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Citations for Millipore Sigma :
4201 - 4250 of 10000+ citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Tubes were placed in a cylindrical phantom (2 cm diameter, 2 % agarose mixed with 5 % intralipid (Sigma-Aldrich AG, Switzerland). The phantom holder was placed in an imaging chamber filled with water and kept at 28°C.
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bioRxiv - Bioengineering 2020Quote: ... A 2 mm by 2 mm piece of Kim wipe was soaked in 30% Fluoro-Ruby (FR; EMD Millipore, AG335) and placed inside the silicon nerve cuff toward the bottom ...
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bioRxiv - Physiology 2020Quote: ... Protocol 2 (Pilocarpine): Six participants had 2 intradermal microdialysis membranes inserted and perfused with 1.67 mg/ml pilocarpine (Sigma-Aldrich) prior to the same B2R antagonist and agonist approach described in Protocol 1 (Figure 1B) ...
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bioRxiv - Molecular Biology 2021Quote: ... The ionic liquid solution was dialyzed into lysis buffer (6 M urea, 2 M thiourea, 2% CHAPS, 1% DTT) using a 3k MWCO cellulose membrane (Amicon Ultra, Millipore).
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bioRxiv - Bioengineering 2022Quote: ... ALP activity in cell lysates was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Biochemistry 2020Quote: 2-amino-2-hydroxymethyl propane-1,3-diol (Tris BioUltra for molecular biology) was purchased from Sigma-Aldrich (cat. no. 93362). RNA oligonucleotides were purchased from Biomers.net (Ulm ...
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bioRxiv - Microbiology 2020Quote: 2 μg mRNA suspended in 6 μl RNAse-free H2O was digested with 2 units of nuclease P1 (Sigma N8630) for 3 h at 37 ºC ...
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bioRxiv - Microbiology 2020Quote: ... Benztropine mesylate (CAS No. 132-17-2) and Bafilomycin A1 (CAS No. 88899-55-2) were purchased from Sigma-Aldrich. Active pharmaceutical ingredients for an additional 59 drugs ...
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bioRxiv - Biochemistry 2020Quote: ... PCNA-12 co-expression plasmids were constructed by inserting non-tagged PCNA-1 into multiple cloning site 1 and PCNA-2 (without and with a His6-tag) into site 2 of pET-DUET-1 plasmid (Novagen).
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bioRxiv - Biochemistry 2020Quote: ... Day 1 adult animals were mounted on 2% (vol/vol) agar pads and immobilized with 30 mg/mL 2-3-butaneione monoxime (BDM, Sigma) in M9 buffer.
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bioRxiv - Biochemistry 2020Quote: ... animals were mounted on 2% (vol/vol) agar pads and immobilized in 30 mg/mL 2-3-butaneione monoxime (BDM, Sigma) in M9 buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... They were then differentiated into cardiac mesoderm using RPMI B27-medium (RPMI1640 GlutaMAX+ 2% B27 supplement without insulin, 200 µM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate [Sigma] and 1% Penicillin/Streptomycin [all ThermoFisher Scientific unless otherwise indicated] ...
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bioRxiv - Immunology 2022Quote: ... ALP activity was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Cell Biology 2019Quote: ... Central channels were loaded with cells and 2 mg/mL fibrinogen after addition of bovine thrombin (2 U/ml) (Sigma). After polymerization ...
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bioRxiv - Microbiology 2019Quote: ... referred to here as hCMEC were obtained from Millipore and were maintained in an EndoGRO-MV complete medium kit supplemented with 1 ng/ml fibroblast growth factor-2 (FGF-2; Millipore) (81–84) ...
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bioRxiv - Developmental Biology 2019Quote: ... Dechorionated live embryos were incubated for 6, 12 and 24 hours in 40μM Nifedipine or 20mM BDM (2, 3-butanedione-2-monoxime) (Sigma-Aldrich) in Danieau’s solution ...
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bioRxiv - Immunology 2020Quote: ... the membranes were incubated with the appropriate horseradish-peroxidase conjugated secondary Ab (1:5000 in TBST + 2% BSA; 2 h at room temperature; anti-mouse A4416 and anti-rabbit A0545, Sigma). The immunoreactivity was detected by ECL reagents (Amersham) ...
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bioRxiv - Cell Biology 2021Quote: ... and re-suspended in equal volume of 2×Native lysis buffer (200mM Tris pH 7.5, 150mM NaCl, 2mM PMSF, 2×Protease Inhibitor Cocktails (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2021Quote: ... of 2 in the presence of 2.2 mM ferrihydrite (Schwertmann and Fischer, 1973; Stookey, 1970) and 2 mM ferrozine (Sigma-Aldrich). Where indicated ...
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bioRxiv - Neuroscience 2021Quote: ... NEL-MG were treated with 2 μL of red fluorescent latex beads (2 μm, Sigma-Aldrich, St. Louis, MO, USA), HiLyte™ Fluor 488-labeled amyloid β peptide 25-35 (2 μL) ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting pellets were further extracted by sonication in 3X volumes of SDS lysis buffer (50 mM Tris-HCl pH 7.4, 2% SDS, 1X EDTA-free Complete protease inhibitor cocktail [Roche] and 1X phosphatase inhibitor cocktail 2 and 3 [Sigma]). Samples were centrifuged at 21,000g for 30 min at 25°C to obtain the Triton-insoluble (SDS-soluble ...
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bioRxiv - Cell Biology 2021Quote: ... hPSCs were cultured in RPMI B27-medium (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Mesoderm was induced with RPMI B27- (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Skin was minced into small pieces (about 2-3 mm × 2-3 mm) and digested by incubation with 1 mg/mL collagenase V (Sigma) in PBS for one hour with shaking (250 rpm) ...
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bioRxiv - Neuroscience 2021Quote: The following odors/concentrations were used for comparing molecular properties to receptor responses: 1% 2-methyl-2-pentenal (Sigma 294667), 1% trans-cinnamaldehyde (Sigma C80687) ...
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bioRxiv - Immunology 2022Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30 °C for 24 hours at 200rpm ...
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bioRxiv - Bioengineering 2022Quote: ... ALP activity in cell lysates was determined by adding 20 μl of 0.75 M 2-amino-2-methyl-1-propanol (A65182, Sigma-Aldrich) to 80 μl sample in 96-wells assay plates ...
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bioRxiv - Immunology 2022Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30 °C for 24 hours at 200rpm ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A volume of 40 µL of the mix (corresponding to 8.105 cells) was then plated on 6 cm Petri dishes filled with 2 mL of 2% Phytagel (Sigma-Aldrich), following Dubravcic et al (Dubravcic et al. ...
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Optimization and deoptimization of codons in SARS-CoV-2 and the implications for vaccine developmentbioRxiv - Evolutionary Biology 2022Quote: ... About 2×106 cells of HEK293T cells were transfected with 2 ug of plasmids encoding S proteins using polyetherimide (PEI; Sigma). After 16 hours of incubation ...
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bioRxiv - Plant Biology 2022Quote: ... 100 mM ammonium bicarbonate [AB], 10 mM Tris[2-carboxyethyl]phosphine hydrochloride, 50 mM 2-chloroacetamide, 0.2% protease inhibitor [Sigma, P8340]), and incubated in the dark at room temperature for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were placed into 2 ml cold EZ lysis buffer in a 2 ml glass dounce tissue grinder (Sigma, D8938) and homogenized by hand 25 times with pestle A followed by 25 times with pestle B while incorporating a 180-degree twist ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with PBS-T containing 2% NGS and antisera against Olig-2 (1:500,Millipore) A2B5 (which stains for OPCs ...
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bioRxiv - Neuroscience 2022Quote: ... mice were injected with the noradrenergic neuron specific neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP4; Sigma, C8417) once at a dose of 50 mg/kg (i.p.) ...
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bioRxiv - Bioengineering 2022Quote: ... 80 μL of the supernatant was mixed with 20 μL of 0.75M 2-amino-2-methyl-1-propanol buffer (A65182, Sigma-Aldrich) and 100 μL 100 mM p-nitrophenylphosphate solution (71768 ...
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bioRxiv - Molecular Biology 2024Quote: ... and human epithelial type 2 (HEp-2, CCL-23, ATCC) cells were cultured in Dulbecco’s modified eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... in PBS for 2 h at room temperature and counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Sigma-Aldrich) to identify cell nuclei.
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bioRxiv - Genetics 2024Quote: ... acr-2(gf) double mutants were washed into a 2 mL solution of M9 media with 47 mM ethyl methanesulfonate (Sigma) and incubated at 22°C on a spinning wheel for 4 hours ...
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bioRxiv - Immunology 2024Quote: T cell clones were rested overnight in fresh media without IL-2 and added to wells containing matured DCs at 1:1 ratio in the presence of monensin (2 uM, Sigma) and Brefeldin A (5 ug/ml ...
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bioRxiv - Immunology 2023Quote: Duodenal biopsies were collected in ice-cold RPMI-1640 and processed into single- cell suspensions by treatment with 2 mM EDTA in 2% (v/v) FBS/PBS followed by digestion with collagenase (Sigma) and cryopreservation as previously described.22 PBMCs were isolated from peripheral blood by density gradient centrifugation followed by cryopreservation ...
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bioRxiv - Neuroscience 2023Quote: ... the dyes were removed by flowing 2.5 mL of cleavage buffer comprising 2× SSC and 50 mM of Tris (2-carboxyethyl) phosphine (TCEP; Sigma, 646547) with 15 min incubation in the flow chamber to cleave the dyes linked to the readout probes through disulfide bond ...
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bioRxiv - Neuroscience 2022Quote: ... or DMSO 0.1% as vehicle was evaluated using a MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) assay as described in (Sanz et al. ...
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bioRxiv - Neuroscience 2023Quote: The mice received three injections every 2 h (200 ul/injection, intraperitoneally in alternative sites of the body) of 5-Chloro-2’-deoxy-Uridine (CldU, Sigma reference C6891 ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then blocked 1 hr at the room temperature (RT) in PBS with 2% Triton-X100 and 2% normal donkey serum (Sigma), followed by an incubation overnight at 4℃ with primary antibody diluted in the blocking solution ...
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bioRxiv - Microbiology 2023Quote: ... the reaction was stopped by FBS then cell were washed 2 time with PBS-based staining buffer containing 2% fetal bovine serum (FBS) and 0.01% NaN3 (Sigma-Aldrich), blocked Fc IgG receptors by pre-incubated with CD16/32(93 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown overnight in SC-URA medium with 2% dextrose and were plated in SC-URA plates in the presence of 2% dextrose (Sigma) or 2% galactose (Difco) ...
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bioRxiv - Biochemistry 2022Quote: Phosphate buffer saline solution (Ca 2+, Mg 2+ free) was prepared using Phosphate Buffer Saline (PBS) powder (Sigma Aldrich, USA.). 9.6 grams of the powdered reagent was added to 990 milliliters of ultra-purified milli-Q water (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... permeabilised by washing in 0.1% Triton-X in PBS (PBS-T) for 15 min and incubated for 2 h in a blocking buffer of 2% bovine serum albumin (Sigma) and 3% normal donkey serum (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... (Catalog No: PCT0809, Himedia), 10 mM of 2-Naphthaleneacetic acid (2-NAA, inactive form of auxin) (Catalog No: N4002, Sigma) and 20 nM of synthetic auxin 2,4-Dichlorophenoxyacetic acid (2,4-D ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected with 2 µg of scrambled siRNA (MISSION siRNA Universal Negative Control #2 Sigma-Aldrich SIC002-5x1nmol) or 2 µg of MATR3 siRNA (MISSION esiRNA human MATR3 Sigma-Aldrich EHU113801-50UG ...