merck Millipore) and 1% penicillin/streptomycin (Merck Millipore ...
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bioRxiv - Developmental Biology 2024Quote: ... Large dextran molecules (2 MDa, Sigma) were dissolved in growth medium in varying concentrations to generate varying osmotic pressures82 ...
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bioRxiv - Genetics 2024Quote: ... 2 μg/mL heparin (Sigma-Aldrich) and Primocin (InvivoGen) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 2% (w/v) Bacto Peptone (Sigma), 2% glucose (Astral) ...
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bioRxiv - Molecular Biology 2020Quote: ... The following antibodies used for immunocytochemistry as described above are as follows: mouse monoclonal antibody to FLAG peptide (Sigma #F1804, 1:500), sheep polyclonal to TGN46 (Biorad #AHP500G ...
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bioRxiv - Molecular Biology 2020Quote: The following antibodies used for immunoblot as described above are as follows: mouse monoclonal antibody to FLAG peptide (Sigma #F1804, 1:5000), Rabbit monoclonal to c-Myc (abcam #ab32072 ...
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bioRxiv - Bioengineering 2019Quote: ... Hela cells from the same batch of 2D culture were seeded into the peptide hydrogel with density ~ 8 × 104 which was measured by an automated cell counter (Millipore Corporation, PHCC3000). The 3D culture was maintained using a 24-well cell culture plate with ~ 390 μL cell suspension ...
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bioRxiv - Cancer Biology 2021Quote: ... and calmodulin-binding peptide (CBP) was affinity-purified by using streptavidin-conjugated agarose beads (EZview™ Red Streptavidin Affinity Gel, Sigma-Aldrich). The initial protein peaks containing EP400NL were collected and pooled together after the size exclusion chromatography and incubated with streptavidin affinity beads at 4 °C overnight with constant rotation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasma insulin and C-peptide were measured with mouse/rat ELISAs (EZRMI-13K and EZRMCP2-21K, respectively, EMD Millipore, Billerica, MA, USA). Plasma non-esterified fatty acids (NEFA ...
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bioRxiv - Biophysics 2022Quote: ... about 100 μL supernatant was left and the bound protein was eluted by incubation with 10 μL FLAG peptide (Sigma-Aldrich F3290) at 4°C for 3 hours ...
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bioRxiv - Biochemistry 2021Quote: ... 12 μg peptides were adjusted to pH 7-8 by adding one volume of 50 mM ammonium bicarbonate (ABC, Sigma-Aldrich, A6141). Offline fractionation by high-pH reversed-phase chromatography was carried out on an Ultimate 3000 HPLC (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The source voltage and focusing voltages were tuned for the transmission of MRFA peptide (m/z 524) (Sigma-Aldrich, St. Louis, MO). Data-dependent analysis was carried out in orbitrap-IT parallel mode using CID fragmentation on the five most abundant ions in each cycle ...
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bioRxiv - Biophysics 2020Quote: ... Approximately 10 pmol of peptides were loaded onto the Ascentis Peptides ES-C18 column (2.1 mm x 100 mm, 2.7 μm particle size; Sigma-Aldrich, St. Louis, MO) at 0.400 mL/min flow rate and were eluted with the following gradient ...
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bioRxiv - Genetics 2020Quote: ... The Rad21l1 peptide was concentrated to a final concentration of 1 mg/ml in PBS using a 10 kDa centrifugal filter (Sigma, Catalog # UFC901008). The Rad21l1-derived peptide was injected into three guinea pigs by Pocono Rabbit Farm and Laboratory following the 91-day polyclonal antibody production protocol.
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bioRxiv - Cell Biology 2021Quote: ... The beads were then extensively washed in lysis buffer and elution was carried out with 50 μL of 3×FLAG peptide (Sigma-Aldrich #F4799), the supernatant was separated from the beads by centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... and proteins were eluted with 4 mL of the same buffer but containing 100 μL (5 mg mL−1) Flag peptide (Sigma-Aldrich F4799). The eluate was spin concentrated (Sigma-Aldrich CLS431485-251A ...
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bioRxiv - Neuroscience 2021Quote: ... This antiserum detects human α-CGRP and β-CGRP but does not cross-react with any other peptide (data supplied by Sigma). We used a rabbit polyclonal antibody (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed 3 times with 500 µL of lysis buffer and bound protein was eluted with 50 µl of elution buffer (100 µg/mL FLAG peptide (Sigma-Aldrich #F3290), 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... the complex was collected in buffer containing 0.2 mg/ml FLAG peptide and concentrated to 400 μL in an Amicon® Ultra-15 Centrifugal Filter Unit (Millipore, Burlington, MA). Complexes were loaded onto a Superose 6 Increase 10/300GL column (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... Peptide samples were loaded onto a Sigma-Aldrich Ascentis Peptides ES-C18 column (2.1 mm x 100 mm, 2.7 µm particle size; Sigma-Aldrich, St. Louis, MO) via an Infinity Autosampler (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... and seeded at 1 x 105 in a 24 well plate and then pulsed with 1ng/mL OVA peptide (Sigma, #S7951-01mg) for 3 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... beads washed five times with pre-chilled NET resuspension buffer and eluted under native conditions by competition using 3x FLAG peptide (Millipore Sigma, F4799). Samples were boiled in the Laemmli SDS sample buffer (Thermofisher ...
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bioRxiv - Immunology 2023Quote: ... were cocultured for 6 hours with autologous herpesvirus papio-immortalized B lymphoblastoid cell lines (B-LCLs) pulsed with Nef peptides (Sigma-Aldrich Japan) at 1 μM concentration or as indicated otherwise under GolgiStop (monensin ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged proteins were eluted by addition of 40 µL IP buffer supplemented with 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) and incubated for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 2.5 x 105 splenocytes from OT-1 mice were seeded in one ml of complete media in 24 well plates and 0.5µg/ml of peptide (Sigma-Aldrich, cat. number S7951). After 48 hours blasts were expanded with 100 U/ml of rmIL-2 in fresh media and harvested on day four or six after initial stimulation.
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bioRxiv - Immunology 2023Quote: ... Cells were incubated in the presence of either 1 μg/mL OVA peptide (323-339; pOVA) (Sigma-Alrdrich; St Louis, MO, USA), 2.5 μg/mL concanavalin A (ConA ...
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bioRxiv - Biochemistry 2024Quote: ... the gel was washed with 10 column volumes of lysis buffer and subsequently eluted with 100 µg/mL free Flag peptide (Sigma Aldrich, F3290) in lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody was incubated at room temperature protected from light for 1 hour in Intercept®(TBS) Protein-Free Blocking Buffer with 0.01% Tween®20 (Sigma, St. Louis, MO) using IRDye® 800CW Goat anti-Mouse IgG secondary antibody at 1:10,000 (LI-COR Lincoln ...
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bioRxiv - Cell Biology 2021Quote: ... Primary and secondary antibody incubations were performed for 1 hour at 25°C in blocking buffer with anti-Flag-M1 (Sigma-Aldrich, #F3040, 1:1000) conjugated with Alexa-647 (Molecular Probes ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane is incubated for 1 h in a blocking buffer of PBST (PBS supplemented with 0.1 v/v % Tween) and 10% (v/w) dry skim milk (Sigma-Aldrich, St. Louis, MO, USA). The membrane is washed three times with PBST for 5 min and then incubated for 1 h at 25 C with a primary anti-dynein antibody (sc-13524 ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in blocking solution with the following primary antibody: 1:1000 mouse anti S-100 β subunit (S2532, Sigma-Aldrich, St. Louis, MO). Sections were washed and then incubated for 2h in blocking solution with the following secondary antibody at a 1:500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were further incubated at room temperature for 1 h with a blocking buffer (3% normal donkey serum; S30, Chemicon by EMD Millipore, Billerica, MA, USA) in PBS solution containing 0.2% Triton X-100 and 0.05% Tween 20 (PBST) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... prepared in antibody blocking solution containing 0.5% BSA (Fisher, Pittsburgh PA, USA #BP9703-100) and 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA #T9284) for 48-72 hours at 4°C in a shaker ...
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bioRxiv - Neuroscience 2019Quote: ... following by primary antibody incubation overnight at 4°C (rabbit anti-pRIPK3, Abcam; mouse anti-TH, Millipore; mouse anti-pMLKL: Millipore rabbit anti-TH, Millipore in blocking/permeabilizing solution). Sections were washed in 0.1M TBS and then incubated in secondary antibodies for 2 hours at RT in TBS (goat anti-rabbit Alexa Fluor 488 ...
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bioRxiv - Genomics 2021Quote: ... and nucleolin (D4C7O) rabbit mAb (Cell Signalling cat # 14574) appropriately diluted in Odyssey® Blocking Buffer (TBS) 0.1% TWEEN® 20 (Sigma Aldrich cat # P1379) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... in 50 μL of blocking buffer were added for 1 hour followed by 50 μL ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5000 dilution) in blocking buffer for 1 hour at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: ... and then blocked for 1 hour in blocking buffer (1% BSA and 10% normal donkey serum, 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA). Primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... the calcium free medium was replaced with the cell culture medium with or without E-cadherin blocking monoclonal antibody DECMA-1 (Millipore, 10 µg mL−1), and cells migration was carried out for 4 hours at 37°C in a 5% CO2 humidified incubator.
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bioRxiv - Genetics 2022Quote: ... Membranes were washed three times with TBST and incubated with blocking buffer containing Rabbit IgG HRP Linked Whole Ab (Sigma cat no. GENA934-1ML) at 1:5000 for two hours with gentle mixing ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in primary antibodies (diluted in the blocking solution, see Table 1) against myc (rabbit c-Myc, 1:1000, Sigma-Aldrich, C3956, RRID: AB_439680) and FLAG (mouse-FLAG ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in blocking solution: rabbit anti-GFP (MBL Cat# 598; 1/300) and mouse anti-HA (Sigma, Cat# 12ca5; 1/50). On the next day ...
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bioRxiv - Genetics 2024Quote: ... by transferring them in a small drop of blocking solution to the surface of a 0.01% poly-L-lysine (Sigma Aldrich, Cat# 25988-63-0) coated slide ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated in blocking buffer for 1 hr at room temperature followed by incubation with rabbit anti-RBM10 (Sigma-Aldrich HPA034972, 1:2000), rabbit anti-HDAC1 (GeneTex GTX100513 ...
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bioRxiv - Microbiology 2023Quote: ... After cell permeabilization and blocking with 1% normal goat serum (Nacalai Tesque) in dilution buffer [0.3% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) in PBS containing 3% bovine serum albumin (Nacalai Tesque)] ...
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bioRxiv - Cell Biology 2022Quote: ... Nitrocellulose membranes were blocked for 1 h with the blocking buffer containing 5 % dried milk in TBS-Tween 1 % and incubated over-night with primary antibodies: anti-αTubulin (Sigma, T6074, 1:1000 dilution), anti-acetylated Tubulin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... in 50 μl of blocking buffer were added for 1 h followed by 50 μl ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5.000 dilution) in blocking buffer for 1 h at RT ...